Mutagenetix > Incidental Mutation

Incidental Mutation 'R7959:Vmn2r27'

650000

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r27

Ensembl Gene

ENSMUSG00000072778

Gene Name

vomeronasal 2, receptor27

Synonyms

EG232367

MMRRC Submission

046003-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R7959 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

124168555-124208743 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 124169040 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glycine at position 697 (R697G)

Ref Sequence

ENSEMBL: ENSMUSP00000098528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100968]

AlphaFold

D3YUK6

Predicted Effect

probably benign
Transcript: ENSMUST00000100968
AA Change: R697G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000098528
Gene: ENSMUSG00000072778
AA Change: R697G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	81	475	1.1e-27	PFAM
Pfam:NCD3G	519	570	1.3e-18	PFAM
Pfam:7tm_3	603	838	2.6e-50	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahnak	T	C	19: 8,988,013 (GRCm39)	V3099A	possibly damaging	Het
Apba2	A	G	7: 64,345,571 (GRCm39)	M254V	probably benign	Het
Bbs7	C	T	3: 36,657,085 (GRCm39)	D248N	probably damaging	Het
Ccdc168	T	C	1: 44,096,728 (GRCm39)	I1457V	probably benign	Het
Cldn8	A	G	16: 88,359,829 (GRCm39)	V32A	probably damaging	Het
Col4a4	G	T	1: 82,484,780 (GRCm39)	P496T	unknown	Het
Cyp27a1	T	A	1: 74,776,236 (GRCm39)	N417K	probably benign	Het
Dnah7a	T	C	1: 53,682,621 (GRCm39)	D283G	probably benign	Het
Efcab3	A	G	11: 104,933,627 (GRCm39)	K4878E	probably damaging	Het
Elp1	A	T	4: 56,774,737 (GRCm39)	M746K	probably damaging	Het
Fmn1	T	A	2: 113,195,967 (GRCm39)	Y556N	unknown	Het
Fsip2	T	C	2: 82,816,120 (GRCm39)	I3951T	possibly damaging	Het
Gigyf1	C	T	5: 137,522,581 (GRCm39)	T773I	probably damaging	Het
Heatr6	A	T	11: 83,672,189 (GRCm39)	K1066*	probably null	Het
Mdfic	T	C	6: 15,741,070 (GRCm39)	S142P	possibly damaging	Het
Mettl16	A	G	11: 74,707,852 (GRCm39)	I389V	probably benign	Het
Mia3	T	C	1: 183,125,760 (GRCm39)	Y57C	probably damaging	Het
Nrp2	C	T	1: 62,784,567 (GRCm39)	R239C	probably damaging	Het
Nup160	T	C	2: 90,544,239 (GRCm39)		probably null	Het
Or1e1	T	C	11: 73,244,744 (GRCm39)	L55P	probably damaging	Het
Or2n1e	A	G	17: 38,586,602 (GRCm39)	*313W	probably null	Het
Or52a24	T	A	7: 103,382,015 (GRCm39)	V294D	probably damaging	Het
Or8b1b	T	A	9: 38,376,211 (GRCm39)	S291R	probably damaging	Het
Or9s13	T	A	1: 92,548,029 (GRCm39)	C134S	probably damaging	Het
Pierce1	A	T	2: 28,352,369 (GRCm39)	N131K	probably damaging	Het
Plcxd1	A	T	5: 110,251,422 (GRCm39)	I333F	probably damaging	Het
Plxna2	C	A	1: 194,493,270 (GRCm39)	S1848R	probably damaging	Het
Plxna2	AT	A	1: 194,476,172 (GRCm39)		probably null	Het
Pnpla2	T	A	7: 141,037,406 (GRCm39)	D136E	probably benign	Het
Polr1b	T	A	2: 128,950,014 (GRCm39)	F245I	probably damaging	Het
Prmt9	T	A	8: 78,287,594 (GRCm39)	I245N	probably damaging	Het
Serhl	A	T	15: 82,986,073 (GRCm39)	D62V	probably damaging	Het
Sh3rf3	A	T	10: 58,842,925 (GRCm39)	D297V	probably damaging	Het
Spata13	C	T	14: 60,993,679 (GRCm39)	R1044*	probably null	Het
Strbp	G	A	2: 37,530,906 (GRCm39)	T116I	probably benign	Het
Supt5	T	C	7: 28,015,224 (GRCm39)	D977G	probably benign	Het
Tmem184c	A	G	8: 78,329,532 (GRCm39)	V176A	possibly damaging	Het
Uhrf2	A	G	19: 30,063,660 (GRCm39)	N541S	probably damaging	Het
Vmn2r24	T	A	6: 123,755,949 (GRCm39)	F7Y	possibly damaging	Het
Zfp994	A	G	17: 22,421,761 (GRCm39)	V18A	probably damaging	Het

Other mutations in Vmn2r27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Vmn2r27	APN	6	124,169,370 (GRCm39)	missense	possibly damaging	0.86
IGL01388:Vmn2r27	APN	6	124,200,791 (GRCm39)	missense	possibly damaging	0.55
IGL01923:Vmn2r27	APN	6	124,177,484 (GRCm39)	missense	probably benign	0.20
IGL01954:Vmn2r27	APN	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
IGL02105:Vmn2r27	APN	6	124,174,308 (GRCm39)	splice site	probably benign
IGL02586:Vmn2r27	APN	6	124,201,434 (GRCm39)	nonsense	probably null
IGL03130:Vmn2r27	APN	6	124,169,276 (GRCm39)	missense	possibly damaging	0.82
IGL03330:Vmn2r27	APN	6	124,207,139 (GRCm39)	nonsense	probably null
R0124:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0384:Vmn2r27	UTSW	6	124,200,871 (GRCm39)	missense	probably benign	0.01
R0582:Vmn2r27	UTSW	6	124,201,249 (GRCm39)	missense	probably benign	0.02
R0733:Vmn2r27	UTSW	6	124,169,147 (GRCm39)	missense	probably benign	0.18
R0738:Vmn2r27	UTSW	6	124,200,661 (GRCm39)	missense	possibly damaging	0.48
R0835:Vmn2r27	UTSW	6	124,177,583 (GRCm39)	missense	probably damaging	0.99
R1183:Vmn2r27	UTSW	6	124,177,491 (GRCm39)	missense	probably benign
R1401:Vmn2r27	UTSW	6	124,168,591 (GRCm39)	nonsense	probably null
R1484:Vmn2r27	UTSW	6	124,177,474 (GRCm39)	missense	probably damaging	0.96
R1536:Vmn2r27	UTSW	6	124,177,649 (GRCm39)	missense	probably damaging	1.00
R1539:Vmn2r27	UTSW	6	124,168,730 (GRCm39)	missense	probably damaging	1.00
R1565:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1595:Vmn2r27	UTSW	6	124,208,574 (GRCm39)	missense	probably benign	0.00
R1614:Vmn2r27	UTSW	6	124,200,893 (GRCm39)	missense	probably benign	0.01
R1742:Vmn2r27	UTSW	6	124,177,636 (GRCm39)	missense	possibly damaging	0.48
R1816:Vmn2r27	UTSW	6	124,207,330 (GRCm39)	nonsense	probably null
R1822:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1824:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1870:Vmn2r27	UTSW	6	124,201,170 (GRCm39)	missense	probably benign	0.11
R1942:Vmn2r27	UTSW	6	124,200,722 (GRCm39)	missense	probably damaging	1.00
R1962:Vmn2r27	UTSW	6	124,200,793 (GRCm39)	missense	possibly damaging	0.70
R2069:Vmn2r27	UTSW	6	124,201,442 (GRCm39)	missense	probably damaging	1.00
R2075:Vmn2r27	UTSW	6	124,177,510 (GRCm39)	missense	possibly damaging	0.85
R2379:Vmn2r27	UTSW	6	124,201,342 (GRCm39)	missense	possibly damaging	0.89
R3748:Vmn2r27	UTSW	6	124,207,351 (GRCm39)	missense	probably benign	0.35
R4384:Vmn2r27	UTSW	6	124,201,115 (GRCm39)	missense	probably benign	0.05
R4392:Vmn2r27	UTSW	6	124,207,135 (GRCm39)	missense	probably benign	0.01
R4758:Vmn2r27	UTSW	6	124,208,596 (GRCm39)	missense	possibly damaging	0.87
R5018:Vmn2r27	UTSW	6	124,201,141 (GRCm39)	missense	probably benign	0.02
R5235:Vmn2r27	UTSW	6	124,169,013 (GRCm39)	missense	probably damaging	0.99
R5718:Vmn2r27	UTSW	6	124,169,103 (GRCm39)	missense	possibly damaging	0.66
R5859:Vmn2r27	UTSW	6	124,177,647 (GRCm39)	missense	probably damaging	1.00
R5958:Vmn2r27	UTSW	6	124,208,686 (GRCm39)	missense	probably benign	0.00
R6044:Vmn2r27	UTSW	6	124,208,731 (GRCm39)	missense	probably benign
R6086:Vmn2r27	UTSW	6	124,168,958 (GRCm39)	missense	probably damaging	1.00
R6396:Vmn2r27	UTSW	6	124,201,125 (GRCm39)	nonsense	probably null
R6546:Vmn2r27	UTSW	6	124,169,369 (GRCm39)	missense	possibly damaging	0.49
R6746:Vmn2r27	UTSW	6	124,177,552 (GRCm39)	missense	possibly damaging	0.47
R6976:Vmn2r27	UTSW	6	124,201,312 (GRCm39)	nonsense	probably null
R7091:Vmn2r27	UTSW	6	124,200,904 (GRCm39)	missense	possibly damaging	0.85
R7145:Vmn2r27	UTSW	6	124,168,711 (GRCm39)	missense	probably benign
R7176:Vmn2r27	UTSW	6	124,168,995 (GRCm39)	missense	probably benign	0.01
R7382:Vmn2r27	UTSW	6	124,174,276 (GRCm39)	missense	probably damaging	1.00
R7482:Vmn2r27	UTSW	6	124,201,220 (GRCm39)	missense	probably damaging	1.00
R7853:Vmn2r27	UTSW	6	124,168,980 (GRCm39)	missense	probably damaging	1.00
R7859:Vmn2r27	UTSW	6	124,201,201 (GRCm39)	missense	probably benign	0.00
R8266:Vmn2r27	UTSW	6	124,168,937 (GRCm39)	missense	probably benign	0.00
R8353:Vmn2r27	UTSW	6	124,169,404 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r27	UTSW	6	124,168,776 (GRCm39)	missense	possibly damaging	0.71
R8463:Vmn2r27	UTSW	6	124,169,168 (GRCm39)	missense	probably damaging	1.00
R8477:Vmn2r27	UTSW	6	124,201,200 (GRCm39)	missense	probably benign	0.11
R8705:Vmn2r27	UTSW	6	124,207,188 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r27	UTSW	6	124,201,018 (GRCm39)	missense	probably benign	0.00
R9109:Vmn2r27	UTSW	6	124,174,224 (GRCm39)	missense	possibly damaging	0.95
R9140:Vmn2r27	UTSW	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
R9157:Vmn2r27	UTSW	6	124,201,244 (GRCm39)	missense	probably benign	0.09
R9431:Vmn2r27	UTSW	6	124,168,856 (GRCm39)	missense	probably damaging	1.00
R9477:Vmn2r27	UTSW	6	124,168,910 (GRCm39)	missense	probably damaging	0.99
R9758:Vmn2r27	UTSW	6	124,168,637 (GRCm39)	missense	possibly damaging	0.89
Z1177:Vmn2r27	UTSW	6	124,168,860 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAATAAACAAGCTGAGACTGGC -3'
(R):5'- GACACACCCATTGTCAGAGC -3'

Sequencing Primer
(F):5'- AGTACCCTAGAACACAATAGAAGG -3'
(R):5'- CAGAAATCTCAGTTATCTCCTCCTAG -3'

Posted On

2020-09-15