Mutagenetix > Incidental Mutation

Incidental Mutation 'R7957:Vmn2r88'

649910

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r88

Ensembl Gene

ENSMUSG00000000606

Gene Name

vomeronasal 2, receptor 88

Synonyms

V2r3, V2r13

MMRRC Submission

046001-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.121) question?

Stock #

R7957 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

51410819-51419527 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 51413132 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 101 (M101L)

Ref Sequence

ENSEMBL: ENSMUSP00000125126 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022438] [ENSMUST00000159674] [ENSMUST00000162998] [ENSMUST00000228139]

AlphaFold

L7N1W8

Predicted Effect

probably benign
Transcript: ENSMUST00000022438
AA Change: M101L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000022438
Gene: ENSMUSG00000000606
AA Change: M101L

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	457	8.3e-27	PFAM
Pfam:NCD3G	516	570	1.2e-18	PFAM
Pfam:7tm_3	603	838	1.9e-55	PFAM

Predicted Effect

SMART Domains

Protein: ENSMUSP00000125126
Gene: ENSMUSG00000000606
AA Change: M101L

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	30	408	3.2e-30	PFAM
Pfam:NCD3G	463	516	1.2e-19	PFAM
Pfam:7tm_3	546	785	3.7e-81	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000162998

SMART Domains

Protein: ENSMUSP00000125409
Gene: ENSMUSG00000068399

Domain	Start	End	E-Value	Type
Pfam:Takusan	35	115	2.2e-25	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000163019
AA Change: M76L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000124837
Gene: ENSMUSG00000000606
AA Change: M76L

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	52	399	3.7e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000228139
AA Change: M101L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Meta Mutation Damage Score

0.0852

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (46/47)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	A	G	11: 110,091,613	M1T	probably null	Het
Abhd2	T	A	7: 79,325,446	M128K	probably benign	Het
Adamts12	A	G	15: 11,317,212	T1333A	possibly damaging	Het
Alg8	A	G	7: 97,390,924	T438A	probably benign	Het
Cebpa	A	G	7: 35,120,442	I342V	possibly damaging	Het
Chd9	T	C	8: 91,051,698	M2779T	probably damaging	Het
Cnot3	C	A	7: 3,658,222	P577T	probably benign	Het
Col17a1	T	C	19: 47,661,117	D755G	probably damaging	Het
Col5a3	A	G	9: 20,774,051	V1443A	unknown	Het
Crygs	C	T	16: 22,805,332	R175H	probably damaging	Het
Fam193a	A	G	5: 34,462,086	D1031G	probably damaging	Het
Fam83b	T	A	9: 76,491,985	H612L	probably benign	Het
Gabrr2	A	G	4: 33,081,410	T149A	probably damaging	Het
Gm15922	C	G	7: 3,737,320	A301P	probably damaging	Het
Gm3667	T	C	14: 6,872,332	N156D	probably benign	Het
Hpcal1	T	A	12: 17,791,170	L183Q	probably damaging	Het
Ilf2	G	T	3: 90,487,470	E342*	probably null	Het
Ints13	A	G	6: 146,550,766	S652P	probably damaging	Het
Kansl2	C	A	15: 98,524,618	E356D	probably benign	Het
Klhl6	T	C	16: 19,949,451	E448G	probably null	Het
Mmp14	A	G	14: 54,436,250	I124V	probably benign	Het
Morc2b	A	T	17: 33,135,773	D1008E	probably benign	Het
Muc16	A	C	9: 18,643,471	V3842G	unknown	Het
Myo9b	A	T	8: 71,354,761	I1614F	probably benign	Het
Nipa1	A	T	7: 55,979,799	C189S	probably damaging	Het
Nov	G	T	15: 54,746,338	S78I	possibly damaging	Het
Ntn4	T	C	10: 93,644,473		probably benign	Het
Olfr1274-ps	A	T	2: 90,401,051	Y130F	probably damaging	Het
Olfr146	T	G	9: 39,019,053	I163L	probably benign	Het
Olfr329-ps	A	G	11: 58,542,798	L239P	probably damaging	Het
Olfr358	A	G	2: 37,004,960	I218T	probably benign	Het
Olfr399	A	C	11: 74,054,156	L201R	probably damaging	Het
Pank1	T	A	19: 34,813,696	H528L	probably damaging	Het
Pappa2	A	T	1: 158,761,561	L1698*	probably null	Het
Park7	A	G	4: 150,903,884	S85P	probably damaging	Het
Pik3r4	A	G	9: 105,687,209	D1334G	probably damaging	Het
Rapgef2	T	C	3: 79,214,969	E30G	probably benign	Het
Rhbdf1	A	G	11: 32,210,523	F676L	probably damaging	Het
Rsf1	GGC	GGCCACGGCAGC	7: 97,579,906		probably benign	Het
Scd4	G	T	19: 44,341,248	M219I	probably benign	Het
Slfn5	T	A	11: 82,956,787	I166N	probably benign	Het
Smim22	A	T	16: 5,008,225	D85V	probably damaging	Het
Socs2	T	C	10: 95,414,950	E7G	probably benign	Het
Thumpd2	T	C	17: 81,026,728	E477G	probably benign	Het
Tlr12	C	T	4: 128,616,690	G589D	probably benign	Het
Ttn	A	T	2: 76,764,855	I20317K	probably damaging	Het
Ttyh2	A	G	11: 114,708,864		probably null	Het
Ugt2a3	T	A	5: 87,327,191	D398V	probably damaging	Het
Vmn2r93	A	T	17: 18,325,692	R609*	probably null	Het
Zfp473	C	T	7: 44,732,492	E806K	probably damaging	Het
Zfp93	T	C	7: 24,275,574	L328P	probably damaging	Het

Other mutations in Vmn2r88

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00990:Vmn2r88	APN	14	51413125	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51413060	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51413256	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51416802	missense	possibly damaging	0.59
IGL02308:Vmn2r88	APN	14	51417980	missense	possibly damaging	0.96
IGL02481:Vmn2r88	APN	14	51414154	missense	probably benign
IGL02483:Vmn2r88	APN	14	51414154	missense	probably benign
IGL03241:Vmn2r88	APN	14	51418373	missense	probably benign	0.03
R0052:Vmn2r88	UTSW	14	51418700	missense	possibly damaging	0.88
R0070:Vmn2r88	UTSW	14	51414140	missense	probably benign	0.08
R0799:Vmn2r88	UTSW	14	51414502	missense	possibly damaging	0.61
R0906:Vmn2r88	UTSW	14	51418209	missense	probably damaging	1.00
R1322:Vmn2r88	UTSW	14	51414108	missense	probably damaging	1.00
R1352:Vmn2r88	UTSW	14	51418550	missense	probably damaging	1.00
R1639:Vmn2r88	UTSW	14	51416787	missense	probably damaging	0.98
R1780:Vmn2r88	UTSW	14	51418572	missense	probably damaging	1.00
R1834:Vmn2r88	UTSW	14	51413030	splice site	probably benign
R1911:Vmn2r88	UTSW	14	51418214	missense	probably damaging	1.00
R2113:Vmn2r88	UTSW	14	51418194	missense	probably damaging	1.00
R2120:Vmn2r88	UTSW	14	51413208	missense	probably benign	0.00
R2126:Vmn2r88	UTSW	14	51413807	missense	probably benign	0.01
R2348:Vmn2r88	UTSW	14	51414004	missense	probably benign	0.00
R2881:Vmn2r88	UTSW	14	51418689	missense	probably damaging	0.97
R2884:Vmn2r88	UTSW	14	51413934	missense	probably damaging	1.00
R3081:Vmn2r88	UTSW	14	51418632	missense	probably damaging	0.99
R3933:Vmn2r88	UTSW	14	51413978	missense	probably benign	0.44
R3967:Vmn2r88	UTSW	14	51413190	missense	probably benign	0.06
R4091:Vmn2r88	UTSW	14	51415426	missense	probably damaging	1.00
R4378:Vmn2r88	UTSW	14	51413289	nonsense	probably null
R4397:Vmn2r88	UTSW	14	51417978	missense	probably damaging	1.00
R4418:Vmn2r88	UTSW	14	51418081	missense	probably damaging	1.00
R4609:Vmn2r88	UTSW	14	51418074	missense	probably damaging	0.98
R4647:Vmn2r88	UTSW	14	51418793	missense	probably benign	0.02
R4672:Vmn2r88	UTSW	14	51418155	missense	probably damaging	1.00
R4684:Vmn2r88	UTSW	14	51413334	missense	possibly damaging	0.95
R4686:Vmn2r88	UTSW	14	51413339	missense	probably benign	0.03
R4720:Vmn2r88	UTSW	14	51413245	missense	probably benign	0.01
R5046:Vmn2r88	UTSW	14	51413181	missense	probably benign	0.03
R5063:Vmn2r88	UTSW	14	51411146	missense	probably damaging	0.96
R5619:Vmn2r88	UTSW	14	51413910	missense	probably damaging	0.99
R5652:Vmn2r88	UTSW	14	51418572	missense	probably damaging	0.98
R6020:Vmn2r88	UTSW	14	51418149	nonsense	probably null
R6103:Vmn2r88	UTSW	14	51415369	missense	probably benign	0.17
R6674:Vmn2r88	UTSW	14	51414338	missense	probably benign	0.01
R6799:Vmn2r88	UTSW	14	51413969	missense	probably benign	0.05
R7089:Vmn2r88	UTSW	14	51418643	missense
R7104:Vmn2r88	UTSW	14	51413796	missense
R7265:Vmn2r88	UTSW	14	51418319	missense
R7316:Vmn2r88	UTSW	14	51414255	missense
R7552:Vmn2r88	UTSW	14	51410858	splice site	probably null
R7611:Vmn2r88	UTSW	14	51413997	missense
R7667:Vmn2r88	UTSW	14	51417989	missense
R7682:Vmn2r88	UTSW	14	51418449	missense
R7755:Vmn2r88	UTSW	14	51413046	missense	probably benign	0.00
R7811:Vmn2r88	UTSW	14	51418703	missense
R7882:Vmn2r88	UTSW	14	51413046	missense	probably benign	0.00
R7998:Vmn2r88	UTSW	14	51414108	missense
R8142:Vmn2r88	UTSW	14	51414107	missense
R8186:Vmn2r88	UTSW	14	51418700	missense
R8348:Vmn2r88	UTSW	14	51418796	missense	probably damaging	0.97
R8448:Vmn2r88	UTSW	14	51418796	missense	probably damaging	0.97
R8483:Vmn2r88	UTSW	14	51413073	missense	possibly damaging	0.48
R8783:Vmn2r88	UTSW	14	51414066	missense
R8859:Vmn2r88	UTSW	14	51418806	missense	probably damaging	0.97
R8916:Vmn2r88	UTSW	14	51411136	missense
R8936:Vmn2r88	UTSW	14	51418526	missense	possibly damaging	0.88
R9004:Vmn2r88	UTSW	14	51413167	missense
R9038:Vmn2r88	UTSW	14	51414033	missense
R9063:Vmn2r88	UTSW	14	51410872	start gained	probably benign
R9311:Vmn2r88	UTSW	14	51413046	missense	probably benign	0.00
R9382:Vmn2r88	UTSW	14	51418740	missense
R9483:Vmn2r88	UTSW	14	51411184	missense
R9602:Vmn2r88	UTSW	14	51413732	missense
V5622:Vmn2r88	UTSW	14	51413127	missense	probably benign
X0024:Vmn2r88	UTSW	14	51413832	missense	possibly damaging	0.79
X0025:Vmn2r88	UTSW	14	51416802	missense	possibly damaging	0.59
Z1177:Vmn2r88	UTSW	14	51418046	frame shift	probably null
Z1177:Vmn2r88	UTSW	14	51418187	missense
Z1190:Vmn2r88	UTSW	14	51413201	missense

Predicted Primers

PCR Primer
(F):5'- GTGCTCAGACAACACTTAAAGGAC -3'
(R):5'- TGCATTGCCAGTTTTAAGGATG -3'

Sequencing Primer
(F):5'- AAAATAATTAGTCTGGCTGTGCGG -3'
(R):5'- GTTTTCCATGATGGTCCTGTAAGAC -3'

Posted On

2020-09-15