Mutagenetix > Incidental Mutation

Incidental Mutation 'R7975:Or10a3n'

650847

Institutional Source

Beutler Lab

Gene Symbol

Or10a3n

Ensembl Gene

ENSMUSG00000066239

Gene Name

olfactory receptor family 10 subfamily A member 3N

Synonyms

GA_x6K02T2PBJ9-11224559-11223615, MOR268-6, Olfr519

MMRRC Submission

046018-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R7975 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

108492668-108493612 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 108493019 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 203 (Y203*)

Ref Sequence

ENSEMBL: ENSMUSP00000081804 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084752] [ENSMUST00000202706]

AlphaFold

K7N645

Predicted Effect

probably null
Transcript: ENSMUST00000084752
AA Change: Y203*

SMART Domains

Protein: ENSMUSP00000081804
Gene: ENSMUSG00000066239
AA Change: Y203*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	36	313	1.3e-59	PFAM
Pfam:7TM_GPCR_Srsx	40	310	3.3e-6	PFAM
Pfam:7tm_1	46	305	3.7e-25	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000202706
AA Change: Y198*

SMART Domains

Protein: ENSMUSP00000144271
Gene: ENSMUSG00000066239
AA Change: Y198*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	4.1e-59	PFAM
Pfam:7TM_GPCR_Srsx	35	305	2.1e-5	PFAM
Pfam:7tm_1	41	300	1.2e-24	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.3%

Validation Efficiency

100% (53/53)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano4	T	C	10: 88,952,847 (GRCm39)	I60V	possibly damaging	Het
Ano5	T	A	7: 51,216,286 (GRCm39)	M381K	probably null	Het
Aox1	T	A	1: 58,348,187 (GRCm39)	I635K	probably benign	Het
Bdp1	C	T	13: 100,156,884 (GRCm39)	C2436Y	probably benign	Het
Bod1l	T	C	5: 41,973,620 (GRCm39)	R2565G	possibly damaging	Het
Brd2	A	T	17: 34,334,424 (GRCm39)	I245N	probably damaging	Het
Capn9	G	A	8: 125,325,515 (GRCm39)	V258M	probably damaging	Het
Ccar2	C	G	14: 70,380,918 (GRCm39)	C324S	possibly damaging	Het
Cd209b	A	T	8: 3,975,948 (GRCm39)	I71N	probably benign	Het
Cdk12	A	T	11: 98,111,928 (GRCm39)	I729F	unknown	Het
Celf1	T	G	2: 90,831,423 (GRCm39)	V84G	probably damaging	Het
Cgn	G	T	3: 94,671,836 (GRCm39)	A965E	probably benign	Het
Cgnl1	T	C	9: 71,632,604 (GRCm39)	E249G	probably benign	Het
Cspg4b	A	G	13: 113,455,841 (GRCm39)	H629R		Het
Cyp2j8	T	C	4: 96,358,776 (GRCm39)	N381S	possibly damaging	Het
Dmtf1	T	C	5: 9,179,169 (GRCm39)	D343G	probably damaging	Het
Fhl5	T	A	4: 25,214,730 (GRCm39)	I16F	probably benign	Het
Gatd1	A	G	7: 140,989,781 (GRCm39)	F143S	probably damaging	Het
Gbp10	C	T	5: 105,368,967 (GRCm39)	G291R	probably benign	Het
Gm28168	T	C	1: 117,875,820 (GRCm39)	Y150H	probably benign	Het
Gm3543	T	C	14: 41,802,122 (GRCm39)	M121V	probably benign	Het
Gramd2b	A	G	18: 56,618,451 (GRCm39)	T220A	probably benign	Het
Gstcd	A	G	3: 132,777,863 (GRCm39)	L316P	probably damaging	Het
Hspg2	G	A	4: 137,282,532 (GRCm39)	G3424D	probably benign	Het
Ifi209	C	T	1: 173,468,722 (GRCm39)	S184F	probably benign	Het
Ino80	A	T	2: 119,286,948 (GRCm39)		probably null	Het
Kcnd3	G	A	3: 105,366,310 (GRCm39)	R60H	probably damaging	Het
Kif21b	T	C	1: 136,098,911 (GRCm39)	C1400R	probably damaging	Het
Lama3	C	T	18: 12,670,796 (GRCm39)	P794L	probably damaging	Het
Lgals4	A	G	7: 28,540,346 (GRCm39)	I214V	probably benign	Het
Lipo4	A	T	19: 33,490,028 (GRCm39)	L158Q	probably damaging	Het
Ltv1	A	G	10: 13,066,453 (GRCm39)	Y58H	probably damaging	Het
Mitf	T	C	6: 97,994,990 (GRCm39)	S479P	probably benign	Het
Mthfr	A	T	4: 148,127,920 (GRCm39)	D132V	probably damaging	Het
Mtrr	T	C	13: 68,727,666 (GRCm39)		probably null	Het
Neb	T	A	2: 52,050,678 (GRCm39)	H6660L	probably benign	Het
Nhlrc3	A	T	3: 53,360,966 (GRCm39)	V263E	probably damaging	Het
Oas1h	G	A	5: 121,009,890 (GRCm39)	V322M	probably damaging	Het
Or2m13	T	A	16: 19,226,301 (GRCm39)	D155V	probably damaging	Het
Or4a15	C	T	2: 89,193,413 (GRCm39)	R120H	probably benign	Het
Pappa	A	T	4: 65,212,705 (GRCm39)	D1121V	probably damaging	Het
Pax5	A	G	4: 44,537,465 (GRCm39)	S352P	probably damaging	Het
Piezo1	C	A	8: 123,222,504 (GRCm39)	R915L		Het
R3hcc1	T	C	14: 69,944,593 (GRCm39)	D7G	probably damaging	Het
Rasgrp2	A	G	19: 6,458,589 (GRCm39)	K429E	probably damaging	Het
Scn10a	A	G	9: 119,501,286 (GRCm39)	F166S	probably benign	Het
Scn9a	T	G	2: 66,314,597 (GRCm39)	N1707T	probably damaging	Het
Sec23ip	T	C	7: 128,364,201 (GRCm39)	F493S	probably damaging	Het
Slc1a7	G	A	4: 107,869,473 (GRCm39)	V513M	probably benign	Het
Slfn4	A	T	11: 83,077,982 (GRCm39)	I257F	possibly damaging	Het
Smarcal1	T	A	1: 72,652,150 (GRCm39)	D637E	probably benign	Het
Smpd3	A	C	8: 106,982,254 (GRCm39)	C617G	probably benign	Het
Sos2	A	T	12: 69,639,814 (GRCm39)	F990L	probably benign	Het
Spata31h1	C	T	10: 82,119,823 (GRCm39)	A4396T	possibly damaging	Het
Syne1	T	C	10: 4,981,786 (GRCm39)	T844A	probably benign	Het
Tph1	G	A	7: 46,306,678 (GRCm39)	R145C	probably damaging	Het
Ubap2l	G	A	3: 89,946,076 (GRCm39)		probably null	Het
Vmn2r50	T	C	7: 9,771,272 (GRCm39)	I810V	probably benign	Het
Vmn2r59	T	C	7: 41,693,199 (GRCm39)	D467G	probably damaging	Het
Zfp618	T	A	4: 63,049,352 (GRCm39)	N417K	possibly damaging	Het

Other mutations in Or10a3n

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01634:Or10a3n	APN	7	108,493,292 (GRCm39)	missense	probably benign	0.22
IGL02043:Or10a3n	APN	7	108,493,046 (GRCm39)	nonsense	probably null
PIT4382001:Or10a3n	UTSW	7	108,493,309 (GRCm39)	missense	probably damaging	1.00
R1183:Or10a3n	UTSW	7	108,492,948 (GRCm39)	missense	probably damaging	1.00
R1596:Or10a3n	UTSW	7	108,493,086 (GRCm39)	missense	probably damaging	1.00
R1647:Or10a3n	UTSW	7	108,492,972 (GRCm39)	missense	probably damaging	1.00
R1648:Or10a3n	UTSW	7	108,492,972 (GRCm39)	missense	probably damaging	1.00
R3952:Or10a3n	UTSW	7	108,493,189 (GRCm39)	missense	probably benign	0.00
R4611:Or10a3n	UTSW	7	108,493,324 (GRCm39)	missense	probably damaging	1.00
R4723:Or10a3n	UTSW	7	108,493,028 (GRCm39)	missense	probably benign	0.09
R5838:Or10a3n	UTSW	7	108,493,292 (GRCm39)	missense	probably benign	0.22
R6483:Or10a3n	UTSW	7	108,493,318 (GRCm39)	missense	possibly damaging	0.76
R6516:Or10a3n	UTSW	7	108,492,972 (GRCm39)	missense	probably damaging	1.00
R7353:Or10a3n	UTSW	7	108,493,429 (GRCm39)	missense	probably damaging	1.00
R7748:Or10a3n	UTSW	7	108,493,285 (GRCm39)	missense	probably benign	0.22
R8823:Or10a3n	UTSW	7	108,493,155 (GRCm39)	missense	probably benign	0.00
R8913:Or10a3n	UTSW	7	108,492,736 (GRCm39)	missense	probably damaging	0.99
R8914:Or10a3n	UTSW	7	108,492,736 (GRCm39)	missense	probably damaging	0.99
R9066:Or10a3n	UTSW	7	108,493,253 (GRCm39)	nonsense	probably null
R9093:Or10a3n	UTSW	7	108,493,609 (GRCm39)	missense	probably benign	0.05
R9763:Or10a3n	UTSW	7	108,493,210 (GRCm39)	missense	probably benign	0.01
Z1088:Or10a3n	UTSW	7	108,492,980 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CTGTGCCATAGAAGAGGATGAC -3'
(R):5'- GGGGCAATGGCTTATGATCG -3'

Sequencing Primer
(F):5'- CAGAGGTGAGATGAGAGGCACAC -3'
(R):5'- TGACCTACCCAGTGATTATGAGC -3'

Posted On

2020-09-15