Mutagenetix > Incidental Mutation

Incidental Mutation 'R7954:Vmn1r78'

649718

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r78

Ensembl Gene

ENSMUSG00000061602

Gene Name

vomeronasal 1 receptor 78

Synonyms

V1rg7

MMRRC Submission

045998-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R7954 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

11886391-11887332 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 11887227 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 279 (C279*)

Ref Sequence

ENSEMBL: ENSMUSP00000077186 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078039] [ENSMUST00000228244] [ENSMUST00000228664]

AlphaFold

K7N608

Predicted Effect

probably null
Transcript: ENSMUST00000078039
AA Change: C279*

SMART Domains

Protein: ENSMUSP00000077186
Gene: ENSMUSG00000061602
AA Change: C279*

Domain	Start	End	E-Value	Type
Pfam:TAS2R	3	304	5.7e-8	PFAM
Pfam:V1R	12	301	1.5e-29	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000228244
AA Change: C279*

Predicted Effect

probably null
Transcript: ENSMUST00000228664
AA Change: C279*

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	C	T	11: 119,903,169 (GRCm39)	C409Y	possibly damaging	Het
Acsm2	C	T	7: 119,179,952 (GRCm39)	T390I	probably damaging	Het
Adam20	T	C	8: 41,249,581 (GRCm39)	Y564H	probably damaging	Het
Adcy6	A	G	15: 98,494,773 (GRCm39)		probably null	Het
Alms1	T	C	6: 85,598,144 (GRCm39)	V990A	probably damaging	Het
Anapc2	T	A	2: 25,164,712 (GRCm39)	V318E	probably damaging	Het
Ano6	G	T	15: 95,863,702 (GRCm39)	A741S	possibly damaging	Het
Apc	A	G	18: 34,447,321 (GRCm39)	S1406G	probably damaging	Het
Cemip2	T	A	19: 21,770,264 (GRCm39)	I84N	probably damaging	Het
Cfi	G	A	3: 129,662,234 (GRCm39)		probably null	Het
Clcn1	A	T	6: 42,263,625 (GRCm39)		probably benign	Het
Crygs	C	T	16: 22,624,082 (GRCm39)	R175H	probably damaging	Het
Dnmbp	A	T	19: 43,890,742 (GRCm39)	W342R	probably benign	Het
Dok5	T	A	2: 170,674,993 (GRCm39)		probably null	Het
Eno3	T	C	11: 70,552,006 (GRCm39)	I284T	probably benign	Het
Ep400	T	C	5: 110,816,599 (GRCm39)	T2767A	possibly damaging	Het
Epb41l4b	A	G	4: 57,088,034 (GRCm39)	V162A	probably damaging	Het
Fat3	A	G	9: 15,909,708 (GRCm39)	I2098T	probably damaging	Het
Fbxl13	T	C	5: 21,748,767 (GRCm39)	N384S	probably benign	Het
Fbxo9	A	G	9: 78,008,826 (GRCm39)	L78P	probably benign	Het
Glis1	G	A	4: 107,476,854 (GRCm39)	R525H	possibly damaging	Het
Gpr15	T	G	16: 58,539,047 (GRCm39)	D14A	probably benign	Het
Gpr156	T	C	16: 37,807,920 (GRCm39)	I189T	probably damaging	Het
H6pd	T	C	4: 150,067,283 (GRCm39)	I376V	probably benign	Het
Hs6st3	T	C	14: 120,106,522 (GRCm39)	V310A	probably damaging	Het
Htr1b	A	G	9: 81,513,998 (GRCm39)	L203P	probably damaging	Het
Kif26b	A	T	1: 178,696,944 (GRCm39)	I531F	probably damaging	Het
Krtcap3	T	C	5: 31,410,015 (GRCm39)	L166P	probably damaging	Het
Lmod1	A	C	1: 135,252,794 (GRCm39)	D16A	probably damaging	Het
Lrp2	T	G	2: 69,333,867 (GRCm39)	N1458T	possibly damaging	Het
Mocs1	G	A	17: 49,761,799 (GRCm39)	G631E	possibly damaging	Het
Nop14	G	A	5: 34,807,729 (GRCm39)	P411L	probably benign	Het
Nr2f1	A	C	13: 78,338,113 (GRCm39)	D334E	probably damaging	Het
Or5al7	A	G	2: 85,993,212 (GRCm39)	L27P	probably damaging	Het
Or5p51	T	C	7: 107,444,119 (GRCm39)	N274D	probably benign	Het
Paqr9	G	A	9: 95,442,681 (GRCm39)	V224M	probably damaging	Het
Pkd2l1	T	C	19: 44,142,651 (GRCm39)	T464A	probably benign	Het
Prss58	T	C	6: 40,872,543 (GRCm39)	R188G	possibly damaging	Het
Rapgef2	G	A	3: 78,977,454 (GRCm39)	R1150*	probably null	Het
Rgs22	A	C	15: 36,082,148 (GRCm39)	F653V	possibly damaging	Het
Spata2	T	C	2: 167,325,857 (GRCm39)	T321A	probably benign	Het
St3gal1	A	T	15: 66,984,422 (GRCm39)	F118I	probably damaging	Het
Styxl2	C	T	1: 165,926,849 (GRCm39)	S921N	probably benign	Het
Tasor	T	C	14: 27,169,481 (GRCm39)		probably null	Het
Tex35	C	T	1: 156,927,742 (GRCm39)	D143N	probably damaging	Het
Tll1	G	A	8: 64,571,568 (GRCm39)	T171I	probably damaging	Het
Umodl1	A	T	17: 31,205,361 (GRCm39)	Q652L	probably benign	Het
Usp54	A	G	14: 20,611,981 (GRCm39)	L945P	probably benign	Het
Vars1	A	G	17: 35,234,960 (GRCm39)	H1263R	probably benign	Het
Vmn1r202	A	T	13: 22,685,871 (GRCm39)	M182K	probably benign	Het

Other mutations in Vmn1r78

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01129:Vmn1r78	APN	7	11,887,165 (GRCm39)	missense	probably benign	0.10
IGL02019:Vmn1r78	APN	7	11,886,634 (GRCm39)	missense	probably damaging	0.99
IGL02143:Vmn1r78	APN	7	11,886,407 (GRCm39)	missense	probably benign
IGL02154:Vmn1r78	APN	7	11,886,472 (GRCm39)	missense	probably benign	0.22
IGL02290:Vmn1r78	APN	7	11,887,082 (GRCm39)	missense	probably damaging	1.00
IGL03012:Vmn1r78	APN	7	11,887,291 (GRCm39)	missense	probably benign	0.32
IGL03256:Vmn1r78	APN	7	11,886,725 (GRCm39)	missense	probably damaging	1.00
IGL03373:Vmn1r78	APN	7	11,887,270 (GRCm39)	missense	possibly damaging	0.86
IGL03384:Vmn1r78	APN	7	11,887,136 (GRCm39)	missense	possibly damaging	0.94
R0016:Vmn1r78	UTSW	7	11,887,279 (GRCm39)	missense	probably benign	0.02
R1445:Vmn1r78	UTSW	7	11,886,508 (GRCm39)	missense	possibly damaging	0.64
R1748:Vmn1r78	UTSW	7	11,887,250 (GRCm39)	missense	probably damaging	1.00
R2017:Vmn1r78	UTSW	7	11,887,270 (GRCm39)	missense	possibly damaging	0.86
R2032:Vmn1r78	UTSW	7	11,887,210 (GRCm39)	missense	probably benign	0.00
R2198:Vmn1r78	UTSW	7	11,886,487 (GRCm39)	missense	probably benign	0.06
R4330:Vmn1r78	UTSW	7	11,886,386 (GRCm39)	splice site	probably null
R4564:Vmn1r78	UTSW	7	11,886,485 (GRCm39)	missense	probably damaging	1.00
R4769:Vmn1r78	UTSW	7	11,886,725 (GRCm39)	missense	probably damaging	1.00
R4801:Vmn1r78	UTSW	7	11,886,891 (GRCm39)	nonsense	probably null
R4802:Vmn1r78	UTSW	7	11,886,891 (GRCm39)	nonsense	probably null
R4860:Vmn1r78	UTSW	7	11,886,683 (GRCm39)	missense	probably damaging	1.00
R4860:Vmn1r78	UTSW	7	11,886,683 (GRCm39)	missense	probably damaging	1.00
R5648:Vmn1r78	UTSW	7	11,886,693 (GRCm39)	missense	possibly damaging	0.92
R6561:Vmn1r78	UTSW	7	11,886,826 (GRCm39)	missense	probably damaging	1.00
R6869:Vmn1r78	UTSW	7	11,886,676 (GRCm39)	missense	probably benign	0.01
R6945:Vmn1r78	UTSW	7	11,886,832 (GRCm39)	missense	probably benign	0.01
R7793:Vmn1r78	UTSW	7	11,887,241 (GRCm39)	missense	probably benign	0.01
R8698:Vmn1r78	UTSW	7	11,886,539 (GRCm39)	missense	probably benign	0.06
R8830:Vmn1r78	UTSW	7	11,887,118 (GRCm39)	missense	probably damaging	0.98
R9624:Vmn1r78	UTSW	7	11,886,410 (GRCm39)	missense	probably benign
Z1088:Vmn1r78	UTSW	7	11,886,641 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGGTGAGTGTCCTCTACAGACAC -3'
(R):5'- GACTGCAGTCTTAGCATTCTACAATAG -3'

Sequencing Primer
(F):5'- GTCAAGCATATCCATAGTGCTCAG -3'
(R):5'- CTTAGCATTCTACAATAGATACAGGG -3'

Posted On

2020-09-15