Mutagenetix > Incidental Mutations

Incidental Mutation 'R8386:Zfp882'

647162

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.244) question?

Stock #

R8386 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

71908608-71916354 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 71914118 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Arginine at position 263 (H263R)

Ref Sequence

ENSEMBL: ENSMUSP00000105629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

probably benign
Transcript: ENSMUST00000110002
AA Change: H263R

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: H263R

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125802

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000126607

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700029P11Rik	C	T	15: 81,980,629	L24F	probably damaging	Het
Abca9	G	T	11: 110,130,692	A1064D	probably damaging	Het
Adgb	A	G	10: 10,350,304	I1390T	probably damaging	Het
Aktip	G	A	8: 91,131,046	R13C	probably benign	Het
Arnt2	T	A	7: 84,347,539	I71F	probably damaging	Het
Casr	T	A	16: 36,515,588	I120F	probably damaging	Het
Cilp	G	A	9: 65,279,004	G794S	probably damaging	Het
Cntnap1	A	T	11: 101,182,203	R544S	probably damaging	Het
Cse1l	T	C	2: 166,919,684	S53P	probably benign	Het
Cyfip1	T	C	7: 55,877,740	S207P	probably damaging	Het
Fam120b	G	A	17: 15,422,984	S675N	probably benign	Het
Fcna	C	T	2: 25,626,015	W163*	probably null	Het
Gast	A	G	11: 100,336,865	D91G	probably damaging	Het
Igkv6-14	T	C	6: 70,434,982	Y106C	probably damaging	Het
Lin28b	A	G	10: 45,469,044	V62A	probably damaging	Het
Malrd1	T	A	2: 15,696,844	S757T	unknown	Het
Ms4a15	A	T	19: 10,993,182	C3S	unknown	Het
Muc5ac	G	A	7: 141,807,634	V1561I	possibly damaging	Het
Olfr1205	G	A	2: 88,831,289	M57I	possibly damaging	Het
Olfr495	TTTCTTCTTCT	TTTCTTCT	7: 108,395,348		probably benign	Het
Pi16	C	A	17: 29,319,234	P7Q	possibly damaging	Het
Ppp1r3c	T	C	19: 36,733,938	D144G	probably damaging	Het
Setd1b	T	A	5: 123,144,256	V149D	unknown	Het
Shcbp1	A	C	8: 4,767,951	W115G	probably damaging	Het
Shroom1	A	T	11: 53,466,403	K631M	probably damaging	Het
Sipa1l2	A	T	8: 125,492,093	S168R	probably damaging	Het
Slc12a4	A	T	8: 105,951,618	N314K	probably damaging	Het
Syngap1	T	A	17: 26,960,491	M755K	possibly damaging	Het
Tnik	A	G	3: 28,263,674	D11G	unknown	Het
Trim80	A	G	11: 115,445,074	D274G	probably damaging	Het
Ush2a	A	G	1: 188,728,206	T2555A	possibly damaging	Het
Vps13a	A	T	19: 16,701,119		probably null	Het
Wnt7a	C	T	6: 91,366,288	G204D	probably damaging	Het
Zfp420	A	T	7: 29,875,618	Y421F	probably benign	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	71913827	missense	probably benign
R0244:Zfp882	UTSW	8	71913523	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	71914615	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	71914337	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	71914686	nonsense	probably null
R1299:Zfp882	UTSW	8	71913473	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	71913609	missense	probably damaging	0.97
R4829:Zfp882	UTSW	8	71914389	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	71914654	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	71914360	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	71913459	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	71913155	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	71914505	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	71914640	missense	probably damaging	1.00
R6888:Zfp882	UTSW	8	71914286	missense	probably benign	0.01
R6987:Zfp882	UTSW	8	71914673	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	71913249	critical splice donor site	probably null
R7780:Zfp882	UTSW	8	71914229	missense	possibly damaging	0.89
R7793:Zfp882	UTSW	8	71913141	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTCTTGCTGGTATTCAACAGCAC -3'
(R):5'- CCCTGTGAATCTGTTCATGGTATC -3'

Sequencing Primer
(F):5'- GGCAAAACCTTCACTTATTCCAGTGG -3'
(R):5'- GTGATGCATGAAGGCTTTCCCAC -3'

Posted On

2020-09-02