Mutagenetix > Incidental Mutation

Incidental Mutation 'R7943:Olfr585'

649157

Institutional Source

Beutler Lab

Gene Symbol

Olfr585

Ensembl Gene

ENSMUSG00000078080

Gene Name

olfactory receptor 585

Synonyms

MOR14-4, GA_x6K02T2PBJ9-5809085-5810035

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R7943 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

103097720-103098749 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 103097946 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 68 (M68I)

Ref Sequence

ENSEMBL: ENSMUSP00000100476 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000104881]

AlphaFold

E9PXW4

Predicted Effect

probably damaging
Transcript: ENSMUST00000104881
AA Change: M68I

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000100476
Gene: ENSMUSG00000078080
AA Change: M68I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	40	318	1.3e-109	PFAM
Pfam:7TM_GPCR_Srsx	45	315	5.9e-6	PFAM
Pfam:7tm_1	50	300	7.9e-23	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (68/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1a	C	T	5: 8,686,222	T205I	probably benign	Het
Acsbg1	T	C	9: 54,622,737	H225R	probably damaging	Het
Anks1	T	A	17: 27,985,204	Y209N	probably damaging	Het
Appl1	T	A	14: 26,945,568	I377L	probably benign	Het
Arl9	A	T	5: 77,010,548	D159V	probably damaging	Het
Arsa	A	C	15: 89,474,089	L339R	probably damaging	Het
C2	A	G	17: 34,872,378	L380P	probably damaging	Het
Ccdc13	A	G	9: 121,799,130	C97R	unknown	Het
Ccdc74a	A	G	16: 17,650,552	H346R	probably benign	Het
Ccnl1	T	C	3: 65,956,905	I152V	probably benign	Het
Cd4	C	T	6: 124,870,244		probably null	Het
Ceacam5	A	T	7: 17,745,566	I203L	probably benign	Het
Ckap2	C	A	8: 22,175,074	R458L	probably damaging	Het
Cldn10	T	C	14: 118,861,859		probably null	Het
Col27a1	C	T	4: 63,318,283	R1377C	unknown	Het
Cry1	A	T	10: 85,143,120	M514K	probably benign	Het
Crybg2	T	G	4: 134,072,984	V176G	probably damaging	Het
Cyp2j5	C	T	4: 96,659,612	G131D	possibly damaging	Het
Ddx46	A	G	13: 55,669,722	Y720C	probably damaging	Het
Dock10	A	T	1: 80,648,289	V44D	probably damaging	Het
Enam	A	T	5: 88,488,551		probably null	Het
Fam184a	C	A	10: 53,633,706	E126*	probably null	Het
Fam184a	C	T	10: 53,647,041	A956T	probably damaging	Het
Fbxw10	A	T	11: 62,850,661	R202*	probably null	Het
Fnip1	A	G	11: 54,502,388	E550G	probably damaging	Het
Gm37596	A	T	3: 93,692,763	C100S	probably benign	Het
Gm8300	C	T	12: 87,517,003	A36V	probably damaging	Het
Gpr149	A	G	3: 62,530,711	L675P	probably damaging	Het
Hivep3	T	C	4: 120,132,357	Y2002H	probably benign	Het
Hp	A	G	8: 109,575,555	Y254H	probably damaging	Het
Ighg1	G	T	12: 113,330,337	T62N		Het
Jcad	A	G	18: 4,672,700	E154G	probably damaging	Het
Kmt2a	A	G	9: 44,849,140	S471P	probably damaging	Het
Med13	A	G	11: 86,278,526	V1968A	probably damaging	Het
Mknk2	T	A	10: 80,675,867	Q3L	probably benign	Het
Nup98	G	A	7: 102,194,822	T65I	probably benign	Het
Olfr1028	A	G	2: 85,951,998	T312A	probably benign	Het
Olfr1499	A	T	19: 13,815,236	M118K	probably damaging	Het
Olfr763	T	A	10: 129,012,065	M260K	possibly damaging	Het
Pcdhgb4	C	T	18: 37,722,010	T486I	probably benign	Het
Perm1	T	A	4: 156,218,534	F512I	probably damaging	Het
Pgap3	A	T	11: 98,390,401	L262Q	probably damaging	Het
Pklr	A	C	3: 89,141,507	Y126S	probably damaging	Het
Ppl	C	T	16: 5,088,861	R1190H	probably damaging	Het
Ppm1k	T	C	6: 57,524,828	T117A	probably benign	Het
Prkd2	G	A	7: 16,850,319	E366K	probably benign	Het
Psd	C	A	19: 46,324,730	C67F	possibly damaging	Het
Ptpa	T	A	2: 30,432,044	F100L	probably damaging	Het
Rftn1	G	T	17: 50,047,380	A318D	probably damaging	Het
Rock1	T	C	18: 10,112,357	E466G	probably damaging	Het
Sec16b	A	T	1: 157,554,757	M588L	probably benign	Het
Serpina1f	T	A	12: 103,693,690	H111L	probably damaging	Het
Sf3a1	T	C	11: 4,166,537	I76T	possibly damaging	Het
Shcbp1	A	T	8: 4,748,812	L369Q	possibly damaging	Het
Spef2	A	G	15: 9,601,085	M1697T	unknown	Het
St7	G	A	6: 17,844,912	C133Y	probably damaging	Het
Tex19.1	T	A	11: 121,147,160	W115R	possibly damaging	Het
Tfrc	T	A	16: 32,630,221	I726N	probably benign	Het
Thbs1	A	G	2: 118,119,617		probably null	Het
Trim43a	C	T	9: 88,582,185	P50S	probably benign	Het
Trpm4	A	T	7: 45,308,681	V935E	probably damaging	Het
Ttc12	A	T	9: 49,470,320	V117D	possibly damaging	Het
Ulbp1	T	C	10: 7,457,053	T82A	probably damaging	Het
Usp13	C	T	3: 32,876,940	H288Y	probably damaging	Het
Vmn2r82	A	G	10: 79,396,245	K693E	possibly damaging	Het
Vmn2r90	A	G	17: 17,712,304	T158A	probably damaging	Het
Vps8	A	G	16: 21,477,872	K540R	possibly damaging	Het
Zfp143	T	A	7: 110,072,474		probably null	Het
Zfp709	G	T	8: 71,890,089	C454F	probably damaging	Het

Other mutations in Olfr585

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01022:Olfr585	APN	7	103097870	missense	probably damaging	1.00
IGL02866:Olfr585	APN	7	103098383	missense	probably damaging	0.99
FR4548:Olfr585	UTSW	7	103098309	nonsense	probably null
FR4976:Olfr585	UTSW	7	103098309	small insertion	probably benign
R0893:Olfr585	UTSW	7	103098434	missense	probably benign	0.01
R0926:Olfr585	UTSW	7	103097885	missense	probably damaging	1.00
R1486:Olfr585	UTSW	7	103098430	missense	probably damaging	1.00
R2031:Olfr585	UTSW	7	103098164	missense	probably damaging	0.98
R3852:Olfr585	UTSW	7	103098184	missense	probably damaging	0.97
R4849:Olfr585	UTSW	7	103098319	missense	possibly damaging	0.95
R5241:Olfr585	UTSW	7	103098317	missense	probably benign	0.36
R5668:Olfr585	UTSW	7	103097896	missense	probably benign	0.42
R5841:Olfr585	UTSW	7	103097954	missense	probably damaging	1.00
R6902:Olfr585	UTSW	7	103098355	missense	probably benign	0.12
R8265:Olfr585	UTSW	7	103098097	missense	probably benign	0.00
R8969:Olfr585	UTSW	7	103098044	missense	probably damaging	0.99
R9345:Olfr585	UTSW	7	103098506	missense	possibly damaging	0.93
R9376:Olfr585	UTSW	7	103097764	missense	probably benign	0.01
R9702:Olfr585	UTSW	7	103098136	missense	probably damaging	0.99
RF003:Olfr585	UTSW	7	103098305
RF003:Olfr585	UTSW	7	103098306	nonsense	probably null
RF004:Olfr585	UTSW	7	103098305
RF004:Olfr585	UTSW	7	103098308	small insertion	probably benign
RF004:Olfr585	UTSW	7	103098309	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GCAAATGCTACAAATGCAGGAC -3'
(R):5'- TTGCAGATCGCTACATAACGATC -3'

Sequencing Primer
(F):5'- TGCAGGACAACACAGAATTCCTAAG -3'
(R):5'- GCAGATCGCTACATAACGATCAAAAG -3'

Posted On

2020-09-15