Mutagenetix > Incidental Mutation

Incidental Mutation 'R7980:Pum2'

651168

Institutional Source

Beutler Lab

Gene Symbol

Pum2

Ensembl Gene

ENSMUSG00000020594

Gene Name

pumilio RNA-binding family member 2

Synonyms

Pumm2, 5730503J23Rik

MMRRC Submission

046021-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7980 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

8724134-8802581 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 8763904 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 283 (Y283H)

Ref Sequence

ENSEMBL: ENSMUSP00000131074 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020915] [ENSMUST00000111122] [ENSMUST00000111123] [ENSMUST00000163569] [ENSMUST00000163730] [ENSMUST00000165293] [ENSMUST00000166965] [ENSMUST00000168361] [ENSMUST00000169089] [ENSMUST00000169750] [ENSMUST00000170037] [ENSMUST00000178015]

AlphaFold

Q80U58

PDB Structure

Structure and RNA binding of the mouse Pumilio-2 Puf Domain [X-RAY DIFFRACTION]
Structure and RNA binding of the mouse Pumilio-2 Puf Domain [X-RAY DIFFRACTION]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000020915
AA Change: Y283H

PolyPhen 2 Score 0.838 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000020915
Gene: ENSMUSG00000020594
AA Change: Y283H

Domain	Start	End	E-Value	Type
low complexity region	193	206	N/A	INTRINSIC
low complexity region	269	290	N/A	INTRINSIC
low complexity region	319	336	N/A	INTRINSIC
low complexity region	353	378	N/A	INTRINSIC
low complexity region	464	490	N/A	INTRINSIC
low complexity region	498	509	N/A	INTRINSIC
low complexity region	521	535	N/A	INTRINSIC
low complexity region	542	576	N/A	INTRINSIC
low complexity region	591	609	N/A	INTRINSIC
Pumilio	642	677	2.35e-7	SMART
Pumilio	678	713	6.54e-6	SMART
Pumilio	714	749	2.89e-7	SMART
Pumilio	750	785	3.37e-8	SMART
Pumilio	786	821	4.84e-9	SMART
Pumilio	822	857	3.2e-9	SMART
Pumilio	858	893	5.78e-7	SMART
Pumilio	901	936	3.62e-8	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000111122
AA Change: Y283H

PolyPhen 2 Score 0.933 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000106751
Gene: ENSMUSG00000020594
AA Change: Y283H

Domain	Start	End	E-Value	Type
low complexity region	193	206	N/A	INTRINSIC
low complexity region	269	290	N/A	INTRINSIC
low complexity region	324	341	N/A	INTRINSIC
low complexity region	358	383	N/A	INTRINSIC
low complexity region	469	495	N/A	INTRINSIC
low complexity region	503	514	N/A	INTRINSIC
low complexity region	526	540	N/A	INTRINSIC
low complexity region	547	602	N/A	INTRINSIC
low complexity region	630	660	N/A	INTRINSIC
low complexity region	675	693	N/A	INTRINSIC
Pumilio	726	761	2.35e-7	SMART
Pumilio	762	797	6.54e-6	SMART
Pumilio	798	833	2.89e-7	SMART
Pumilio	834	869	3.37e-8	SMART
Pumilio	870	905	4.84e-9	SMART
Pumilio	906	941	3.2e-9	SMART
Pumilio	942	977	5.78e-7	SMART
Pumilio	985	1020	3.62e-8	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000111123
AA Change: Y283H

PolyPhen 2 Score 0.933 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000106752
Gene: ENSMUSG00000020594
AA Change: Y283H

Domain	Start	End	E-Value	Type
low complexity region	193	206	N/A	INTRINSIC
low complexity region	269	290	N/A	INTRINSIC
low complexity region	324	341	N/A	INTRINSIC
low complexity region	358	383	N/A	INTRINSIC
low complexity region	469	495	N/A	INTRINSIC
low complexity region	503	514	N/A	INTRINSIC
low complexity region	526	540	N/A	INTRINSIC
low complexity region	547	602	N/A	INTRINSIC
low complexity region	630	660	N/A	INTRINSIC
low complexity region	675	693	N/A	INTRINSIC
Pumilio	726	761	2.35e-7	SMART
Pumilio	762	797	6.54e-6	SMART
Pumilio	798	833	2.89e-7	SMART
Pumilio	834	869	3.37e-8	SMART
Pumilio	870	905	4.84e-9	SMART
Pumilio	906	941	3.2e-9	SMART
Pumilio	942	977	5.78e-7	SMART
Pumilio	985	1020	3.62e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000163569
AA Change: Y283H

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000131074
Gene: ENSMUSG00000020594
AA Change: Y283H

Domain	Start	End	E-Value	Type
low complexity region	193	206	N/A	INTRINSIC
low complexity region	269	290	N/A	INTRINSIC
low complexity region	324	341	N/A	INTRINSIC
low complexity region	358	383	N/A	INTRINSIC
low complexity region	469	495	N/A	INTRINSIC
low complexity region	503	514	N/A	INTRINSIC
low complexity region	526	540	N/A	INTRINSIC
low complexity region	547	602	N/A	INTRINSIC
low complexity region	630	660	N/A	INTRINSIC
low complexity region	675	693	N/A	INTRINSIC
Pumilio	726	761	2.35e-7	SMART
Pumilio	762	797	6.54e-6	SMART
Pumilio	798	832	1.29e-4	SMART
Pumilio	836	871	3.37e-8	SMART
Pumilio	872	907	4.84e-9	SMART
Pumilio	908	943	3.2e-9	SMART
Pumilio	944	979	5.78e-7	SMART
Pumilio	987	1022	3.62e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000163730

Predicted Effect

probably benign
Transcript: ENSMUST00000165293

Predicted Effect

probably benign
Transcript: ENSMUST00000166965

Predicted Effect

probably damaging
Transcript: ENSMUST00000168361
AA Change: Y283H

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000128292
Gene: ENSMUSG00000020594
AA Change: Y283H

Domain	Start	End	E-Value	Type
low complexity region	193	206	N/A	INTRINSIC
low complexity region	269	290	N/A	INTRINSIC
low complexity region	324	341	N/A	INTRINSIC
low complexity region	358	383	N/A	INTRINSIC
low complexity region	469	495	N/A	INTRINSIC
low complexity region	503	514	N/A	INTRINSIC
low complexity region	526	540	N/A	INTRINSIC
low complexity region	547	602	N/A	INTRINSIC
low complexity region	630	660	N/A	INTRINSIC
low complexity region	675	693	N/A	INTRINSIC
Pumilio	726	761	2.35e-7	SMART
Pumilio	762	797	6.54e-6	SMART
Pumilio	798	832	1.29e-4	SMART
Pumilio	836	871	3.37e-8	SMART
Pumilio	872	907	4.84e-9	SMART
Pumilio	908	943	3.2e-9	SMART
Pumilio	944	979	5.78e-7	SMART
Pumilio	987	1022	3.62e-8	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000169089
AA Change: Y283H

PolyPhen 2 Score 0.900 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000132122
Gene: ENSMUSG00000020594
AA Change: Y283H

Domain	Start	End	E-Value	Type
low complexity region	193	206	N/A	INTRINSIC
low complexity region	269	290	N/A	INTRINSIC
low complexity region	324	341	N/A	INTRINSIC
low complexity region	358	383	N/A	INTRINSIC
low complexity region	469	495	N/A	INTRINSIC
low complexity region	503	514	N/A	INTRINSIC
low complexity region	526	540	N/A	INTRINSIC
low complexity region	547	581	N/A	INTRINSIC
low complexity region	596	614	N/A	INTRINSIC
Pumilio	647	682	2.35e-7	SMART
Pumilio	683	718	6.54e-6	SMART
Pumilio	719	754	2.89e-7	SMART
Pumilio	755	790	3.37e-8	SMART
Pumilio	791	826	4.84e-9	SMART
Pumilio	827	862	3.2e-9	SMART
Pumilio	863	898	5.78e-7	SMART
Pumilio	906	941	3.62e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000169750

SMART Domains

Protein: ENSMUSP00000126876
Gene: ENSMUSG00000020594

Domain	Start	End	E-Value	Type
low complexity region	193	206	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000170037

Predicted Effect

SMART Domains

Protein: ENSMUSP00000126616
Gene: ENSMUSG00000020594
AA Change: Y144H

Domain	Start	End	E-Value	Type
low complexity region	55	68	N/A	INTRINSIC
low complexity region	131	152	N/A	INTRINSIC
low complexity region	180	197	N/A	INTRINSIC
low complexity region	214	234	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000178015
AA Change: Y283H

PolyPhen 2 Score 0.900 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000137020
Gene: ENSMUSG00000020594
AA Change: Y283H

Domain	Start	End	E-Value	Type
low complexity region	193	206	N/A	INTRINSIC
low complexity region	269	290	N/A	INTRINSIC
low complexity region	324	341	N/A	INTRINSIC
low complexity region	358	383	N/A	INTRINSIC
low complexity region	469	495	N/A	INTRINSIC
low complexity region	503	514	N/A	INTRINSIC
low complexity region	526	540	N/A	INTRINSIC
low complexity region	547	581	N/A	INTRINSIC
low complexity region	596	614	N/A	INTRINSIC
Pumilio	647	682	2.35e-7	SMART
Pumilio	683	718	6.54e-6	SMART
Pumilio	719	754	2.89e-7	SMART
Pumilio	755	790	3.37e-8	SMART
Pumilio	791	826	4.84e-9	SMART
Pumilio	827	862	3.2e-9	SMART
Pumilio	863	898	5.78e-7	SMART
Pumilio	906	941	3.62e-8	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (87/87)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
PHENOTYPE: Mice homozygous for a gene trapped allele exhibit significantly smaller testes and seminiferous tubule degeneration but are otherwise viable and fertile. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930553M12Rik	A	G	4: 88,786,315 (GRCm39)	L101P	unknown	Het
Actn3	G	A	19: 4,917,950 (GRCm39)	P339L	probably damaging	Het
Adamts12	G	A	15: 11,263,423 (GRCm39)	C595Y	probably damaging	Het
Adrm1b	T	A	3: 92,336,406 (GRCm39)	K99*	probably null	Het
Agl	A	T	3: 116,585,830 (GRCm39)	N99K	probably benign	Het
Aplp1	A	T	7: 30,134,992 (GRCm39)	M592K	probably benign	Het
Arhgef11	C	T	3: 87,605,297 (GRCm39)	R251C	probably benign	Het
Arhgef12	A	T	9: 42,882,595 (GRCm39)	C1416*	probably null	Het
Asah1	A	G	8: 41,807,067 (GRCm39)	M119T		Het
Asxl2	A	T	12: 3,546,630 (GRCm39)	Q471H	probably damaging	Het
Atp6v1c2	A	T	12: 17,371,613 (GRCm39)	D61E	probably damaging	Het
Btbd16	C	A	7: 130,426,097 (GRCm39)	P520Q	probably damaging	Het
Cacnb2	A	G	2: 14,609,326 (GRCm39)	E22G	probably benign	Het
Catspere2	G	T	1: 177,830,610 (GRCm39)		probably null	Het
Cdc25a	T	A	9: 109,708,949 (GRCm39)	D124E	probably damaging	Het
Cdc42se1	A	T	3: 95,139,166 (GRCm39)		probably benign	Het
Cdcp3	T	A	7: 130,836,506 (GRCm39)	V400E	probably damaging	Het
Cfap54	T	C	10: 92,817,922 (GRCm39)	K1265E	possibly damaging	Het
Chaf1b	A	G	16: 93,681,415 (GRCm39)	H11R	probably damaging	Het
Chil4	T	C	3: 106,110,060 (GRCm39)	K345E	probably damaging	Het
Cntnap1	A	G	11: 101,079,719 (GRCm39)	I1283V	probably benign	Het
Dagla	C	T	19: 10,229,406 (GRCm39)	C618Y	possibly damaging	Het
Ddx19b	C	T	8: 111,738,077 (GRCm39)	V224M	possibly damaging	Het
Dohh	C	T	10: 81,223,726 (GRCm39)	R260*	probably null	Het
Dsg3	A	T	18: 20,664,417 (GRCm39)	N472Y	probably benign	Het
Duox1	T	A	2: 122,177,801 (GRCm39)	N1528K	possibly damaging	Het
Eml6	A	G	11: 29,783,205 (GRCm39)	Y559H	probably damaging	Het
Esp4	A	G	17: 40,913,192 (GRCm39)	T20A	possibly damaging	Het
Fbxw21	T	C	9: 108,985,639 (GRCm39)		probably null	Het
Gcc2	T	A	10: 58,114,574 (GRCm39)		probably null	Het
Grin3b	C	T	10: 79,811,559 (GRCm39)	A715V	possibly damaging	Het
Gxylt2	A	T	6: 100,764,170 (GRCm39)		probably null	Het
Hspa4	A	T	11: 53,171,404 (GRCm39)	S267T	probably benign	Het
Hspd1	A	G	1: 55,117,785 (GRCm39)	V491A	possibly damaging	Het
Idua	A	G	5: 108,828,486 (GRCm39)	E280G	probably benign	Het
Ift172	T	A	5: 31,417,988 (GRCm39)	E1267V	probably benign	Het
Itsn1	T	A	16: 91,702,182 (GRCm39)	V1448E	unknown	Het
Kat6a	A	G	8: 23,416,432 (GRCm39)	M647V	possibly damaging	Het
Kcnq4	A	T	4: 120,568,494 (GRCm39)	D407E	probably benign	Het
Kif1b	A	T	4: 149,354,378 (GRCm39)	F221I	probably damaging	Het
Lama2	G	T	10: 27,239,609 (GRCm39)	D315E	probably damaging	Het
Lrrn4	G	A	2: 132,720,096 (GRCm39)	L235F	probably damaging	Het
Map2k6	T	A	11: 110,390,210 (GRCm39)	I248N		Het
Mgat5	A	G	1: 127,407,248 (GRCm39)	Q638R	probably benign	Het
Mia2	A	G	12: 59,155,651 (GRCm39)	K455E	probably damaging	Het
Mrs2	A	G	13: 25,204,221 (GRCm39)	C3R	possibly damaging	Het
Nprl3	A	G	11: 32,187,357 (GRCm39)	I325T	probably damaging	Het
Nr1h3	T	A	2: 91,021,229 (GRCm39)	Q186L	probably benign	Het
Oas1f	A	G	5: 120,989,538 (GRCm39)	E159G	probably benign	Het
Or2g7	A	T	17: 38,378,412 (GRCm39)	M117L	possibly damaging	Het
Or4k15b	T	A	14: 50,272,252 (GRCm39)	S203C	probably damaging	Het
Or52ae7	T	A	7: 103,119,504 (GRCm39)	F86Y	probably damaging	Het
Or52e19b	T	A	7: 103,032,970 (GRCm39)	I80F	probably damaging	Het
Or52s1b	T	A	7: 102,822,242 (GRCm39)	S201C	probably damaging	Het
Or5m12	A	G	2: 85,734,942 (GRCm39)	F152S	probably benign	Het
Or8g4	T	C	9: 39,662,417 (GRCm39)	I245T	probably damaging	Het
Or9a4	T	A	6: 40,549,154 (GRCm39)	I278N	probably benign	Het
Pbxip1	G	C	3: 89,353,648 (GRCm39)	S267T	probably benign	Het
Pcdhga10	A	T	18: 37,881,645 (GRCm39)	I469F	possibly damaging	Het
Pkd1l1	A	G	11: 8,804,375 (GRCm39)	S1739P	probably damaging	Het
Plcd4	A	C	1: 74,604,464 (GRCm39)	N788T	probably benign	Het
Pms2	T	A	5: 143,867,909 (GRCm39)	W838R	probably damaging	Het
Pnpla6	T	A	8: 3,586,562 (GRCm39)	V926D	probably damaging	Het
Pros1	A	T	16: 62,748,516 (GRCm39)	H663L	possibly damaging	Het
Prss55	T	C	14: 64,316,138 (GRCm39)		probably null	Het
Rad21	A	G	15: 51,828,422 (GRCm39)	S549P	probably benign	Het
Sap130	T	A	18: 31,781,182 (GRCm39)		probably null	Het
Sertad4	C	T	1: 192,529,189 (GRCm39)	S209N	probably benign	Het
Sez6	A	G	11: 77,844,668 (GRCm39)	T164A	probably benign	Het
Slc22a28	C	T	19: 8,078,837 (GRCm39)	R284Q	probably damaging	Het
Slco4c1	T	C	1: 96,764,650 (GRCm39)	K474R	probably benign	Het
Stx5a	T	C	19: 8,719,802 (GRCm39)	S56P	probably damaging	Het
Svopl	T	C	6: 37,991,744 (GRCm39)	T379A	probably damaging	Het
Sypl2	A	G	3: 108,125,008 (GRCm39)	F118L	probably damaging	Het
Tfrc	G	A	16: 32,435,967 (GRCm39)	V215M	probably benign	Het
Thnsl2	T	C	6: 71,115,652 (GRCm39)	N185S	probably damaging	Het
Tmem104	T	C	11: 115,134,580 (GRCm39)	I372T	probably damaging	Het
Tnfaip6	G	A	2: 51,941,070 (GRCm39)	G204S	probably damaging	Het
Trim12a	C	T	7: 103,953,335 (GRCm39)	E259K	probably benign	Het
Tubgcp6	G	A	15: 88,986,232 (GRCm39)	R1436C	probably benign	Het
Tyrp1	C	T	4: 80,758,864 (GRCm39)	L246F	probably damaging	Het
Ubr4	G	T	4: 139,145,717 (GRCm39)	V216F		Het
Unc5a	A	G	13: 55,147,319 (GRCm39)	I409V	possibly damaging	Het
Vmn1r15	T	C	6: 57,235,399 (GRCm39)	L89P	probably damaging	Het
Vmn1r32	A	T	6: 66,530,305 (GRCm39)	L157*	probably null	Het
Zcchc24	A	G	14: 25,720,185 (GRCm39)	Y160H	probably damaging	Het
Zfp184	A	T	13: 22,144,376 (GRCm39)	H694L	probably damaging	Het
Zfp345	A	C	2: 150,314,723 (GRCm39)	Y271*	probably null	Het
Zranb3	T	C	1: 128,030,671 (GRCm39)		probably benign	Het

Other mutations in Pum2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00737:Pum2	APN	12	8,783,381 (GRCm39)	missense	probably damaging	1.00
IGL02118:Pum2	APN	12	8,779,117 (GRCm39)	missense	probably benign	0.31
IGL02185:Pum2	APN	12	8,798,955 (GRCm39)	critical splice donor site	probably null
IGL02528:Pum2	APN	12	8,778,696 (GRCm39)	nonsense	probably null
IGL02718:Pum2	APN	12	8,783,344 (GRCm39)	missense	probably benign	0.02
Plumbat	UTSW	12	8,778,779 (GRCm39)	critical splice donor site	probably null
Pummie	UTSW	12	8,763,906 (GRCm39)	nonsense	probably null
Yorkshire	UTSW	12	8,778,726 (GRCm39)	nonsense	probably null
PIT4366001:Pum2	UTSW	12	8,783,390 (GRCm39)	missense	probably damaging	1.00
R0152:Pum2	UTSW	12	8,778,754 (GRCm39)	missense	possibly damaging	0.94
R0317:Pum2	UTSW	12	8,778,754 (GRCm39)	missense	possibly damaging	0.94
R0357:Pum2	UTSW	12	8,771,785 (GRCm39)	missense	possibly damaging	0.92
R0413:Pum2	UTSW	12	8,763,464 (GRCm39)	missense	probably benign	0.00
R0494:Pum2	UTSW	12	8,771,736 (GRCm39)	nonsense	probably null
R0520:Pum2	UTSW	12	8,771,710 (GRCm39)	missense	probably damaging	1.00
R0727:Pum2	UTSW	12	8,794,465 (GRCm39)	missense	probably damaging	1.00
R1576:Pum2	UTSW	12	8,763,524 (GRCm39)	missense	probably benign	0.01
R2035:Pum2	UTSW	12	8,778,638 (GRCm39)	nonsense	probably null
R2060:Pum2	UTSW	12	8,778,726 (GRCm39)	nonsense	probably null
R2422:Pum2	UTSW	12	8,798,931 (GRCm39)	missense	possibly damaging	0.70
R2437:Pum2	UTSW	12	8,794,654 (GRCm39)	missense	probably benign	0.19
R3767:Pum2	UTSW	12	8,769,076 (GRCm39)	nonsense	probably null
R4715:Pum2	UTSW	12	8,797,272 (GRCm39)	missense	probably damaging	1.00
R5155:Pum2	UTSW	12	8,763,572 (GRCm39)	missense	possibly damaging	0.79
R5226:Pum2	UTSW	12	8,763,458 (GRCm39)	missense	possibly damaging	0.73
R5323:Pum2	UTSW	12	8,794,706 (GRCm39)	missense	probably damaging	1.00
R6250:Pum2	UTSW	12	8,794,755 (GRCm39)	splice site	probably null
R6253:Pum2	UTSW	12	8,798,205 (GRCm39)	missense	probably damaging	1.00
R6508:Pum2	UTSW	12	8,798,861 (GRCm39)	missense	probably benign	0.17
R6953:Pum2	UTSW	12	8,778,779 (GRCm39)	critical splice donor site	probably null
R7135:Pum2	UTSW	12	8,778,952 (GRCm39)	missense	possibly damaging	0.80
R7355:Pum2	UTSW	12	8,763,906 (GRCm39)	nonsense	probably null
R7586:Pum2	UTSW	12	8,797,206 (GRCm39)	missense	probably damaging	1.00
R7683:Pum2	UTSW	12	8,778,922 (GRCm39)	missense	possibly damaging	0.93
R7869:Pum2	UTSW	12	8,763,595 (GRCm39)	missense	probably benign	0.00
R7873:Pum2	UTSW	12	8,798,802 (GRCm39)	missense	possibly damaging	0.94
R8166:Pum2	UTSW	12	8,771,739 (GRCm39)	missense	possibly damaging	0.71
R8316:Pum2	UTSW	12	8,763,456 (GRCm39)	missense	possibly damaging	0.89
R8345:Pum2	UTSW	12	8,759,454 (GRCm39)	missense	probably damaging	0.99
R8418:Pum2	UTSW	12	8,760,245 (GRCm39)	missense	possibly damaging	0.87
R8802:Pum2	UTSW	12	8,778,726 (GRCm39)	nonsense	probably null
R9039:Pum2	UTSW	12	8,794,430 (GRCm39)	missense	probably damaging	1.00
R9207:Pum2	UTSW	12	8,763,904 (GRCm39)	missense	probably damaging	0.98
R9366:Pum2	UTSW	12	8,783,344 (GRCm39)	missense	probably benign	0.02
R9700:Pum2	UTSW	12	8,779,044 (GRCm39)	missense	probably damaging	0.97
X0039:Pum2	UTSW	12	8,778,944 (GRCm39)	small deletion	probably benign

Predicted Primers

PCR Primer
(F):5'- TATGACTCACTAGGAATGATAAGGG -3'
(R):5'- GTAATCTGTTACTACTAACCTCAGAGG -3'

Sequencing Primer
(F):5'- CTAGGAATGATAAGGGTTTTGACAC -3'
(R):5'- CCTCAGAGGTGTTTCAGAAAGTAAC -3'

Posted On

2020-09-15