Mutagenetix > Incidental Mutation

Incidental Mutation 'R7955:Tex35'

649750

Institutional Source

Beutler Lab

Gene Symbol

Tex35

Ensembl Gene

ENSMUSG00000026592

Gene Name

testis expressed 35

Synonyms

1700057K13Rik

MMRRC Submission

045999-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.051) question?

Stock #

R7955 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

156926709-156936250 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 156927742 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Asparagine at position 143 (D143N)

Ref Sequence

ENSEMBL: ENSMUSP00000027884 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027884] [ENSMUST00000118207] [ENSMUST00000119526] [ENSMUST00000121911] [ENSMUST00000148649] [ENSMUST00000187546]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000027884
AA Change: D143N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000027884
Gene: ENSMUSG00000026592
AA Change: D143N

Domain	Start	End	E-Value	Type
Pfam:DUF4546	1	201	1.7e-116	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000118207
AA Change: D143N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000114092
Gene: ENSMUSG00000026592
AA Change: D143N

Domain	Start	End	E-Value	Type
Pfam:Tsc35	1	201	5.4e-108	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000119526
AA Change: D122N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000113325
Gene: ENSMUSG00000026592
AA Change: D122N

Domain	Start	End	E-Value	Type
Pfam:DUF4546	1	180	6.6e-104	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000121911
AA Change: D122N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000113622
Gene: ENSMUSG00000026592
AA Change: D122N

Domain	Start	End	E-Value	Type
Pfam:DUF4546	1	181	2e-102	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000148649

SMART Domains

Protein: ENSMUSP00000116924
Gene: ENSMUSG00000026592

Domain	Start	End	E-Value	Type
low complexity region	24	34	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000187546
AA Change: D143N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000139460
Gene: ENSMUSG00000026592
AA Change: D143N

Domain	Start	End	E-Value	Type
Pfam:DUF4546	1	183	3e-98	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts10	T	A	17: 33,764,613 (GRCm39)	V711D	probably damaging	Het
Adgrg5	G	A	8: 95,664,325 (GRCm39)	V312M		Het
Ankrd53	A	G	6: 83,744,845 (GRCm39)	T352A	probably benign	Het
Ano4	T	A	10: 88,831,088 (GRCm39)	M512L	probably null	Het
AW551984	A	G	9: 39,507,960 (GRCm39)	F392S	probably damaging	Het
Chrna7	T	G	7: 62,753,541 (GRCm39)	K326T	possibly damaging	Het
Cldnd2	G	T	7: 43,091,120 (GRCm39)	L17F	possibly damaging	Het
Clvs2	T	C	10: 33,471,808 (GRCm39)	N166D	possibly damaging	Het
Cox10	A	T	11: 63,884,750 (GRCm39)	N218K	probably benign	Het
Crygs	C	T	16: 22,624,082 (GRCm39)	R175H	probably damaging	Het
Daxx	T	A	17: 34,131,229 (GRCm39)	Y385*	probably null	Het
Dnmbp	T	C	19: 43,890,762 (GRCm39)	E335G	probably benign	Het
Erich3	C	T	3: 154,444,951 (GRCm39)	Q376*	probably null	Het
Flot2	A	G	11: 77,949,769 (GRCm39)		probably null	Het
Ganc	A	G	2: 120,261,181 (GRCm39)	T289A	probably damaging	Het
Gm19668	G	A	10: 77,634,630 (GRCm39)	T113I	unknown	Het
Gpm6a	A	G	8: 55,511,840 (GRCm39)	N238S	probably damaging	Het
Hps1	T	C	19: 42,759,221 (GRCm39)	T124A	probably damaging	Het
Larp4b	T	C	13: 9,186,816 (GRCm39)	V48A	probably benign	Het
Magi2	A	G	5: 20,594,070 (GRCm39)	H205R	probably damaging	Het
Map2	T	A	1: 66,452,875 (GRCm39)	S588R	probably damaging	Het
Mocos	A	G	18: 24,799,216 (GRCm39)	D150G	probably damaging	Het
Msl2	A	G	9: 100,979,354 (GRCm39)	D576G	possibly damaging	Het
Mybpc3	A	G	2: 90,956,401 (GRCm39)		probably null	Het
Myo3b	A	T	2: 69,925,623 (GRCm39)	Y58F	probably benign	Het
Ndst3	T	G	3: 123,400,586 (GRCm39)	K440T	probably benign	Het
Ndst4	T	A	3: 125,231,831 (GRCm39)	Y133*	probably null	Het
Nrcam	G	A	12: 44,631,737 (GRCm39)	V1097M	probably benign	Het
Or6z1	A	G	7: 6,505,078 (GRCm39)	I49T	possibly damaging	Het
Rabep1	A	G	11: 70,808,267 (GRCm39)	T408A	probably damaging	Het
Rims1	A	T	1: 22,507,322 (GRCm39)	D609E	probably damaging	Het
Sh2d4a	A	G	8: 68,781,907 (GRCm39)	K172E	probably benign	Het
Slc12a9	A	C	5: 137,323,808 (GRCm39)	L369R	probably damaging	Het
Slc30a1	T	C	1: 191,639,395 (GRCm39)	C93R	probably damaging	Het
Tgm3	A	T	2: 129,880,400 (GRCm39)	Y402F	probably benign	Het
Tmem17	T	A	11: 22,468,490 (GRCm39)	I143K	possibly damaging	Het
Tmprss11f	A	C	5: 86,692,682 (GRCm39)	S81A	probably benign	Het
Trp53bp1	G	A	2: 121,066,225 (GRCm39)	P834S	possibly damaging	Het
Trpm4	C	A	7: 44,968,683 (GRCm39)	G417W	probably damaging	Het
Trpm6	A	T	19: 18,831,654 (GRCm39)	R1506S	probably benign	Het
Tspan18	A	G	2: 93,040,305 (GRCm39)	V150A	possibly damaging	Het
Ttc39d	T	C	17: 80,523,352 (GRCm39)	F4L	probably benign	Het
Zdhhc4	A	T	5: 143,307,619 (GRCm39)	L134H	probably damaging	Het
Zfp407	A	T	18: 84,577,416 (GRCm39)	S1232R	probably benign	Het
Zswim2	A	C	2: 83,747,227 (GRCm39)	F353V	probably benign	Het

Other mutations in Tex35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00160:Tex35	APN	1	156,927,326 (GRCm39)	intron	probably benign
IGL01063:Tex35	APN	1	156,932,667 (GRCm39)	splice site	probably benign
R0006:Tex35	UTSW	1	156,927,314 (GRCm39)	missense	possibly damaging	0.95
R0006:Tex35	UTSW	1	156,927,314 (GRCm39)	missense	possibly damaging	0.95
R4595:Tex35	UTSW	1	156,926,909 (GRCm39)	missense	probably benign	0.23
R4855:Tex35	UTSW	1	156,927,295 (GRCm39)	missense	probably damaging	0.99
R5636:Tex35	UTSW	1	156,927,794 (GRCm39)	nonsense	probably null
R5777:Tex35	UTSW	1	156,934,777 (GRCm39)	missense	probably benign	0.32
R7426:Tex35	UTSW	1	156,932,656 (GRCm39)	missense	probably damaging	0.99
R7868:Tex35	UTSW	1	156,926,908 (GRCm39)	nonsense	probably null
R7954:Tex35	UTSW	1	156,927,742 (GRCm39)	missense	probably damaging	0.99
R7956:Tex35	UTSW	1	156,927,742 (GRCm39)	missense	probably damaging	0.99
R9223:Tex35	UTSW	1	156,935,436 (GRCm39)	missense	probably benign	0.00
R9417:Tex35	UTSW	1	156,934,789 (GRCm39)	missense	possibly damaging	0.51

Predicted Primers

PCR Primer
(F):5'- CGGTTTCTAGACTAAGGGCTG -3'
(R):5'- GCTGGAAGCTAAGATCTTCTGTG -3'

Sequencing Primer
(F):5'- TCTAGACTAAGGGCTGCATCC -3'
(R):5'- GAAGCTAAGATCTTCTGTGCCCATG -3'

Posted On

2020-09-15