Mutagenetix > Incidental Mutation

Incidental Mutation 'R8411:Vmn2r87'

652687

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r87

Ensembl Gene

ENSMUSG00000091511

Gene Name

vomeronasal 2, receptor 87

Synonyms

EG625131

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R8411 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130471332-130497379 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 130472257 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 704 (I704T)

Ref Sequence

ENSEMBL: ENSMUSP00000129215 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164227]

AlphaFold

E9PZX4

Predicted Effect

probably damaging
Transcript: ENSMUST00000164227
AA Change: I704T

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000129215
Gene: ENSMUSG00000091511
AA Change: I704T

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	422	1.8e-27	PFAM
Pfam:NCD3G	508	562	1.8e-19	PFAM
Pfam:7tm_3	595	829	8.8e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900026A02Rik	A	G	5: 113,137,722	S89P	probably benign	Het
Aatf	A	T	11: 84,470,676	M367K	probably benign	Het
Adam18	A	T	8: 24,652,127	I211N	probably damaging	Het
Angpt1	T	C	15: 42,427,034	Y478C	probably damaging	Het
Apba2	A	T	7: 64,736,926	I434F	probably damaging	Het
Arfgef1	T	A	1: 10,216,534	K50N	probably benign	Het
Arfgef2	A	T	2: 166,873,983	Q1397H	probably benign	Het
Ascc2	G	A	11: 4,647,208	R129Q	probably damaging	Het
Atxn1	A	G	13: 45,566,556	V621A	probably benign	Het
Catsperz	A	T	19: 6,922,562	L192M	probably benign	Het
Cd5	G	A	19: 10,720,221	P465S	probably damaging	Het
Cfap57	C	T	4: 118,614,931	V84I	probably benign	Het
Cfap61	A	G	2: 145,947,183	E94G	probably benign	Het
Chd1	A	G	17: 15,762,449	H1392R	probably damaging	Het
Csnk1a1	A	G	18: 61,555,817	I23V	probably benign	Het
Ctnnd2	C	T	15: 30,647,033	R292C	probably benign	Het
Dcaf6	T	A	1: 165,388,675	H453L	probably benign	Het
Dnah3	C	A	7: 120,011,030	D1728Y	probably damaging	Het
Dtx3	T	C	10: 127,192,824	K179E	possibly damaging	Het
Fam3b	T	A	16: 97,481,853	Y74F	probably benign	Het
Gm5464	T	C	14: 66,869,106	L64P	unknown	Het
Gm5624	T	G	14: 44,561,890	N70T		Het
Kcnh7	T	A	2: 62,764,608	H706L	probably damaging	Het
Kdm2b	C	T	5: 122,880,176	R1067H	probably damaging	Het
Klhl28	A	T	12: 64,950,090	H492Q	probably damaging	Het
Loxl3	T	A	6: 83,050,624	C716S	probably damaging	Het
Ltbp2	A	G	12: 84,786,413	Y1474H	probably damaging	Het
Mcm3	C	T	1: 20,816,756	V142I	probably benign	Het
Mmp24	G	T	2: 155,814,015	V458L	probably benign	Het
Mpl	C	A	4: 118,446,109	S502I		Het
Nfatc1	A	T	18: 80,667,042	V503D	probably damaging	Het
Nim1k	A	G	13: 119,714,271	I133T	possibly damaging	Het
Nr1d2	T	C	14: 18,215,031	Y327C	probably damaging	Het
Olfr1214	T	C	2: 88,988,065	T46A	probably benign	Het
Oma1	C	T	4: 103,328,916	R360*	probably null	Het
Oog2	T	A	4: 144,194,173	W59R	probably damaging	Het
Pcdh10	A	G	3: 45,379,539	E96G	probably damaging	Het
Pcna	T	A	2: 132,251,930	T98S	probably benign	Het
Pcnx3	C	T	19: 5,679,590	C899Y	possibly damaging	Het
Phf11d	T	C	14: 59,356,434	N97S	probably benign	Het
Plekhg4	G	A	8: 105,377,329	W431*	probably null	Het
Plk4	A	G	3: 40,813,466	T815A	probably benign	Het
Pnma2	C	T	14: 66,916,313	T62I	possibly damaging	Het
Ppp1r9a	C	T	6: 5,057,568	R548W	probably damaging	Het
Ptx3	A	G	3: 66,224,780	S241G	probably benign	Het
Repin1	G	T	6: 48,597,345	E403*	probably null	Het
Rplp0	A	T	5: 115,560,764	K26N	probably damaging	Het
Sec62	A	C	3: 30,818,782	E338A	unknown	Het
Sema6a	T	C	18: 47,248,955	M842V	probably benign	Het
Serpina3j	T	C	12: 104,314,784	V72A	probably benign	Het
Siglecf	T	C	7: 43,351,944	F112S	probably damaging	Het
Slc29a4	A	G	5: 142,720,125	N455D	probably damaging	Het
Slc6a11	T	A	6: 114,131,437	F54Y	probably benign	Het
Spag6	G	A	2: 18,710,583	V80M	probably damaging	Het
Spic	T	C	10: 88,678,636	E34G	possibly damaging	Het
Tecpr2	A	G	12: 110,931,720	K469E	possibly damaging	Het
Them7	T	A	2: 105,297,845	L57Q	probably benign	Het
Tlr3	G	A	8: 45,396,941	A897V	probably damaging	Het
Tnp2	A	G	16: 10,788,508	C32R	possibly damaging	Het
Trpm6	A	T	19: 18,853,968	Q1399L	probably benign	Het
Ttn	C	A	2: 76,846,671	E11074*	probably null	Het
Ubash3b	G	A	9: 41,043,485	A243V	probably benign	Het
Vash1	G	A	12: 86,680,178	R64Q	possibly damaging	Het
Vmn1r167	T	A	7: 23,505,556	M12L	possibly damaging	Het
Vmn2r111	T	A	17: 22,548,581	H645L	probably benign	Het
Vmn2r75	A	T	7: 86,148,514	I697K	probably damaging	Het

Other mutations in Vmn2r87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01090:Vmn2r87	APN	10	130497378	start codon destroyed	probably null	1.00
IGL01295:Vmn2r87	APN	10	130472009	missense	probably damaging	1.00
IGL01411:Vmn2r87	APN	10	130472560	missense	probably benign	0.03
IGL01680:Vmn2r87	APN	10	130479717	nonsense	probably null
IGL01822:Vmn2r87	APN	10	130472122	missense	probably damaging	1.00
IGL01835:Vmn2r87	APN	10	130479109	missense	probably damaging	1.00
IGL01965:Vmn2r87	APN	10	130479055	missense	possibly damaging	0.49
IGL02562:Vmn2r87	APN	10	130478644	missense	probably damaging	1.00
IGL02665:Vmn2r87	APN	10	130497180	missense	probably benign	0.16
IGL03202:Vmn2r87	APN	10	130497222	missense	probably benign
FR4304:Vmn2r87	UTSW	10	130478714	missense	probably benign	0.01
FR4340:Vmn2r87	UTSW	10	130478714	missense	probably benign	0.01
FR4342:Vmn2r87	UTSW	10	130478714	missense	probably benign	0.01
FR4589:Vmn2r87	UTSW	10	130478714	missense	probably benign	0.01
LCD18:Vmn2r87	UTSW	10	130478714	missense	probably benign	0.01
R0344:Vmn2r87	UTSW	10	130479937	missense	probably damaging	1.00
R0374:Vmn2r87	UTSW	10	130471979	missense	probably damaging	1.00
R0384:Vmn2r87	UTSW	10	130471843	missense	probably benign
R1144:Vmn2r87	UTSW	10	130476229	splice site	probably benign
R1172:Vmn2r87	UTSW	10	130477584	missense	probably benign	0.03
R1860:Vmn2r87	UTSW	10	130479886	missense	probably benign	0.00
R1866:Vmn2r87	UTSW	10	130472572	missense	possibly damaging	0.88
R1897:Vmn2r87	UTSW	10	130471960	missense	probably damaging	1.00
R2360:Vmn2r87	UTSW	10	130479762	missense	probably damaging	0.99
R2909:Vmn2r87	UTSW	10	130478996	missense	probably damaging	0.99
R3874:Vmn2r87	UTSW	10	130479987	missense	possibly damaging	0.62
R4113:Vmn2r87	UTSW	10	130479822	missense	probably benign
R4190:Vmn2r87	UTSW	10	130472687	missense	probably damaging	1.00
R4197:Vmn2r87	UTSW	10	130479910	missense	possibly damaging	0.55
R4201:Vmn2r87	UTSW	10	130472579	missense	probably benign	0.03
R4202:Vmn2r87	UTSW	10	130472579	missense	probably benign	0.03
R4368:Vmn2r87	UTSW	10	130479807	missense	probably benign	0.44
R4485:Vmn2r87	UTSW	10	130479809	nonsense	probably null
R4537:Vmn2r87	UTSW	10	130472185	missense	probably benign	0.12
R4590:Vmn2r87	UTSW	10	130479145	missense	possibly damaging	0.69
R4752:Vmn2r87	UTSW	10	130478467	nonsense	probably null
R4873:Vmn2r87	UTSW	10	130472498	missense	probably damaging	1.00
R4875:Vmn2r87	UTSW	10	130472498	missense	probably damaging	1.00
R4923:Vmn2r87	UTSW	10	130478566	missense	probably damaging	0.99
R4970:Vmn2r87	UTSW	10	130478553	missense	probably damaging	1.00
R5049:Vmn2r87	UTSW	10	130472429	missense	probably damaging	0.96
R5112:Vmn2r87	UTSW	10	130478553	missense	probably damaging	1.00
R5187:Vmn2r87	UTSW	10	130497339	missense	probably null	0.99
R5618:Vmn2r87	UTSW	10	130479948	missense	probably damaging	1.00
R6057:Vmn2r87	UTSW	10	130472357	missense	probably benign	0.02
R6220:Vmn2r87	UTSW	10	130479938	missense	probably benign	0.01
R6287:Vmn2r87	UTSW	10	130478422	critical splice donor site	probably null
R6383:Vmn2r87	UTSW	10	130479000	missense	probably damaging	1.00
R6576:Vmn2r87	UTSW	10	130478785	missense	probably benign	0.05
R6742:Vmn2r87	UTSW	10	130472527	missense	probably damaging	1.00
R7086:Vmn2r87	UTSW	10	130497309	missense	probably benign	0.00
R7162:Vmn2r87	UTSW	10	130477547	missense	probably benign	0.08
R7419:Vmn2r87	UTSW	10	130472123	missense	probably damaging	1.00
R7425:Vmn2r87	UTSW	10	130478892	missense	probably damaging	1.00
R7443:Vmn2r87	UTSW	10	130472719	missense	probably damaging	1.00
R7571:Vmn2r87	UTSW	10	130479071	missense	probably damaging	0.99
R7663:Vmn2r87	UTSW	10	130472185	missense	probably damaging	0.97
R7716:Vmn2r87	UTSW	10	130472149	missense	probably benign	0.09
R7793:Vmn2r87	UTSW	10	130477544	missense	probably benign	0.05
R7806:Vmn2r87	UTSW	10	130479810	missense	probably benign
R7841:Vmn2r87	UTSW	10	130497226	missense	probably benign	0.31
R8326:Vmn2r87	UTSW	10	130472311	missense	possibly damaging	0.67
R8445:Vmn2r87	UTSW	10	130477466	missense	probably damaging	1.00
R8540:Vmn2r87	UTSW	10	130478893	missense	possibly damaging	0.74
R8892:Vmn2r87	UTSW	10	130472236	missense	probably damaging	0.99
R9093:Vmn2r87	UTSW	10	130472296	missense	probably benign	0.08
R9623:Vmn2r87	UTSW	10	130479925	missense	probably damaging	1.00
R9667:Vmn2r87	UTSW	10	130478907	missense	probably damaging	1.00
R9797:Vmn2r87	UTSW	10	130472269	missense	probably damaging	0.99
R9797:Vmn2r87	UTSW	10	130476195	missense	probably benign	0.44
Z1088:Vmn2r87	UTSW	10	130472314	missense	probably damaging	0.98
Z1176:Vmn2r87	UTSW	10	130471844	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- ACACCAGCATGCTGAAAGTTAG -3'
(R):5'- TTGAACATCCCAACCAGGTC -3'

Sequencing Primer
(F):5'- GCTTCATTGAATCTGTCAGGAAG -3'
(R):5'- AGGTCACCTGCATCCTGC -3'

Posted On

2020-10-20