Incidental Mutation 'R7947:Arhgap25'
ID 649399
Institutional Source Beutler Lab
Gene Symbol Arhgap25
Ensembl Gene ENSMUSG00000030047
Gene Name Rho GTPase activating protein 25
Synonyms A130039I20Rik
MMRRC Submission 045992-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R7947 (G1)
Quality Score 225.009
Status Validated
Chromosome 6
Chromosomal Location 87435527-87510241 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 87440069 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 526 (D526G)
Ref Sequence ENSEMBL: ENSMUSP00000109267 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000071024] [ENSMUST00000101197] [ENSMUST00000113637]
AlphaFold Q8BYW1
Predicted Effect probably benign
Transcript: ENSMUST00000071024
AA Change: D437G

PolyPhen 2 Score 0.320 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000068964
Gene: ENSMUSG00000030047
AA Change: D437G

DomainStartEndE-ValueType
PDB:1V89|A 1 63 7e-33 PDB
Blast:RhoGAP 16 66 9e-22 BLAST
RhoGAP 86 262 6.28e-64 SMART
coiled coil region 454 552 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000101197
AA Change: D500G

PolyPhen 2 Score 0.320 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000098758
Gene: ENSMUSG00000030047
AA Change: D500G

DomainStartEndE-ValueType
PH 21 127 2.11e-21 SMART
RhoGAP 149 325 6.28e-64 SMART
coiled coil region 517 615 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000113637
AA Change: D526G

PolyPhen 2 Score 0.320 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000109267
Gene: ENSMUSG00000030047
AA Change: D526G

DomainStartEndE-ValueType
PH 47 153 2.11e-21 SMART
RhoGAP 175 351 6.28e-64 SMART
coiled coil region 543 641 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 98% (60/61)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit altered leukocyte transendothelial migration. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 70 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2310034C09Rik A G 16: 88,555,938 (GRCm39) T51A probably benign Het
Abcc8 A G 7: 45,754,886 (GRCm39) probably null Het
Accs A T 2: 93,674,602 (GRCm39) M123K probably damaging Het
Ace G A 11: 105,863,880 (GRCm39) G443S possibly damaging Het
Adprhl1 C T 8: 13,298,682 (GRCm39) V83I probably damaging Het
Arhgef18 T C 8: 3,482,775 (GRCm39) L160P probably damaging Het
Arnt T G 3: 95,381,837 (GRCm39) probably null Het
Art3 A G 5: 92,540,359 (GRCm39) N34S possibly damaging Het
Avl9 A T 6: 56,700,526 (GRCm39) E53D possibly damaging Het
AW209491 A T 13: 14,811,447 (GRCm39) E100V probably benign Het
Axin2 T C 11: 108,814,529 (GRCm39) I139T probably damaging Het
Birc7 G T 2: 180,575,103 (GRCm39) V280L probably damaging Het
Bmal1 T C 7: 112,886,353 (GRCm39) Y137H probably damaging Het
C1ra A G 6: 124,494,338 (GRCm39) N262D probably benign Het
Cacna1g A G 11: 94,348,001 (GRCm39) F696L probably benign Het
Casp14 T A 10: 78,550,079 (GRCm39) probably null Het
Ciao2a A G 9: 66,045,684 (GRCm39) I151V probably benign Het
Crlf1 T C 8: 70,951,862 (GRCm39) Y176H probably damaging Het
Dnajb4 A T 3: 151,892,468 (GRCm39) S122T probably benign Het
Dthd1 G A 5: 62,971,653 (GRCm39) D159N possibly damaging Het
Erich6 A T 3: 58,528,699 (GRCm39) I517N possibly damaging Het
Fam118b T A 9: 35,129,239 (GRCm39) H343L probably benign Het
Fat2 T A 11: 55,178,560 (GRCm39) D1500V probably damaging Het
Fcgbp T C 7: 27,803,595 (GRCm39) probably null Het
Fgfrl1 A G 5: 108,853,142 (GRCm39) D255G probably damaging Het
Gm9611 G A 14: 42,118,087 (GRCm39) Q82* probably null Het
Ifit3 G T 19: 34,565,359 (GRCm39) E302* probably null Het
Igkv3-9 G A 6: 70,565,737 (GRCm39) C112Y probably damaging Het
Ints4 A G 7: 97,148,792 (GRCm39) T233A probably benign Het
Khnyn A T 14: 56,125,059 (GRCm39) S438C probably damaging Het
Kifc1 T C 17: 34,102,849 (GRCm39) R192G probably benign Het
Kntc1 T C 5: 123,919,951 (GRCm39) S98P unknown Het
Krt1c A T 15: 101,724,769 (GRCm39) D280E probably damaging Het
Krt9 TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC 11: 100,079,903 (GRCm39) probably benign Het
Macf1 T C 4: 123,295,200 (GRCm39) I5344V probably damaging Het
Mcf2l A T 8: 13,053,529 (GRCm39) probably null Het
Mms22l A C 4: 24,505,373 (GRCm39) H211P probably damaging Het
Nipsnap1 A G 11: 4,839,145 (GRCm39) R163G possibly damaging Het
Nusap1 A G 2: 119,477,616 (GRCm39) N375S possibly damaging Het
Or51t4 A G 7: 102,598,278 (GRCm39) Y202C probably damaging Het
Or52e19b A T 7: 103,032,735 (GRCm39) V158D possibly damaging Het
Or8g17 A G 9: 38,930,747 (GRCm39) L30P probably damaging Het
Phf14 G A 6: 11,933,306 (GRCm39) G56D unknown Het
Phykpl G A 11: 51,477,408 (GRCm39) V67M probably damaging Het
Pigk A G 3: 152,453,404 (GRCm39) D374G probably benign Het
Pirb A G 7: 3,722,857 (GRCm39) V45A probably damaging Het
Prm3 CTCTTCTTCTTCTTC CTCTTCTTCTTC 16: 10,608,565 (GRCm39) probably benign Het
Rb1cc1 T C 1: 6,318,786 (GRCm39) V735A probably damaging Het
Rcor2 A G 19: 7,251,225 (GRCm39) T333A possibly damaging Het
Ret A G 6: 118,151,305 (GRCm39) V593A probably benign Het
Scgb1b10 A T 7: 31,800,570 (GRCm39) D53V probably damaging Het
Sec61a2 G A 2: 5,881,794 (GRCm39) A212V probably damaging Het
Sgpl1 A T 10: 60,942,121 (GRCm39) F245I probably damaging Het
Sh2d4b A C 14: 40,542,723 (GRCm39) V351G probably damaging Het
Shld2 C T 14: 33,990,436 (GRCm39) V157I probably benign Het
Slc22a1 T A 17: 12,871,310 (GRCm39) M484L probably benign Het
Slit1 C G 19: 41,599,247 (GRCm39) G1066R probably damaging Het
Slit1 A T 19: 41,599,248 (GRCm39) D1065E probably benign Het
Spg11 G A 2: 121,922,803 (GRCm39) A882V probably damaging Het
Stab2 A C 10: 86,681,897 (GRCm39) F2501L probably benign Het
Stim2 A T 5: 54,275,671 (GRCm39) K607N probably damaging Het
Syde1 C T 10: 78,425,916 (GRCm39) V84M probably damaging Het
Tepsin A T 11: 119,985,061 (GRCm39) N202K probably benign Het
Tmem207 T A 16: 26,335,495 (GRCm39) I81F possibly damaging Het
Tnc C A 4: 63,935,580 (GRCm39) G452V probably damaging Het
Tshz1 G T 18: 84,033,782 (GRCm39) Q209K probably damaging Het
Ttn A T 2: 76,773,696 (GRCm39) N2308K unknown Het
Zfp266 A G 9: 20,410,548 (GRCm39) I543T probably benign Het
Zfp777 G T 6: 48,001,645 (GRCm39) P815Q probably damaging Het
Zhx3 A G 2: 160,623,015 (GRCm39) I384T probably damaging Het
Other mutations in Arhgap25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01454:Arhgap25 APN 6 87,473,058 (GRCm39) missense possibly damaging 0.92
IGL02112:Arhgap25 APN 6 87,444,919 (GRCm39) missense possibly damaging 0.80
IGL03051:Arhgap25 APN 6 87,472,896 (GRCm39) missense probably null 1.00
R0462:Arhgap25 UTSW 6 87,436,942 (GRCm39) missense possibly damaging 0.88
R1636:Arhgap25 UTSW 6 87,472,923 (GRCm39) missense probably damaging 1.00
R1777:Arhgap25 UTSW 6 87,440,289 (GRCm39) missense probably benign 0.41
R2077:Arhgap25 UTSW 6 87,436,990 (GRCm39) missense probably damaging 1.00
R2845:Arhgap25 UTSW 6 87,436,949 (GRCm39) missense possibly damaging 0.86
R4091:Arhgap25 UTSW 6 87,440,017 (GRCm39) missense probably benign
R4435:Arhgap25 UTSW 6 87,439,920 (GRCm39) missense possibly damaging 0.56
R4773:Arhgap25 UTSW 6 87,473,053 (GRCm39) missense probably benign
R5121:Arhgap25 UTSW 6 87,509,846 (GRCm39) missense probably benign 0.01
R5169:Arhgap25 UTSW 6 87,440,252 (GRCm39) missense possibly damaging 0.93
R5334:Arhgap25 UTSW 6 87,440,243 (GRCm39) missense possibly damaging 0.77
R5726:Arhgap25 UTSW 6 87,440,441 (GRCm39) missense probably benign
R6696:Arhgap25 UTSW 6 87,443,545 (GRCm39) missense probably damaging 0.99
R6696:Arhgap25 UTSW 6 87,442,633 (GRCm39) missense probably damaging 1.00
R8113:Arhgap25 UTSW 6 87,465,287 (GRCm39) nonsense probably null
R9110:Arhgap25 UTSW 6 87,453,254 (GRCm39) missense probably benign 0.07
R9500:Arhgap25 UTSW 6 87,469,184 (GRCm39) missense probably damaging 1.00
R9591:Arhgap25 UTSW 6 87,440,102 (GRCm39) missense probably benign
Z1176:Arhgap25 UTSW 6 87,453,168 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ACATTTTGGAAGGTGGGGAC -3'
(R):5'- GTTCTGGTCTCCATCTTCAGAG -3'

Sequencing Primer
(F):5'- GAAGGTGGGGACAGTATCCTTCC -3'
(R):5'- GGTCTCCATCTTCAGAGGCTAAG -3'
Posted On 2020-09-15