Mutagenetix > Incidental Mutation

Incidental Mutation 'R8407:Zfp957'

652495

Institutional Source

Beutler Lab

Gene Symbol

Zfp957

Ensembl Gene

ENSMUSG00000071262

Gene Name

zinc finger protein 957

Synonyms

AU017455

MMRRC Submission

067814-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.365) question?

Stock #

R8407 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

79449795-79484807 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 79451352 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 149 (V149A)

Ref Sequence

ENSEMBL: ENSMUSP00000039470 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040802] [ENSMUST00000161649]

AlphaFold

Q3UT76

Predicted Effect

possibly damaging
Transcript: ENSMUST00000040802
AA Change: V149A

PolyPhen 2 Score 0.911 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000039470
Gene: ENSMUSG00000071262
AA Change: V149A

Domain	Start	End	E-Value	Type
low complexity region	3	19	N/A	INTRINSIC
low complexity region	29	53	N/A	INTRINSIC
low complexity region	147	161	N/A	INTRINSIC
low complexity region	220	231	N/A	INTRINSIC
AT_hook	255	267	4.76e0	SMART
AT_hook	298	310	4.76e0	SMART
AT_hook	348	360	2.48e0	SMART
low complexity region	390	398	N/A	INTRINSIC
AT_hook	422	434	5.38e0	SMART
PHD	507	554	1.18e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000161649
AA Change: V149A

PolyPhen 2 Score 0.911 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000124930
Gene: ENSMUSG00000071262
AA Change: V149A

Domain	Start	End	E-Value	Type
low complexity region	3	19	N/A	INTRINSIC
low complexity region	29	53	N/A	INTRINSIC
low complexity region	147	161	N/A	INTRINSIC
low complexity region	220	231	N/A	INTRINSIC
AT_hook	255	267	4.76e0	SMART
AT_hook	298	310	4.76e0	SMART
AT_hook	348	360	2.48e0	SMART
low complexity region	390	398	N/A	INTRINSIC
AT_hook	422	434	5.38e0	SMART
PHD	507	554	1.18e-6	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acly	T	C	11: 100,384,897 (GRCm39)	I629V	possibly damaging	Het
Ahnak	C	T	19: 8,993,037 (GRCm39)	P4774S	probably benign	Het
Arhgef12	C	T	9: 42,937,475 (GRCm39)		probably null	Het
BC048562	T	A	9: 108,315,631 (GRCm39)	S12R	possibly damaging	Het
Calhm2	G	A	19: 47,098,755 (GRCm39)	Q310*	probably null	Het
Celsr3	A	G	9: 108,706,256 (GRCm39)	E913G	probably damaging	Het
Cep68	T	C	11: 20,190,446 (GRCm39)	S189G	possibly damaging	Het
Cilp	C	A	9: 65,181,898 (GRCm39)	P336T	probably damaging	Het
Cnot4	A	G	6: 35,033,154 (GRCm39)	S288P	probably benign	Het
Cst13	T	C	2: 148,665,124 (GRCm39)	S40P	probably damaging	Het
Cyp4f37	A	T	17: 32,853,158 (GRCm39)	D374V	probably damaging	Het
Ddit3	A	G	10: 127,131,318 (GRCm39)	T37A	probably benign	Het
Dnah2	T	C	11: 69,350,104 (GRCm39)	N2343S	probably benign	Het
Emp3	G	A	7: 45,569,445 (GRCm39)	P32L	probably damaging	Het
Esyt1	G	T	10: 128,347,796 (GRCm39)	L965M	probably damaging	Het
Fbxw28	T	A	9: 109,155,269 (GRCm39)	I406L	probably benign	Het
Fgf10	A	G	13: 118,851,938 (GRCm39)	T7A	possibly damaging	Het
Frs3	A	T	17: 48,009,552 (GRCm39)	D11V	probably damaging	Het
Glmn	A	G	5: 107,718,057 (GRCm39)	S287P	probably benign	Het
Glyctk	C	T	9: 106,033,141 (GRCm39)	A291T	probably benign	Het
H2bc12	G	T	13: 22,220,217 (GRCm39)	G54V	probably damaging	Het
Ibtk	A	T	9: 85,603,119 (GRCm39)	F629I	possibly damaging	Het
Kcnh8	C	A	17: 53,212,101 (GRCm39)	A633E	probably damaging	Het
Kif24	T	C	4: 41,394,488 (GRCm39)	N929S	probably benign	Het
Ldlrap1	T	C	4: 134,484,736 (GRCm39)	K86R	probably damaging	Het
Lfng	G	A	5: 140,598,981 (GRCm39)	E297K	probably damaging	Het
Lmod1	C	A	1: 135,292,734 (GRCm39)	P530T	possibly damaging	Het
Lmod1	A	G	1: 135,291,763 (GRCm39)	K206R	probably benign	Het
Lnp1	A	T	16: 56,748,251 (GRCm39)	S14T	probably benign	Het
Map3k6	T	A	4: 132,974,904 (GRCm39)	Y646N	possibly damaging	Het
Mapk14	G	A	17: 28,963,983 (GRCm39)	V290I	probably benign	Het
Mrpl22	C	A	11: 58,066,156 (GRCm39)	Y83*	probably null	Het
Myh6	T	C	14: 55,201,388 (GRCm39)	N104D	probably benign	Het
Nalcn	T	A	14: 123,554,683 (GRCm39)	M903L	probably damaging	Het
Nfat5	C	T	8: 108,094,047 (GRCm39)	Q763*	probably null	Het
Or5w19	A	C	2: 87,698,437 (GRCm39)	Y34S	probably damaging	Het
Plin1	T	C	7: 79,373,051 (GRCm39)	D306G	probably benign	Het
Ppp1r12a	T	A	10: 108,076,042 (GRCm39)		probably null	Het
Prelid1	T	A	13: 55,470,672 (GRCm39)	H33Q	probably damaging	Het
Prkcz	C	T	4: 155,352,673 (GRCm39)	A485T	probably damaging	Het
Ptch1	T	C	13: 63,662,057 (GRCm39)	E1169G	probably null	Het
Rps19	C	A	7: 24,588,517 (GRCm39)	T181K	unknown	Het
Skic2	G	A	17: 35,060,103 (GRCm39)	A889V	probably benign	Het
Slc22a3	A	C	17: 12,640,368 (GRCm39)	C538G	probably benign	Het
Slc2a10	T	A	2: 165,356,787 (GRCm39)	F149Y	possibly damaging	Het
Smarcal1	A	G	1: 72,640,554 (GRCm39)	I516M	probably benign	Het
Smarcc2	T	A	10: 128,318,190 (GRCm39)	W601R	probably damaging	Het
Son	AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	16: 91,457,222 (GRCm39)		probably benign	Het
Srp68	A	G	11: 116,143,589 (GRCm39)	S369P	probably benign	Het
Tex2	C	T	11: 106,459,221 (GRCm39)	E70K	probably damaging	Het
Ticam2	A	G	18: 46,693,590 (GRCm39)	S166P	probably damaging	Het
Trpv5	A	G	6: 41,652,272 (GRCm39)	S138P	probably benign	Het
Ttll8	C	T	15: 88,798,741 (GRCm39)	V665I	probably benign	Het
Vmn2r81	T	C	10: 79,104,028 (GRCm39)	L217P	possibly damaging	Het
Zbtb4	T	C	11: 69,669,101 (GRCm39)	V608A	probably benign	Het

Other mutations in Zfp957

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00672:Zfp957	APN	14	79,450,838 (GRCm39)	missense	unknown
IGL01646:Zfp957	APN	14	79,451,331 (GRCm39)	missense	probably benign	0.00
IGL02692:Zfp957	APN	14	79,450,825 (GRCm39)	missense	unknown
R0632:Zfp957	UTSW	14	79,450,360 (GRCm39)	missense	probably damaging	1.00
R1018:Zfp957	UTSW	14	79,450,182 (GRCm39)	missense	probably damaging	1.00
R1719:Zfp957	UTSW	14	79,451,436 (GRCm39)	missense	probably damaging	1.00
R2165:Zfp957	UTSW	14	79,451,053 (GRCm39)	missense	probably benign	0.06
R2411:Zfp957	UTSW	14	79,451,782 (GRCm39)	missense	unknown
R2517:Zfp957	UTSW	14	79,451,494 (GRCm39)	missense	probably damaging	0.99
R3195:Zfp957	UTSW	14	79,450,332 (GRCm39)	missense	probably damaging	1.00
R4825:Zfp957	UTSW	14	79,451,796 (GRCm39)	start codon destroyed	probably null
R4881:Zfp957	UTSW	14	79,450,849 (GRCm39)	missense	unknown
R5138:Zfp957	UTSW	14	79,450,362 (GRCm39)	missense	probably damaging	1.00
R5174:Zfp957	UTSW	14	79,450,828 (GRCm39)	missense	unknown
R5531:Zfp957	UTSW	14	79,450,622 (GRCm39)	missense	unknown
R5547:Zfp957	UTSW	14	79,451,406 (GRCm39)	missense	probably benign	0.03
R5677:Zfp957	UTSW	14	79,450,207 (GRCm39)	missense	probably damaging	1.00
R5968:Zfp957	UTSW	14	79,451,496 (GRCm39)	missense	probably damaging	1.00
R6897:Zfp957	UTSW	14	79,451,344 (GRCm39)	missense	probably damaging	0.98
R6994:Zfp957	UTSW	14	79,451,130 (GRCm39)	missense	probably damaging	0.99
R7105:Zfp957	UTSW	14	79,450,402 (GRCm39)	missense	probably benign	0.09
R7214:Zfp957	UTSW	14	79,450,750 (GRCm39)	missense	unknown
R7264:Zfp957	UTSW	14	79,451,080 (GRCm39)	missense	probably damaging	0.98
R8549:Zfp957	UTSW	14	79,451,346 (GRCm39)	missense	probably damaging	1.00
Z1176:Zfp957	UTSW	14	79,451,578 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- GAACCTTGACTTTGCTCTGC -3'
(R):5'- GCTAAAGTACGCTACCCAGC -3'

Sequencing Primer
(F):5'- CATTTCTGTGGACCTCTTAGGAG -3'
(R):5'- CCTTACATCCATGCAGTAAATAAGGG -3'

Posted On

2020-10-20