Mutagenetix > Incidental Mutation

Incidental Mutation 'R7993:Or8b8'

651873

Institutional Source

Beutler Lab

Gene Symbol

Or8b8

Ensembl Gene

ENSMUSG00000066748

Gene Name

olfactory receptor family 8 subfamily B member 8

Synonyms

GA_x6K02T2PVTD-31578734-31579666, Olfr145, K21, MOR161-6

MMRRC Submission

046034-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.141) question?

Stock #

R7993 (G1)

Quality Score

164.009

Status

Validated

Chromosome

Chromosomal Location

37808020-37814815 bp(+) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

A to T at 37808633 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000150284 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086062] [ENSMUST00000213688]

AlphaFold

Q60882

Predicted Effect

probably benign
Transcript: ENSMUST00000086062

SMART Domains

Protein: ENSMUSP00000083229
Gene: ENSMUSG00000066748

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	307	4e-51	PFAM
Pfam:7tm_1	40	289	1.4e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213688

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

100% (69/69)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc2	T	A	19: 43,803,231 (GRCm39)	V689D	possibly damaging	Het
Adamts17	T	C	7: 66,499,612 (GRCm39)	V53A	possibly damaging	Het
Adcy9	T	C	16: 4,235,866 (GRCm39)	N515S	probably damaging	Het
Aoc1l3	T	A	6: 48,964,542 (GRCm39)	N183K	possibly damaging	Het
Atp1a2	A	G	1: 172,118,878 (GRCm39)	V88A	possibly damaging	Het
Atp1a3	T	C	7: 24,700,406 (GRCm39)		probably null	Het
Baz2a	T	C	10: 127,961,491 (GRCm39)	F1706L	probably benign	Het
Cacnb2	A	C	2: 14,968,731 (GRCm39)	N199T	probably benign	Het
Caskin1	T	A	17: 24,718,279 (GRCm39)	Y296*	probably null	Het
Cemip	C	A	7: 83,613,383 (GRCm39)	G605V	probably damaging	Het
Ciart	T	A	3: 95,786,206 (GRCm39)	K290*	probably null	Het
Cideb	G	T	14: 55,995,899 (GRCm39)		probably benign	Het
Copg2	A	T	6: 30,793,097 (GRCm39)	Y413N	probably damaging	Het
Csrp1	T	A	1: 135,674,453 (GRCm39)		probably null	Het
Cyp2j8	C	T	4: 96,335,456 (GRCm39)		probably null	Het
Dcbld1	T	A	10: 52,137,884 (GRCm39)	Y49*	probably null	Het
Dpep1	T	C	8: 123,927,460 (GRCm39)	V338A	possibly damaging	Het
Exoc2	A	G	13: 31,090,713 (GRCm39)		probably null	Het
Fcho2	A	T	13: 98,888,524 (GRCm39)		probably null	Het
Foxd3	C	T	4: 99,544,841 (GRCm39)		probably benign	Het
Gabbr2	C	T	4: 46,736,349 (GRCm39)		probably null	Het
Gipc3	T	C	10: 81,173,805 (GRCm39)	D269G	probably damaging	Het
Gm4861	G	A	3: 137,256,417 (GRCm39)	T63M	probably damaging	Het
Hmga1b	C	A	11: 120,653,833 (GRCm39)	A40E	probably damaging	Het
Hydin	T	A	8: 111,306,264 (GRCm39)	M3889K	probably benign	Het
Inhca	T	A	9: 103,140,332 (GRCm39)	K462N	probably benign	Het
Klk14	T	A	7: 43,344,367 (GRCm39)	M226K	probably benign	Het
Liph	T	G	16: 21,777,562 (GRCm39)	M413L	probably benign	Het
Lrrc37a	T	C	11: 103,348,787 (GRCm39)	D2636G	unknown	Het
Lrrk1	G	A	7: 65,912,202 (GRCm39)	T1786M	probably benign	Het
Map1a	A	G	2: 121,135,057 (GRCm39)	S1958G	possibly damaging	Het
Msto1	A	G	3: 88,817,481 (GRCm39)	F484S	probably benign	Het
Muc2	C	T	7: 141,308,173 (GRCm39)	H890Y		Het
Nectin3	T	C	16: 46,279,184 (GRCm39)	I265V	probably benign	Het
Nectin4	C	T	1: 171,211,322 (GRCm39)	T282I	probably damaging	Het
Nfat5	T	A	8: 108,082,134 (GRCm39)		probably null	Het
Nsmaf	T	C	4: 6,398,647 (GRCm39)	D819G	probably benign	Het
Pcdhb1	A	T	18: 37,400,044 (GRCm39)	D665V	probably damaging	Het
Pkd1l1	T	C	11: 8,895,262 (GRCm39)	D616G		Het
Plppr2	A	T	9: 21,858,258 (GRCm39)	H286L	probably damaging	Het
Polrmt	T	C	10: 79,572,085 (GRCm39)	T1203A	probably damaging	Het
Ppm1d	T	A	11: 85,217,777 (GRCm39)	V180E	probably damaging	Het
Ppp1r18	T	A	17: 36,184,718 (GRCm39)	I551N	probably benign	Het
Psg28	C	T	7: 18,160,401 (GRCm39)	C265Y	possibly damaging	Het
Psmc1	A	G	12: 100,081,824 (GRCm39)	D142G	probably benign	Het
Rabgap1l	G	T	1: 160,528,424 (GRCm39)	A394E	probably damaging	Het
Rbm48	G	A	5: 3,640,470 (GRCm39)	P303L	probably benign	Het
Rnf167	T	C	11: 70,540,821 (GRCm39)	V185A	probably benign	Het
Ros1	T	A	10: 51,999,443 (GRCm39)	Q1169L	probably benign	Het
Rrp1b	A	G	17: 32,277,541 (GRCm39)	D607G	probably damaging	Het
Scamp1	A	G	13: 94,366,294 (GRCm39)	Y134H	probably damaging	Het
Scfd1	G	A	12: 51,492,490 (GRCm39)	E600K	probably damaging	Het
Scn3b	A	G	9: 40,193,840 (GRCm39)	E189G	possibly damaging	Het
Scn4b	G	T	9: 45,059,007 (GRCm39)	V93L	probably benign	Het
Serpina3i	A	T	12: 104,231,407 (GRCm39)	T15S	possibly damaging	Het
Slc2a13	A	T	15: 91,296,356 (GRCm39)	C319*	probably null	Het
Tdrd1	A	G	19: 56,854,437 (GRCm39)		probably null	Het
Tep1	T	A	14: 51,067,710 (GRCm39)	I2169F	probably benign	Het
Tm4sf19	A	G	16: 32,226,458 (GRCm39)	D124G	possibly damaging	Het
Trim2	T	C	3: 84,098,026 (GRCm39)	Y434C	probably damaging	Het
Usp34	T	A	11: 23,327,622 (GRCm39)	S1056T		Het
Vmn1r230	T	A	17: 21,067,312 (GRCm39)	M167K	probably benign	Het
Vmn2r14	T	A	5: 109,363,862 (GRCm39)	M685L	probably benign	Het
Zfhx4	T	A	3: 5,478,047 (GRCm39)	V3554D	probably damaging	Het
Zfp1010	C	T	2: 176,957,015 (GRCm39)	G161D	probably damaging	Het
Zfp458	A	G	13: 67,405,234 (GRCm39)	S402P	probably damaging	Het
Zfp462	C	A	4: 55,011,907 (GRCm39)	A1291E	probably damaging	Het
Zmat5	G	A	11: 4,672,379 (GRCm39)		probably benign	Het
Znfx1	C	A	2: 166,897,857 (GRCm39)	E356*	probably null	Het
Zswim5	A	G	4: 116,808,291 (GRCm39)	T292A	probably benign	Het

Other mutations in Or8b8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01362:Or8b8	APN	9	37,809,359 (GRCm39)	missense	probably benign	0.13
IGL01586:Or8b8	APN	9	37,809,272 (GRCm39)	missense	possibly damaging	0.73
IGL02185:Or8b8	APN	9	37,809,531 (GRCm39)	missense	probably damaging	1.00
IGL02895:Or8b8	APN	9	37,809,278 (GRCm39)	missense	probably benign	0.01
IGL02956:Or8b8	APN	9	37,809,404 (GRCm39)	missense	probably damaging	1.00
R0391:Or8b8	UTSW	9	37,809,138 (GRCm39)	missense	probably benign	0.31
R0513:Or8b8	UTSW	9	37,809,351 (GRCm39)	missense	probably damaging	1.00
R4600:Or8b8	UTSW	9	37,809,622 (GRCm39)	missense	probably benign
R4610:Or8b8	UTSW	9	37,809,622 (GRCm39)	missense	probably benign
R4611:Or8b8	UTSW	9	37,809,622 (GRCm39)	missense	probably benign
R4982:Or8b8	UTSW	9	37,808,811 (GRCm39)	missense	probably damaging	1.00
R5574:Or8b8	UTSW	9	37,808,877 (GRCm39)	missense	probably damaging	1.00
R5608:Or8b8	UTSW	9	37,809,078 (GRCm39)	missense	probably damaging	0.98
R5688:Or8b8	UTSW	9	37,809,359 (GRCm39)	missense	possibly damaging	0.91
R5906:Or8b8	UTSW	9	37,809,174 (GRCm39)	missense	probably damaging	1.00
R6286:Or8b8	UTSW	9	37,809,074 (GRCm39)	missense	probably damaging	0.99
R7138:Or8b8	UTSW	9	37,809,360 (GRCm39)	missense	probably damaging	0.99
R7145:Or8b8	UTSW	9	37,808,859 (GRCm39)	missense	probably benign	0.01
R8046:Or8b8	UTSW	9	37,808,685 (GRCm39)	splice site	probably benign
R8185:Or8b8	UTSW	9	37,809,531 (GRCm39)	missense	probably damaging	1.00
R9276:Or8b8	UTSW	9	37,809,415 (GRCm39)	missense	probably benign	0.04
R9352:Or8b8	UTSW	9	37,808,712 (GRCm39)	missense	probably benign	0.00
X0012:Or8b8	UTSW	9	37,809,624 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACTTGAGAGTCCCCTCACTG -3'
(R):5'- ATCAAGCCCAGGTTTCCCAC -3'

Sequencing Primer
(F):5'- ACTGGCTCTCTGGGATCC -3'
(R):5'- ATGGTGACCATGTAGAAACCTAG -3'

Posted On

2020-09-15