Mutagenetix > Incidental Mutation

Incidental Mutation 'R7975:Vmn2r59'

650844

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r59

Ensembl Gene

ENSMUSG00000092032

Gene Name

vomeronasal 2, receptor 59

Synonyms

EG628444

MMRRC Submission

046018-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R7975 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

41661216-41708405 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 41693199 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 467 (D467G)

Ref Sequence

ENSEMBL: ENSMUSP00000131856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168489]

AlphaFold

E9PUT5

Predicted Effect

probably damaging
Transcript: ENSMUST00000168489
AA Change: D467G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000131856
Gene: ENSMUSG00000092032
AA Change: D467G

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.8e-44	PFAM
Pfam:NCD3G	514	567	4.3e-23	PFAM
Pfam:7tm_3	600	835	5.4e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.3%

Validation Efficiency

100% (53/53)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano4	T	C	10: 88,952,847 (GRCm39)	I60V	possibly damaging	Het
Ano5	T	A	7: 51,216,286 (GRCm39)	M381K	probably null	Het
Aox1	T	A	1: 58,348,187 (GRCm39)	I635K	probably benign	Het
Bdp1	C	T	13: 100,156,884 (GRCm39)	C2436Y	probably benign	Het
Bod1l	T	C	5: 41,973,620 (GRCm39)	R2565G	possibly damaging	Het
Brd2	A	T	17: 34,334,424 (GRCm39)	I245N	probably damaging	Het
Capn9	G	A	8: 125,325,515 (GRCm39)	V258M	probably damaging	Het
Ccar2	C	G	14: 70,380,918 (GRCm39)	C324S	possibly damaging	Het
Cd209b	A	T	8: 3,975,948 (GRCm39)	I71N	probably benign	Het
Cdk12	A	T	11: 98,111,928 (GRCm39)	I729F	unknown	Het
Celf1	T	G	2: 90,831,423 (GRCm39)	V84G	probably damaging	Het
Cgn	G	T	3: 94,671,836 (GRCm39)	A965E	probably benign	Het
Cgnl1	T	C	9: 71,632,604 (GRCm39)	E249G	probably benign	Het
Cspg4b	A	G	13: 113,455,841 (GRCm39)	H629R		Het
Cyp2j8	T	C	4: 96,358,776 (GRCm39)	N381S	possibly damaging	Het
Dmtf1	T	C	5: 9,179,169 (GRCm39)	D343G	probably damaging	Het
Fhl5	T	A	4: 25,214,730 (GRCm39)	I16F	probably benign	Het
Gatd1	A	G	7: 140,989,781 (GRCm39)	F143S	probably damaging	Het
Gbp10	C	T	5: 105,368,967 (GRCm39)	G291R	probably benign	Het
Gm28168	T	C	1: 117,875,820 (GRCm39)	Y150H	probably benign	Het
Gm3543	T	C	14: 41,802,122 (GRCm39)	M121V	probably benign	Het
Gramd2b	A	G	18: 56,618,451 (GRCm39)	T220A	probably benign	Het
Gstcd	A	G	3: 132,777,863 (GRCm39)	L316P	probably damaging	Het
Hspg2	G	A	4: 137,282,532 (GRCm39)	G3424D	probably benign	Het
Ifi209	C	T	1: 173,468,722 (GRCm39)	S184F	probably benign	Het
Ino80	A	T	2: 119,286,948 (GRCm39)		probably null	Het
Kcnd3	G	A	3: 105,366,310 (GRCm39)	R60H	probably damaging	Het
Kif21b	T	C	1: 136,098,911 (GRCm39)	C1400R	probably damaging	Het
Lama3	C	T	18: 12,670,796 (GRCm39)	P794L	probably damaging	Het
Lgals4	A	G	7: 28,540,346 (GRCm39)	I214V	probably benign	Het
Lipo4	A	T	19: 33,490,028 (GRCm39)	L158Q	probably damaging	Het
Ltv1	A	G	10: 13,066,453 (GRCm39)	Y58H	probably damaging	Het
Mitf	T	C	6: 97,994,990 (GRCm39)	S479P	probably benign	Het
Mthfr	A	T	4: 148,127,920 (GRCm39)	D132V	probably damaging	Het
Mtrr	T	C	13: 68,727,666 (GRCm39)		probably null	Het
Neb	T	A	2: 52,050,678 (GRCm39)	H6660L	probably benign	Het
Nhlrc3	A	T	3: 53,360,966 (GRCm39)	V263E	probably damaging	Het
Oas1h	G	A	5: 121,009,890 (GRCm39)	V322M	probably damaging	Het
Or10a3n	A	T	7: 108,493,019 (GRCm39)	Y203*	probably null	Het
Or2m13	T	A	16: 19,226,301 (GRCm39)	D155V	probably damaging	Het
Or4a15	C	T	2: 89,193,413 (GRCm39)	R120H	probably benign	Het
Pappa	A	T	4: 65,212,705 (GRCm39)	D1121V	probably damaging	Het
Pax5	A	G	4: 44,537,465 (GRCm39)	S352P	probably damaging	Het
Piezo1	C	A	8: 123,222,504 (GRCm39)	R915L		Het
R3hcc1	T	C	14: 69,944,593 (GRCm39)	D7G	probably damaging	Het
Rasgrp2	A	G	19: 6,458,589 (GRCm39)	K429E	probably damaging	Het
Scn10a	A	G	9: 119,501,286 (GRCm39)	F166S	probably benign	Het
Scn9a	T	G	2: 66,314,597 (GRCm39)	N1707T	probably damaging	Het
Sec23ip	T	C	7: 128,364,201 (GRCm39)	F493S	probably damaging	Het
Slc1a7	G	A	4: 107,869,473 (GRCm39)	V513M	probably benign	Het
Slfn4	A	T	11: 83,077,982 (GRCm39)	I257F	possibly damaging	Het
Smarcal1	T	A	1: 72,652,150 (GRCm39)	D637E	probably benign	Het
Smpd3	A	C	8: 106,982,254 (GRCm39)	C617G	probably benign	Het
Sos2	A	T	12: 69,639,814 (GRCm39)	F990L	probably benign	Het
Spata31h1	C	T	10: 82,119,823 (GRCm39)	A4396T	possibly damaging	Het
Syne1	T	C	10: 4,981,786 (GRCm39)	T844A	probably benign	Het
Tph1	G	A	7: 46,306,678 (GRCm39)	R145C	probably damaging	Het
Ubap2l	G	A	3: 89,946,076 (GRCm39)		probably null	Het
Vmn2r50	T	C	7: 9,771,272 (GRCm39)	I810V	probably benign	Het
Zfp618	T	A	4: 63,049,352 (GRCm39)	N417K	possibly damaging	Het

Other mutations in Vmn2r59

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Vmn2r59	APN	7	41,661,488 (GRCm39)	missense	possibly damaging	0.91
IGL01432:Vmn2r59	APN	7	41,661,983 (GRCm39)	missense	possibly damaging	0.82
IGL02119:Vmn2r59	APN	7	41,695,593 (GRCm39)	missense	probably benign	0.36
IGL02216:Vmn2r59	APN	7	41,661,817 (GRCm39)	missense	probably damaging	1.00
IGL02327:Vmn2r59	APN	7	41,661,655 (GRCm39)	missense	probably benign
IGL03346:Vmn2r59	APN	7	41,693,253 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r59	APN	7	41,708,340 (GRCm39)	missense	probably benign	0.43
IGL03412:Vmn2r59	APN	7	41,661,862 (GRCm39)	missense	probably benign
PIT4366001:Vmn2r59	UTSW	7	41,695,205 (GRCm39)	missense	possibly damaging	0.91
R0068:Vmn2r59	UTSW	7	41,695,725 (GRCm39)	missense	probably damaging	0.99
R0094:Vmn2r59	UTSW	7	41,661,722 (GRCm39)	missense	probably benign	0.07
R0179:Vmn2r59	UTSW	7	41,696,432 (GRCm39)	nonsense	probably null
R0370:Vmn2r59	UTSW	7	41,662,150 (GRCm39)	missense	probably benign	0.23
R0412:Vmn2r59	UTSW	7	41,695,916 (GRCm39)	splice site	probably benign
R0465:Vmn2r59	UTSW	7	41,696,332 (GRCm39)	missense	probably benign
R0487:Vmn2r59	UTSW	7	41,696,528 (GRCm39)	nonsense	probably null
R0576:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R0632:Vmn2r59	UTSW	7	41,708,308 (GRCm39)	missense	probably damaging	1.00
R1356:Vmn2r59	UTSW	7	41,661,218 (GRCm39)	makesense	probably null
R1387:Vmn2r59	UTSW	7	41,695,521 (GRCm39)	missense	probably damaging	1.00
R1388:Vmn2r59	UTSW	7	41,695,133 (GRCm39)	missense	probably benign	0.01
R1435:Vmn2r59	UTSW	7	41,695,629 (GRCm39)	missense	possibly damaging	0.50
R1750:Vmn2r59	UTSW	7	41,695,251 (GRCm39)	missense	possibly damaging	0.50
R2020:Vmn2r59	UTSW	7	41,693,203 (GRCm39)	missense	probably damaging	1.00
R2249:Vmn2r59	UTSW	7	41,708,326 (GRCm39)	missense	probably benign	0.00
R2256:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2257:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2441:Vmn2r59	UTSW	7	41,695,570 (GRCm39)	missense	probably benign	0.00
R2511:Vmn2r59	UTSW	7	41,693,190 (GRCm39)	missense	probably damaging	1.00
R2860:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R2861:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R3690:Vmn2r59	UTSW	7	41,661,370 (GRCm39)	missense	possibly damaging	0.77
R3912:Vmn2r59	UTSW	7	41,695,744 (GRCm39)	missense	probably benign	0.00
R4167:Vmn2r59	UTSW	7	41,670,732 (GRCm39)	intron	probably benign
R4357:Vmn2r59	UTSW	7	41,661,644 (GRCm39)	missense	probably damaging	1.00
R4445:Vmn2r59	UTSW	7	41,691,874 (GRCm39)	missense	probably damaging	1.00
R4542:Vmn2r59	UTSW	7	41,695,497 (GRCm39)	missense	possibly damaging	0.93
R4587:Vmn2r59	UTSW	7	41,695,648 (GRCm39)	missense	probably benign	0.00
R4616:Vmn2r59	UTSW	7	41,661,862 (GRCm39)	missense	probably benign
R4653:Vmn2r59	UTSW	7	41,693,228 (GRCm39)	missense	probably benign	0.19
R4703:Vmn2r59	UTSW	7	41,661,686 (GRCm39)	missense	probably benign	0.01
R4895:Vmn2r59	UTSW	7	41,695,218 (GRCm39)	missense	probably damaging	0.98
R4910:Vmn2r59	UTSW	7	41,693,077 (GRCm39)	missense	probably benign
R5045:Vmn2r59	UTSW	7	41,695,496 (GRCm39)	missense	possibly damaging	0.93
R5105:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R5153:Vmn2r59	UTSW	7	41,691,834 (GRCm39)	critical splice donor site	probably null
R5566:Vmn2r59	UTSW	7	41,696,247 (GRCm39)	missense	possibly damaging	0.92
R5586:Vmn2r59	UTSW	7	41,695,105 (GRCm39)	missense	probably benign	0.12
R5606:Vmn2r59	UTSW	7	41,695,318 (GRCm39)	missense	probably benign	0.27
R5616:Vmn2r59	UTSW	7	41,708,191 (GRCm39)	splice site	probably null
R5625:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R5696:Vmn2r59	UTSW	7	41,695,468 (GRCm39)	missense	probably benign	0.00
R5982:Vmn2r59	UTSW	7	41,695,491 (GRCm39)	missense	probably benign	0.00
R6106:Vmn2r59	UTSW	7	41,661,749 (GRCm39)	nonsense	probably null
R6196:Vmn2r59	UTSW	7	41,661,679 (GRCm39)	missense	probably benign	0.36
R6228:Vmn2r59	UTSW	7	41,691,835 (GRCm39)	critical splice donor site	probably null
R6590:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6625:Vmn2r59	UTSW	7	41,693,177 (GRCm39)	missense	probably benign	0.02
R6690:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6768:Vmn2r59	UTSW	7	41,661,392 (GRCm39)	missense	probably benign	0.17
R6830:Vmn2r59	UTSW	7	41,693,171 (GRCm39)	missense	probably benign	0.10
R6859:Vmn2r59	UTSW	7	41,693,277 (GRCm39)	missense	probably damaging	1.00
R7034:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7036:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7145:Vmn2r59	UTSW	7	41,695,188 (GRCm39)	missense	probably damaging	1.00
R7556:Vmn2r59	UTSW	7	41,695,233 (GRCm39)	missense	probably damaging	1.00
R7733:Vmn2r59	UTSW	7	41,661,443 (GRCm39)	missense	probably benign	0.17
R7770:Vmn2r59	UTSW	7	41,708,336 (GRCm39)	missense	probably damaging	1.00
R7812:Vmn2r59	UTSW	7	41,695,196 (GRCm39)	nonsense	probably null
R7867:Vmn2r59	UTSW	7	41,661,707 (GRCm39)	missense	probably damaging	1.00
R7999:Vmn2r59	UTSW	7	41,696,256 (GRCm39)	missense	probably damaging	1.00
R8267:Vmn2r59	UTSW	7	41,661,521 (GRCm39)	missense	probably damaging	0.97
R8367:Vmn2r59	UTSW	7	41,661,247 (GRCm39)	missense	probably benign	0.44
R9106:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R9135:Vmn2r59	UTSW	7	41,693,127 (GRCm39)	missense
R9135:Vmn2r59	UTSW	7	41,693,125 (GRCm39)	missense	probably benign	0.33
R9234:Vmn2r59	UTSW	7	41,661,907 (GRCm39)	missense	possibly damaging	0.67
R9273:Vmn2r59	UTSW	7	41,695,286 (GRCm39)	nonsense	probably null
R9432:Vmn2r59	UTSW	7	41,696,254 (GRCm39)	missense	probably damaging	1.00
R9433:Vmn2r59	UTSW	7	41,695,590 (GRCm39)	missense	probably damaging	0.99
R9616:Vmn2r59	UTSW	7	41,661,299 (GRCm39)	missense	probably damaging	1.00
R9654:Vmn2r59	UTSW	7	41,693,217 (GRCm39)	missense	probably benign	0.10
R9741:Vmn2r59	UTSW	7	41,708,209 (GRCm39)	missense	probably damaging	0.99
X0025:Vmn2r59	UTSW	7	41,695,365 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r59	UTSW	7	41,661,838 (GRCm39)	missense	possibly damaging	0.85
Z1176:Vmn2r59	UTSW	7	41,691,941 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAGAGTTCATTGATCATGACATCC -3'
(R):5'- AACAGCTGTCCTGGCTTCATG -3'

Sequencing Primer
(F):5'- ACCATAGTTTTTATGAAGGTCAAAGC -3'
(R):5'- TGGAAATGGCTGTTTCAAATGAG -3'

Posted On

2020-09-15