Mutagenetix > Incidental Mutation

Incidental Mutation 'R7977:Or6c66'

650977

Institutional Source

Beutler Lab

Gene Symbol

Or6c66

Ensembl Gene

ENSMUSG00000095002

Gene Name

olfactory receptor family 6 subfamily C member 66

Synonyms

GA_x6K02T2PULF-11304679-11303744, MOR108-1, Olfr798

MMRRC Submission

046020-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.191) question?

Stock #

R7977 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

129460993-129461928 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 129461838 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 31 (I31V)

Ref Sequence

ENSEMBL: ENSMUSP00000144716 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079810] [ENSMUST00000204979]

AlphaFold

Q7TRH8

Predicted Effect

probably benign
Transcript: ENSMUST00000079810
AA Change: I31V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000078739
Gene: ENSMUSG00000095002
AA Change: I31V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	309	5e-52	PFAM
Pfam:7tm_1	39	288	2.1e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000204979
AA Change: I31V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000144716
Gene: ENSMUSG00000095002
AA Change: I31V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	309	5e-52	PFAM
Pfam:7tm_1	39	288	2.1e-25	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9430097D07Rik	G	T	2: 32,464,317 (GRCm39)	Q161K	unknown	Het
Acsl5	T	C	19: 55,266,405 (GRCm39)		probably null	Het
Adgre1	T	A	17: 57,754,987 (GRCm39)	I695N	possibly damaging	Het
Ank2	T	C	3: 126,739,356 (GRCm39)	D2176G	unknown	Het
Atp6v0a2	G	A	5: 124,797,050 (GRCm39)	G810D	probably damaging	Het
Bod1l	A	C	5: 41,952,413 (GRCm39)	N2868K	probably damaging	Het
Brd7	T	C	8: 89,060,769 (GRCm39)	T526A	probably benign	Het
C9	T	A	15: 6,496,943 (GRCm39)	Y213*	probably null	Het
Card6	T	A	15: 5,130,007 (GRCm39)	N463I	probably damaging	Het
Ccdc81	T	C	7: 89,525,319 (GRCm39)	Y485C	probably damaging	Het
Celsr1	A	G	15: 85,917,194 (GRCm39)	F260L	probably damaging	Het
Dnah8	A	G	17: 30,963,498 (GRCm39)	D2304G	probably damaging	Het
Dnase1	A	G	16: 3,855,834 (GRCm39)	D55G	probably damaging	Het
Ecsit	C	A	9: 21,984,780 (GRCm39)	R296L	probably damaging	Het
Entpd8	A	G	2: 24,974,778 (GRCm39)	D403G	probably damaging	Het
Epha2	C	A	4: 141,035,791 (GRCm39)	Q76K	probably damaging	Het
Exoc1	T	A	5: 76,691,432 (GRCm39)	V252E	probably damaging	Het
Fmnl3	T	A	15: 99,225,979 (GRCm39)	H225L	possibly damaging	Het
Gm4565	A	C	7: 22,282,812 (GRCm39)	M2R	probably benign	Het
Gpr146	G	T	5: 139,378,440 (GRCm39)	A81S	possibly damaging	Het
Heg1	C	A	16: 33,541,100 (GRCm39)	S419*	probably null	Het
Hps5	A	C	7: 46,418,475 (GRCm39)	I865S	probably benign	Het
Hsp90ab1	A	C	17: 45,882,532 (GRCm39)	I54S	probably damaging	Het
Hyal4	T	G	6: 24,763,865 (GRCm39)	M342R	probably damaging	Het
Itgal	A	G	7: 126,927,470 (GRCm39)	T987A	possibly damaging	Het
Kat6b	A	G	14: 21,719,931 (GRCm39)	T1428A	probably benign	Het
Krt9	TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC	TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC	11: 100,079,903 (GRCm39)		probably benign	Het
Ldlrad4	G	T	18: 68,368,740 (GRCm39)	A66S	possibly damaging	Het
Lmtk2	A	G	5: 144,111,959 (GRCm39)	D893G	probably benign	Het
Myo3b	A	G	2: 70,161,277 (GRCm39)	T1174A	probably benign	Het
Naf1	CGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGA	CGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGA	8: 67,313,146 (GRCm39)		probably benign	Het
Nsun5	G	A	5: 135,404,534 (GRCm39)	R424H	probably damaging	Het
Oacyl	A	G	18: 65,831,462 (GRCm39)	E33G	probably benign	Het
Or7d10	T	C	9: 19,831,610 (GRCm39)	F35S	possibly damaging	Het
Palm	C	T	10: 79,629,539 (GRCm39)		probably benign	Het
Papln	A	G	12: 83,822,156 (GRCm39)	E337G	probably damaging	Het
Pira2	A	T	7: 3,844,696 (GRCm39)	F445Y	probably benign	Het
Setd1b	A	G	5: 123,285,743 (GRCm39)	D263G	unknown	Het
Slc10a7	T	A	8: 79,423,843 (GRCm39)	F202I	probably benign	Het
Smpd3	T	C	8: 106,986,526 (GRCm39)	I455V	probably benign	Het
Snip1	G	T	4: 124,960,732 (GRCm39)	G63W	probably damaging	Het
Sorl1	T	A	9: 41,888,857 (GRCm39)	Y1981F	probably damaging	Het
Tagln2	A	G	1: 172,332,820 (GRCm39)	T36A	probably benign	Het
Tnxb	T	C	17: 34,929,194 (GRCm39)	S2746P	possibly damaging	Het
Tob2	G	A	15: 81,735,681 (GRCm39)	P96L	probably damaging	Het
Triobp	T	A	15: 78,885,744 (GRCm39)	Y1816N	probably damaging	Het
Trpc3	A	G	3: 36,698,318 (GRCm39)	I647T	probably benign	Het
Ttn	A	G	2: 76,721,905 (GRCm39)	S6600P	unknown	Het
Vmn1r200	A	T	13: 22,580,025 (GRCm39)	Y276F	possibly damaging	Het
Vmn1r230	T	C	17: 21,067,159 (GRCm39)	I116T	probably benign	Het
Vmn1r26	A	T	6: 57,985,264 (GRCm39)	Y308*	probably null	Het
Vmn2r16	C	T	5: 109,488,015 (GRCm39)	T296I	probably damaging	Het
Wdfy2	A	G	14: 63,189,380 (GRCm39)	D283G	possibly damaging	Het

Other mutations in Or6c66

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Or6c66	APN	10	129,461,432 (GRCm39)	missense	probably damaging	1.00
IGL02866:Or6c66	APN	10	129,461,575 (GRCm39)	nonsense	probably null
BB009:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
BB019:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
R0743:Or6c66	UTSW	10	129,461,712 (GRCm39)	missense	probably benign	0.03
R1163:Or6c66	UTSW	10	129,461,516 (GRCm39)	missense	possibly damaging	0.79
R1192:Or6c66	UTSW	10	129,461,906 (GRCm39)	missense	probably benign
R1863:Or6c66	UTSW	10	129,461,217 (GRCm39)	missense	probably damaging	0.96
R1867:Or6c66	UTSW	10	129,461,621 (GRCm39)	missense	probably damaging	0.99
R2508:Or6c66	UTSW	10	129,461,784 (GRCm39)	missense	probably benign
R4898:Or6c66	UTSW	10	129,461,468 (GRCm39)	missense	probably benign	0.12
R4910:Or6c66	UTSW	10	129,461,676 (GRCm39)	missense	probably damaging	0.98
R4988:Or6c66	UTSW	10	129,461,930 (GRCm39)	splice site	probably null
R5361:Or6c66	UTSW	10	129,461,601 (GRCm39)	missense	probably damaging	1.00
R5382:Or6c66	UTSW	10	129,461,876 (GRCm39)	missense	probably damaging	0.98
R5589:Or6c66	UTSW	10	129,461,319 (GRCm39)	missense	probably damaging	1.00
R5693:Or6c66	UTSW	10	129,461,396 (GRCm39)	missense	probably damaging	0.99
R5790:Or6c66	UTSW	10	129,461,757 (GRCm39)	missense	probably damaging	1.00
R6966:Or6c66	UTSW	10	129,461,633 (GRCm39)	missense	probably benign	0.17
R7041:Or6c66	UTSW	10	129,461,603 (GRCm39)	missense	probably damaging	1.00
R7082:Or6c66	UTSW	10	129,461,634 (GRCm39)	missense	probably benign	0.07
R7932:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
R7987:Or6c66	UTSW	10	129,461,838 (GRCm39)	missense	probably benign
R8299:Or6c66	UTSW	10	129,461,829 (GRCm39)	missense	probably benign	0.00
R8487:Or6c66	UTSW	10	129,461,114 (GRCm39)	missense	possibly damaging	0.50
R9110:Or6c66	UTSW	10	129,461,820 (GRCm39)	missense	possibly damaging	0.88

Predicted Primers

PCR Primer
(F):5'- CCTGTCACAATGCTCACAAG -3'
(R):5'- CCAGATGCATAGGTGTTTTGTGAC -3'

Sequencing Primer
(F):5'- TGTCACAATGCTCACAAGGAAGC -3'
(R):5'- GGTGTGATGAAACAAACACATATACC -3'

Posted On

2020-09-15