Mutagenetix > Incidental Mutation

Incidental Mutation 'R7920:Zkscan7'

648298

Institutional Source

Beutler Lab

Gene Symbol

Zkscan7

Ensembl Gene

ENSMUSG00000063488

Gene Name

zinc finger with KRAB and SCAN domains 7

Synonyms

Zfp167

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7920 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

122885685-122898618 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 122895909 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 648 (T648S)

Ref Sequence

ENSEMBL: ENSMUSP00000071695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000063980] [ENSMUST00000215872]

AlphaFold

E9PVW1

Predicted Effect

probably benign
Transcript: ENSMUST00000063980
AA Change: T648S

PolyPhen 2 Score 0.188 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000071695
Gene: ENSMUSG00000063488
AA Change: T648S

Domain	Start	End	E-Value	Type
SCAN	45	156	1.18e-65	SMART
ZnF_C2H2	350	372	5.59e-4	SMART
ZnF_C2H2	378	400	3.69e-4	SMART
ZnF_C2H2	434	456	8.4e1	SMART
ZnF_C2H2	487	509	4.24e-4	SMART
ZnF_C2H2	515	537	8.34e-3	SMART
ZnF_C2H2	543	565	7.37e-4	SMART
ZnF_C2H2	571	593	1.92e-2	SMART
ZnF_C2H2	599	621	1.13e-4	SMART
ZnF_C2H2	627	649	2.24e-3	SMART
ZnF_C2H2	655	677	4.17e-3	SMART
ZnF_C2H2	683	705	1.04e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000215872

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700024B05Rik	T	C	14: 41,996,173	S9P	probably damaging	Het
AI661453	A	T	17: 47,468,406	Q1019L	unknown	Het
Aldh1a1	T	A	19: 20,617,937	W77R	probably damaging	Het
Cc2d2a	T	C	5: 43,739,309	I1516T	probably benign	Het
Ccdc149	T	A	5: 52,405,094	I197F	probably damaging	Het
Ccdc189	A	G	7: 127,587,953	M46T	probably benign	Het
Cfb	A	G	17: 34,860,891	V176A	probably benign	Het
Chordc1	A	G	9: 18,302,101	K83E	probably benign	Het
Cic	G	A	7: 25,271,959	V372M	probably benign	Het
Coq2	T	A	5: 100,663,875		probably benign	Het
Cradd	A	G	10: 95,322,711	L58P	probably damaging	Het
Crim1	A	T	17: 78,303,064	D316V	probably damaging	Het
Cyb5rl	C	A	4: 107,071,008	L114I	possibly damaging	Het
Cyp2c69	T	G	19: 39,877,803		probably null	Het
Ddhd1	A	G	14: 45,657,470	F181S	probably damaging	Het
Dennd1b	G	A	1: 139,062,873	E192K	probably damaging	Het
Dmrt1	T	C	19: 25,506,019	S56P	possibly damaging	Het
Dnah12	A	G	14: 26,856,542	E3086G	possibly damaging	Het
Dnah5	A	T	15: 28,453,222	M4380L	probably benign	Het
Dph7	A	T	2: 24,971,612	M346L	probably benign	Het
Dusp27	T	A	1: 166,099,896	N716Y	possibly damaging	Het
Egf	C	T	3: 129,735,840	R307H	probably benign	Het
Fam50b	G	A	13: 34,747,101	E187K	possibly damaging	Het
Fcna	T	C	2: 25,626,286	K112E	probably benign	Het
Fkbp5	A	T	17: 28,429,239	N125K	possibly damaging	Het
Foxo3	C	T	10: 42,197,769	V251I	possibly damaging	Het
Fryl	T	A	5: 73,101,807		probably null	Het
Fsip2	A	G	2: 82,951,021	E253G	possibly damaging	Het
Gm49333	A	G	16: 20,637,692	E428G	possibly damaging	Het
Gprc6a	T	A	10: 51,614,930	T908S	probably benign	Het
Grin2c	A	T	11: 115,254,144	F560L	probably benign	Het
Hao1	T	A	2: 134,548,252	N56Y	probably damaging	Het
Haus3	T	C	5: 34,167,702	I204M	probably benign	Het
Htr3b	C	A	9: 48,937,156	C263F	probably damaging	Het
Ica1	A	T	6: 8,742,274	C120S	probably benign	Het
Inpp4a	T	A	1: 37,367,805	S210T	probably benign	Het
Inpp5d	T	G	1: 87,705,949	I699S	probably damaging	Het
Kcng1	A	G	2: 168,262,984	V314A	probably benign	Het
Lcn11	G	A	2: 25,779,331	V167M	possibly damaging	Het
Lingo3	C	A	10: 80,834,548	R516L	probably benign	Het
Lrmda	T	C	14: 22,596,478	V151A	probably damaging	Het
Ly6g5b	A	C	17: 35,114,602	I133R	probably damaging	Het
Mcidas	G	A	13: 112,998,987	G315S	probably damaging	Het
Miip	C	T	4: 147,862,918	G236S	probably benign	Het
Mroh7	T	C	4: 106,707,576	T562A	probably benign	Het
Nlrc4	A	G	17: 74,427,119	M933T	probably benign	Het
Olfr539	A	T	7: 140,667,901	S198C	possibly damaging	Het
Pkhd1	A	T	1: 20,275,535	D2756E	probably damaging	Het
Plekhm2	T	C	4: 141,632,121	D445G	probably damaging	Het
Ppp1r12c	C	T	7: 4,483,355	G605D	probably benign	Het
Pygb	T	A	2: 150,787,002	N45K	possibly damaging	Het
Scn4b	A	G	9: 45,146,771	T54A	probably damaging	Het
Scrt1	T	A	15: 76,519,217	H191L	unknown	Het
Sdc3	A	G	4: 130,819,196	M289V	probably benign	Het
Shb	C	T	4: 45,489,054		probably null	Het
Slc1a5	A	G	7: 16,793,870	T364A	probably damaging	Het
Slc25a27	A	G	17: 43,649,673	V218A	probably benign	Het
Slc39a4	C	T	15: 76,614,085	G384D	probably damaging	Het
Sptbn2	C	G	19: 4,749,012	R2037G	probably benign	Het
Tmem67	A	T	4: 12,089,284		probably null	Het
Tonsl	C	T	15: 76,634,587	R544H	probably damaging	Het
Trex1	A	G	9: 109,058,089	V278A	unknown	Het
Ttn	A	T	2: 76,795,157	D15107E	probably damaging	Het
Tubgcp5	A	G	7: 55,816,562	T621A	probably benign	Het
Zfhx4	T	C	3: 5,400,455	V1916A	possibly damaging	Het
Zfp260	A	T	7: 30,105,592	K306*	probably null	Het
Zscan4c	T	A	7: 11,009,772	F433I	possibly damaging	Het

Other mutations in Zkscan7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00594:Zkscan7	APN	9	122895594	missense	possibly damaging	0.95
IGL01650:Zkscan7	APN	9	122894827	missense	probably benign
IGL01905:Zkscan7	APN	9	122890761	missense	possibly damaging	0.93
IGL02466:Zkscan7	APN	9	122888885	missense	probably damaging	0.98
R0310:Zkscan7	UTSW	9	122888893	nonsense	probably null
R0355:Zkscan7	UTSW	9	122888807	missense	probably damaging	1.00
R0477:Zkscan7	UTSW	9	122890809	splice site	probably null
R1276:Zkscan7	UTSW	9	122890723	missense	probably damaging	0.98
R1426:Zkscan7	UTSW	9	122895163	missense	probably benign
R2055:Zkscan7	UTSW	9	122888937	missense	probably damaging	1.00
R2195:Zkscan7	UTSW	9	122895621	missense	possibly damaging	0.73
R2354:Zkscan7	UTSW	9	122894827	missense	probably benign
R4878:Zkscan7	UTSW	9	122890800	nonsense	probably null
R5106:Zkscan7	UTSW	9	122896133	unclassified	probably benign
R6266:Zkscan7	UTSW	9	122895234	nonsense	probably null
R6299:Zkscan7	UTSW	9	122888717	missense	probably damaging	1.00
R6513:Zkscan7	UTSW	9	122896105	missense	probably benign	0.00
R6881:Zkscan7	UTSW	9	122888701	missense	possibly damaging	0.96
R7640:Zkscan7	UTSW	9	122896056	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- CTGGCGAGGCATTCATTTGG -3'
(R):5'- CGCGACATTGTGAATTCGCTG -3'

Sequencing Primer
(F):5'- GTGTGGGAAAGCCTTCTATACCAAC -3'
(R):5'- AATTCGCTGGTGGTGATTAAAG -3'

Posted On

2020-09-15