Mutagenetix > Incidental Mutation

Incidental Mutation 'R8405:Or56b2j'

652351

Institutional Source

Beutler Lab

Gene Symbol

Or56b2j

Ensembl Gene

ENSMUSG00000073919

Gene Name

olfactory receptor family 56 subfamily B member 2J

Synonyms

MOR40-12, Olfr663, GA_x6K02T2PBJ9-7331927-7332961

MMRRC Submission

067879-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.504) question?

Stock #

R8405 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

104352776-104353734 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 104353208 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 145 (S145T)

Ref Sequence

ENSEMBL: ENSMUSP00000147953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000209914]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000209914
AA Change: S145T

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

98% (49/50)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acadm	T	C	3: 153,635,165 (GRCm39)		probably benign	Het
Anxa3	C	A	5: 96,978,295 (GRCm39)	D226E	probably benign	Het
Anxa8	T	C	14: 33,819,881 (GRCm39)	L290P	probably damaging	Het
Brd4	C	A	17: 32,448,505 (GRCm39)	K57N	unknown	Het
Cpne8	T	A	15: 90,456,235 (GRCm39)	Q176L	possibly damaging	Het
Csf2rb2	T	C	15: 78,172,093 (GRCm39)	D439G	possibly damaging	Het
Csmd3	A	G	15: 47,619,379 (GRCm39)	S1832P	probably damaging	Het
Cux1	A	G	5: 136,304,241 (GRCm39)	S1320P	possibly damaging	Het
Cyp2t4	A	T	7: 26,856,894 (GRCm39)		probably null	Het
Dnah2	A	T	11: 69,349,289 (GRCm39)	L2449Q	probably damaging	Het
Dock1	A	G	7: 134,379,192 (GRCm39)	M640V	probably benign	Het
Efcab3	G	A	11: 104,612,024 (GRCm39)	S622N	probably benign	Het
Ergic1	T	A	17: 26,874,096 (GRCm39)	I274N	probably damaging	Het
Fat3	T	C	9: 15,907,167 (GRCm39)	D2945G	probably damaging	Het
Fscb	T	C	12: 64,520,278 (GRCm39)	E396G	possibly damaging	Het
Gab1	T	A	8: 81,501,594 (GRCm39)	R581*	probably null	Het
Gabarapl1	A	G	6: 129,514,497 (GRCm39)	D45G	probably null	Het
Gfap	C	A	11: 102,782,255 (GRCm39)	Q418H	probably benign	Het
Gfap	T	A	11: 102,782,256 (GRCm39)	Q418L	probably benign	Het
Hectd1	T	A	12: 51,874,178 (GRCm39)	M33L	probably benign	Het
Kcnh7	T	A	2: 62,533,446 (GRCm39)	K1144N	probably benign	Het
Lrit3	C	T	3: 129,582,301 (GRCm39)	G562D	probably benign	Het
Mab21l1	A	T	3: 55,690,659 (GRCm39)	Q82L	probably damaging	Het
Naip6	C	T	13: 100,436,784 (GRCm39)	A580T	possibly damaging	Het
Nav1	T	C	1: 135,382,508 (GRCm39)	K1258R	unknown	Het
Nbas	C	A	12: 13,329,394 (GRCm39)	A113E	probably damaging	Het
Nlrp1b	A	T	11: 71,073,356 (GRCm39)	D162E	possibly damaging	Het
Nr2c1	A	T	10: 94,031,155 (GRCm39)	H572L	probably benign	Het
Or52s1	A	T	7: 102,861,408 (GRCm39)	I114F	probably benign	Het
Pcdha12	T	A	18: 37,154,250 (GRCm39)	V323E	possibly damaging	Het
Pgm5	T	A	19: 24,705,106 (GRCm39)	H469L	probably benign	Het
Phf8-ps	C	A	17: 33,286,038 (GRCm39)	A255S	probably benign	Het
Pja2	T	C	17: 64,616,505 (GRCm39)	E130G	possibly damaging	Het
Ppp2r2b	G	C	18: 42,778,805 (GRCm39)	D443E	probably benign	Het
Prdm9	T	A	17: 15,764,456 (GRCm39)	T775S	probably benign	Het
Rbm6	A	G	9: 107,730,016 (GRCm39)	S211P	probably benign	Het
Rgl2	C	T	17: 34,152,698 (GRCm39)	Q408*	probably null	Het
Rgs14	C	T	13: 55,530,962 (GRCm39)	R389W	probably damaging	Het
Rtcb	T	C	10: 85,793,534 (GRCm39)	D13G	probably benign	Het
Slc23a4	C	T	6: 34,923,145 (GRCm39)	C280Y	probably benign	Het
Slc7a5	G	T	8: 122,613,661 (GRCm39)	T297K	possibly damaging	Het
Spef2	A	G	15: 9,612,643 (GRCm39)	V1229A	probably benign	Het
Stag3	A	C	5: 138,302,914 (GRCm39)	T1064P	probably damaging	Het
Tnnt1	A	G	7: 4,510,592 (GRCm39)	S211P	probably damaging	Het
Trim26	A	G	17: 37,167,095 (GRCm39)	D262G	possibly damaging	Het
Trim30a	A	T	7: 104,060,749 (GRCm39)	Y342*	probably null	Het
Trpm7	T	C	2: 126,658,755 (GRCm39)	I1134V	probably benign	Het
Tsc22d1	G	A	14: 76,655,734 (GRCm39)	G738S	probably damaging	Het
Usp39	A	G	6: 72,305,521 (GRCm39)	F421L	probably damaging	Het
Vmn2r97	G	A	17: 19,134,802 (GRCm39)		probably null	Het
Zfat	T	C	15: 68,018,410 (GRCm39)	Y968C	probably damaging	Het

Other mutations in Or56b2j

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R3789:Or56b2j	UTSW	7	104,353,156 (GRCm39)	missense	probably damaging	1.00
R4884:Or56b2j	UTSW	7	104,353,068 (GRCm39)	missense	probably damaging	1.00
R5227:Or56b2j	UTSW	7	104,353,529 (GRCm39)	missense	possibly damaging	0.49
R5372:Or56b2j	UTSW	7	104,353,002 (GRCm39)	missense	probably benign	0.24
R5416:Or56b2j	UTSW	7	104,352,923 (GRCm39)	missense	probably benign	0.18
R5699:Or56b2j	UTSW	7	104,353,200 (GRCm39)	missense	probably damaging	1.00
R6207:Or56b2j	UTSW	7	104,352,818 (GRCm39)	missense	probably damaging	0.96
R6715:Or56b2j	UTSW	7	104,353,163 (GRCm39)	missense	possibly damaging	0.64
R7426:Or56b2j	UTSW	7	104,352,796 (GRCm39)	missense	probably benign	0.00
R7994:Or56b2j	UTSW	7	104,353,635 (GRCm39)	missense	probably damaging	1.00
R8746:Or56b2j	UTSW	7	104,353,067 (GRCm39)	missense	probably damaging	1.00
R9443:Or56b2j	UTSW	7	104,352,821 (GRCm39)	missense	probably benign	0.10
Z1088:Or56b2j	UTSW	7	104,353,700 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TATCATGCCCAAGATTTTAGCCATC -3'
(R):5'- ACTACCCATGACTGCCCATG -3'

Sequencing Primer
(F):5'- CCATCTTATGGTTCAATGCTAAGGC -3'
(R):5'- ATGACTGCCCATGCCAGGAAG -3'

Posted On

2020-10-20