Mutagenetix > Incidental Mutation

Incidental Mutation 'R8206:Zfp677'

652102

Institutional Source

Beutler Lab

Gene Symbol

Zfp677

Ensembl Gene

ENSMUSG00000062743

Gene Name

zinc finger protein 677

Synonyms

A830058L05Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R8206 (G1)

Quality Score

211.009

Status

Validated

Chromosome

Chromosomal Location

21383748-21399265 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 21392455 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000125295 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056107] [ENSMUST00000162659]

AlphaFold

Q6PEP4

Predicted Effect

probably null
Transcript: ENSMUST00000056107

SMART Domains

Protein: ENSMUSP00000052667
Gene: ENSMUSG00000062743

Domain	Start	End	E-Value	Type
KRAB	13	75	1.11e-21	SMART
ZnF_C2H2	185	207	2.95e-3	SMART
ZnF_C2H2	213	235	3.95e-4	SMART
ZnF_C2H2	241	263	2.09e-3	SMART
ZnF_C2H2	269	291	6.42e-4	SMART
ZnF_C2H2	297	319	5.5e-3	SMART
ZnF_C2H2	325	347	1.98e-4	SMART
ZnF_C2H2	353	375	1.98e-4	SMART
ZnF_C2H2	381	403	1.47e-3	SMART
ZnF_C2H2	409	431	1.28e-3	SMART
ZnF_C2H2	437	459	3.95e-4	SMART
ZnF_C2H2	465	487	1.04e-3	SMART
ZnF_C2H2	493	515	8.47e-4	SMART
ZnF_C2H2	521	543	7.49e-5	SMART
ZnF_C2H2	549	571	1.18e-2	SMART
ZnF_C2H2	577	599	6.08e-5	SMART
ZnF_C2H2	610	632	4.17e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000162659

SMART Domains

Protein: ENSMUSP00000125295
Gene: ENSMUSG00000062743

Domain	Start	End	E-Value	Type
KRAB	13	75	1.11e-21	SMART
Pfam:zf-H2C2_2	118	140	2.9e-5	PFAM
ZnF_C2H2	185	207	2.95e-3	SMART
ZnF_C2H2	213	235	3.95e-4	SMART
ZnF_C2H2	241	263	2.09e-3	SMART
ZnF_C2H2	269	291	6.42e-4	SMART
ZnF_C2H2	297	319	5.5e-3	SMART
ZnF_C2H2	325	347	1.98e-4	SMART
ZnF_C2H2	353	375	1.98e-4	SMART
ZnF_C2H2	381	403	1.47e-3	SMART
ZnF_C2H2	409	431	1.28e-3	SMART
ZnF_C2H2	437	459	3.95e-4	SMART
ZnF_C2H2	465	487	1.04e-3	SMART
ZnF_C2H2	493	515	8.47e-4	SMART
ZnF_C2H2	521	543	7.49e-5	SMART
ZnF_C2H2	549	571	1.18e-2	SMART
ZnF_C2H2	577	599	6.08e-5	SMART
ZnF_C2H2	610	632	4.17e-3	SMART

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9230110F15Rik	A	T	9: 35,839,423	F34L	possibly damaging	Het
Ano4	A	G	10: 89,025,096	Y342H	probably damaging	Het
Aqp4	T	A	18: 15,393,659	D255V	possibly damaging	Het
Arhgap26	C	A	18: 39,306,750	S247*	probably null	Het
Arid4a	A	G	12: 71,086,587	D1154G	probably damaging	Het
Atpaf2	A	G	11: 60,404,478	I182T	probably damaging	Het
Cacna1i	C	A	15: 80,389,815		probably null	Het
Ccdc38	A	G	10: 93,563,284	S205G	probably damaging	Het
Cep63	A	G	9: 102,621,271		probably benign	Het
Cyp24a1	T	C	2: 170,491,669	T255A	possibly damaging	Het
Dlg5	A	G	14: 24,160,268	S787P	possibly damaging	Het
Dnah6	A	T	6: 73,037,566	C3679*	probably null	Het
Dpy19l3	T	C	7: 35,729,730	Y95C	probably damaging	Het
Erc2	G	A	14: 28,303,015		probably null	Het
Ezh2	A	T	6: 47,532,900		probably null	Het
Fgfr1	A	G	8: 25,570,242	T463A	probably damaging	Het
Flvcr2	GTAGTGTATA	GTA	12: 85,803,148		probably null	Het
Fsip2	T	C	2: 82,990,464	S5514P	possibly damaging	Het
Glrb	A	G	3: 80,851,066	Y347H	probably damaging	Het
Gm14325	C	A	2: 177,832,974	C105F	probably damaging	Het
Hgsnat	T	C	8: 25,954,637	T428A	probably damaging	Het
Ighv1-76	T	C	12: 115,848,314	M1V	probably null	Het
Inppl1	A	G	7: 101,823,576	I1207T	possibly damaging	Het
Kmt2c	T	A	5: 25,314,539	Q2191L	probably damaging	Het
Krt79	T	A	15: 101,940,270		probably null	Het
Mast4	G	A	13: 102,735,739	L2374F	probably damaging	Het
Mgam	A	G	6: 40,680,235	N951S	probably benign	Het
Myl10	G	C	5: 136,697,971	V70L	probably benign	Het
Naip6	A	T	13: 100,294,836	C1164*	probably null	Het
Nfatc2ip	G	T	7: 126,390,734	D189E	probably damaging	Het
Nrp1	A	G	8: 128,457,957	D361G	probably damaging	Het
Nrp2	T	C	1: 62,747,215	I293T	probably damaging	Het
Pde3a	T	C	6: 141,487,885	V831A	probably damaging	Het
Pirb	A	G	7: 3,712,906		probably null	Het
Plch1	G	T	3: 63,702,626		probably null	Het
Plekhh2	A	G	17: 84,590,849	T973A	possibly damaging	Het
Ppp1r35	T	A	5: 137,780,034	I97K	unknown	Het
Ppp1r3c	C	T	19: 36,733,446	G308E	probably benign	Het
Prss12	G	A	3: 123,464,962		probably null	Het
Rad51b	A	G	12: 79,314,941	D142G	probably damaging	Het
Slc13a3	C	T	2: 165,406,825	G553D	probably damaging	Het
Spata31d1d	A	C	13: 59,731,530	V64G	probably benign	Het
Srp68	G	A	11: 116,273,983	R42C	probably damaging	Het
Syne2	T	C	12: 76,015,591	V4229A	probably benign	Het
Tas2r144	G	A	6: 42,215,391	V22M	probably damaging	Het
Tcf7l1	A	G	6: 72,627,412	L583P	probably damaging	Het
Tdgf1	G	A	9: 110,944,284		probably benign	Het
Tdrd3	A	G	14: 87,511,778	D708G	probably benign	Het
Tns4	A	T	11: 99,085,801	L98Q	probably damaging	Het

Other mutations in Zfp677

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00481:Zfp677	APN	17	21397668	missense	probably benign	0.33
IGL01973:Zfp677	APN	17	21396907	missense	probably damaging	1.00
IGL02206:Zfp677	APN	17	21393237	missense	probably damaging	1.00
IGL03240:Zfp677	APN	17	21396873	missense	probably damaging	0.99
IGL03409:Zfp677	APN	17	21396845	missense	probably damaging	1.00
R0622:Zfp677	UTSW	17	21397700	missense	probably benign	0.04
R0972:Zfp677	UTSW	17	21398310	missense	probably damaging	1.00
R1519:Zfp677	UTSW	17	21397237	missense	possibly damaging	0.91
R2155:Zfp677	UTSW	17	21397708	missense	probably benign	0.01
R2316:Zfp677	UTSW	17	21397320	missense	probably benign	0.38
R2866:Zfp677	UTSW	17	21397256	nonsense	probably null
R2989:Zfp677	UTSW	17	21396852	missense	probably benign	0.11
R3955:Zfp677	UTSW	17	21397817	missense	possibly damaging	0.95
R4075:Zfp677	UTSW	17	21398159	missense	probably damaging	1.00
R4134:Zfp677	UTSW	17	21397781	missense	probably benign	0.01
R4229:Zfp677	UTSW	17	21398282	missense	probably damaging	1.00
R4729:Zfp677	UTSW	17	21397418	missense	possibly damaging	0.51
R4843:Zfp677	UTSW	17	21392526	missense	probably benign	0.23
R5023:Zfp677	UTSW	17	21397794	missense	probably damaging	1.00
R5316:Zfp677	UTSW	17	21397148	missense	probably damaging	0.99
R5420:Zfp677	UTSW	17	21397913	missense	probably damaging	1.00
R5694:Zfp677	UTSW	17	21397759	missense	probably damaging	0.99
R5837:Zfp677	UTSW	17	21397386	missense	probably damaging	1.00
R5888:Zfp677	UTSW	17	21398258	missense	probably damaging	1.00
R6007:Zfp677	UTSW	17	21397656	missense	probably damaging	1.00
R6119:Zfp677	UTSW	17	21397808	missense	possibly damaging	0.55
R6190:Zfp677	UTSW	17	21397268	missense	possibly damaging	0.91
R6518:Zfp677	UTSW	17	21398130	missense	probably damaging	1.00
R7198:Zfp677	UTSW	17	21398417	missense	probably damaging	1.00
R7391:Zfp677	UTSW	17	21398391	missense	possibly damaging	0.56
R7801:Zfp677	UTSW	17	21398015	missense	probably damaging	1.00
R7808:Zfp677	UTSW	17	21397385	missense	probably damaging	1.00
R8202:Zfp677	UTSW	17	21393273	missense	probably damaging	1.00
R8885:Zfp677	UTSW	17	21398088	missense	probably benign
R8965:Zfp677	UTSW	17	21396893	missense	probably damaging	1.00
R9062:Zfp677	UTSW	17	21392553	critical splice donor site	probably null
R9167:Zfp677	UTSW	17	21393198	missense	probably damaging	1.00
R9371:Zfp677	UTSW	17	21397791	missense	probably damaging	1.00
R9638:Zfp677	UTSW	17	21397794	missense	probably damaging	1.00
R9752:Zfp677	UTSW	17	21398249	missense	probably damaging	1.00
RF003:Zfp677	UTSW	17	21397442	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCCATGGTGCTTTTCTGTC -3'
(R):5'- CTCCAGAGTTTCTGTTCTCAATGAC -3'

Sequencing Primer
(F):5'- ATGGTGCTTTTCTGTCCAAAC -3'
(R):5'- AGAGTTTCTGTTCTCAATGACTACTC -3'

Posted On

2020-10-14