Mutagenetix > Incidental Mutation

Incidental Mutation 'R8373:Prss3b'

646536

Institutional Source

Beutler Lab

Gene Symbol

Prss3b

Ensembl Gene

ENSMUSG00000029882

Gene Name

serine protease 3B

Synonyms

2210010C04Rik

MMRRC Submission

067741-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R8373 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

41007202-41012443 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 41008622 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 231 (T231P)

Ref Sequence

ENSEMBL: ENSMUSP00000031931 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031931]

AlphaFold

Q9CPN9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000031931
AA Change: T231P

PolyPhen 2 Score 0.532 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000031931
Gene: ENSMUSG00000029882
AA Change: T231P

Domain	Start	End	E-Value	Type
Tryp_SPc	24	240	3.52e-102	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg6	T	C	10: 14,343,078 (GRCm39)	T290A	probably benign	Het
Aff4	C	T	11: 53,291,094 (GRCm39)	Q685*	probably null	Het
Ankmy1	A	G	1: 92,823,816 (GRCm39)	M150T	probably damaging	Het
Armc1	T	C	3: 19,203,731 (GRCm39)	Y65C	probably damaging	Het
Armc8	A	G	9: 99,409,152 (GRCm39)	V222A	probably benign	Het
Bcar1	A	T	8: 112,442,370 (GRCm39)	Y228*	probably null	Het
Cct2	T	C	10: 116,896,729 (GRCm39)	D158G	possibly damaging	Het
Cul1	T	A	6: 47,491,997 (GRCm39)	C426S	possibly damaging	Het
Deup1	G	T	9: 15,503,671 (GRCm39)	L297M	possibly damaging	Het
Dpp10	A	G	1: 123,781,958 (GRCm39)	S74P	possibly damaging	Het
Epha4	A	G	1: 77,483,716 (GRCm39)	Y98H	possibly damaging	Het
Epn3	T	G	11: 94,383,762 (GRCm39)	D296A	probably damaging	Het
Eri2	A	C	7: 119,371,820 (GRCm39)	I252S	probably benign	Het
Gp9	C	T	6: 87,755,994 (GRCm39)	T3I	probably benign	Het
H2-Q5	T	A	17: 35,613,432 (GRCm39)	V55E		Het
Kif3c	G	T	12: 3,416,089 (GRCm39)	V37L	probably benign	Het
Lct	A	T	1: 128,231,577 (GRCm39)	N757K	probably damaging	Het
Lhx8	A	C	3: 154,030,295 (GRCm39)	N112K	probably damaging	Het
Loxl3	T	A	6: 83,025,872 (GRCm39)	S373R	possibly damaging	Het
Mettl4	G	A	17: 95,041,077 (GRCm39)	T359I	probably damaging	Het
Mpp7	A	G	18: 7,444,096 (GRCm39)	S109P	probably damaging	Het
Nckap5	A	G	1: 125,954,032 (GRCm39)	V840A	probably benign	Het
Ncoa4	T	A	14: 31,898,893 (GRCm39)	L571Q	probably damaging	Het
Or13a18	A	T	7: 140,190,208 (GRCm39)	Y35F	possibly damaging	Het
Or2f1	T	C	6: 42,721,280 (GRCm39)	F103S	probably damaging	Het
Phgdh	G	A	3: 98,228,561 (GRCm39)	T204I	probably damaging	Het
Pla2g4d	C	T	2: 120,107,980 (GRCm39)	V310M	probably null	Het
Psmd12	C	T	11: 107,388,450 (GRCm39)	P421L	probably damaging	Het
Ptch1	A	G	13: 63,688,982 (GRCm39)	Y432H	probably damaging	Het
Rapgef1	G	A	2: 29,600,243 (GRCm39)	G655S	probably damaging	Het
Rilpl2	C	A	5: 124,616,097 (GRCm39)	A18S	probably damaging	Het
Srsf7	T	C	17: 80,512,815 (GRCm39)	R88G	probably benign	Het
St6galnac1	T	C	11: 116,660,059 (GRCm39)	K85E	possibly damaging	Het
Trip12	A	T	1: 84,773,488 (GRCm39)	S49R	probably damaging	Het
Vinac1	T	A	2: 128,880,091 (GRCm39)	I612F		Het
Wdr48	A	G	9: 119,734,560 (GRCm39)	T160A	probably damaging	Het
Zfp704	G	A	3: 9,674,502 (GRCm39)	T93M	unknown	Het

Other mutations in Prss3b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01120:Prss3b	APN	6	41,008,607 (GRCm39)	missense	probably damaging	1.00
IGL01625:Prss3b	APN	6	41,009,882 (GRCm39)	missense	probably benign	0.01
IGL02159:Prss3b	APN	6	41,009,891 (GRCm39)	missense	probably benign	0.34
IGL03161:Prss3b	APN	6	41,011,240 (GRCm39)	missense	probably damaging	0.96
R0419:Prss3b	UTSW	6	41,011,281 (GRCm39)	missense	probably benign	0.05
R1715:Prss3b	UTSW	6	41,009,870 (GRCm39)	splice site	probably null
R2057:Prss3b	UTSW	6	41,009,315 (GRCm39)	missense	probably benign	0.06
R2059:Prss3b	UTSW	6	41,009,315 (GRCm39)	missense	probably benign	0.06
R2136:Prss3b	UTSW	6	41,012,396 (GRCm39)	missense	probably benign	0.28
R2446:Prss3b	UTSW	6	41,008,582 (GRCm39)	missense	probably benign	0.15
R4862:Prss3b	UTSW	6	41,009,345 (GRCm39)	missense	possibly damaging	0.74
R5068:Prss3b	UTSW	6	41,009,370 (GRCm39)	missense	probably benign	0.35
R5369:Prss3b	UTSW	6	41,009,940 (GRCm39)	missense	probably benign	0.00
R6392:Prss3b	UTSW	6	41,009,306 (GRCm39)	missense	probably damaging	1.00
R7010:Prss3b	UTSW	6	41,009,247 (GRCm39)	missense	probably benign	0.42
R7567:Prss3b	UTSW	6	41,010,127 (GRCm39)	missense	probably benign	0.04
R7727:Prss3b	UTSW	6	41,010,127 (GRCm39)	missense	probably benign	0.04
R7962:Prss3b	UTSW	6	41,012,387 (GRCm39)	missense	probably benign	0.01
R8529:Prss3b	UTSW	6	41,009,369 (GRCm39)	missense	probably benign	0.00
R8883:Prss3b	UTSW	6	41,009,305 (GRCm39)	nonsense	probably null
R8916:Prss3b	UTSW	6	41,010,103 (GRCm39)	missense	probably damaging	1.00
R9066:Prss3b	UTSW	6	41,008,640 (GRCm39)	missense	probably benign	0.41
R9095:Prss3b	UTSW	6	41,010,038 (GRCm39)	missense	possibly damaging	0.76
R9180:Prss3b	UTSW	6	41,008,681 (GRCm39)	missense	probably damaging	1.00
R9389:Prss3b	UTSW	6	41,010,079 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GGCAGCAATTTCAAGAGAAAGTTC -3'
(R):5'- ACCTACAAAAGTGGCCCGAG -3'

Sequencing Primer
(F):5'- GTTCGTTATTATGCAAGAACCATTC -3'
(R):5'- TGGCCCGAGATAATCAAGTAAC -3'

Posted On

2020-09-02