Mutagenetix > Incidental Mutation

Incidental Mutation 'R7914:Vmn2r2'

647951

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r2

Ensembl Gene

ENSMUSG00000043897

Gene Name

vomeronasal 2, receptor 2

Synonyms

MMRRC Submission

045962-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.108) question?

Stock #

R7914 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64022699-64049349 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 64041526 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 396 (D396E)

Ref Sequence

ENSEMBL: ENSMUSP00000135110 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077958] [ENSMUST00000177151]

AlphaFold

L7N2E9

Predicted Effect

probably benign
Transcript: ENSMUST00000077958
AA Change: D312E

PolyPhen 2 Score 0.104 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000077109
Gene: ENSMUSG00000043897
AA Change: D312E

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	4.5e-80	PFAM
Pfam:NCD3G	458	511	8.3e-18	PFAM
Pfam:7tm_3	542	779	1.3e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000177151
AA Change: D396E

PolyPhen 2 Score 0.199 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000135110
Gene: ENSMUSG00000043897
AA Change: D396E

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	83	500	1.6e-84	PFAM
Pfam:NCD3G	542	595	2.6e-17	PFAM
Pfam:7tm_3	628	862	1.9e-47	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017N19Rik	G	T	10: 100,428,538 (GRCm39)	V12F	probably benign	Het
Adnp2	T	A	18: 80,174,056 (GRCm39)	R118W	probably damaging	Het
Apaf1	G	A	10: 90,896,095 (GRCm39)	R337C	probably damaging	Het
Arhgef40	T	C	14: 52,225,032 (GRCm39)	L59P	probably damaging	Het
B020011L13Rik	A	G	1: 117,729,162 (GRCm39)	E223G	probably benign	Het
Bcl6	T	C	16: 23,788,761 (GRCm39)	R536G	possibly damaging	Het
Bltp1	A	T	3: 37,000,432 (GRCm39)	N1204Y	probably benign	Het
Catsperg1	T	C	7: 28,894,851 (GRCm39)	E582G	probably benign	Het
Ccdc136	A	G	6: 29,419,306 (GRCm39)	N942S	probably damaging	Het
Ccdc81	A	C	7: 89,524,988 (GRCm39)	M531R	possibly damaging	Het
Cep78	T	C	19: 15,953,672 (GRCm39)	E283G	probably benign	Het
Cnot1	ACG	A	8: 96,472,275 (GRCm39)		probably null	Het
E230025N22Rik	C	A	18: 36,828,605 (GRCm39)	R24S	possibly damaging	Het
Epb41l1	T	A	2: 156,364,128 (GRCm39)	M879K	probably benign	Het
Fam135a	T	C	1: 24,065,760 (GRCm39)	Y1226C	probably damaging	Het
Fbxo11	T	C	17: 88,320,031 (GRCm39)	E151G		Het
Herc6	A	G	6: 57,584,106 (GRCm39)	T322A	probably benign	Het
Inka1	T	C	9: 107,862,761 (GRCm39)		probably benign	Het
Kalrn	T	A	16: 33,849,122 (GRCm39)	Q2110L	probably benign	Het
Kti12	A	C	4: 108,705,443 (GRCm39)	E119A	probably benign	Het
Kti12	G	T	4: 108,705,444 (GRCm39)	E119D	probably benign	Het
Lrrc19	T	A	4: 94,526,537 (GRCm39)	H340L	probably damaging	Het
Mkks	A	T	2: 136,722,876 (GRCm39)	F94I	probably damaging	Het
Pcdhga3	T	A	18: 37,808,013 (GRCm39)	F155L	probably benign	Het
Pdlim1	T	A	19: 40,240,445 (GRCm39)	I53F	probably damaging	Het
Pttg1	A	T	11: 43,316,421 (GRCm39)	L26Q	probably benign	Het
Rasa4	T	A	5: 136,130,510 (GRCm39)		probably benign	Het
Rsbn1l	A	G	5: 21,110,896 (GRCm39)	S481P	probably damaging	Het
Sae1	C	A	7: 16,121,648 (GRCm39)	G10C	unknown	Het
Samd15	T	C	12: 87,248,559 (GRCm39)	S415P	probably damaging	Het
Scn7a	T	A	2: 66,530,294 (GRCm39)	I684F	probably damaging	Het
Sec23b	T	A	2: 144,406,565 (GRCm39)	M120K	probably benign	Het
Slc20a1	G	A	2: 129,049,757 (GRCm39)	D340N	probably benign	Het
Slf2	T	A	19: 44,947,499 (GRCm39)	M821K	possibly damaging	Het
Suv39h2	G	A	2: 3,465,453 (GRCm39)	R301*	probably null	Het
Tgfbi	A	G	13: 56,777,502 (GRCm39)	T329A	probably damaging	Het
Tmprss12	T	C	15: 100,183,111 (GRCm39)	F151S	probably damaging	Het
Ttn	T	C	2: 76,747,525 (GRCm39)	D4508G	probably benign	Het
Tyk2	C	A	9: 21,032,851 (GRCm39)	C304F	probably benign	Het
Vmn1r121	T	A	7: 20,831,589 (GRCm39)	T284S	probably benign	Het
Vmn2r117	A	G	17: 23,679,100 (GRCm39)	I708T	possibly damaging	Het
Zfc3h1	A	G	10: 115,239,062 (GRCm39)		probably null	Het
Zic4	T	G	9: 91,266,181 (GRCm39)	V275G	probably damaging	Het

Other mutations in Vmn2r2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00326:Vmn2r2	APN	3	64,041,319 (GRCm39)	splice site	probably benign
IGL00980:Vmn2r2	APN	3	64,024,601 (GRCm39)	missense	probably benign	0.11
IGL01389:Vmn2r2	APN	3	64,024,430 (GRCm39)	missense	probably damaging	0.97
IGL01804:Vmn2r2	APN	3	64,041,677 (GRCm39)	missense	possibly damaging	0.91
IGL02750:Vmn2r2	APN	3	64,024,823 (GRCm39)	missense	probably damaging	1.00
IGL02829:Vmn2r2	APN	3	64,026,172 (GRCm39)	splice site	probably benign
IGL03036:Vmn2r2	APN	3	64,024,321 (GRCm39)	missense	probably benign	0.01
IGL03174:Vmn2r2	APN	3	64,024,544 (GRCm39)	nonsense	probably null
PIT4151001:Vmn2r2	UTSW	3	64,024,334 (GRCm39)	missense	possibly damaging	0.67
R0029:Vmn2r2	UTSW	3	64,024,365 (GRCm39)	missense	probably benign	0.00
R0310:Vmn2r2	UTSW	3	64,042,039 (GRCm39)	missense	probably damaging	1.00
R0357:Vmn2r2	UTSW	3	64,041,320 (GRCm39)	splice site	probably null
R0637:Vmn2r2	UTSW	3	64,033,999 (GRCm39)	missense	probably benign	0.00
R1626:Vmn2r2	UTSW	3	64,041,921 (GRCm39)	missense	possibly damaging	0.61
R1662:Vmn2r2	UTSW	3	64,024,551 (GRCm39)	missense	probably benign	0.00
R1710:Vmn2r2	UTSW	3	64,024,820 (GRCm39)	missense	probably benign	0.19
R1797:Vmn2r2	UTSW	3	64,042,128 (GRCm39)	missense	probably benign	0.00
R1862:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
R1971:Vmn2r2	UTSW	3	64,034,121 (GRCm39)	missense	probably damaging	1.00
R2051:Vmn2r2	UTSW	3	64,024,766 (GRCm39)	missense	possibly damaging	0.77
R2099:Vmn2r2	UTSW	3	64,024,474 (GRCm39)	missense	probably damaging	1.00
R2275:Vmn2r2	UTSW	3	64,023,930 (GRCm39)	missense	probably benign	0.00
R3078:Vmn2r2	UTSW	3	64,042,053 (GRCm39)	missense	probably benign	0.22
R3418:Vmn2r2	UTSW	3	64,024,320 (GRCm39)	missense	probably benign	0.00
R3419:Vmn2r2	UTSW	3	64,024,320 (GRCm39)	missense	probably benign	0.00
R3959:Vmn2r2	UTSW	3	64,047,947 (GRCm39)	missense	probably benign
R4230:Vmn2r2	UTSW	3	64,041,912 (GRCm39)	missense	probably benign	0.00
R4258:Vmn2r2	UTSW	3	64,042,118 (GRCm39)	missense	probably damaging	1.00
R4810:Vmn2r2	UTSW	3	64,044,883 (GRCm39)	missense	probably damaging	1.00
R4822:Vmn2r2	UTSW	3	64,041,960 (GRCm39)	missense	probably damaging	0.99
R4919:Vmn2r2	UTSW	3	64,024,578 (GRCm39)	missense	possibly damaging	0.56
R4925:Vmn2r2	UTSW	3	64,044,892 (GRCm39)	start codon destroyed	probably null	0.03
R4954:Vmn2r2	UTSW	3	64,047,905 (GRCm39)	missense	probably benign	0.00
R5071:Vmn2r2	UTSW	3	64,024,321 (GRCm39)	missense	probably benign	0.01
R5315:Vmn2r2	UTSW	3	64,024,377 (GRCm39)	missense	probably benign	0.37
R5450:Vmn2r2	UTSW	3	64,034,011 (GRCm39)	missense	probably benign
R5577:Vmn2r2	UTSW	3	64,024,416 (GRCm39)	missense	probably benign	0.00
R5595:Vmn2r2	UTSW	3	64,034,036 (GRCm39)	missense	possibly damaging	0.74
R5727:Vmn2r2	UTSW	3	64,024,608 (GRCm39)	missense	probably benign	0.41
R5810:Vmn2r2	UTSW	3	64,024,815 (GRCm39)	missense	probably damaging	1.00
R5919:Vmn2r2	UTSW	3	64,044,723 (GRCm39)	missense	probably benign	0.39
R6052:Vmn2r2	UTSW	3	64,024,782 (GRCm39)	missense	possibly damaging	0.56
R6084:Vmn2r2	UTSW	3	64,024,467 (GRCm39)	missense	probably benign	0.01
R6299:Vmn2r2	UTSW	3	64,024,074 (GRCm39)	nonsense	probably null
R6762:Vmn2r2	UTSW	3	64,041,870 (GRCm39)	missense	probably damaging	1.00
R6858:Vmn2r2	UTSW	3	64,044,915 (GRCm39)	missense	probably damaging	1.00
R6889:Vmn2r2	UTSW	3	64,024,688 (GRCm39)	missense	probably damaging	0.99
R6990:Vmn2r2	UTSW	3	64,024,608 (GRCm39)	missense	probably benign	0.02
R7195:Vmn2r2	UTSW	3	64,023,900 (GRCm39)	missense	probably benign	0.01
R7269:Vmn2r2	UTSW	3	64,033,998 (GRCm39)	missense	probably benign	0.32
R7699:Vmn2r2	UTSW	3	64,024,536 (GRCm39)	missense	possibly damaging	0.69
R7717:Vmn2r2	UTSW	3	64,042,019 (GRCm39)	missense	possibly damaging	0.95
R7798:Vmn2r2	UTSW	3	64,041,518 (GRCm39)	missense	possibly damaging	0.60
R7974:Vmn2r2	UTSW	3	64,024,808 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r2	UTSW	3	64,044,858 (GRCm39)	missense	probably damaging	1.00
R8478:Vmn2r2	UTSW	3	64,024,257 (GRCm39)	missense	possibly damaging	0.87
R8731:Vmn2r2	UTSW	3	64,024,404 (GRCm39)	missense	probably benign	0.16
R9035:Vmn2r2	UTSW	3	64,024,172 (GRCm39)	missense	probably damaging	1.00
R9182:Vmn2r2	UTSW	3	64,044,802 (GRCm39)	missense	probably damaging	1.00
R9225:Vmn2r2	UTSW	3	64,034,021 (GRCm39)	missense	probably benign	0.00
R9266:Vmn2r2	UTSW	3	64,024,057 (GRCm39)	missense	probably damaging	0.98
R9427:Vmn2r2	UTSW	3	64,041,945 (GRCm39)	missense	probably damaging	1.00
R9659:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
R9666:Vmn2r2	UTSW	3	64,023,870 (GRCm39)	missense	probably benign	0.08
R9771:Vmn2r2	UTSW	3	64,042,079 (GRCm39)	missense	possibly damaging	0.88
R9788:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
X0024:Vmn2r2	UTSW	3	64,044,707 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GAACATGCTGTATCATTCCTCTG -3'
(R):5'- TGCAAAGCCTGAGTATTTCCCC -3'

Sequencing Primer
(F):5'- CTGTTCTTCCAATAAGTCACAAGTGC -3'
(R):5'- GGTGGAACTATTGGATTTGCAATACC -3'

Posted On

2020-09-15