Mutagenetix > Incidental Mutations

Incidental Mutation 'R8399:Vmn1r49'

647759

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r49

Ensembl Gene

ENSMUSG00000095932

Gene Name

vomeronasal 1, receptor 49

Synonyms

VRi2, V1rb2, V1r5

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.179) question?

Stock #

R8399 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

90071893-90078506 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 90072707 bp

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 104 (C104*)

Ref Sequence

ENSEMBL: ENSMUSP00000145107 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071865] [ENSMUST00000203791] [ENSMUST00000226368]

Predicted Effect

probably null
Transcript: ENSMUST00000071865
AA Change: C104*

SMART Domains

Protein: ENSMUSP00000071762
Gene: ENSMUSG00000095932
AA Change: C104*

Domain	Start	End	E-Value	Type
transmembrane domain	15	37	N/A	INTRINSIC
Pfam:V1R	38	302	5e-165	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000203791
AA Change: C104*

SMART Domains

Protein: ENSMUSP00000145107
Gene: ENSMUSG00000095932
AA Change: C104*

Domain	Start	End	E-Value	Type
transmembrane domain	15	37	N/A	INTRINSIC
Pfam:V1R	38	302	5e-165	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000226368
AA Change: C104*

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a non-functional knock-in allele display abnormal patterns of axonal projections in the accessory olfactory bulb. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrv1	A	T	13: 81,489,170	V3384D	possibly damaging	Het
Apba3	C	A	10: 81,268,998	T35N	probably benign	Het
Bcl6	T	C	16: 23,972,948	M219V	probably benign	Het
Bco2	T	A	9: 50,541,118	T217S	probably benign	Het
C1s1	C	T	6: 124,535,293	V277I	probably benign	Het
Ccdc162	T	A	10: 41,539,521	R2149W	probably damaging	Het
Cers6	T	C	2: 68,861,771	F46L	probably benign	Het
Chpf	T	C	1: 75,476,220	I359V	probably benign	Het
Chrnb4	A	C	9: 55,043,823	L52R	probably benign	Het
Cox6a1	T	C	5: 115,345,899	T95A	probably damaging	Het
Def8	T	A	8: 123,455,499	Y197*	probably null	Het
Dmbt1	C	G	7: 131,082,587	D778E	unknown	Het
Dnaaf3	C	T	7: 4,523,937		probably null	Het
Dnm1l	T	A	16: 16,321,672	H484L	probably damaging	Het
Eml1	G	A	12: 108,538,131	S783N	possibly damaging	Het
Frmd4a	T	C	2: 4,572,433	S367P	probably damaging	Het
Hck	A	C	2: 153,138,317	K355N	probably damaging	Het
Hexim2	G	T	11: 103,138,503	R127L	probably damaging	Het
Hivep2	T	C	10: 14,132,434	L1592P	possibly damaging	Het
Htr1d	G	A	4: 136,443,375	G305E	probably damaging	Het
Ifi207	G	A	1: 173,730,278	S298L	unknown	Het
Ighv1-11	A	G	12: 114,612,427	V56A	possibly damaging	Het
Ighv7-1	G	A	12: 113,896,912	T12I	unknown	Het
Kctd14	T	C	7: 97,457,604	L22P	probably damaging	Het
Klra17	T	C	6: 129,874,937		probably benign	Het
Kndc1	C	T	7: 139,913,518	R467W	probably damaging	Het
Maf	T	A	8: 115,706,512	I118F	unknown	Het
Mppe1	T	C	18: 67,225,875	T341A	probably benign	Het
Mtmr2	T	A	9: 13,792,067	V186E	probably benign	Het
Nedd9	G	T	13: 41,318,474	Y176*	probably null	Het
Olfr1145	A	C	2: 87,810,224	M135L	probably damaging	Het
Olfr1238	T	C	2: 89,406,684	T132A	probably benign	Het
Omd	A	G	13: 49,589,869	I132V	possibly damaging	Het
Pcnx4	T	A	12: 72,574,211	M935K	probably benign	Het
Pecr	G	A	1: 72,267,465	T219I	probably benign	Het
Pkd1l3	C	T	8: 109,623,888	P455L	possibly damaging	Het
Plcg2	T	C	8: 117,596,362	Y719H	probably damaging	Het
Plk4	C	T	3: 40,808,830	R479*	probably null	Het
Pms1	T	A	1: 53,267,932		probably null	Het
Raph1	C	T	1: 60,489,318	S928N	unknown	Het
Rtp4	G	T	16: 23,520,414		probably benign	Het
Skor1	C	T	9: 63,145,158	V510I	possibly damaging	Het
Smg1	T	C	7: 118,190,571	T699A	unknown	Het
Tcp11l1	T	C	2: 104,685,375	D381G	probably benign	Het
Tnik	T	A	3: 28,494,010	M56K	unknown	Het
Trappc9	G	A	15: 73,052,282	R204C	probably damaging	Het
Trmt10b	A	T	4: 45,305,870	M184L	possibly damaging	Het
Vmn1r224	T	A	17: 20,419,749	I196N	probably damaging	Het
Vmn2r35	A	C	7: 7,816,898	S124R	probably benign	Het
Wdr62	T	C	7: 30,258,061	E547G	probably damaging	Het
Zfp68	A	T	5: 138,607,820	D80E	probably benign	Het

Other mutations in Vmn1r49

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01293:Vmn1r49	APN	6	90072412	missense	probably damaging	0.99
IGL03230:Vmn1r49	APN	6	90072668	missense	probably damaging	1.00
IGL03308:Vmn1r49	APN	6	90072359	missense	possibly damaging	0.67
R0517:Vmn1r49	UTSW	6	90072738	missense	probably damaging	0.98
R0638:Vmn1r49	UTSW	6	90072666	missense	possibly damaging	0.95
R1498:Vmn1r49	UTSW	6	90072316	missense	probably damaging	1.00
R1649:Vmn1r49	UTSW	6	90072641	missense	possibly damaging	0.66
R1668:Vmn1r49	UTSW	6	90072782	missense	probably benign	0.07
R1816:Vmn1r49	UTSW	6	90072803	missense	possibly damaging	0.93
R2071:Vmn1r49	UTSW	6	90072202	missense	probably benign	0.00
R2155:Vmn1r49	UTSW	6	90072459	missense	probably damaging	0.99
R2274:Vmn1r49	UTSW	6	90072144	missense	probably benign	0.23
R4222:Vmn1r49	UTSW	6	90072246	missense	probably benign	0.06
R4614:Vmn1r49	UTSW	6	90072552	missense	probably benign	0.00
R4797:Vmn1r49	UTSW	6	90072630	missense	probably benign	0.13
R4978:Vmn1r49	UTSW	6	90072890	missense	probably benign	0.30
R5227:Vmn1r49	UTSW	6	90072771	missense	probably benign	0.00
R5959:Vmn1r49	UTSW	6	90072804	missense	probably damaging	1.00
R6741:Vmn1r49	UTSW	6	90072213	missense	probably benign	0.19
R6744:Vmn1r49	UTSW	6	90072202	missense	probably benign	0.00
R7173:Vmn1r49	UTSW	6	90072268	missense	possibly damaging	0.96
R7457:Vmn1r49	UTSW	6	90072552	missense	probably benign	0.00
R7573:Vmn1r49	UTSW	6	90072861	missense	probably benign	0.06
R8360:Vmn1r49	UTSW	6	90073009	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- ACCTGCTTTCTCTGGAGTAAC -3'
(R):5'- GCTCCTTGGTGAGAACAAGC -3'

Sequencing Primer
(F):5'- TCTCTGGAGTAACTCATGGGTAGAAG -3'
(R):5'- TCCTTGGTGAGAACAAGCCTAAAC -3'

Posted On

2020-09-02