Mutagenetix > Incidental Mutation

Incidental Mutation 'R7934:Zfp715'

648618

Institutional Source

Beutler Lab

Gene Symbol

Zfp715

Ensembl Gene

ENSMUSG00000012640

Gene Name

zinc finger protein 715

Synonyms

2610041B18Rik, mszf15

MMRRC Submission

045980-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R7934 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

42945946-42962724 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 42949308 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 217 (Y217*)

Ref Sequence

ENSEMBL: ENSMUSP00000040282 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000012796] [ENSMUST00000048015] [ENSMUST00000107986] [ENSMUST00000135130] [ENSMUST00000139061] [ENSMUST00000145622]

AlphaFold

G3X9T1

Predicted Effect

probably benign
Transcript: ENSMUST00000012796

SMART Domains

Protein: ENSMUSP00000012796
Gene: ENSMUSG00000012640

Domain	Start	End	E-Value	Type
KRAB	48	108	3.87e-30	SMART
ZnF_C2H2	124	146	3.39e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000048015
AA Change: Y217*

SMART Domains

Protein: ENSMUSP00000040282
Gene: ENSMUSG00000012640
AA Change: Y217*

Domain	Start	End	E-Value	Type
KRAB	48	108	3.87e-30	SMART
ZnF_C2H2	356	378	1.28e-3	SMART
ZnF_C2H2	384	406	1.03e-2	SMART
ZnF_C2H2	412	434	4.79e-3	SMART
ZnF_C2H2	440	462	1.45e-2	SMART
ZnF_C2H2	468	490	1.12e-3	SMART
ZnF_C2H2	496	518	2.57e-3	SMART
ZnF_C2H2	524	546	4.79e-3	SMART
ZnF_C2H2	552	574	1.92e-2	SMART
ZnF_C2H2	580	602	9.58e-3	SMART
ZnF_C2H2	608	630	8.6e-5	SMART
ZnF_C2H2	636	658	1.13e-4	SMART
ZnF_C2H2	691	713	1.98e-4	SMART
ZnF_C2H2	719	741	8.34e-3	SMART
ZnF_C2H2	747	769	3.95e-4	SMART
ZnF_C2H2	775	797	4.94e-5	SMART
ZnF_C2H2	803	825	3.39e-3	SMART
ZnF_C2H2	831	853	1.95e-3	SMART
ZnF_C2H2	859	881	2.09e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000107986
AA Change: Y217*

SMART Domains

Protein: ENSMUSP00000103620
Gene: ENSMUSG00000012640
AA Change: Y217*

Domain	Start	End	E-Value	Type
KRAB	48	108	3.87e-30	SMART
ZnF_C2H2	356	378	1.28e-3	SMART
ZnF_C2H2	384	406	1.03e-2	SMART
ZnF_C2H2	412	434	4.79e-3	SMART
ZnF_C2H2	440	462	1.45e-2	SMART
ZnF_C2H2	468	490	1.12e-3	SMART
ZnF_C2H2	496	518	2.57e-3	SMART
ZnF_C2H2	524	546	4.79e-3	SMART
ZnF_C2H2	552	574	1.92e-2	SMART
ZnF_C2H2	580	602	9.58e-3	SMART
ZnF_C2H2	608	630	8.6e-5	SMART
ZnF_C2H2	636	658	1.13e-4	SMART
ZnF_C2H2	691	713	1.98e-4	SMART
ZnF_C2H2	719	741	8.34e-3	SMART
ZnF_C2H2	747	769	3.95e-4	SMART
ZnF_C2H2	775	797	4.94e-5	SMART
ZnF_C2H2	803	825	3.39e-3	SMART
ZnF_C2H2	831	853	1.95e-3	SMART
ZnF_C2H2	859	881	2.09e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135130

SMART Domains

Protein: ENSMUSP00000122812
Gene: ENSMUSG00000012640

Domain	Start	End	E-Value	Type
KRAB	48	88	5.49e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000139061

SMART Domains

Protein: ENSMUSP00000114600
Gene: ENSMUSG00000012640

Domain	Start	End	E-Value	Type
KRAB	48	108	3.87e-30	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000145622

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110082I17Rik	G	A	5: 139,349,770 (GRCm39)	A93V	probably benign	Het
Abca8b	G	A	11: 109,865,865 (GRCm39)	T296I	possibly damaging	Het
Adamtsl1	A	C	4: 86,161,962 (GRCm39)	Y365S	probably damaging	Het
Ambp	T	A	4: 63,067,677 (GRCm39)	I178F	probably damaging	Het
Ankle1	T	C	8: 71,858,899 (GRCm39)	V44A	possibly damaging	Het
Arhgef2	A	G	3: 88,537,221 (GRCm39)	E37G	probably damaging	Het
Atp2a2	A	G	5: 122,599,639 (GRCm39)	I584T	probably benign	Het
Ccdc171	T	A	4: 83,614,492 (GRCm39)	L1031*	probably null	Het
Cdh17	T	A	4: 11,799,754 (GRCm39)		probably null	Het
Cdip1	T	C	16: 4,586,422 (GRCm39)	T203A	probably benign	Het
Ceacam1	C	T	7: 25,163,220 (GRCm39)	V338I	possibly damaging	Het
Cep78	G	A	19: 15,933,754 (GRCm39)	T608I	probably damaging	Het
Ces3a	T	A	8: 105,775,345 (GRCm39)		probably null	Het
Cfap210	G	A	2: 69,612,450 (GRCm39)	Q97*	probably null	Het
Chd7	A	G	4: 8,854,121 (GRCm39)	T1898A	probably benign	Het
Cpne6	T	C	14: 55,750,066 (GRCm39)	C66R	possibly damaging	Het
Creb1	G	A	1: 64,609,372 (GRCm39)	A108T	probably benign	Het
Ctnna3	A	T	10: 64,421,747 (GRCm39)	I514F	probably damaging	Het
Cyp2c55	T	C	19: 39,030,535 (GRCm39)	F456L	probably damaging	Het
Cyp2d40	A	G	15: 82,648,212 (GRCm39)	S34P	probably damaging	Het
Cyp2g1	A	T	7: 26,518,618 (GRCm39)	T378S	probably damaging	Het
Eif2b3	C	A	4: 116,923,675 (GRCm39)	Q297K	probably benign	Het
Fbll1	T	C	11: 35,689,048 (GRCm39)	N72D	unknown	Het
Fev	C	G	1: 74,921,632 (GRCm39)	R106P	probably damaging	Het
Gal3st1	A	G	11: 3,948,405 (GRCm39)	Y204C	probably damaging	Het
Gm15130	A	T	2: 110,964,582 (GRCm39)	L180Q		Het
Greb1l	A	T	18: 10,474,371 (GRCm39)	K229*	probably null	Het
Haghl	G	T	17: 26,002,519 (GRCm39)	T214K	probably damaging	Het
Hivep1	A	G	13: 42,308,174 (GRCm39)	H138R	probably benign	Het
Ighv1-53	G	T	12: 115,122,236 (GRCm39)	Y46*	probably null	Het
Igsf21	T	C	4: 139,761,755 (GRCm39)	T308A	possibly damaging	Het
Kcnb1	A	G	2: 166,946,536 (GRCm39)	S771P	probably benign	Het
Kndc1	T	A	7: 139,501,402 (GRCm39)	M897K	probably benign	Het
Macf1	T	C	4: 123,367,727 (GRCm39)	M2345V	possibly damaging	Het
Mettl2	T	C	11: 105,019,773 (GRCm39)	S137P	probably benign	Het
Mrpl15	A	G	1: 4,844,725 (GRCm39)	S189P	probably benign	Het
Ndrg2	T	C	14: 52,143,661 (GRCm39)	Q351R	probably benign	Het
Nox4	A	G	7: 86,945,032 (GRCm39)	N65S	probably damaging	Het
Or1n1	A	G	2: 36,750,228 (GRCm39)	I44T	possibly damaging	Het
Or1p1b	C	T	11: 74,131,326 (GRCm39)	S312F	probably benign	Het
Osbpl11	T	A	16: 33,056,752 (GRCm39)	W729R	probably damaging	Het
Oxct2b	C	A	4: 123,010,447 (GRCm39)	C122*	probably null	Het
Pdcl3	A	T	1: 39,026,782 (GRCm39)		probably benign	Het
Plcg1	A	G	2: 160,616,498 (GRCm39)	H1294R	possibly damaging	Het
Pnp2	C	A	14: 51,201,903 (GRCm39)	S296R	probably benign	Het
Ppp1r12c	G	A	7: 4,488,416 (GRCm39)	Q337*	probably null	Het
Prr23a4	A	G	9: 98,785,880 (GRCm39)	R182G	possibly damaging	Het
Ranbp2	G	A	10: 58,312,297 (GRCm39)	V1006I	probably damaging	Het
Rxrg	A	G	1: 167,454,927 (GRCm39)	D170G	probably damaging	Het
Sf3b3	T	C	8: 111,548,162 (GRCm39)	T691A	probably benign	Het
Slc1a4	A	G	11: 20,258,518 (GRCm39)	I307T	probably damaging	Het
Slc4a8	G	A	15: 100,685,173 (GRCm39)	V235I	probably damaging	Het
Srgap3	T	A	6: 112,708,450 (GRCm39)	I750F	probably damaging	Het
Trim30a	A	T	7: 104,061,448 (GRCm39)	L282Q	probably damaging	Het
Tshr	A	G	12: 91,478,702 (GRCm39)	K183R	possibly damaging	Het
Usp38	A	T	8: 81,711,077 (GRCm39)	L986Q	probably damaging	Het
Vmn1r257	T	A	7: 22,391,439 (GRCm39)	T102S	possibly damaging	Het
Vps18	A	G	2: 119,124,122 (GRCm39)	T350A	probably benign	Het
Zan	T	A	5: 137,461,841 (GRCm39)	T1113S	unknown	Het
Zbtb37	A	T	1: 160,857,305 (GRCm39)	S312R	probably damaging	Het
Zeb1	A	G	18: 5,748,703 (GRCm39)	N90S	probably benign	Het
Zfp9	T	C	6: 118,441,847 (GRCm39)	T272A	probably damaging	Het
Zfp943	T	A	17: 22,212,361 (GRCm39)	H482Q	probably damaging	Het
Zfp971	A	T	2: 177,675,173 (GRCm39)	K257N	probably benign	Het

Other mutations in Zfp715

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00093:Zfp715	APN	7	42,949,173 (GRCm39)	missense	possibly damaging	0.53
IGL00984:Zfp715	APN	7	42,949,208 (GRCm39)	missense	probably benign	0.28
IGL03401:Zfp715	APN	7	42,949,160 (GRCm39)	missense	probably benign	0.18
R0373:Zfp715	UTSW	7	42,948,760 (GRCm39)	missense	possibly damaging	0.96
R1167:Zfp715	UTSW	7	42,947,861 (GRCm39)	missense	possibly damaging	0.83
R1943:Zfp715	UTSW	7	42,949,054 (GRCm39)	missense	possibly damaging	0.86
R1987:Zfp715	UTSW	7	42,948,073 (GRCm39)	missense	possibly damaging	0.71
R2073:Zfp715	UTSW	7	42,960,544 (GRCm39)	missense	probably benign	0.01
R2116:Zfp715	UTSW	7	42,947,370 (GRCm39)	missense	possibly damaging	0.71
R2403:Zfp715	UTSW	7	42,948,692 (GRCm39)	missense	possibly damaging	0.91
R3707:Zfp715	UTSW	7	42,960,553 (GRCm39)	missense	probably benign
R3838:Zfp715	UTSW	7	42,949,180 (GRCm39)	missense	probably benign	0.33
R4059:Zfp715	UTSW	7	42,951,155 (GRCm39)	missense	probably benign	0.11
R4110:Zfp715	UTSW	7	42,947,304 (GRCm39)	missense	possibly damaging	0.72
R4426:Zfp715	UTSW	7	42,960,516 (GRCm39)	missense	probably damaging	0.99
R4675:Zfp715	UTSW	7	42,949,444 (GRCm39)	missense	probably benign	0.15
R4898:Zfp715	UTSW	7	42,949,106 (GRCm39)	missense	possibly damaging	0.48
R5007:Zfp715	UTSW	7	42,949,019 (GRCm39)	missense	possibly damaging	0.53
R5477:Zfp715	UTSW	7	42,949,378 (GRCm39)	missense	probably damaging	0.99
R5574:Zfp715	UTSW	7	42,960,463 (GRCm39)	missense	possibly damaging	0.94
R5594:Zfp715	UTSW	7	42,949,116 (GRCm39)	missense	possibly damaging	0.73
R5967:Zfp715	UTSW	7	42,948,572 (GRCm39)	missense	probably benign	0.04
R6538:Zfp715	UTSW	7	42,948,573 (GRCm39)	missense	possibly damaging	0.73
R7322:Zfp715	UTSW	7	42,960,562 (GRCm39)	missense	possibly damaging	0.53
R7629:Zfp715	UTSW	7	42,951,100 (GRCm39)	missense	possibly damaging	0.92
R7973:Zfp715	UTSW	7	42,949,321 (GRCm39)	missense	possibly damaging	0.64
R8327:Zfp715	UTSW	7	42,947,482 (GRCm39)	missense	possibly damaging	0.64
R8348:Zfp715	UTSW	7	42,949,361 (GRCm39)	missense	possibly damaging	0.53
R8532:Zfp715	UTSW	7	42,949,134 (GRCm39)	missense	probably benign	0.00
R8532:Zfp715	UTSW	7	42,948,829 (GRCm39)	missense	possibly damaging	0.71
R8835:Zfp715	UTSW	7	42,948,430 (GRCm39)	missense
R9150:Zfp715	UTSW	7	42,948,713 (GRCm39)	missense	possibly damaging	0.85
R9328:Zfp715	UTSW	7	42,947,328 (GRCm39)	missense	possibly damaging	0.71
R9332:Zfp715	UTSW	7	42,948,847 (GRCm39)	missense	probably damaging	0.98
R9619:Zfp715	UTSW	7	42,949,104 (GRCm39)	nonsense	probably null
R9649:Zfp715	UTSW	7	42,950,653 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGAAAACGGTTTCCATAGATCAGTG -3'
(R):5'- AACTATGGCAGGGTGCTGAC -3'

Sequencing Primer
(F):5'- ATGGCTGAGAACATCTCCATTC -3'
(R):5'- GGCAGGGTGCTGACATTAC -3'

Posted On

2020-09-15