Incidental Mutation 'R7968:Letm2'
ID 650465
Institutional Source Beutler Lab
Gene Symbol Letm2
Ensembl Gene ENSMUSG00000037363
Gene Name leucine zipper-EF-hand containing transmembrane protein 2
Synonyms D030041N04Rik
MMRRC Submission 046011-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.103) question?
Stock # R7968 (G1)
Quality Score 225.009
Status Validated
Chromosome 8
Chromosomal Location 26068506-26087598 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to T at 26083766 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glycine to Aspartic acid at position 155 (G155D)
Ref Sequence ENSEMBL: ENSMUSP00000078160 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000079160] [ENSMUST00000210234] [ENSMUST00000210616] [ENSMUST00000210810] [ENSMUST00000211422]
AlphaFold Q7TNU7
Predicted Effect probably damaging
Transcript: ENSMUST00000079160
AA Change: G155D

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000078160
Gene: ENSMUSG00000037363
AA Change: G155D

DomainStartEndE-ValueType
low complexity region 106 117 N/A INTRINSIC
Pfam:LETM1 120 384 1.2e-102 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000210234
AA Change: G155D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
Predicted Effect probably damaging
Transcript: ENSMUST00000210616
AA Change: G155D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
Predicted Effect probably damaging
Transcript: ENSMUST00000210810
AA Change: G155D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
Predicted Effect probably null
Transcript: ENSMUST00000211422
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.9%
Validation Efficiency 100% (47/47)
Allele List at MGI
Other mutations in this stock
Total: 48 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930590J08Rik A G 6: 91,922,441 (GRCm39) T35A Het
Adamts16 A C 13: 70,886,701 (GRCm39) S1030A probably benign Het
Adgrv1 A T 13: 81,588,344 (GRCm39) V4414E possibly damaging Het
Aff4 C T 11: 53,300,175 (GRCm39) T1026I probably damaging Het
Arfgef1 T C 1: 10,243,145 (GRCm39) Y1065C probably damaging Het
Arhgef3 G T 14: 27,108,070 (GRCm39) D192Y probably damaging Het
Arhgef3 G A 14: 27,116,062 (GRCm39) R268Q probably damaging Het
Atp6v0e T A 17: 26,913,885 (GRCm39) probably null Het
B3galt9 A G 2: 34,728,257 (GRCm39) N19D probably damaging Het
Cenpc1 A G 5: 86,181,551 (GRCm39) Y605H probably benign Het
Cftr T C 6: 18,226,048 (GRCm39) V332A probably benign Het
Csmd2 A G 4: 128,091,118 (GRCm39) H219R Het
Csnk1g3 C T 18: 54,028,726 (GRCm39) probably benign Het
Cyp4f17 T C 17: 32,743,116 (GRCm39) V263A possibly damaging Het
Dao AGG AG 5: 114,153,270 (GRCm39) probably benign Het
Entrep1 G T 19: 23,962,091 (GRCm39) T304K probably damaging Het
Evl C T 12: 108,647,783 (GRCm39) R295* probably null Het
Gstm4 A T 3: 107,951,677 (GRCm39) M35K probably damaging Het
Kcna2 A G 3: 107,012,460 (GRCm39) Y347C possibly damaging Het
Kcnh7 G A 2: 62,566,444 (GRCm39) T829M probably damaging Het
Kcnu1 T C 8: 26,400,898 (GRCm39) V682A probably benign Het
Kctd17 CAGCTGGAGGAGC CAGC 15: 78,321,113 (GRCm39) probably benign Het
Kera A G 10: 97,444,821 (GRCm39) E60G possibly damaging Het
Lrfn4 G A 19: 4,663,343 (GRCm39) A397V probably benign Het
Lrp4 A G 2: 91,324,424 (GRCm39) Q1253R possibly damaging Het
Mob1a T A 6: 83,315,287 (GRCm39) M145K probably benign Het
Nab1 A G 1: 52,529,295 (GRCm39) C201R probably damaging Het
Nlrp9b A G 7: 19,762,493 (GRCm39) E710G probably benign Het
Oas2 A G 5: 120,876,437 (GRCm39) V502A probably benign Het
Or1e26 T C 11: 73,480,154 (GRCm39) S137G probably benign Het
Or52d3 T A 7: 104,229,595 (GRCm39) C247* probably null Het
Or52h7 A T 7: 104,213,857 (GRCm39) H143L probably benign Het
Parg A T 14: 31,936,327 (GRCm39) H494L possibly damaging Het
Phf20 T A 2: 156,135,464 (GRCm39) D600E probably benign Het
Ppef2 G A 5: 92,397,022 (GRCm39) R118C probably damaging Het
Ptprz1 T A 6: 22,959,675 (GRCm39) N57K probably damaging Het
Rrbp1 A T 2: 143,832,081 (GRCm39) S29T probably damaging Het
Sema3g C A 14: 30,942,605 (GRCm39) R69S probably damaging Het
Slc12a4 G A 8: 106,678,237 (GRCm39) R319W possibly damaging Het
Slc4a3 A G 1: 75,528,007 (GRCm39) T419A probably benign Het
Smc3 T C 19: 53,611,649 (GRCm39) probably null Het
Sntg1 C T 1: 8,535,760 (GRCm39) W288* probably null Het
Synpo2l G T 14: 20,716,870 (GRCm39) probably null Het
Tap1 A G 17: 34,413,886 (GRCm39) I634V probably damaging Het
Tmem25 A G 9: 44,706,983 (GRCm39) S278P probably benign Het
Togaram2 T C 17: 72,024,428 (GRCm39) S871P probably benign Het
Trim37 T C 11: 87,040,179 (GRCm39) V225A possibly damaging Het
Tubg1 G T 11: 101,014,854 (GRCm39) A199S probably benign Het
Other mutations in Letm2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02738:Letm2 APN 8 26,076,789 (GRCm39) missense probably damaging 1.00
selbstlob UTSW 8 26,083,977 (GRCm39) missense probably benign 0.03
IGL03098:Letm2 UTSW 8 26,071,745 (GRCm39) missense possibly damaging 0.73
R0062:Letm2 UTSW 8 26,077,464 (GRCm39) splice site probably benign
R0062:Letm2 UTSW 8 26,077,464 (GRCm39) splice site probably benign
R0207:Letm2 UTSW 8 26,068,786 (GRCm39) missense probably damaging 0.96
R0485:Letm2 UTSW 8 26,082,574 (GRCm39) missense probably damaging 1.00
R1869:Letm2 UTSW 8 26,071,729 (GRCm39) missense probably damaging 0.98
R1870:Letm2 UTSW 8 26,086,460 (GRCm39) splice site probably benign
R1871:Letm2 UTSW 8 26,086,460 (GRCm39) splice site probably benign
R3881:Letm2 UTSW 8 26,083,884 (GRCm39) nonsense probably null
R4115:Letm2 UTSW 8 26,070,343 (GRCm39) nonsense probably null
R4459:Letm2 UTSW 8 26,076,715 (GRCm39) missense probably damaging 1.00
R4461:Letm2 UTSW 8 26,076,715 (GRCm39) missense probably damaging 1.00
R4961:Letm2 UTSW 8 26,084,108 (GRCm39) missense possibly damaging 0.86
R5063:Letm2 UTSW 8 26,071,795 (GRCm39) missense probably benign 0.26
R5069:Letm2 UTSW 8 26,083,980 (GRCm39) nonsense probably null
R5732:Letm2 UTSW 8 26,077,341 (GRCm39) missense possibly damaging 0.51
R6527:Letm2 UTSW 8 26,082,522 (GRCm39) utr 3 prime probably benign
R6706:Letm2 UTSW 8 26,083,977 (GRCm39) missense probably benign 0.03
R7624:Letm2 UTSW 8 26,082,553 (GRCm39) nonsense probably null
R8272:Letm2 UTSW 8 26,076,672 (GRCm39) missense probably damaging 1.00
R8356:Letm2 UTSW 8 26,071,729 (GRCm39) missense probably damaging 0.98
R8456:Letm2 UTSW 8 26,071,729 (GRCm39) missense probably damaging 0.98
R8481:Letm2 UTSW 8 26,070,375 (GRCm39) missense possibly damaging 0.86
R9023:Letm2 UTSW 8 26,077,236 (GRCm39) missense
R9234:Letm2 UTSW 8 26,084,102 (GRCm39) missense probably benign 0.03
R9366:Letm2 UTSW 8 26,084,165 (GRCm39) missense probably damaging 1.00
R9636:Letm2 UTSW 8 26,083,719 (GRCm39) missense probably benign 0.33
R9690:Letm2 UTSW 8 26,077,435 (GRCm39) missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- ACACATCCTGCTAGATCCCG -3'
(R):5'- ACATGTCCCTGGCAAACCTC -3'

Sequencing Primer
(F):5'- TGCTTAGATGCTCAAGGCC -3'
(R):5'- TGGCAAACCTCAGCTGGAG -3'
Posted On 2020-09-15