Mutagenetix > Incidental Mutation

Incidental Mutation 'R8415:Or7e170'

652895

Institutional Source

Beutler Lab

Gene Symbol

Or7e170

Ensembl Gene

ENSMUSG00000063842

Gene Name

olfactory receptor family 7 subfamily E member 170

Synonyms

Olfr862, MOR146-1, GA_x6K02T2PVTD-13624132-13623212

MMRRC Submission

067769-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R8415 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19794679-19795969 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19795409 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 64 (Y64F)

Ref Sequence

ENSEMBL: ENSMUSP00000078603 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073765] [ENSMUST00000079660]

AlphaFold

Q8VFJ1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000073765
AA Change: Y64F

PolyPhen 2 Score 0.955 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000073437
Gene: ENSMUSG00000063842
AA Change: Y64F

Domain	Start	End	E-Value	Type
low complexity region	6	17	N/A	INTRINSIC
Pfam:7tm_4	31	306	3.1e-52	PFAM
Pfam:7TM_GPCR_Srsx	35	282	1.3e-7	PFAM
Pfam:7tm_1	41	290	5.7e-24	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000079660
AA Change: Y64F

PolyPhen 2 Score 0.955 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000078603
Gene: ENSMUSG00000063842
AA Change: Y64F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.3e-51	PFAM
Pfam:7TM_GPCR_Srsx	35	303	8.3e-8	PFAM
Pfam:7tm_1	41	290	4.5e-22	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd24	A	G	10: 81,475,947 (GRCm39)	H317R	unknown	Het
Aox1	A	T	1: 58,080,638 (GRCm39)	Y29F	probably damaging	Het
Bnip2	T	C	9: 69,910,967 (GRCm39)	F304S	possibly damaging	Het
Bsn	G	A	9: 107,988,651 (GRCm39)	A2367V	probably benign	Het
Ccdc18	T	A	5: 108,363,899 (GRCm39)	V1198E	probably damaging	Het
Cnot3	T	C	7: 3,661,687 (GRCm39)	F660L	probably benign	Het
Cntnap3	A	G	13: 64,886,479 (GRCm39)	I1249T	probably benign	Het
Ctnna3	A	G	10: 63,339,909 (GRCm39)	I32V	probably benign	Het
Dgkz	A	T	2: 91,770,649 (GRCm39)	S485T	possibly damaging	Het
Dnaaf6rt	A	G	1: 31,262,564 (GRCm39)	N182S	probably benign	Het
Dpagt1	C	A	9: 44,238,482 (GRCm39)	N49K	possibly damaging	Het
Dsg3	A	T	18: 20,656,765 (GRCm39)	S212C	probably damaging	Het
Epb41l1	C	T	2: 156,368,873 (GRCm39)	A714V	probably benign	Het
Eppk1	A	G	15: 75,995,831 (GRCm39)	V350A	probably benign	Het
Frem1	T	G	4: 82,918,499 (GRCm39)	D481A	probably damaging	Het
Git2	T	C	5: 114,871,989 (GRCm39)	K532E	possibly damaging	Het
Glyr1	T	C	16: 4,854,329 (GRCm39)	H102R	probably benign	Het
Hdgfl2	C	T	17: 56,400,712 (GRCm39)	P160L	probably benign	Het
Hivep1	T	C	13: 42,308,905 (GRCm39)	S382P	probably benign	Het
Hmcn2	C	A	2: 31,281,088 (GRCm39)	L1867I	probably benign	Het
Hydin	G	A	8: 111,178,626 (GRCm39)	G1074R	probably damaging	Het
Ift140	A	G	17: 25,311,889 (GRCm39)	I1271V	probably damaging	Het
Krt28	T	A	11: 99,265,626 (GRCm39)	I14L	probably benign	Het
Lrtm2	A	T	6: 119,294,458 (GRCm39)	D224E	probably damaging	Het
Map1s	G	A	8: 71,365,910 (GRCm39)	V272M	probably damaging	Het
Mast3	A	G	8: 71,233,866 (GRCm39)	Y960H	probably damaging	Het
Mettl25	G	T	10: 105,662,002 (GRCm39)	Q323K	probably benign	Het
Mlh3	A	T	12: 85,315,854 (GRCm39)	S111T	probably benign	Het
Muc17	A	G	5: 137,172,798 (GRCm39)	L85P		Het
Myh6	C	G	14: 55,181,835 (GRCm39)	R1822P	probably damaging	Het
Myof	C	T	19: 37,983,872 (GRCm39)	G144E	probably benign	Het
Nat8f2	A	G	6: 85,845,024 (GRCm39)	S113P	probably damaging	Het
Nlrp9a	A	T	7: 26,256,925 (GRCm39)	Y181F	probably benign	Het
Nup85	A	G	11: 115,457,468 (GRCm39)	I57V	probably benign	Het
Or4c99	T	A	2: 88,329,710 (GRCm39)	C94S	probably damaging	Het
Or5p79	C	T	7: 108,221,163 (GRCm39)	T48I	probably damaging	Het
Pcdhb2	A	G	18: 37,429,110 (GRCm39)	N361S	probably damaging	Het
Peg10	T	TCCA	6: 4,756,451 (GRCm39)		probably benign	Het
Phf20	C	T	2: 156,129,913 (GRCm39)	T495M	probably benign	Het
Pitpnm1	T	C	19: 4,155,454 (GRCm39)	M368T	probably benign	Het
Pter	A	G	2: 13,005,700 (GRCm39)	D291G	probably damaging	Het
Rasgrp2	T	G	19: 6,454,781 (GRCm39)	V207G	probably damaging	Het
Rnd2	A	T	11: 101,362,011 (GRCm39)	Q188L	probably benign	Het
Sh3d19	T	A	3: 85,992,363 (GRCm39)	D130E	probably benign	Het
Slc7a2	A	T	8: 41,369,396 (GRCm39)	Y617F	probably damaging	Het
Snx29	T	C	16: 11,265,291 (GRCm39)	L165P	probably damaging	Het
St7	T	C	6: 17,848,080 (GRCm39)		probably null	Het
Tas2r115	A	G	6: 132,714,798 (GRCm39)	L51P	probably damaging	Het
Tead1	C	A	7: 112,456,135 (GRCm39)	P164Q	probably benign	Het
Thop1	A	G	10: 80,914,385 (GRCm39)	Y224C	probably damaging	Het
Twf1	T	C	15: 94,477,702 (GRCm39)	*351W	probably null	Het
Uck1	C	A	2: 32,150,153 (GRCm39)		probably benign	Het
Utp20	A	G	10: 88,662,466 (GRCm39)		probably null	Het
Vmn2r118	G	T	17: 55,915,057 (GRCm39)	L530I	probably benign	Het
Vmn2r30	A	T	7: 7,315,359 (GRCm39)	C825S	probably damaging	Het
Vps13d	A	T	4: 144,818,549 (GRCm39)	M3106K		Het
Wfdc15a	A	T	2: 164,041,671 (GRCm39)	C51S	probably damaging	Het
Zc3hc1	T	C	6: 30,375,951 (GRCm39)	D203G	probably damaging	Het
Zfhx2	G	T	14: 55,308,079 (GRCm39)	D932E	probably benign	Het
Zfp267	G	A	3: 36,219,182 (GRCm39)	G402S	probably damaging	Het

Other mutations in Or7e170

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01114:Or7e170	APN	9	19,794,844 (GRCm39)	nonsense	probably null
IGL01636:Or7e170	APN	9	19,795,484 (GRCm39)	missense	probably benign	0.14
IGL01765:Or7e170	APN	9	19,795,247 (GRCm39)	missense	possibly damaging	0.71
IGL01967:Or7e170	APN	9	19,794,885 (GRCm39)	missense	probably damaging	0.97
IGL03145:Or7e170	APN	9	19,794,735 (GRCm39)	missense	possibly damaging	0.93
IGL03222:Or7e170	APN	9	19,795,495 (GRCm39)	nonsense	probably null
R0117:Or7e170	UTSW	9	19,795,595 (GRCm39)	missense	probably damaging	0.96
R0662:Or7e170	UTSW	9	19,795,248 (GRCm39)	missense	probably benign	0.32
R2399:Or7e170	UTSW	9	19,795,220 (GRCm39)	missense	probably damaging	0.98
R4224:Or7e170	UTSW	9	19,794,896 (GRCm39)	missense	probably benign	0.44
R4572:Or7e170	UTSW	9	19,795,275 (GRCm39)	missense	probably benign
R5607:Or7e170	UTSW	9	19,795,272 (GRCm39)	missense	probably benign	0.16
R5741:Or7e170	UTSW	9	19,794,857 (GRCm39)	missense	possibly damaging	0.92
R5759:Or7e170	UTSW	9	19,795,484 (GRCm39)	missense	probably benign	0.14
R6237:Or7e170	UTSW	9	19,795,365 (GRCm39)	missense	probably damaging	1.00
R6434:Or7e170	UTSW	9	19,795,141 (GRCm39)	nonsense	probably null
R7075:Or7e170	UTSW	9	19,795,359 (GRCm39)	missense	probably benign	0.16
R7534:Or7e170	UTSW	9	19,795,472 (GRCm39)	missense	probably benign	0.16
R7735:Or7e170	UTSW	9	19,795,410 (GRCm39)	missense	probably damaging	0.99
R9246:Or7e170	UTSW	9	19,795,686 (GRCm39)	start gained	probably benign
R9438:Or7e170	UTSW	9	19,795,083 (GRCm39)	missense	probably benign
R9795:Or7e170	UTSW	9	19,795,347 (GRCm39)	missense	probably benign
Z1177:Or7e170	UTSW	9	19,794,963 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCGGGGTTCAGAATGACTTG -3'
(R):5'- TTTAACAGGTGCCTTGGAATTCC -3'

Sequencing Primer
(F):5'- TGGCTACCCATCGGTCATAG -3'
(R):5'- CTGTTACTTGGTCTCTCAGAGGAC -3'

Posted On

2020-10-20