Incidental Mutation 'R7981:Ccdc150'
| ID |
651191 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Ccdc150
|
| Ensembl Gene |
ENSMUSG00000025983 |
| Gene Name |
coiled-coil domain containing 150 |
| Synonyms |
4930511H11Rik |
| MMRRC Submission |
046022-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.082)
|
| Stock # |
R7981 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
1 |
| Chromosomal Location |
54250683-54368727 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 54368392 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Lysine to Methionine
at position 1109
(K1109M)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000027128
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000027128]
[ENSMUST00000160472]
|
| AlphaFold |
Q8CDI7 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000027128
AA Change: K1109M
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000027128
Gene: ENSMUSG00000025983
AA Change: K1109M
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
160 |
250 |
N/A |
INTRINSIC |
|
coiled coil region
|
288 |
314 |
N/A |
INTRINSIC |
|
coiled coil region
|
418 |
676 |
N/A |
INTRINSIC |
|
coiled coil region
|
727 |
952 |
N/A |
INTRINSIC |
|
coiled coil region
|
985 |
1048 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000160472
|
| SMART Domains |
Protein: ENSMUSP00000125195
Gene: ENSMUSG00000025983
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
160 |
250 |
N/A |
INTRINSIC |
|
coiled coil region
|
288 |
314 |
N/A |
INTRINSIC |
|
coiled coil region
|
418 |
551 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.6%
- 20x: 98.8%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 50 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abca8a |
T |
C |
11: 110,089,913 (GRCm38) |
T100A |
probably benign |
Het |
| Agbl1 |
A |
G |
7: 76,444,840 (GRCm38) |
T740A |
unknown |
Het |
| Aldh1a2 |
A |
T |
9: 71,263,820 (GRCm38) |
I197F |
probably damaging |
Het |
| Ankrd28 |
T |
C |
14: 31,702,157 (GRCm38) |
T1009A |
probably benign |
Het |
| Antxrl |
T |
C |
14: 34,065,881 (GRCm38) |
V287A |
probably damaging |
Het |
| Baiap2l1 |
G |
A |
5: 144,357,890 (GRCm38) |
|
probably benign |
Het |
| Catsperd |
T |
C |
17: 56,631,562 (GRCm38) |
V30A |
possibly damaging |
Het |
| Ccdc28a |
A |
G |
10: 18,218,379 (GRCm38) |
L164P |
probably benign |
Het |
| Cnot1 |
C |
T |
8: 95,763,169 (GRCm38) |
V469M |
probably damaging |
Het |
| Col16a1 |
T |
G |
4: 130,086,554 (GRCm38) |
|
probably null |
Het |
| Coq9 |
A |
G |
8: 94,842,657 (GRCm38) |
H39R |
probably benign |
Het |
| Crh |
T |
A |
3: 19,694,052 (GRCm38) |
E142V |
probably benign |
Het |
| Depdc1a |
A |
G |
3: 159,520,851 (GRCm38) |
N265S |
probably benign |
Het |
| Dlg5 |
T |
C |
14: 24,158,145 (GRCm38) |
T998A |
probably benign |
Het |
| Epg5 |
T |
C |
18: 78,009,714 (GRCm38) |
|
probably null |
Het |
| Fam208a |
T |
C |
14: 27,446,416 (GRCm38) |
V305A |
possibly damaging |
Het |
| Gcc1 |
G |
A |
6: 28,419,141 (GRCm38) |
L398F |
probably benign |
Het |
| Gde1 |
T |
C |
7: 118,689,041 (GRCm38) |
T320A |
probably damaging |
Het |
| Gemin5 |
T |
C |
11: 58,145,405 (GRCm38) |
D704G |
probably damaging |
Het |
| Gfi1 |
G |
A |
5: 107,725,677 (GRCm38) |
|
probably benign |
Het |
| Gm13078 |
T |
G |
4: 143,726,882 (GRCm38) |
F187V |
probably benign |
Het |
| Gm5346 |
A |
G |
8: 43,625,813 (GRCm38) |
F458S |
probably damaging |
Het |
| Gm8298 |
T |
A |
3: 59,876,939 (GRCm38) |
F278I |
probably damaging |
Het |
| Insc |
A |
G |
7: 114,829,067 (GRCm38) |
T92A |
probably damaging |
Het |
| Krtap6-2 |
T |
C |
16: 89,419,674 (GRCm38) |
Y135C |
unknown |
Het |
| Lpcat2 |
T |
C |
8: 92,855,554 (GRCm38) |
S34P |
probably damaging |
Het |
| Mansc1 |
T |
C |
6: 134,610,311 (GRCm38) |
D301G |
possibly damaging |
Het |
| Mbl2 |
C |
A |
19: 30,239,337 (GRCm38) |
T183K |
probably damaging |
Het |
| Mri1 |
A |
C |
8: 84,257,163 (GRCm38) |
V33G |
possibly damaging |
Het |
| Mrps7 |
T |
C |
11: 115,606,861 (GRCm38) |
M184T |
possibly damaging |
Het |
| Mug1 |
A |
T |
6: 121,881,764 (GRCm38) |
Y1147F |
probably damaging |
Het |
| N4bp2 |
C |
T |
5: 65,812,142 (GRCm38) |
H1416Y |
probably benign |
Het |
| Naa15 |
T |
C |
3: 51,458,671 (GRCm38) |
F487S |
probably damaging |
Het |
| Nin |
C |
T |
12: 70,042,817 (GRCm38) |
V1275I |
|
Het |
| Olfr1221 |
A |
G |
2: 89,112,056 (GRCm38) |
F152S |
probably damaging |
Het |
| Olfr543 |
A |
T |
7: 102,477,829 (GRCm38) |
Y14N |
probably damaging |
Het |
| Pik3c2b |
A |
T |
1: 133,075,809 (GRCm38) |
|
probably null |
Het |
| Pkn1 |
G |
T |
8: 83,681,008 (GRCm38) |
N463K |
probably damaging |
Het |
| Rab11fip3 |
C |
T |
17: 25,997,989 (GRCm38) |
S816N |
probably damaging |
Het |
| Rassf4 |
G |
T |
6: 116,640,257 (GRCm38) |
D262E |
probably damaging |
Het |
| Sec16a |
A |
G |
2: 26,421,372 (GRCm38) |
|
probably null |
Het |
| Sspo |
A |
G |
6: 48,468,494 (GRCm38) |
T2290A |
probably benign |
Het |
| Sumf1 |
A |
G |
6: 108,152,225 (GRCm38) |
|
probably null |
Het |
| Syne1 |
T |
C |
10: 5,229,248 (GRCm38) |
K4409E |
probably benign |
Het |
| Tmco4 |
T |
G |
4: 139,058,461 (GRCm38) |
L614R |
probably damaging |
Het |
| Tmem67 |
G |
T |
4: 12,070,592 (GRCm38) |
N245K |
probably damaging |
Het |
| Trim58 |
T |
C |
11: 58,651,312 (GRCm38) |
V366A |
probably benign |
Het |
| Vmn1r123 |
T |
C |
7: 21,162,989 (GRCm38) |
S269P |
probably damaging |
Het |
| Vmn2r91 |
T |
A |
17: 18,107,625 (GRCm38) |
S494T |
probably benign |
Het |
| Zbtb26 |
T |
A |
2: 37,436,875 (GRCm38) |
I50L |
possibly damaging |
Het |
|
| Other mutations in Ccdc150 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00712:Ccdc150
|
APN |
1 |
54,272,550 (GRCm38) |
splice site |
probably benign |
|
|
IGL00819:Ccdc150
|
APN |
1 |
54,263,573 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL01973:Ccdc150
|
APN |
1 |
54,300,488 (GRCm38) |
splice site |
probably null |
|
|
IGL02352:Ccdc150
|
APN |
1 |
54,272,521 (GRCm38) |
missense |
probably benign |
0.25 |
|
IGL02359:Ccdc150
|
APN |
1 |
54,272,521 (GRCm38) |
missense |
probably benign |
0.25 |
|
IGL02620:Ccdc150
|
APN |
1 |
54,263,545 (GRCm38) |
nonsense |
probably null |
|
|
IGL02673:Ccdc150
|
APN |
1 |
54,328,990 (GRCm38) |
missense |
probably benign |
0.09 |
|
IGL03148:Ccdc150
|
APN |
1 |
54,278,715 (GRCm38) |
missense |
possibly damaging |
0.68 |
|
IGL03185:Ccdc150
|
APN |
1 |
54,300,323 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL03014:Ccdc150
|
UTSW |
1 |
54,290,702 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0066:Ccdc150
|
UTSW |
1 |
54,356,691 (GRCm38) |
missense |
probably benign |
|
|
R0066:Ccdc150
|
UTSW |
1 |
54,356,691 (GRCm38) |
missense |
probably benign |
|
|
R0217:Ccdc150
|
UTSW |
1 |
54,300,430 (GRCm38) |
missense |
possibly damaging |
0.87 |
|
R0582:Ccdc150
|
UTSW |
1 |
54,329,511 (GRCm38) |
missense |
probably benign |
|
|
R0687:Ccdc150
|
UTSW |
1 |
54,285,631 (GRCm38) |
splice site |
probably null |
|
|
R0790:Ccdc150
|
UTSW |
1 |
54,277,776 (GRCm38) |
splice site |
probably benign |
|
|
R1146:Ccdc150
|
UTSW |
1 |
54,364,971 (GRCm38) |
splice site |
probably benign |
|
|
R1288:Ccdc150
|
UTSW |
1 |
54,364,458 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1763:Ccdc150
|
UTSW |
1 |
54,354,636 (GRCm38) |
missense |
probably benign |
0.42 |
|
R1855:Ccdc150
|
UTSW |
1 |
54,367,910 (GRCm38) |
intron |
probably benign |
|
|
R1957:Ccdc150
|
UTSW |
1 |
54,263,909 (GRCm38) |
missense |
probably benign |
0.00 |
|
R2180:Ccdc150
|
UTSW |
1 |
54,272,547 (GRCm38) |
critical splice donor site |
probably null |
|
|
R2226:Ccdc150
|
UTSW |
1 |
54,364,925 (GRCm38) |
missense |
probably null |
0.11 |
|
R3054:Ccdc150
|
UTSW |
1 |
54,288,842 (GRCm38) |
missense |
possibly damaging |
0.51 |
|
R3055:Ccdc150
|
UTSW |
1 |
54,288,842 (GRCm38) |
missense |
possibly damaging |
0.51 |
|
R3056:Ccdc150
|
UTSW |
1 |
54,288,842 (GRCm38) |
missense |
possibly damaging |
0.51 |
|
R3409:Ccdc150
|
UTSW |
1 |
54,356,773 (GRCm38) |
missense |
probably benign |
0.02 |
|
R3411:Ccdc150
|
UTSW |
1 |
54,356,773 (GRCm38) |
missense |
probably benign |
0.02 |
|
R3812:Ccdc150
|
UTSW |
1 |
54,368,310 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4031:Ccdc150
|
UTSW |
1 |
54,278,811 (GRCm38) |
missense |
probably benign |
0.31 |
|
R4356:Ccdc150
|
UTSW |
1 |
54,353,054 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4617:Ccdc150
|
UTSW |
1 |
54,355,754 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4757:Ccdc150
|
UTSW |
1 |
54,278,715 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R4957:Ccdc150
|
UTSW |
1 |
54,364,868 (GRCm38) |
intron |
probably benign |
|
|
R5028:Ccdc150
|
UTSW |
1 |
54,263,477 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5512:Ccdc150
|
UTSW |
1 |
54,354,647 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R5757:Ccdc150
|
UTSW |
1 |
54,263,620 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5943:Ccdc150
|
UTSW |
1 |
54,300,367 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5948:Ccdc150
|
UTSW |
1 |
54,277,714 (GRCm38) |
missense |
possibly damaging |
0.79 |
|
R6033:Ccdc150
|
UTSW |
1 |
54,285,628 (GRCm38) |
critical splice donor site |
probably null |
|
|
R6033:Ccdc150
|
UTSW |
1 |
54,285,628 (GRCm38) |
critical splice donor site |
probably null |
|
|
R6065:Ccdc150
|
UTSW |
1 |
54,263,599 (GRCm38) |
missense |
possibly damaging |
0.90 |
|
R6390:Ccdc150
|
UTSW |
1 |
54,368,017 (GRCm38) |
missense |
probably benign |
0.01 |
|
R6399:Ccdc150
|
UTSW |
1 |
54,263,957 (GRCm38) |
splice site |
probably null |
|
|
R6988:Ccdc150
|
UTSW |
1 |
54,355,709 (GRCm38) |
nonsense |
probably null |
|
|
R7248:Ccdc150
|
UTSW |
1 |
54,304,898 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7319:Ccdc150
|
UTSW |
1 |
54,263,337 (GRCm38) |
splice site |
probably null |
|
|
R7322:Ccdc150
|
UTSW |
1 |
54,259,966 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7366:Ccdc150
|
UTSW |
1 |
54,300,382 (GRCm38) |
nonsense |
probably null |
|
|
R7647:Ccdc150
|
UTSW |
1 |
54,356,704 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8002:Ccdc150
|
UTSW |
1 |
54,272,497 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R8201:Ccdc150
|
UTSW |
1 |
54,329,487 (GRCm38) |
missense |
probably benign |
0.10 |
|
R8688:Ccdc150
|
UTSW |
1 |
54,367,973 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8719:Ccdc150
|
UTSW |
1 |
54,263,509 (GRCm38) |
missense |
probably benign |
0.00 |
|
R8963:Ccdc150
|
UTSW |
1 |
54,272,482 (GRCm38) |
missense |
probably benign |
0.14 |
|
R9178:Ccdc150
|
UTSW |
1 |
54,272,485 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R9200:Ccdc150
|
UTSW |
1 |
54,260,038 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9332:Ccdc150
|
UTSW |
1 |
54,277,751 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R9367:Ccdc150
|
UTSW |
1 |
54,285,601 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9416:Ccdc150
|
UTSW |
1 |
54,278,831 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R9430:Ccdc150
|
UTSW |
1 |
54,281,771 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9576:Ccdc150
|
UTSW |
1 |
54,368,385 (GRCm38) |
nonsense |
probably null |
|
|
R9747:Ccdc150
|
UTSW |
1 |
54,259,948 (GRCm38) |
nonsense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- TCTACTCGACAACTGCTGGG -3'
(R):5'- TGTGGCCATTTCACACAACAC -3'
Sequencing Primer
(F):5'- CACCTTTCCCAAACTATGTAGATC -3'
(R):5'- TTCCTGAAAACAGCAAATACCATGTG -3'
|
| Posted On |
2020-09-15 |
Incidental Mutation