Mutagenetix > Incidental Mutation

Incidental Mutation 'R8387:Vmn2r77'

647205

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r77

Ensembl Gene

ENSMUSG00000090949

Gene Name

vomeronasal 2, receptor 77

Synonyms

EG546983

MMRRC Submission

067876-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R8387 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

86444349-86461240 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 86450947 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Lysine at position 278 (Q278K)

Ref Sequence

ENSEMBL: ENSMUSP00000129540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164996]

AlphaFold

L7N2B7

Predicted Effect

probably benign
Transcript: ENSMUST00000164996
AA Change: Q278K

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000129540
Gene: ENSMUSG00000090949
AA Change: Q278K

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	467	1.4e-30	PFAM
Pfam:NCD3G	510	562	1e-20	PFAM
Pfam:7tm_3	594	830	2.6e-52	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (46/47)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1b	G	A	5: 8,874,698 (GRCm39)	D453N	probably damaging	Het
Arl14ep	T	C	2: 106,799,562 (GRCm39)	D93G	probably damaging	Het
Bcl7b	T	C	5: 135,197,413 (GRCm39)	I18T	probably damaging	Het
Cdh1	A	G	8: 107,390,501 (GRCm39)	I614V	probably benign	Het
Cpa3	T	C	3: 20,281,400 (GRCm39)	I169V	probably benign	Het
Cpeb4	T	C	11: 31,858,877 (GRCm39)		probably null	Het
Csmd1	G	A	8: 16,050,484 (GRCm39)	H2251Y	possibly damaging	Het
Dab2ip	T	A	2: 35,609,870 (GRCm39)	I695K	probably damaging	Het
Dhtkd1	C	A	2: 5,934,479 (GRCm39)	L230F	possibly damaging	Het
Emc1	T	C	4: 139,088,600 (GRCm39)	S353P	probably benign	Het
Erc2	T	A	14: 27,375,253 (GRCm39)	L157Q	possibly damaging	Het
Flvcr1	A	G	1: 190,743,731 (GRCm39)		probably null	Het
Fryl	A	C	5: 73,293,663 (GRCm39)		probably null	Het
Gnl2	T	C	4: 124,949,127 (GRCm39)	*729Q	probably null	Het
Gpr63	G	A	4: 25,008,301 (GRCm39)	V342M	possibly damaging	Het
Guf1	C	T	5: 69,723,810 (GRCm39)	P463L	probably damaging	Het
Hmmr	G	A	11: 40,612,499 (GRCm39)	S206F	probably damaging	Het
Ifngr2	T	C	16: 91,358,535 (GRCm39)	L245P	probably damaging	Het
Igsf10	A	G	3: 59,236,564 (GRCm39)	F1206L	probably damaging	Het
Ktn1	T	A	14: 47,944,744 (GRCm39)		probably null	Het
Lekr1	A	G	3: 65,591,520 (GRCm39)	K86E	possibly damaging	Het
Lrrc3	C	T	10: 77,737,346 (GRCm39)	G30D	possibly damaging	Het
Mapk8ip2	T	C	15: 89,344,897 (GRCm39)	F765L	probably damaging	Het
Myo6	A	G	9: 80,183,632 (GRCm39)	T676A	unknown	Het
Nr4a1	T	C	15: 101,171,053 (GRCm39)	S510P	probably damaging	Het
Or2o1	T	C	11: 49,051,497 (GRCm39)	S219P	probably damaging	Het
Or4c113	T	C	2: 88,885,646 (GRCm39)	I41M	probably benign	Het
Or4c11c	G	A	2: 88,661,633 (GRCm39)	M57I	possibly damaging	Het
Or51e1	C	T	7: 102,359,402 (GRCm39)	T312I	probably benign	Het
Or5a1	A	G	19: 12,097,785 (GRCm39)	L97P	probably damaging	Het
Pdcd1	G	A	1: 93,969,193 (GRCm39)	L42F	probably damaging	Het
Pdzd7	T	C	19: 45,018,490 (GRCm39)	D621G	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Pias4	G	A	10: 80,990,342 (GRCm39)	R398C	probably benign	Het
Plekha6	A	T	1: 133,219,893 (GRCm39)		probably null	Het
Prkag3	A	G	1: 74,784,854 (GRCm39)		probably null	Het
Ptpn7	A	G	1: 135,061,606 (GRCm39)	T23A	probably benign	Het
Ptprd	T	C	4: 75,873,526 (GRCm39)	D1069G	probably damaging	Het
Ptprr	A	G	10: 116,087,030 (GRCm39)	Y503C	probably damaging	Het
Slc26a8	T	C	17: 28,866,899 (GRCm39)	D610G	probably benign	Het
Smyd4	A	G	11: 75,292,984 (GRCm39)	N638S	probably benign	Het
Tenm3	A	G	8: 48,740,883 (GRCm39)	F1200S	probably damaging	Het
Tox3	A	G	8: 90,984,595 (GRCm39)	S195P	probably benign	Het
Trim44	T	C	2: 102,230,518 (GRCm39)	E171G	probably damaging	Het
Vars1	T	A	17: 35,229,490 (GRCm39)	M369K	probably damaging	Het
Zfp345	T	C	2: 150,314,740 (GRCm39)	T266A	probably damaging	Het

Other mutations in Vmn2r77

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00954:Vmn2r77	APN	7	86,449,975 (GRCm39)	missense	probably benign	0.06
IGL01105:Vmn2r77	APN	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
IGL01367:Vmn2r77	APN	7	86,461,124 (GRCm39)	missense	probably damaging	0.98
IGL01634:Vmn2r77	APN	7	86,460,857 (GRCm39)	missense	probably benign
IGL01805:Vmn2r77	APN	7	86,460,395 (GRCm39)	missense	probably benign	0.18
IGL01868:Vmn2r77	APN	7	86,452,224 (GRCm39)	missense	probably benign	0.00
IGL01980:Vmn2r77	APN	7	86,450,678 (GRCm39)	missense	probably benign	0.14
IGL02055:Vmn2r77	APN	7	86,450,763 (GRCm39)	missense	probably benign	0.00
IGL02066:Vmn2r77	APN	7	86,452,836 (GRCm39)	nonsense	probably null
IGL02185:Vmn2r77	APN	7	86,444,360 (GRCm39)	missense	unknown
IGL02200:Vmn2r77	APN	7	86,451,187 (GRCm39)	missense	probably benign	0.04
IGL02336:Vmn2r77	APN	7	86,451,224 (GRCm39)	missense	probably damaging	0.99
IGL02445:Vmn2r77	APN	7	86,452,848 (GRCm39)	nonsense	probably null
IGL02557:Vmn2r77	APN	7	86,444,342 (GRCm39)	unclassified	probably benign
IGL02659:Vmn2r77	APN	7	86,449,979 (GRCm39)	missense	probably benign	0.32
IGL02978:Vmn2r77	APN	7	86,460,555 (GRCm39)	missense	probably benign
IGL03180:Vmn2r77	APN	7	86,450,843 (GRCm39)	missense	possibly damaging	0.85
IGL03255:Vmn2r77	APN	7	86,461,131 (GRCm39)	missense	probably benign	0.04
IGL03273:Vmn2r77	APN	7	86,460,494 (GRCm39)	missense	probably damaging	0.99
R0046:Vmn2r77	UTSW	7	86,451,146 (GRCm39)	missense	possibly damaging	0.73
R0047:Vmn2r77	UTSW	7	86,460,858 (GRCm39)	missense	probably benign	0.01
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0389:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R0635:Vmn2r77	UTSW	7	86,460,383 (GRCm39)	missense	probably benign
R0689:Vmn2r77	UTSW	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
R0827:Vmn2r77	UTSW	7	86,451,224 (GRCm39)	missense	probably damaging	1.00
R1167:Vmn2r77	UTSW	7	86,450,954 (GRCm39)	missense	probably benign	0.02
R1228:Vmn2r77	UTSW	7	86,450,242 (GRCm39)	critical splice donor site	probably null
R1353:Vmn2r77	UTSW	7	86,451,394 (GRCm39)	missense	probably benign	0.29
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1613:Vmn2r77	UTSW	7	86,460,356 (GRCm39)	missense	probably damaging	1.00
R1654:Vmn2r77	UTSW	7	86,461,123 (GRCm39)	missense	probably damaging	1.00
R1742:Vmn2r77	UTSW	7	86,444,543 (GRCm39)	missense	probably benign	0.35
R1827:Vmn2r77	UTSW	7	86,450,821 (GRCm39)	missense	probably damaging	0.99
R1911:Vmn2r77	UTSW	7	86,461,001 (GRCm39)	missense	probably damaging	1.00
R1974:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R2008:Vmn2r77	UTSW	7	86,450,921 (GRCm39)	missense	probably benign	0.31
R2093:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R2143:Vmn2r77	UTSW	7	86,461,152 (GRCm39)	missense	probably damaging	1.00
R2269:Vmn2r77	UTSW	7	86,460,897 (GRCm39)	missense	probably benign	0.03
R2972:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R2974:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R3037:Vmn2r77	UTSW	7	86,450,191 (GRCm39)	missense	probably benign
R3694:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3695:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3805:Vmn2r77	UTSW	7	86,444,368 (GRCm39)	nonsense	probably null
R3870:Vmn2r77	UTSW	7	86,461,050 (GRCm39)	missense	probably damaging	1.00
R4732:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R4733:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R5009:Vmn2r77	UTSW	7	86,451,015 (GRCm39)	missense	possibly damaging	0.82
R5201:Vmn2r77	UTSW	7	86,460,846 (GRCm39)	missense	probably damaging	0.98
R5218:Vmn2r77	UTSW	7	86,451,341 (GRCm39)	missense	probably damaging	0.98
R5469:Vmn2r77	UTSW	7	86,451,271 (GRCm39)	missense	probably benign	0.01
R5673:Vmn2r77	UTSW	7	86,461,214 (GRCm39)	missense	probably benign	0.05
R5771:Vmn2r77	UTSW	7	86,461,235 (GRCm39)	missense	probably benign	0.06
R5832:Vmn2r77	UTSW	7	86,460,670 (GRCm39)	nonsense	probably null
R5899:Vmn2r77	UTSW	7	86,460,924 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn2r77	UTSW	7	86,450,878 (GRCm39)	missense	probably benign	0.00
R6182:Vmn2r77	UTSW	7	86,460,957 (GRCm39)	missense	probably damaging	1.00
R6326:Vmn2r77	UTSW	7	86,451,031 (GRCm39)	missense	probably benign
R6419:Vmn2r77	UTSW	7	86,460,767 (GRCm39)	missense	probably damaging	0.99
R6549:Vmn2r77	UTSW	7	86,450,065 (GRCm39)	missense	probably benign	0.06
R6874:Vmn2r77	UTSW	7	86,451,286 (GRCm39)	missense	probably benign	0.00
R6972:Vmn2r77	UTSW	7	86,452,202 (GRCm39)	missense	probably damaging	1.00
R7056:Vmn2r77	UTSW	7	86,451,023 (GRCm39)	missense	probably benign	0.06
R7185:Vmn2r77	UTSW	7	86,451,035 (GRCm39)	missense	probably benign	0.00
R7261:Vmn2r77	UTSW	7	86,460,518 (GRCm39)	nonsense	probably null
R7298:Vmn2r77	UTSW	7	86,449,979 (GRCm39)	missense	probably benign	0.00
R7662:Vmn2r77	UTSW	7	86,460,492 (GRCm39)	nonsense	probably null
R8182:Vmn2r77	UTSW	7	86,460,801 (GRCm39)	missense	probably damaging	1.00
R8327:Vmn2r77	UTSW	7	86,450,680 (GRCm39)	missense	probably benign	0.08
R8825:Vmn2r77	UTSW	7	86,452,855 (GRCm39)	missense	probably benign
R8898:Vmn2r77	UTSW	7	86,444,430 (GRCm39)	missense	probably damaging	1.00
R8973:Vmn2r77	UTSW	7	86,452,150 (GRCm39)	missense	possibly damaging	0.93
R9258:Vmn2r77	UTSW	7	86,452,302 (GRCm39)	missense	possibly damaging	0.88
R9338:Vmn2r77	UTSW	7	86,460,994 (GRCm39)	missense	probably damaging	1.00
R9358:Vmn2r77	UTSW	7	86,452,236 (GRCm39)	missense	probably benign	0.00
R9377:Vmn2r77	UTSW	7	86,444,442 (GRCm39)	missense	probably benign	0.05
R9404:Vmn2r77	UTSW	7	86,451,247 (GRCm39)	missense	probably benign
R9673:Vmn2r77	UTSW	7	86,450,171 (GRCm39)	missense	possibly damaging	0.75
R9679:Vmn2r77	UTSW	7	86,460,741 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- ACTTCAGATGGAACTGGGTAGG -3'
(R):5'- TGTAGTTAGAAGGGTTCACTCTCTG -3'

Sequencing Primer
(F):5'- CTGGGTAGGAGTCATCATTACAG -3'
(R):5'- GAAGGGTTCACTCTCTGAATAAAGTG -3'

Posted On

2020-09-02