|Institutional Source||Beutler Lab|
|Gene Name||signal transducer and activator of transcription 4|
|Is this an essential gene?||Possibly non essential (E-score: 0.445)|
|Stock #||R0329 (G1)|
|Chromosomal Location||51987148-52107189 bp(+) (GRCm38)|
|Type of Mutation||intron|
|DNA Base Change (assembly)||A to G at 52090870 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000130713 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000027277] [ENSMUST00000168302]|
|AlphaFold||no structure available at present|
|Coding Region Coverage||
|Validation Efficiency||99% (107/108)|
FUNCTION: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Homozygous knockout mice for this gene exhibit reduced inflammation and cytokine production in response to immune challenge. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
PHENOTYPE: Homozygous inactivation of this gene leads to altered cytokine production of T-cells, impaired IL-12 responses, enhanced Th2 cell development, decreased susceptibility to autoimmune diabetes, altered NK cell responses during viral infection, and increased susceptibility to Salmonella infection. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Stat4||
(F):5'- TTGGCAACATCTAGGGCATCATAATCAG -3'
(R):5'- CGAAGAAGGAAGCTCCTGTTCCTC -3'
(F):5'- TCATAATCAGGAGCCAAGCG -3'
(R):5'- TGTTCCTCCCAAAGCTGAAG -3'