Mutagenetix > Incidental Mutation

Incidental Mutation 'R7980:Ubr4'

651129

Institutional Source

Beutler Lab

Gene Symbol

Ubr4

Ensembl Gene

ENSMUSG00000066036

Gene Name

ubiquitin protein ligase E3 component n-recognin 4

Synonyms

LOC381562, D930005K06Rik, Zubr1, p600, 1810009A16Rik, A930005E13Rik

MMRRC Submission

046021-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7980 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

139352609-139489588 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 139418406 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Phenylalanine at position 216 (V216F)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000097822] [ENSMUST00000147999] [ENSMUST00000165860]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000097822
AA Change: V1505F

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000095433
Gene: ENSMUSG00000066036
AA Change: V1505F

Domain	Start	End	E-Value	Type
low complexity region	5	20	N/A	INTRINSIC
low complexity region	414	425	N/A	INTRINSIC
low complexity region	530	541	N/A	INTRINSIC
low complexity region	555	566	N/A	INTRINSIC
low complexity region	571	588	N/A	INTRINSIC
low complexity region	600	621	N/A	INTRINSIC
low complexity region	1312	1330	N/A	INTRINSIC
low complexity region	1642	1655	N/A	INTRINSIC
Pfam:zf-UBR	1660	1725	1.9e-9	PFAM
Blast:ZnF_C2H2	1966	1991	6e-8	BLAST
low complexity region	2268	2279	N/A	INTRINSIC
low complexity region	2502	2540	N/A	INTRINSIC
low complexity region	2725	2735	N/A	INTRINSIC
low complexity region	2818	2852	N/A	INTRINSIC
low complexity region	2887	2905	N/A	INTRINSIC
low complexity region	2928	2942	N/A	INTRINSIC
low complexity region	2945	2959	N/A	INTRINSIC
low complexity region	2966	2986	N/A	INTRINSIC
low complexity region	3063	3091	N/A	INTRINSIC
low complexity region	3329	3385	N/A	INTRINSIC
low complexity region	3776	3788	N/A	INTRINSIC
Pfam:E3_UbLigase_R4	4364	5160	N/A	PFAM

Predicted Effect

SMART Domains

Protein: ENSMUSP00000115711
Gene: ENSMUSG00000066036
AA Change: V216F

Domain	Start	End	E-Value	Type
low complexity region	24	42	N/A	INTRINSIC
low complexity region	354	367	N/A	INTRINSIC
Pfam:zf-UBR	372	437	2.1e-10	PFAM
Blast:ZnF_C2H2	678	703	5e-8	BLAST
low complexity region	980	991	N/A	INTRINSIC
low complexity region	1225	1263	N/A	INTRINSIC
low complexity region	1448	1458	N/A	INTRINSIC
low complexity region	1575	1593	N/A	INTRINSIC
low complexity region	1616	1630	N/A	INTRINSIC
low complexity region	1633	1647	N/A	INTRINSIC
low complexity region	1654	1674	N/A	INTRINSIC
low complexity region	1751	1779	N/A	INTRINSIC
low complexity region	2017	2045	N/A	INTRINSIC
low complexity region	2048	2065	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000147999

SMART Domains

Protein: ENSMUSP00000117419
Gene: ENSMUSG00000066036

Domain	Start	End	E-Value	Type
low complexity region	170	226	N/A	INTRINSIC
low complexity region	617	629	N/A	INTRINSIC
Pfam:E3_UbLigase_R4	1205	1301	4.5e-60	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000165860
AA Change: V1505F

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000125800
Gene: ENSMUSG00000066036
AA Change: V1505F

Domain	Start	End	E-Value	Type
low complexity region	5	20	N/A	INTRINSIC
low complexity region	414	425	N/A	INTRINSIC
low complexity region	530	541	N/A	INTRINSIC
low complexity region	555	566	N/A	INTRINSIC
low complexity region	571	588	N/A	INTRINSIC
low complexity region	600	621	N/A	INTRINSIC
low complexity region	1312	1330	N/A	INTRINSIC
low complexity region	1642	1655	N/A	INTRINSIC
Pfam:zf-UBR	1659	1729	4e-13	PFAM
Blast:ZnF_C2H2	1966	1991	5e-8	BLAST
low complexity region	2268	2279	N/A	INTRINSIC
low complexity region	2513	2551	N/A	INTRINSIC
low complexity region	2736	2746	N/A	INTRINSIC
low complexity region	2863	2881	N/A	INTRINSIC
low complexity region	2904	2918	N/A	INTRINSIC
low complexity region	2921	2935	N/A	INTRINSIC
low complexity region	2942	2962	N/A	INTRINSIC
low complexity region	3039	3067	N/A	INTRINSIC
low complexity region	3305	3361	N/A	INTRINSIC
low complexity region	3752	3764	N/A	INTRINSIC
Pfam:E3_UbLigase_R4	4340	5136	N/A	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (87/87)

MGI Phenotype

Strain: 5286698
Lethality: D1-D5
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit neonatal lethality and decreased body size at birth. Mice homozygous for a null mutation display complete embryonic lethality during organogenesis with arrest of vitelline vascular remodeling. [provided by MGI curators]

Allele List at MGI

All alleles(59) : Targeted(2) Gene trapped(56) Transgenic(1)

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930553M12Rik	A	G	4: 88,868,078 (GRCm38)	L101P	unknown	Het
Actn3	G	A	19: 4,867,922 (GRCm38)	P339L	probably damaging	Het
Adamts12	G	A	15: 11,263,337 (GRCm38)	C595Y	probably damaging	Het
Adrm1b	T	A	3: 92,429,099 (GRCm38)	K99*	probably null	Het
Agl	A	T	3: 116,792,181 (GRCm38)	N99K	probably benign	Het
Aplp1	A	T	7: 30,435,567 (GRCm38)	M592K	probably benign	Het
Arhgef11	C	T	3: 87,697,990 (GRCm38)	R251C	probably benign	Het
Arhgef12	A	T	9: 42,971,299 (GRCm38)	C1416*	probably null	Het
Asah1	A	G	8: 41,354,030 (GRCm38)	M119T		Het
Asxl2	A	T	12: 3,496,630 (GRCm38)	Q471H	probably damaging	Het
Atp6v1c2	A	T	12: 17,321,612 (GRCm38)	D61E	probably damaging	Het
Btbd16	C	A	7: 130,824,367 (GRCm38)	P520Q	probably damaging	Het
Cacnb2	A	G	2: 14,604,515 (GRCm38)	E22G	probably benign	Het
Catspere2	G	T	1: 178,003,044 (GRCm38)		probably null	Het
Cdc25a	T	A	9: 109,879,881 (GRCm38)	D124E	probably damaging	Het
Cdc42se1	A	T	3: 95,231,855 (GRCm38)		probably benign	Het
Cdcp3	T	A	7: 131,234,777 (GRCm38)	V400E	probably damaging	Het
Cfap54	T	C	10: 92,982,060 (GRCm38)	K1265E	possibly damaging	Het
Chaf1b	A	G	16: 93,884,527 (GRCm38)	H11R	probably damaging	Het
Chil4	T	C	3: 106,202,744 (GRCm38)	K345E	probably damaging	Het
Cntnap1	A	G	11: 101,188,893 (GRCm38)	I1283V	probably benign	Het
Dagla	C	T	19: 10,252,042 (GRCm38)	C618Y	possibly damaging	Het
Ddx19b	C	T	8: 111,011,445 (GRCm38)	V224M	possibly damaging	Het
Dohh	C	T	10: 81,387,892 (GRCm38)	R260*	probably null	Het
Dsg3	A	T	18: 20,531,360 (GRCm38)	N472Y	probably benign	Het
Duox1	T	A	2: 122,347,320 (GRCm38)	N1528K	possibly damaging	Het
Eml6	A	G	11: 29,833,205 (GRCm38)	Y559H	probably damaging	Het
Esp4	A	G	17: 40,602,301 (GRCm38)	T20A	possibly damaging	Het
Fbxw21	T	C	9: 109,156,571 (GRCm38)		probably null	Het
Gcc2	T	A	10: 58,278,752 (GRCm38)		probably null	Het
Grin3b	C	T	10: 79,975,725 (GRCm38)	A715V	possibly damaging	Het
Gxylt2	A	T	6: 100,787,209 (GRCm38)		probably null	Het
Hspa4	A	T	11: 53,280,577 (GRCm38)	S267T	probably benign	Het
Hspd1	A	G	1: 55,078,626 (GRCm38)	V491A	possibly damaging	Het
Idua	A	G	5: 108,680,620 (GRCm38)	E280G	probably benign	Het
Ift172	T	A	5: 31,260,644 (GRCm38)	E1267V	probably benign	Het
Itsn1	T	A	16: 91,905,294 (GRCm38)	V1448E	unknown	Het
Kat6a	A	G	8: 22,926,416 (GRCm38)	M647V	possibly damaging	Het
Kcnq4	A	T	4: 120,711,297 (GRCm38)	D407E	probably benign	Het
Kif1b	A	T	4: 149,269,921 (GRCm38)	F221I	probably damaging	Het
Lama2	G	T	10: 27,363,613 (GRCm38)	D315E	probably damaging	Het
Lrrn4	G	A	2: 132,878,176 (GRCm38)	L235F	probably damaging	Het
Map2k6	T	A	11: 110,499,384 (GRCm38)	I248N		Het
Mgat5	A	G	1: 127,479,511 (GRCm38)	Q638R	probably benign	Het
Mia2	A	G	12: 59,108,865 (GRCm38)	K455E	probably damaging	Het
Mrs2	A	G	13: 25,020,238 (GRCm38)	C3R	possibly damaging	Het
Nprl3	A	G	11: 32,237,357 (GRCm38)	I325T	probably damaging	Het
Nr1h3	T	A	2: 91,190,884 (GRCm38)	Q186L	probably benign	Het
Oas1f	A	G	5: 120,851,475 (GRCm38)	E159G	probably benign	Het
Or2g7	A	T	17: 38,067,521 (GRCm38)	M117L	possibly damaging	Het
Or4k15b	T	A	14: 50,034,795 (GRCm38)	S203C	probably damaging	Het
Or52ae7	T	A	7: 103,470,297 (GRCm38)	F86Y	probably damaging	Het
Or52e19b	T	A	7: 103,383,763 (GRCm38)	I80F	probably damaging	Het
Or52s1b	T	A	7: 103,173,035 (GRCm38)	S201C	probably damaging	Het
Or5m12	A	G	2: 85,904,598 (GRCm38)	F152S	probably benign	Het
Or8g4	T	C	9: 39,751,121 (GRCm38)	I245T	probably damaging	Het
Or9a4	T	A	6: 40,572,220 (GRCm38)	I278N	probably benign	Het
Pbxip1	G	C	3: 89,446,341 (GRCm38)	S267T	probably benign	Het
Pcdhga10	A	T	18: 37,748,592 (GRCm38)	I469F	possibly damaging	Het
Pkd1l1	A	G	11: 8,854,375 (GRCm38)	S1739P	probably damaging	Het
Plcd4	A	C	1: 74,565,305 (GRCm38)	N788T	probably benign	Het
Pms2	T	A	5: 143,931,091 (GRCm38)	W838R	probably damaging	Het
Pnpla6	T	A	8: 3,536,562 (GRCm38)	V926D	probably damaging	Het
Pros1	A	T	16: 62,928,153 (GRCm38)	H663L	possibly damaging	Het
Prss55	T	C	14: 64,078,689 (GRCm38)		probably null	Het
Pum2	T	C	12: 8,713,904 (GRCm38)	Y283H	probably damaging	Het
Rad21	A	G	15: 51,965,026 (GRCm38)	S549P	probably benign	Het
Sap130	T	A	18: 31,648,129 (GRCm38)		probably null	Het
Sertad4	C	T	1: 192,846,881 (GRCm38)	S209N	probably benign	Het
Sez6	A	G	11: 77,953,842 (GRCm38)	T164A	probably benign	Het
Slc22a28	C	T	19: 8,101,473 (GRCm38)	R284Q	probably damaging	Het
Slco4c1	T	C	1: 96,836,925 (GRCm38)	K474R	probably benign	Het
Stx5a	T	C	19: 8,742,438 (GRCm38)	S56P	probably damaging	Het
Svopl	T	C	6: 38,014,809 (GRCm38)	T379A	probably damaging	Het
Sypl2	A	G	3: 108,217,692 (GRCm38)	F118L	probably damaging	Het
Tfrc	G	A	16: 32,617,149 (GRCm38)	V215M	probably benign	Het
Thnsl2	T	C	6: 71,138,668 (GRCm38)	N185S	probably damaging	Het
Tmem104	T	C	11: 115,243,754 (GRCm38)	I372T	probably damaging	Het
Tnfaip6	G	A	2: 52,051,058 (GRCm38)	G204S	probably damaging	Het
Trim12a	C	T	7: 104,304,128 (GRCm38)	E259K	probably benign	Het
Tubgcp6	G	A	15: 89,102,029 (GRCm38)	R1436C	probably benign	Het
Tyrp1	C	T	4: 80,840,627 (GRCm38)	L246F	probably damaging	Het
Unc5a	A	G	13: 54,999,506 (GRCm38)	I409V	possibly damaging	Het
Vmn1r15	T	C	6: 57,258,414 (GRCm38)	L89P	probably damaging	Het
Vmn1r32	A	T	6: 66,553,321 (GRCm38)	L157*	probably null	Het
Zcchc24	A	G	14: 25,719,761 (GRCm38)	Y160H	probably damaging	Het
Zfp184	A	T	13: 21,960,206 (GRCm38)	H694L	probably damaging	Het
Zfp345	A	C	2: 150,472,803 (GRCm38)	Y271*	probably null	Het
Zranb3	T	C	1: 128,102,934 (GRCm38)		probably benign	Het

Other mutations in Ubr4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Ubr4	APN	4	139,465,322 (GRCm38)	missense	possibly damaging	0.46
IGL00336:Ubr4	APN	4	139,428,566 (GRCm38)	missense	probably damaging	1.00
IGL00586:Ubr4	APN	4	139,455,184 (GRCm38)	missense	possibly damaging	0.95
IGL00659:Ubr4	APN	4	139,421,245 (GRCm38)	missense	probably damaging	1.00
IGL00766:Ubr4	APN	4	139,440,766 (GRCm38)	missense	probably damaging	1.00
IGL00819:Ubr4	APN	4	139,476,282 (GRCm38)	missense	possibly damaging	0.92
IGL00833:Ubr4	APN	4	139,393,159 (GRCm38)	critical splice donor site	probably null
IGL01126:Ubr4	APN	4	139,402,555 (GRCm38)	missense	probably benign	0.04
IGL01311:Ubr4	APN	4	139,479,045 (GRCm38)	missense	possibly damaging	0.66
IGL01349:Ubr4	APN	4	139,480,728 (GRCm38)	missense	unknown
IGL01388:Ubr4	APN	4	139,460,243 (GRCm38)	missense	possibly damaging	0.76
IGL01417:Ubr4	APN	4	139,410,800 (GRCm38)	missense	probably damaging	0.99
IGL01446:Ubr4	APN	4	139,438,040 (GRCm38)	splice site	probably benign
IGL01449:Ubr4	APN	4	139,412,736 (GRCm38)	missense	probably damaging	0.98
IGL01545:Ubr4	APN	4	139,442,829 (GRCm38)	splice site	probably benign
IGL01568:Ubr4	APN	4	139,421,373 (GRCm38)	missense	probably damaging	1.00
IGL01596:Ubr4	APN	4	139,462,534 (GRCm38)	splice site	probably benign
IGL01621:Ubr4	APN	4	139,440,783 (GRCm38)	nonsense	probably null
IGL01639:Ubr4	APN	4	139,417,344 (GRCm38)	missense	probably damaging	0.99
IGL01655:Ubr4	APN	4	139,407,796 (GRCm38)	missense	probably damaging	1.00
IGL01710:Ubr4	APN	4	139,418,461 (GRCm38)	missense	possibly damaging	0.81
IGL01830:Ubr4	APN	4	139,472,500 (GRCm38)	missense	probably damaging	0.99
IGL01862:Ubr4	APN	4	139,477,158 (GRCm38)	missense	possibly damaging	0.66
IGL01868:Ubr4	APN	4	139,412,678 (GRCm38)	nonsense	probably null
IGL01874:Ubr4	APN	4	139,393,289 (GRCm38)	splice site	probably benign
IGL01889:Ubr4	APN	4	139,462,472 (GRCm38)	nonsense	probably null
IGL01891:Ubr4	APN	4	139,436,260 (GRCm38)	critical splice donor site	probably null
IGL01980:Ubr4	APN	4	139,429,602 (GRCm38)	missense	probably damaging	0.99
IGL02126:Ubr4	APN	4	139,452,741 (GRCm38)	critical splice donor site	probably null
IGL02153:Ubr4	APN	4	139,460,160 (GRCm38)	nonsense	probably null
IGL02173:Ubr4	APN	4	139,437,070 (GRCm38)	splice site	probably null
IGL02214:Ubr4	APN	4	139,428,583 (GRCm38)	missense	possibly damaging	0.95
IGL02214:Ubr4	APN	4	139,461,827 (GRCm38)	critical splice acceptor site	probably null
IGL02220:Ubr4	APN	4	139,388,435 (GRCm38)	missense	probably benign	0.01
IGL02246:Ubr4	APN	4	139,459,103 (GRCm38)	missense	possibly damaging	0.89
IGL02264:Ubr4	APN	4	139,455,628 (GRCm38)	nonsense	probably null
IGL02273:Ubr4	APN	4	139,472,578 (GRCm38)	missense	possibly damaging	0.94
IGL02316:Ubr4	APN	4	139,473,178 (GRCm38)	missense	possibly damaging	0.46
IGL02328:Ubr4	APN	4	139,478,922 (GRCm38)	missense	probably damaging	0.97
IGL02476:Ubr4	APN	4	139,421,249 (GRCm38)	nonsense	probably null
IGL02477:Ubr4	APN	4	139,436,205 (GRCm38)	missense	probably damaging	0.98
IGL02544:Ubr4	APN	4	139,415,118 (GRCm38)	missense	probably damaging	1.00
IGL02622:Ubr4	APN	4	139,467,250 (GRCm38)	nonsense	probably null
IGL02679:Ubr4	APN	4	139,459,134 (GRCm38)	missense	probably damaging	0.99
IGL02728:Ubr4	APN	4	139,468,811 (GRCm38)	missense	probably damaging	1.00
IGL02754:Ubr4	APN	4	139,410,784 (GRCm38)	missense	probably damaging	0.99
IGL02754:Ubr4	APN	4	139,393,159 (GRCm38)	critical splice donor site	probably null
IGL02892:Ubr4	APN	4	139,417,331 (GRCm38)	missense	probably damaging	0.96
IGL02897:Ubr4	APN	4	139,472,508 (GRCm38)	missense	probably damaging	0.97
IGL02946:Ubr4	APN	4	139,425,295 (GRCm38)	missense	probably damaging	1.00
IGL02964:Ubr4	APN	4	139,407,820 (GRCm38)	missense	possibly damaging	0.84
IGL03059:Ubr4	APN	4	139,480,676 (GRCm38)	missense	probably damaging	0.98
IGL03068:Ubr4	APN	4	139,409,730 (GRCm38)	missense	probably benign	0.02
IGL03087:Ubr4	APN	4	139,450,357 (GRCm38)	nonsense	probably null
IGL03116:Ubr4	APN	4	139,479,581 (GRCm38)	splice site	probably benign
IGL03212:Ubr4	APN	4	139,409,763 (GRCm38)	missense	probably benign	0.13
IGL03228:Ubr4	APN	4	139,429,598 (GRCm38)	missense	probably damaging	1.00
IGL03292:Ubr4	APN	4	139,440,435 (GRCm38)	missense	probably damaging	1.00
IGL03388:Ubr4	APN	4	139,415,032 (GRCm38)	missense	probably damaging	0.98
IGL03393:Ubr4	APN	4	139,452,678 (GRCm38)	missense	probably damaging	1.00
IGL03409:Ubr4	APN	4	139,399,929 (GRCm38)	nonsense	probably null
Aqua_regia	UTSW	4	139,452,719 (GRCm38)	nonsense	probably null
Eats	UTSW	4	139,463,575 (GRCm38)	missense	probably damaging	0.97
Hardened	UTSW	4	139,468,847 (GRCm38)	missense	probably damaging	1.00
Stoney	UTSW	4	139,444,687 (GRCm38)	missense	probably damaging	1.00
Uber	UTSW	4	139,479,062 (GRCm38)	critical splice donor site	probably null
BB001:Ubr4	UTSW	4	139,467,276 (GRCm38)	missense	unknown
BB011:Ubr4	UTSW	4	139,467,276 (GRCm38)	missense	unknown
P0019:Ubr4	UTSW	4	139,451,781 (GRCm38)	missense	probably damaging	1.00
PIT4544001:Ubr4	UTSW	4	139,402,560 (GRCm38)	missense	possibly damaging	0.93
R0001:Ubr4	UTSW	4	139,451,788 (GRCm38)	missense	probably damaging	1.00
R0002:Ubr4	UTSW	4	139,390,900 (GRCm38)	missense	probably damaging	1.00
R0006:Ubr4	UTSW	4	139,431,649 (GRCm38)	missense	probably benign	0.03
R0006:Ubr4	UTSW	4	139,431,649 (GRCm38)	missense	probably benign	0.03
R0008:Ubr4	UTSW	4	139,430,176 (GRCm38)	missense	probably damaging	1.00
R0027:Ubr4	UTSW	4	139,400,393 (GRCm38)	missense	probably damaging	0.99
R0027:Ubr4	UTSW	4	139,400,393 (GRCm38)	missense	probably damaging	0.99
R0030:Ubr4	UTSW	4	139,426,793 (GRCm38)	missense	probably damaging	1.00
R0044:Ubr4	UTSW	4	139,437,058 (GRCm38)	splice site	probably benign
R0044:Ubr4	UTSW	4	139,437,058 (GRCm38)	splice site	probably benign
R0088:Ubr4	UTSW	4	139,440,814 (GRCm38)	missense	probably damaging	1.00
R0131:Ubr4	UTSW	4	139,464,051 (GRCm38)	missense	possibly damaging	0.66
R0184:Ubr4	UTSW	4	139,445,262 (GRCm38)	missense	probably damaging	1.00
R0219:Ubr4	UTSW	4	139,430,257 (GRCm38)	missense	possibly damaging	0.64
R0227:Ubr4	UTSW	4	139,431,649 (GRCm38)	missense	probably benign	0.03
R0270:Ubr4	UTSW	4	139,479,435 (GRCm38)	splice site	probably benign
R0285:Ubr4	UTSW	4	139,440,801 (GRCm38)	missense	probably damaging	1.00
R0322:Ubr4	UTSW	4	139,422,418 (GRCm38)	missense	probably damaging	1.00
R0363:Ubr4	UTSW	4	139,391,860 (GRCm38)	missense	probably damaging	0.98
R0393:Ubr4	UTSW	4	139,410,860 (GRCm38)	splice site	probably benign
R0450:Ubr4	UTSW	4	139,430,223 (GRCm38)	missense	probably benign	0.14
R0504:Ubr4	UTSW	4	139,406,578 (GRCm38)	missense	probably damaging	1.00
R0504:Ubr4	UTSW	4	139,480,838 (GRCm38)	critical splice donor site	probably null
R0510:Ubr4	UTSW	4	139,430,223 (GRCm38)	missense	probably benign	0.14
R0513:Ubr4	UTSW	4	139,416,875 (GRCm38)	missense	possibly damaging	0.74
R0517:Ubr4	UTSW	4	139,392,124 (GRCm38)	missense	probably benign	0.00
R0558:Ubr4	UTSW	4	139,426,902 (GRCm38)	missense	probably benign	0.09
R0617:Ubr4	UTSW	4	139,479,062 (GRCm38)	critical splice donor site	probably null
R0636:Ubr4	UTSW	4	139,436,302 (GRCm38)	splice site	probably null
R0637:Ubr4	UTSW	4	139,399,615 (GRCm38)	missense	probably damaging	1.00
R0652:Ubr4	UTSW	4	139,401,326 (GRCm38)	missense	probably damaging	0.99
R0691:Ubr4	UTSW	4	139,423,906 (GRCm38)	missense	probably damaging	1.00
R0729:Ubr4	UTSW	4	139,485,320 (GRCm38)	missense	possibly damaging	0.66
R0735:Ubr4	UTSW	4	139,428,028 (GRCm38)	splice site	probably null
R0751:Ubr4	UTSW	4	139,437,198 (GRCm38)	splice site	probably benign
R0789:Ubr4	UTSW	4	139,410,271 (GRCm38)	critical splice donor site	probably null
R0825:Ubr4	UTSW	4	139,479,576 (GRCm38)	critical splice donor site	probably null
R0828:Ubr4	UTSW	4	139,450,553 (GRCm38)	splice site	probably benign
R1052:Ubr4	UTSW	4	139,455,460 (GRCm38)	missense	possibly damaging	0.83
R1184:Ubr4	UTSW	4	139,437,198 (GRCm38)	splice site	probably benign
R1222:Ubr4	UTSW	4	139,388,471 (GRCm38)	splice site	probably null
R1258:Ubr4	UTSW	4	139,426,914 (GRCm38)	missense	probably damaging	1.00
R1321:Ubr4	UTSW	4	139,460,123 (GRCm38)	missense	possibly damaging	0.46
R1385:Ubr4	UTSW	4	139,402,612 (GRCm38)	missense	probably benign	0.00
R1450:Ubr4	UTSW	4	139,468,028 (GRCm38)	missense	probably damaging	1.00
R1470:Ubr4	UTSW	4	139,421,226 (GRCm38)	splice site	probably null
R1470:Ubr4	UTSW	4	139,421,226 (GRCm38)	splice site	probably null
R1474:Ubr4	UTSW	4	139,429,579 (GRCm38)	missense	probably damaging	1.00
R1479:Ubr4	UTSW	4	139,425,840 (GRCm38)	missense	possibly damaging	0.95
R1534:Ubr4	UTSW	4	139,428,151 (GRCm38)	missense	possibly damaging	0.95
R1546:Ubr4	UTSW	4	139,416,927 (GRCm38)	nonsense	probably null
R1785:Ubr4	UTSW	4	139,423,945 (GRCm38)	missense	probably damaging	1.00
R1786:Ubr4	UTSW	4	139,423,945 (GRCm38)	missense	probably damaging	1.00
R1789:Ubr4	UTSW	4	139,393,053 (GRCm38)	missense	probably damaging	1.00
R1796:Ubr4	UTSW	4	139,428,596 (GRCm38)	missense	probably benign	0.25
R1800:Ubr4	UTSW	4	139,407,963 (GRCm38)	missense	probably damaging	0.99
R1801:Ubr4	UTSW	4	139,452,563 (GRCm38)	splice site	probably null
R1827:Ubr4	UTSW	4	139,425,697 (GRCm38)	critical splice acceptor site	probably null
R1887:Ubr4	UTSW	4	139,455,560 (GRCm38)	missense	probably damaging	1.00
R1966:Ubr4	UTSW	4	139,451,244 (GRCm38)	critical splice acceptor site	probably null
R2010:Ubr4	UTSW	4	139,480,652 (GRCm38)	missense	possibly damaging	0.92
R2049:Ubr4	UTSW	4	139,477,207 (GRCm38)	missense	probably damaging	0.97
R2069:Ubr4	UTSW	4	139,479,540 (GRCm38)	missense	possibly damaging	0.66
R2114:Ubr4	UTSW	4	139,429,611 (GRCm38)	nonsense	probably null
R2140:Ubr4	UTSW	4	139,477,207 (GRCm38)	missense	probably damaging	0.97
R2141:Ubr4	UTSW	4	139,477,207 (GRCm38)	missense	probably damaging	0.97
R2142:Ubr4	UTSW	4	139,477,207 (GRCm38)	missense	probably damaging	0.97
R2168:Ubr4	UTSW	4	139,410,649 (GRCm38)	missense	probably benign	0.25
R2237:Ubr4	UTSW	4	139,442,790 (GRCm38)	missense	probably damaging	1.00
R2249:Ubr4	UTSW	4	139,448,921 (GRCm38)	missense	probably damaging	1.00
R2261:Ubr4	UTSW	4	139,413,462 (GRCm38)	missense	probably damaging	0.99
R2264:Ubr4	UTSW	4	139,420,373 (GRCm38)	splice site	probably benign
R2353:Ubr4	UTSW	4	139,433,673 (GRCm38)	missense	possibly damaging	0.48
R2437:Ubr4	UTSW	4	139,473,542 (GRCm38)	missense	possibly damaging	0.90
R2496:Ubr4	UTSW	4	139,473,205 (GRCm38)	unclassified	probably benign
R2896:Ubr4	UTSW	4	139,455,644 (GRCm38)	splice site	probably null
R2922:Ubr4	UTSW	4	139,479,500 (GRCm38)	missense	possibly damaging	0.66
R2972:Ubr4	UTSW	4	139,406,536 (GRCm38)	missense	probably benign	0.22
R2973:Ubr4	UTSW	4	139,406,536 (GRCm38)	missense	probably benign	0.22
R2989:Ubr4	UTSW	4	139,463,558 (GRCm38)	missense	possibly damaging	0.89
R3176:Ubr4	UTSW	4	139,421,855 (GRCm38)	missense	probably benign	0.03
R3276:Ubr4	UTSW	4	139,421,855 (GRCm38)	missense	probably benign	0.03
R3772:Ubr4	UTSW	4	139,452,700 (GRCm38)	missense	possibly damaging	0.89
R3844:Ubr4	UTSW	4	139,459,126 (GRCm38)	missense	probably damaging	0.99
R3873:Ubr4	UTSW	4	139,423,990 (GRCm38)	missense	probably damaging	1.00
R3900:Ubr4	UTSW	4	139,479,062 (GRCm38)	critical splice donor site	probably null
R3951:Ubr4	UTSW	4	139,393,094 (GRCm38)	missense	probably damaging	1.00
R4020:Ubr4	UTSW	4	139,451,805 (GRCm38)	missense	probably damaging	0.98
R4178:Ubr4	UTSW	4	139,393,414 (GRCm38)	missense	probably damaging	1.00
R4308:Ubr4	UTSW	4	139,472,509 (GRCm38)	missense	possibly damaging	0.46
R4378:Ubr4	UTSW	4	139,410,440 (GRCm38)	missense	possibly damaging	0.76
R4400:Ubr4	UTSW	4	139,461,856 (GRCm38)	missense	possibly damaging	0.66
R4421:Ubr4	UTSW	4	139,461,856 (GRCm38)	missense	possibly damaging	0.66
R4462:Ubr4	UTSW	4	139,418,502 (GRCm38)	missense	possibly damaging	0.47
R4583:Ubr4	UTSW	4	139,380,853 (GRCm38)	missense	possibly damaging	0.82
R4611:Ubr4	UTSW	4	139,399,579 (GRCm38)	missense	possibly damaging	0.93
R4664:Ubr4	UTSW	4	139,406,518 (GRCm38)	missense	possibly damaging	0.56
R4671:Ubr4	UTSW	4	139,436,191 (GRCm38)	missense	possibly damaging	0.66
R4672:Ubr4	UTSW	4	139,410,716 (GRCm38)	missense	probably damaging	0.99
R4673:Ubr4	UTSW	4	139,410,716 (GRCm38)	missense	probably damaging	0.99
R4696:Ubr4	UTSW	4	139,408,672 (GRCm38)	missense	probably benign	0.09
R4701:Ubr4	UTSW	4	139,471,336 (GRCm38)	missense	possibly damaging	0.66
R4705:Ubr4	UTSW	4	139,450,529 (GRCm38)	missense	probably damaging	1.00
R4726:Ubr4	UTSW	4	139,482,579 (GRCm38)	missense	possibly damaging	0.46
R4728:Ubr4	UTSW	4	139,423,879 (GRCm38)	missense	probably damaging	1.00
R4783:Ubr4	UTSW	4	139,421,733 (GRCm38)	missense	possibly damaging	0.85
R4833:Ubr4	UTSW	4	139,402,546 (GRCm38)	missense	probably damaging	0.98
R4892:Ubr4	UTSW	4	139,428,517 (GRCm38)	missense	probably benign	0.14
R4936:Ubr4	UTSW	4	139,396,566 (GRCm38)	missense	probably damaging	0.98
R5000:Ubr4	UTSW	4	139,436,169 (GRCm38)	missense	probably damaging	0.98
R5114:Ubr4	UTSW	4	139,410,623 (GRCm38)	missense	probably damaging	0.99
R5189:Ubr4	UTSW	4	139,410,649 (GRCm38)	missense	probably benign	0.25
R5197:Ubr4	UTSW	4	139,468,097 (GRCm38)	missense	probably damaging	1.00
R5213:Ubr4	UTSW	4	139,402,566 (GRCm38)	nonsense	probably null
R5219:Ubr4	UTSW	4	139,477,232 (GRCm38)	nonsense	probably null
R5346:Ubr4	UTSW	4	139,428,491 (GRCm38)	missense	probably damaging	0.97
R5368:Ubr4	UTSW	4	139,397,528 (GRCm38)	intron	probably benign
R5442:Ubr4	UTSW	4	139,407,772 (GRCm38)	missense	probably damaging	1.00
R5527:Ubr4	UTSW	4	139,480,788 (GRCm38)	missense	possibly damaging	0.83
R5548:Ubr4	UTSW	4	139,460,090 (GRCm38)	missense	probably damaging	0.97
R5568:Ubr4	UTSW	4	139,392,038 (GRCm38)	missense	probably damaging	0.99
R5639:Ubr4	UTSW	4	139,452,648 (GRCm38)	missense	possibly damaging	0.66
R5643:Ubr4	UTSW	4	139,444,687 (GRCm38)	missense	probably damaging	1.00
R5663:Ubr4	UTSW	4	139,428,583 (GRCm38)	missense	possibly damaging	0.95
R5755:Ubr4	UTSW	4	139,460,095 (GRCm38)	missense	possibly damaging	0.66
R5781:Ubr4	UTSW	4	139,468,096 (GRCm38)	missense	probably damaging	1.00
R5784:Ubr4	UTSW	4	139,425,218 (GRCm38)	missense	probably damaging	1.00
R5817:Ubr4	UTSW	4	139,468,847 (GRCm38)	missense	probably damaging	1.00
R5872:Ubr4	UTSW	4	139,425,330 (GRCm38)	missense	probably damaging	0.98
R5891:Ubr4	UTSW	4	139,408,626 (GRCm38)	nonsense	probably null
R5901:Ubr4	UTSW	4	139,451,254 (GRCm38)	missense	probably damaging	1.00
R5958:Ubr4	UTSW	4	139,455,638 (GRCm38)	missense	probably damaging	1.00
R5974:Ubr4	UTSW	4	139,421,078 (GRCm38)	splice site	probably null
R6065:Ubr4	UTSW	4	139,421,238 (GRCm38)	missense	probably damaging	1.00
R6109:Ubr4	UTSW	4	139,417,364 (GRCm38)	missense	probably damaging	0.99
R6207:Ubr4	UTSW	4	139,421,248 (GRCm38)	missense	probably damaging	1.00
R6265:Ubr4	UTSW	4	139,452,640 (GRCm38)	missense	possibly damaging	0.90
R6319:Ubr4	UTSW	4	139,408,889 (GRCm38)	missense	possibly damaging	0.84
R6342:Ubr4	UTSW	4	139,429,539 (GRCm38)	missense	possibly damaging	0.88
R6434:Ubr4	UTSW	4	139,429,638 (GRCm38)	missense	probably damaging	1.00
R6437:Ubr4	UTSW	4	139,397,214 (GRCm38)	critical splice donor site	probably null
R6481:Ubr4	UTSW	4	139,431,751 (GRCm38)	missense	probably damaging	0.97
R6502:Ubr4	UTSW	4	139,444,671 (GRCm38)	missense	probably damaging	1.00
R6546:Ubr4	UTSW	4	139,414,394 (GRCm38)	missense	probably damaging	0.99
R6603:Ubr4	UTSW	4	139,455,586 (GRCm38)	missense	probably benign	0.17
R6648:Ubr4	UTSW	4	139,452,719 (GRCm38)	nonsense	probably null
R6649:Ubr4	UTSW	4	139,473,624 (GRCm38)	missense	possibly damaging	0.66
R6653:Ubr4	UTSW	4	139,473,624 (GRCm38)	missense	possibly damaging	0.66
R6668:Ubr4	UTSW	4	139,465,341 (GRCm38)	missense	probably damaging	1.00
R6770:Ubr4	UTSW	4	139,489,182 (GRCm38)	missense	unknown
R6772:Ubr4	UTSW	4	139,467,230 (GRCm38)	nonsense	probably null
R6857:Ubr4	UTSW	4	139,486,051 (GRCm38)	missense	possibly damaging	0.90
R6869:Ubr4	UTSW	4	139,467,227 (GRCm38)	missense	possibly damaging	0.93
R6912:Ubr4	UTSW	4	139,458,234 (GRCm38)	critical splice donor site	probably null
R6943:Ubr4	UTSW	4	139,437,131 (GRCm38)	nonsense	probably null
R6970:Ubr4	UTSW	4	139,406,528 (GRCm38)	nonsense	probably null
R6976:Ubr4	UTSW	4	139,393,077 (GRCm38)	missense	probably damaging	0.98
R7000:Ubr4	UTSW	4	139,414,404 (GRCm38)	missense	probably damaging	1.00
R7017:Ubr4	UTSW	4	139,393,090 (GRCm38)	missense	probably damaging	0.99
R7165:Ubr4	UTSW	4	139,450,513 (GRCm38)	missense
R7222:Ubr4	UTSW	4	139,463,373 (GRCm38)	missense	unknown
R7241:Ubr4	UTSW	4	139,443,414 (GRCm38)	missense	probably damaging	1.00
R7343:Ubr4	UTSW	4	139,413,438 (GRCm38)	missense	probably benign	0.09
R7367:Ubr4	UTSW	4	139,452,691 (GRCm38)	missense	unknown
R7393:Ubr4	UTSW	4	139,426,785 (GRCm38)	missense	probably damaging	1.00
R7432:Ubr4	UTSW	4	139,388,382 (GRCm38)	missense	probably damaging	1.00
R7448:Ubr4	UTSW	4	139,462,467 (GRCm38)	missense	unknown
R7502:Ubr4	UTSW	4	139,412,672 (GRCm38)	missense	possibly damaging	0.72
R7514:Ubr4	UTSW	4	139,452,655 (GRCm38)	missense	unknown
R7526:Ubr4	UTSW	4	139,422,417 (GRCm38)	missense	probably benign	0.00
R7529:Ubr4	UTSW	4	139,422,417 (GRCm38)	missense	probably benign	0.00
R7666:Ubr4	UTSW	4	139,413,480 (GRCm38)	missense	possibly damaging	0.81
R7699:Ubr4	UTSW	4	139,408,867 (GRCm38)	nonsense	probably null
R7700:Ubr4	UTSW	4	139,408,867 (GRCm38)	nonsense	probably null
R7726:Ubr4	UTSW	4	139,458,920 (GRCm38)	missense	unknown
R7753:Ubr4	UTSW	4	139,470,292 (GRCm38)	missense	unknown
R7810:Ubr4	UTSW	4	139,415,083 (GRCm38)	missense
R7837:Ubr4	UTSW	4	139,393,151 (GRCm38)	nonsense	probably null
R7868:Ubr4	UTSW	4	139,460,033 (GRCm38)	missense	unknown
R7872:Ubr4	UTSW	4	139,393,062 (GRCm38)	missense	possibly damaging	0.94
R7887:Ubr4	UTSW	4	139,407,810 (GRCm38)	missense	probably damaging	0.99
R7924:Ubr4	UTSW	4	139,467,276 (GRCm38)	missense	unknown
R7982:Ubr4	UTSW	4	139,428,208 (GRCm38)	critical splice donor site	probably null
R8005:Ubr4	UTSW	4	139,412,630 (GRCm38)	missense	probably damaging	0.98
R8054:Ubr4	UTSW	4	139,468,102 (GRCm38)	missense	unknown
R8094:Ubr4	UTSW	4	139,440,696 (GRCm38)	missense	probably damaging	0.97
R8151:Ubr4	UTSW	4	139,402,801 (GRCm38)	missense	probably damaging	0.97
R8183:Ubr4	UTSW	4	139,482,471 (GRCm38)	missense	unknown
R8252:Ubr4	UTSW	4	139,473,217 (GRCm38)	missense	unknown
R8505:Ubr4	UTSW	4	139,429,569 (GRCm38)	missense
R8519:Ubr4	UTSW	4	139,416,647 (GRCm38)	missense	probably damaging	0.97
R8716:Ubr4	UTSW	4	139,468,853 (GRCm38)	missense	unknown
R8720:Ubr4	UTSW	4	139,480,838 (GRCm38)	critical splice donor site	probably null
R8749:Ubr4	UTSW	4	139,402,624 (GRCm38)	missense	probably damaging	0.98
R8768:Ubr4	UTSW	4	139,421,765 (GRCm38)	missense
R8789:Ubr4	UTSW	4	139,410,183 (GRCm38)	missense	possibly damaging	0.76
R8879:Ubr4	UTSW	4	139,410,518 (GRCm38)	missense	probably benign	0.06
R8937:Ubr4	UTSW	4	139,463,575 (GRCm38)	missense	probably damaging	0.97
R9006:Ubr4	UTSW	4	139,444,692 (GRCm38)	critical splice donor site	probably null
R9116:Ubr4	UTSW	4	139,418,474 (GRCm38)	missense
R9134:Ubr4	UTSW	4	139,400,444 (GRCm38)	critical splice donor site	probably null
R9251:Ubr4	UTSW	4	139,450,325 (GRCm38)	missense
R9340:Ubr4	UTSW	4	139,455,452 (GRCm38)	missense	unknown
R9384:Ubr4	UTSW	4	139,467,320 (GRCm38)	missense	unknown
R9389:Ubr4	UTSW	4	139,425,924 (GRCm38)	missense
R9393:Ubr4	UTSW	4	139,485,302 (GRCm38)	missense	unknown
R9531:Ubr4	UTSW	4	139,407,906 (GRCm38)	missense	probably benign	0.00
R9573:Ubr4	UTSW	4	139,421,139 (GRCm38)	missense
R9604:Ubr4	UTSW	4	139,432,516 (GRCm38)	critical splice donor site	probably null
R9613:Ubr4	UTSW	4	139,421,762 (GRCm38)	missense
R9623:Ubr4	UTSW	4	139,431,713 (GRCm38)	missense	probably benign	0.00
R9651:Ubr4	UTSW	4	139,479,548 (GRCm38)	missense	unknown
R9685:Ubr4	UTSW	4	139,464,030 (GRCm38)	missense	unknown
R9698:Ubr4	UTSW	4	139,440,664 (GRCm38)	missense
R9700:Ubr4	UTSW	4	139,392,077 (GRCm38)	missense	probably damaging	0.97
R9727:Ubr4	UTSW	4	139,413,424 (GRCm38)	missense	probably damaging	0.98
R9766:Ubr4	UTSW	4	139,467,284 (GRCm38)	missense	unknown
T0975:Ubr4	UTSW	4	139,451,781 (GRCm38)	missense	probably damaging	1.00
X0028:Ubr4	UTSW	4	139,410,271 (GRCm38)	critical splice donor site	probably null
Z1176:Ubr4	UTSW	4	139,410,145 (GRCm38)	missense	probably damaging	1.00
Z1176:Ubr4	UTSW	4	139,402,660 (GRCm38)	missense	probably benign	0.04
Z1176:Ubr4	UTSW	4	139,400,385 (GRCm38)	missense	probably damaging	1.00
Z1177:Ubr4	UTSW	4	139,450,481 (GRCm38)	missense
Z1186:Ubr4	UTSW	4	139,410,653 (GRCm38)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- ACACTGCCAGTTTCATGCATG -3'
(R):5'- ACTGCAGCATTGTGAAGCTG -3'

Sequencing Primer
(F):5'- CTCACTCTGTAGAAAAGGTTGGCC -3'
(R):5'- CATTGTGAAGCTGAAGGTGGCC -3'

Posted On

2020-09-15