Mutagenetix > Incidental Mutation

Incidental Mutation 'R8409:Vmn2r85'

652591

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r85

Ensembl Gene

ENSMUSG00000092048

Gene Name

vomeronasal 2, receptor 85

Synonyms

EG623734

MMRRC Submission

067766-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R8409 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130253658-130266615 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 130261257 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 360 (V360A)

Ref Sequence

ENSEMBL: ENSMUSP00000128792 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171811]

AlphaFold

G3UW56

Predicted Effect

probably benign
Transcript: ENSMUST00000171811
AA Change: V360A

PolyPhen 2 Score 0.156 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000128792
Gene: ENSMUSG00000092048
AA Change: V360A

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	9e-26	PFAM
Pfam:NCD3G	508	562	1.1e-18	PFAM
Pfam:7tm_3	595	831	3.7e-54	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam1b	T	G	5: 121,639,540 (GRCm39)	N502H	probably benign	Het
Ahnak	C	T	19: 8,993,037 (GRCm39)	P4774S	probably benign	Het
Bptf	A	T	11: 106,953,495 (GRCm39)	S2082R	probably damaging	Het
Ccdc88a	A	G	11: 29,453,544 (GRCm39)	T1636A	probably benign	Het
Cep83	C	T	10: 94,573,839 (GRCm39)	Q243*	probably null	Het
Dlg5	T	G	14: 24,226,546 (GRCm39)	E452A	probably damaging	Het
Dsg1a	T	A	18: 20,473,208 (GRCm39)	D760E	probably damaging	Het
Elob	G	A	17: 24,043,933 (GRCm39)	R89C	probably benign	Het
En2	T	C	5: 28,371,882 (GRCm39)	S120P	probably benign	Het
Ercc4	G	C	16: 12,948,001 (GRCm39)	R406P	probably benign	Het
Extl2	G	T	3: 115,820,911 (GRCm39)	V253F	probably damaging	Het
Fam217a	T	C	13: 35,100,881 (GRCm39)	E92G	probably benign	Het
Fbxo25	A	T	8: 13,964,999 (GRCm39)	K17*	probably null	Het
Fig4	A	T	10: 41,141,427 (GRCm39)	S277R	probably benign	Het
Gphn	T	C	12: 78,659,784 (GRCm39)	S429P	probably damaging	Het
Grm7	A	G	6: 110,891,297 (GRCm39)	I177V	probably benign	Het
H13	C	T	2: 152,531,813 (GRCm39)	P239L	possibly damaging	Het
Il12rb1	T	A	8: 71,269,187 (GRCm39)	S456T	possibly damaging	Het
Irgq	A	G	7: 24,233,209 (GRCm39)	D350G	probably benign	Het
Isl2	T	C	9: 55,449,784 (GRCm39)	S118P	possibly damaging	Het
Itpka	C	T	2: 119,580,341 (GRCm39)	R329C	probably damaging	Het
Itpripl1	G	T	2: 126,982,686 (GRCm39)	Q479K	probably benign	Het
Kbtbd2	T	C	6: 56,757,341 (GRCm39)	N132D	probably damaging	Het
Klb	T	C	5: 65,536,878 (GRCm39)	V736A	probably damaging	Het
Klhl3	T	C	13: 58,167,242 (GRCm39)	D374G	probably damaging	Het
Mmp14	T	A	14: 54,678,125 (GRCm39)	V582D	probably damaging	Het
Naalad2	A	G	9: 18,242,134 (GRCm39)	V590A	probably damaging	Het
Or2n1d	A	G	17: 38,646,197 (GRCm39)	T50A	probably benign	Het
Or51f1	A	T	7: 102,506,477 (GRCm39)	M4K	probably benign	Het
Or7h8	T	G	9: 20,123,542 (GRCm39)		probably benign	Het
Pianp	T	C	6: 124,976,214 (GRCm39)	S8P	unknown	Het
Ppbp	T	C	5: 90,916,486 (GRCm39)	S9P	probably damaging	Het
Ppp4r3a	A	G	12: 101,008,752 (GRCm39)	I696T	probably benign	Het
Psmd5	A	G	2: 34,760,856 (GRCm39)	S27P	probably damaging	Het
Ralgapa2	T	A	2: 146,086,897 (GRCm39)	R1561S		Het
Rassf8	A	G	6: 145,761,429 (GRCm39)	T252A	probably benign	Het
Rpe65	T	A	3: 159,319,785 (GRCm39)	D218E	probably benign	Het
Sec23ip	G	A	7: 128,365,855 (GRCm39)	V575I	probably damaging	Het
Slc38a3	G	A	9: 107,536,454 (GRCm39)		probably benign	Het
Slco6c1	C	T	1: 97,003,663 (GRCm39)	C495Y	probably damaging	Het
Speer4f2	C	A	5: 17,582,419 (GRCm39)	T214K		Het
Stox1	A	G	10: 62,501,795 (GRCm39)	L255P	probably benign	Het
Sympk	A	G	7: 18,786,363 (GRCm39)	M989V	probably benign	Het
Tia1	T	A	6: 86,402,452 (GRCm39)	Y202N	possibly damaging	Het
Tmem51	TCCCC	TCCC	4: 141,764,996 (GRCm39)		probably null	Het
Usp34	T	C	11: 23,407,811 (GRCm39)	S2593P		Het

Other mutations in Vmn2r85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01298:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01361:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL02185:Vmn2r85	APN	10	130,254,561 (GRCm39)	missense	probably benign	0.13
IGL02505:Vmn2r85	APN	10	130,261,449 (GRCm39)	missense	probably damaging	1.00
IGL02607:Vmn2r85	APN	10	130,262,290 (GRCm39)	missense	possibly damaging	0.89
IGL02755:Vmn2r85	APN	10	130,261,381 (GRCm39)	missense	probably damaging	0.98
IGL03188:Vmn2r85	APN	10	130,254,612 (GRCm39)	missense	probably benign	0.16
IGL03366:Vmn2r85	APN	10	130,262,328 (GRCm39)	missense	probably benign	0.00
IGL03397:Vmn2r85	APN	10	130,261,263 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r85	UTSW	10	130,261,572 (GRCm39)	missense	probably benign	0.00
R0066:Vmn2r85	UTSW	10	130,261,770 (GRCm39)	missense	probably damaging	1.00
R0128:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0130:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0503:Vmn2r85	UTSW	10	130,258,609 (GRCm39)	missense	probably damaging	1.00
R0827:Vmn2r85	UTSW	10	130,265,387 (GRCm39)	missense	possibly damaging	0.89
R1432:Vmn2r85	UTSW	10	130,261,155 (GRCm39)	missense	possibly damaging	0.74
R1521:Vmn2r85	UTSW	10	130,261,788 (GRCm39)	missense	probably damaging	0.99
R2029:Vmn2r85	UTSW	10	130,261,443 (GRCm39)	nonsense	probably null
R2034:Vmn2r85	UTSW	10	130,262,242 (GRCm39)	splice site	probably benign
R2852:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R2853:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R3084:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3085:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3430:Vmn2r85	UTSW	10	130,254,758 (GRCm39)	missense	probably damaging	0.97
R3694:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R3932:Vmn2r85	UTSW	10	130,254,336 (GRCm39)	missense	probably damaging	1.00
R4207:Vmn2r85	UTSW	10	130,254,574 (GRCm39)	missense	probably damaging	1.00
R4628:Vmn2r85	UTSW	10	130,261,235 (GRCm39)	missense	probably benign	0.00
R4814:Vmn2r85	UTSW	10	130,254,567 (GRCm39)	missense	probably benign	0.12
R4948:Vmn2r85	UTSW	10	130,254,990 (GRCm39)	missense	probably damaging	1.00
R4951:Vmn2r85	UTSW	10	130,261,113 (GRCm39)	missense	probably damaging	1.00
R4959:Vmn2r85	UTSW	10	130,257,302 (GRCm39)	missense	probably damaging	1.00
R5336:Vmn2r85	UTSW	10	130,258,574 (GRCm39)	missense	possibly damaging	0.63
R5643:Vmn2r85	UTSW	10	130,262,343 (GRCm39)	missense	probably damaging	1.00
R6061:Vmn2r85	UTSW	10	130,261,531 (GRCm39)	missense	probably benign	0.09
R6115:Vmn2r85	UTSW	10	130,258,672 (GRCm39)	missense	probably damaging	1.00
R6190:Vmn2r85	UTSW	10	130,261,330 (GRCm39)	missense	possibly damaging	0.88
R6518:Vmn2r85	UTSW	10	130,265,281 (GRCm39)	missense	probably benign	0.00
R6533:Vmn2r85	UTSW	10	130,262,529 (GRCm39)	missense	probably benign	0.00
R6610:Vmn2r85	UTSW	10	130,261,838 (GRCm39)	missense	probably damaging	0.97
R6809:Vmn2r85	UTSW	10	130,261,795 (GRCm39)	missense	probably benign
R6962:Vmn2r85	UTSW	10	130,261,452 (GRCm39)	missense	probably damaging	0.99
R7075:Vmn2r85	UTSW	10	130,258,557 (GRCm39)	missense	probably benign	0.06
R7104:Vmn2r85	UTSW	10	130,262,376 (GRCm39)	missense	probably benign
R7424:Vmn2r85	UTSW	10	130,254,849 (GRCm39)	missense	probably damaging	1.00
R7516:Vmn2r85	UTSW	10	130,254,852 (GRCm39)	missense	probably damaging	1.00
R7537:Vmn2r85	UTSW	10	130,258,735 (GRCm39)	missense	probably benign	0.01
R7768:Vmn2r85	UTSW	10	130,254,562 (GRCm39)	missense	probably damaging	1.00
R7810:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R8078:Vmn2r85	UTSW	10	130,265,364 (GRCm39)	nonsense	probably null
R8115:Vmn2r85	UTSW	10	130,261,820 (GRCm39)	missense	probably benign	0.06
R8262:Vmn2r85	UTSW	10	130,254,738 (GRCm39)	missense	probably damaging	0.98
R8395:Vmn2r85	UTSW	10	130,261,797 (GRCm39)	missense	probably damaging	0.99
R8547:Vmn2r85	UTSW	10	130,261,311 (GRCm39)	missense	probably damaging	1.00
R8875:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R9035:Vmn2r85	UTSW	10	130,261,479 (GRCm39)	missense	probably benign
R9040:Vmn2r85	UTSW	10	130,254,311 (GRCm39)	missense	probably damaging	1.00
R9115:Vmn2r85	UTSW	10	130,254,153 (GRCm39)	missense	probably benign	0.00
R9182:Vmn2r85	UTSW	10	130,265,350 (GRCm39)	missense	probably benign	0.00
R9245:Vmn2r85	UTSW	10	130,261,534 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r85	UTSW	10	130,255,033 (GRCm39)	missense	possibly damaging	0.92
R9405:Vmn2r85	UTSW	10	130,261,215 (GRCm39)	missense	probably damaging	0.99
R9502:Vmn2r85	UTSW	10	130,261,387 (GRCm39)	missense	probably damaging	0.99
R9520:Vmn2r85	UTSW	10	130,254,993 (GRCm39)	missense	probably benign
R9653:Vmn2r85	UTSW	10	130,261,694 (GRCm39)	missense	probably damaging	0.99
Z1176:Vmn2r85	UTSW	10	130,261,713 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r85	UTSW	10	130,254,776 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCTGCTGACATTCTGTGTAC -3'
(R):5'- ACCTCACAATGGGATGTTATCAC -3'

Sequencing Primer
(F):5'- GCTGACATTCTGTGTACTTTCC -3'
(R):5'- CACACCACAAAGGTTGGAT -3'

Posted On

2020-10-20