Incidental Mutation 'R7973:Clec4b2'
ID 650707
Institutional Source Beutler Lab
Gene Symbol Clec4b2
Ensembl Gene ENSMUSG00000067767
Gene Name C-type lectin domain family 4, member b2
Synonyms mDCAR1, F830043G12Rik
MMRRC Submission 046016-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.050) question?
Stock # R7973 (G1)
Quality Score 225.009
Status Validated
Chromosome 6
Chromosomal Location 123149852-123181630 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 123181148 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Leucine at position 178 (I178L)
Ref Sequence ENSEMBL: ENSMUSP00000085802 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000088455]
AlphaFold Q67DU8
Predicted Effect probably benign
Transcript: ENSMUST00000088455
AA Change: I178L

PolyPhen 2 Score 0.048 (Sensitivity: 0.94; Specificity: 0.83)
SMART Domains Protein: ENSMUSP00000085802
Gene: ENSMUSG00000067767
AA Change: I178L

DomainStartEndE-ValueType
transmembrane domain 21 43 N/A INTRINSIC
CLECT 79 202 1.87e-33 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 98% (53/54)
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2210016L21Rik A G 5: 115,084,112 (GRCm39) D76G probably benign Het
Abcg1 T C 17: 31,323,132 (GRCm39) V237A probably damaging Het
Adgrv1 A T 13: 81,588,344 (GRCm39) V4414E possibly damaging Het
Akap11 A T 14: 78,752,506 (GRCm39) H122Q Het
Amotl2 C T 9: 102,600,968 (GRCm39) T345I probably benign Het
Arhgef4 C T 1: 34,763,518 (GRCm39) R925W possibly damaging Het
Arid1a C T 4: 133,480,381 (GRCm39) G181D probably damaging Het
Ash1l T A 3: 88,960,164 (GRCm39) S2299T probably benign Het
Atf7ip C T 6: 136,538,062 (GRCm39) Q432* probably null Het
B430306N03Rik T A 17: 48,623,483 (GRCm39) S10T probably benign Het
Bckdk A G 7: 127,505,539 (GRCm39) I221V probably benign Het
Brinp2 T C 1: 158,074,057 (GRCm39) Y688C probably damaging Het
Caml C T 13: 55,779,784 (GRCm39) L286F probably benign Het
Ccdc125 C T 13: 100,806,331 (GRCm39) probably benign Het
Cdh24 A T 14: 54,876,477 (GRCm39) V116E possibly damaging Het
Cenpe T A 3: 134,929,011 (GRCm39) I305N probably damaging Het
Cep41 C T 6: 30,680,130 (GRCm39) G33S probably damaging Het
Dao AGG AG 5: 114,153,270 (GRCm39) probably benign Het
Dapk1 T A 13: 60,909,377 (GRCm39) L1330Q probably damaging Het
Evl C T 12: 108,647,783 (GRCm39) R295* probably null Het
Fmnl1 G T 11: 103,061,984 (GRCm39) probably benign Het
Galk1 A G 11: 115,903,435 (GRCm39) F24L possibly damaging Het
Hyal4 T A 6: 24,755,785 (GRCm39) M1K probably null Het
Ighe T C 12: 113,236,677 (GRCm39) T64A Het
Iqch A G 9: 63,432,228 (GRCm39) I389T possibly damaging Het
Ism2 G T 12: 87,333,769 (GRCm39) T92K possibly damaging Het
Kctd17 CAGCTGGAGGAGC CAGC 15: 78,321,113 (GRCm39) probably benign Het
Kdm1b T C 13: 47,230,922 (GRCm39) S650P probably benign Het
Mapkapk5 T G 5: 121,663,776 (GRCm39) N426T possibly damaging Het
Mroh5 G T 15: 73,664,614 (GRCm39) Y90* probably null Het
Ncan T G 8: 70,550,225 (GRCm39) I1184L probably benign Het
Nectin3 T C 16: 46,216,484 (GRCm39) T439A probably benign Het
Neo1 G T 9: 58,897,476 (GRCm39) A160E probably damaging Het
Nosip T C 7: 44,726,784 (GRCm39) S295P possibly damaging Het
Nup205 T A 6: 35,222,274 (GRCm39) F1979L probably benign Het
Nxpe2 A T 9: 48,231,168 (GRCm39) D400E probably damaging Het
Or4a73 T A 2: 89,421,331 (GRCm39) I43F probably damaging Het
Pfkfb4 G A 9: 108,854,179 (GRCm39) R351Q probably damaging Het
Psg29 T C 7: 16,944,462 (GRCm39) I324T probably benign Het
Ptprh G T 7: 4,583,887 (GRCm39) T235N possibly damaging Het
Ralgapa2 T A 2: 146,230,481 (GRCm39) N981I possibly damaging Het
Ralgps1 T C 2: 33,036,651 (GRCm39) Y421C probably damaging Het
Sf3b3 T C 8: 111,542,922 (GRCm39) E845G possibly damaging Het
Sgk1 G T 10: 21,870,054 (GRCm39) S31I probably benign Het
Sim1 T G 10: 50,857,419 (GRCm39) Y390D probably damaging Het
Slain2 T A 5: 73,112,779 (GRCm39) Y271* probably null Het
Slc12a4 G A 8: 106,678,237 (GRCm39) R319W possibly damaging Het
St6gal1 G A 16: 23,176,585 (GRCm39) A393T probably benign Het
Stab1 A T 14: 30,881,590 (GRCm39) probably null Het
Supv3l1 A T 10: 62,285,202 (GRCm39) L88Q probably damaging Het
Syt2 C A 1: 134,668,570 (GRCm39) probably null Het
Tpr C T 1: 150,279,638 (GRCm39) R256C probably damaging Het
Tubg1 G T 11: 101,014,854 (GRCm39) A199S probably benign Het
Usp32 A G 11: 84,913,634 (GRCm39) I899T probably damaging Het
Usp6nl G T 2: 6,413,762 (GRCm39) R96M probably damaging Het
Vmn1r75 A G 7: 11,614,961 (GRCm39) D231G probably benign Het
Vmn2r42 T C 7: 8,197,872 (GRCm39) E249G probably benign Het
Zbtb34 C T 2: 33,301,531 (GRCm39) G337R probably benign Het
Zfp457 T A 13: 67,441,882 (GRCm39) H231L probably benign Het
Zfp715 C T 7: 42,949,321 (GRCm39) G213D possibly damaging Het
Zfp760 T G 17: 21,941,084 (GRCm39) H86Q probably benign Het
Other mutations in Clec4b2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00956:Clec4b2 APN 6 123,179,110 (GRCm39) nonsense probably null
IGL01753:Clec4b2 APN 6 123,179,169 (GRCm39) missense possibly damaging 0.90
IGL02168:Clec4b2 APN 6 123,181,156 (GRCm39) missense probably damaging 0.98
IGL02388:Clec4b2 APN 6 123,179,187 (GRCm39) splice site probably null
IGL03194:Clec4b2 APN 6 123,177,946 (GRCm39) missense probably benign 0.07
P0041:Clec4b2 UTSW 6 123,158,253 (GRCm39) missense possibly damaging 0.72
R0013:Clec4b2 UTSW 6 123,179,108 (GRCm39) missense probably damaging 1.00
R0121:Clec4b2 UTSW 6 123,181,131 (GRCm39) missense probably benign 0.02
R0401:Clec4b2 UTSW 6 123,158,259 (GRCm39) nonsense probably null
R1072:Clec4b2 UTSW 6 123,181,233 (GRCm39) missense probably damaging 0.99
R2520:Clec4b2 UTSW 6 123,177,942 (GRCm39) missense probably damaging 1.00
R4575:Clec4b2 UTSW 6 123,150,639 (GRCm39) missense probably damaging 0.99
R4897:Clec4b2 UTSW 6 123,177,999 (GRCm39) nonsense probably null
R4898:Clec4b2 UTSW 6 123,181,163 (GRCm39) missense probably benign 0.36
R5022:Clec4b2 UTSW 6 123,177,915 (GRCm39) missense probably null 1.00
R5023:Clec4b2 UTSW 6 123,177,915 (GRCm39) missense probably null 1.00
R5057:Clec4b2 UTSW 6 123,177,915 (GRCm39) missense probably null 1.00
R5404:Clec4b2 UTSW 6 123,158,308 (GRCm39) missense probably benign 0.06
R5508:Clec4b2 UTSW 6 123,150,001 (GRCm39) start gained probably benign
R6082:Clec4b2 UTSW 6 123,181,100 (GRCm39) critical splice acceptor site probably null
R6333:Clec4b2 UTSW 6 123,177,637 (GRCm39) splice site probably null
R6902:Clec4b2 UTSW 6 123,177,987 (GRCm39) nonsense probably null
R6946:Clec4b2 UTSW 6 123,177,987 (GRCm39) nonsense probably null
R7144:Clec4b2 UTSW 6 123,158,343 (GRCm39) missense probably benign 0.02
R7709:Clec4b2 UTSW 6 123,149,974 (GRCm39) start gained probably benign
R8810:Clec4b2 UTSW 6 123,158,269 (GRCm39) missense probably benign 0.23
R9278:Clec4b2 UTSW 6 123,181,224 (GRCm39) missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- AGCTATGAATGATGGAGAACACTTG -3'
(R):5'- GTATAACCATTTGCTGCCTACTTAC -3'

Sequencing Primer
(F):5'- TGATGGAGAACACTTGATTTGTAC -3'
(R):5'- TCTCTGGACATATCTTCCACATATAG -3'
Posted On 2020-09-15