Incidental Mutation 'R8416:Asap1'
ID 652965
Institutional Source Beutler Lab
Gene Symbol Asap1
Ensembl Gene ENSMUSG00000022377
Gene Name ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms Ddef1
MMRRC Submission 067770-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.114) question?
Stock # R8416 (G1)
Quality Score 225.009
Status Not validated
Chromosome 15
Chromosomal Location 63958706-64254768 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 64002072 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Methionine to Lysine at position 413 (M413K)
Ref Sequence ENSEMBL: ENSMUSP00000135643 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000023008] [ENSMUST00000110114] [ENSMUST00000110115] [ENSMUST00000175793] [ENSMUST00000175799] [ENSMUST00000176014] [ENSMUST00000177371] [ENSMUST00000176384] [ENSMUST00000177035] [ENSMUST00000177083] [ENSMUST00000177374]
AlphaFold Q9QWY8
Predicted Effect possibly damaging
Transcript: ENSMUST00000023008
AA Change: M436K

PolyPhen 2 Score 0.835 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000023008
Gene: ENSMUSG00000022377
AA Change: M436K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
low complexity region 122 136 N/A INTRINSIC
low complexity region 147 164 N/A INTRINSIC
PH 340 433 4.12e-15 SMART
ArfGap 454 577 2.18e-34 SMART
ANK 615 647 1.17e-1 SMART
ANK 651 680 3.46e-4 SMART
low complexity region 727 738 N/A INTRINSIC
low complexity region 792 803 N/A INTRINSIC
low complexity region 814 847 N/A INTRINSIC
low complexity region 856 865 N/A INTRINSIC
low complexity region 892 903 N/A INTRINSIC
low complexity region 971 984 N/A INTRINSIC
low complexity region 1065 1077 N/A INTRINSIC
SH3 1088 1146 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000110114
AA Change: M436K

PolyPhen 2 Score 0.835 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000105741
Gene: ENSMUSG00000022377
AA Change: M436K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
low complexity region 122 136 N/A INTRINSIC
low complexity region 147 164 N/A INTRINSIC
PH 340 433 4.12e-15 SMART
ArfGap 454 577 2.18e-34 SMART
ANK 615 647 1.17e-1 SMART
ANK 651 680 3.46e-4 SMART
low complexity region 727 738 N/A INTRINSIC
low complexity region 792 803 N/A INTRINSIC
low complexity region 835 846 N/A INTRINSIC
low complexity region 914 927 N/A INTRINSIC
low complexity region 1008 1020 N/A INTRINSIC
SH3 1031 1089 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000110115
AA Change: M421K

PolyPhen 2 Score 0.877 (Sensitivity: 0.83; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000105742
Gene: ENSMUSG00000022377
AA Change: M421K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
low complexity region 122 136 N/A INTRINSIC
low complexity region 147 164 N/A INTRINSIC
PH 325 418 4.12e-15 SMART
ArfGap 439 562 2.18e-34 SMART
ANK 600 632 1.17e-1 SMART
ANK 636 665 3.46e-4 SMART
low complexity region 712 723 N/A INTRINSIC
low complexity region 777 788 N/A INTRINSIC
low complexity region 799 832 N/A INTRINSIC
low complexity region 841 850 N/A INTRINSIC
low complexity region 877 888 N/A INTRINSIC
low complexity region 956 969 N/A INTRINSIC
low complexity region 1050 1062 N/A INTRINSIC
SH3 1073 1131 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000175793
AA Change: M424K

PolyPhen 2 Score 0.940 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000135718
Gene: ENSMUSG00000022377
AA Change: M424K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
low complexity region 122 136 N/A INTRINSIC
low complexity region 147 164 N/A INTRINSIC
PH 328 421 4.12e-15 SMART
ArfGap 442 565 2.18e-34 SMART
ANK 603 635 1.17e-1 SMART
ANK 639 668 3.46e-4 SMART
low complexity region 715 726 N/A INTRINSIC
low complexity region 780 791 N/A INTRINSIC
low complexity region 802 835 N/A INTRINSIC
low complexity region 844 853 N/A INTRINSIC
low complexity region 880 891 N/A INTRINSIC
low complexity region 959 972 N/A INTRINSIC
low complexity region 1053 1065 N/A INTRINSIC
SH3 1076 1134 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000175799
AA Change: M433K

PolyPhen 2 Score 0.835 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000135359
Gene: ENSMUSG00000022377
AA Change: M433K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
low complexity region 122 136 N/A INTRINSIC
low complexity region 147 164 N/A INTRINSIC
PH 337 430 4.12e-15 SMART
ArfGap 451 574 2.18e-34 SMART
ANK 612 644 1.17e-1 SMART
ANK 648 677 3.46e-4 SMART
low complexity region 724 735 N/A INTRINSIC
low complexity region 789 800 N/A INTRINSIC
low complexity region 832 843 N/A INTRINSIC
low complexity region 911 924 N/A INTRINSIC
low complexity region 1005 1017 N/A INTRINSIC
SH3 1028 1086 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000176014
AA Change: M433K

PolyPhen 2 Score 0.835 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000135172
Gene: ENSMUSG00000022377
AA Change: M433K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
low complexity region 122 136 N/A INTRINSIC
low complexity region 147 164 N/A INTRINSIC
PH 337 430 4.12e-15 SMART
ArfGap 451 574 2.18e-34 SMART
ANK 612 644 1.17e-1 SMART
ANK 648 677 3.46e-4 SMART
low complexity region 724 735 N/A INTRINSIC
low complexity region 789 800 N/A INTRINSIC
low complexity region 811 844 N/A INTRINSIC
low complexity region 853 862 N/A INTRINSIC
low complexity region 889 900 N/A INTRINSIC
low complexity region 968 981 N/A INTRINSIC
low complexity region 1062 1074 N/A INTRINSIC
SH3 1085 1143 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000177371
AA Change: M413K

PolyPhen 2 Score 0.835 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000135643
Gene: ENSMUSG00000022377
AA Change: M413K

DomainStartEndE-ValueType
low complexity region 102 116 N/A INTRINSIC
low complexity region 127 144 N/A INTRINSIC
PH 317 410 4.12e-15 SMART
ArfGap 431 554 2.18e-34 SMART
ANK 592 624 1.17e-1 SMART
ANK 628 657 3.46e-4 SMART
low complexity region 704 715 N/A INTRINSIC
low complexity region 769 780 N/A INTRINSIC
low complexity region 791 824 N/A INTRINSIC
low complexity region 833 842 N/A INTRINSIC
low complexity region 869 880 N/A INTRINSIC
low complexity region 948 961 N/A INTRINSIC
low complexity region 1042 1054 N/A INTRINSIC
SH3 1065 1123 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000176384
AA Change: M436K

PolyPhen 2 Score 0.940 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000135190
Gene: ENSMUSG00000022377
AA Change: M436K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
low complexity region 122 136 N/A INTRINSIC
low complexity region 147 164 N/A INTRINSIC
low complexity region 310 328 N/A INTRINSIC
PH 340 433 4.12e-15 SMART
ArfGap 454 577 2.18e-34 SMART
ANK 615 647 1.17e-1 SMART
ANK 651 680 3.46e-4 SMART
low complexity region 727 738 N/A INTRINSIC
low complexity region 792 803 N/A INTRINSIC
low complexity region 835 846 N/A INTRINSIC
low complexity region 914 927 N/A INTRINSIC
low complexity region 1008 1020 N/A INTRINSIC
SH3 1031 1089 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000177035
AA Change: M421K

PolyPhen 2 Score 0.805 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000135346
Gene: ENSMUSG00000022377
AA Change: M421K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
low complexity region 122 136 N/A INTRINSIC
low complexity region 147 164 N/A INTRINSIC
PH 325 418 4.12e-15 SMART
ArfGap 439 562 2.18e-34 SMART
ANK 600 632 1.17e-1 SMART
ANK 636 665 3.46e-4 SMART
low complexity region 712 723 N/A INTRINSIC
low complexity region 777 788 N/A INTRINSIC
low complexity region 820 831 N/A INTRINSIC
low complexity region 899 912 N/A INTRINSIC
low complexity region 993 1005 N/A INTRINSIC
SH3 1016 1074 3.29e-17 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000177083
AA Change: M401K

PolyPhen 2 Score 0.805 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000134877
Gene: ENSMUSG00000022377
AA Change: M401K

DomainStartEndE-ValueType
low complexity region 102 116 N/A INTRINSIC
low complexity region 127 144 N/A INTRINSIC
PH 305 398 4.12e-15 SMART
ArfGap 419 542 2.18e-34 SMART
ANK 580 612 1.17e-1 SMART
ANK 616 645 3.46e-4 SMART
low complexity region 692 703 N/A INTRINSIC
low complexity region 757 768 N/A INTRINSIC
low complexity region 779 812 N/A INTRINSIC
low complexity region 821 830 N/A INTRINSIC
low complexity region 857 868 N/A INTRINSIC
low complexity region 936 949 N/A INTRINSIC
low complexity region 1030 1042 N/A INTRINSIC
SH3 1053 1111 3.29e-17 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000177374
AA Change: M436K

PolyPhen 2 Score 0.037 (Sensitivity: 0.94; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000134825
Gene: ENSMUSG00000022377
AA Change: M436K

DomainStartEndE-ValueType
low complexity region 2 16 N/A INTRINSIC
Pfam:BAR 18 267 1.8e-11 PFAM
Pfam:BAR_3 52 286 1.2e-29 PFAM
low complexity region 310 328 N/A INTRINSIC
PH 340 433 4.12e-15 SMART
ArfGap 454 577 2.18e-34 SMART
ANK 615 647 1.17e-1 SMART
ANK 651 680 3.46e-4 SMART
low complexity region 727 738 N/A INTRINSIC
low complexity region 792 803 N/A INTRINSIC
low complexity region 814 847 N/A INTRINSIC
low complexity region 856 865 N/A INTRINSIC
low complexity region 892 903 N/A INTRINSIC
low complexity region 971 984 N/A INTRINSIC
low complexity region 1065 1077 N/A INTRINSIC
SH3 1088 1146 3.29e-17 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Allele List at MGI

All alleles(13) : Gene trapped(13)

Other mutations in this stock
Total: 53 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcf3 A G 16: 20,369,023 (GRCm39) N173S probably benign Het
Agps A G 2: 75,684,547 (GRCm39) I172V probably benign Het
Amz1 G A 5: 140,737,731 (GRCm39) W330* probably null Het
Bhmt1b T C 18: 87,774,687 (GRCm39) V70A probably damaging Het
Ccdc83 A T 7: 89,885,513 (GRCm39) W291R unknown Het
Cdh19 A T 1: 110,853,610 (GRCm39) M277K probably benign Het
Clca3a1 C A 3: 144,460,914 (GRCm39) probably null Het
Cnpy1 T C 5: 28,408,423 (GRCm39) T138A probably benign Het
Csnka2ip T C 16: 64,300,295 (GRCm39) D23G Het
Cxcr5 A T 9: 44,425,583 (GRCm39) F25I probably benign Het
Defb33 T A 8: 21,387,651 (GRCm39) *63R probably null Het
Dgkz A T 2: 91,770,649 (GRCm39) S485T possibly damaging Het
Elac1 A G 18: 73,871,927 (GRCm39) I356T probably damaging Het
Fam135a C T 1: 24,067,675 (GRCm39) D1065N probably benign Het
Fbxo10 T A 4: 45,058,942 (GRCm39) D265V possibly damaging Het
Flrt2 A G 12: 95,746,331 (GRCm39) E223G probably benign Het
Gjc2 A T 11: 59,068,334 (GRCm39) D49E probably damaging Het
Hmcn2 C A 2: 31,281,088 (GRCm39) L1867I probably benign Het
Irf2 A T 8: 47,271,887 (GRCm39) Y158F probably benign Het
Jmy T C 13: 93,634,949 (GRCm39) D289G probably damaging Het
Krt1 AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC 15: 101,758,813 (GRCm39) probably benign Het
Limch1 T G 5: 67,156,649 (GRCm39) I372S probably benign Het
Lsm11 A T 11: 45,824,730 (GRCm39) W266R probably benign Het
Mboat2 G A 12: 24,984,609 (GRCm39) E147K probably damaging Het
Mctp2 T C 7: 71,852,210 (GRCm39) I439V probably benign Het
Npc2 A G 12: 84,812,131 (GRCm39) V37A probably damaging Het
Or2t29 A T 11: 58,433,778 (GRCm39) S188T possibly damaging Het
Or5b119 A T 19: 13,456,764 (GRCm39) M266K possibly damaging Het
Parp4 T C 14: 56,825,271 (GRCm39) probably null Het
Pcdhga2 A T 18: 37,803,178 (GRCm39) N341Y probably damaging Het
Pde1b G A 15: 103,423,745 (GRCm39) probably benign Het
Pde1c C T 6: 56,128,276 (GRCm39) probably null Het
Plce1 G A 19: 38,761,441 (GRCm39) V2098I possibly damaging Het
Pou2f2 G T 7: 24,815,551 (GRCm39) S72* probably null Het
Ptpru C T 4: 131,535,783 (GRCm39) R398H probably damaging Het
Rps15 A G 10: 80,128,624 (GRCm39) K7E probably benign Het
Rtn4rl2 C T 2: 84,702,951 (GRCm39) G207D probably damaging Het
Scn2a A C 2: 65,511,345 (GRCm39) L130F probably benign Het
Sh3tc1 G A 5: 35,868,256 (GRCm39) R403W probably damaging Het
Slc7a9 A G 7: 35,152,858 (GRCm39) K92R probably benign Het
Slit3 A T 11: 35,399,062 (GRCm39) E142D probably benign Het
Srpk1 A G 17: 28,813,229 (GRCm39) I505T probably damaging Het
Sult1c2 A T 17: 54,269,580 (GRCm39) I216K probably benign Het
Susd6 T A 12: 80,897,997 (GRCm39) M44K probably benign Het
Thoc5 T A 11: 4,876,068 (GRCm39) V607E probably benign Het
Tiparp A G 3: 65,438,767 (GRCm39) R28G probably benign Het
Tln1 C T 4: 43,540,116 (GRCm39) V1498I probably benign Het
Tmprss11c T G 5: 86,387,276 (GRCm39) Q214P probably damaging Het
Uck1 C A 2: 32,150,153 (GRCm39) probably benign Het
Wdr11 G A 7: 129,232,403 (GRCm39) S937N possibly damaging Het
Zdhhc23 A T 16: 43,791,927 (GRCm39) I282K probably damaging Het
Zfp488 A G 14: 33,693,052 (GRCm39) M37T probably benign Het
Zmpste24 T C 4: 120,940,556 (GRCm39) S142G probably benign Het
Other mutations in Asap1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00428:Asap1 APN 15 63,991,803 (GRCm39) splice site probably benign
IGL00473:Asap1 APN 15 64,045,064 (GRCm39) splice site probably benign
IGL00519:Asap1 APN 15 63,982,791 (GRCm39) missense probably damaging 1.00
IGL01304:Asap1 APN 15 64,184,298 (GRCm39) missense probably damaging 1.00
IGL01510:Asap1 APN 15 64,030,777 (GRCm39) missense probably damaging 1.00
IGL02208:Asap1 APN 15 63,993,882 (GRCm39) missense probably damaging 1.00
IGL02338:Asap1 APN 15 63,995,519 (GRCm39) critical splice donor site probably null
IGL02429:Asap1 APN 15 64,039,589 (GRCm39) missense probably damaging 1.00
IGL02565:Asap1 APN 15 64,001,014 (GRCm39) splice site probably benign
IGL02644:Asap1 APN 15 63,982,911 (GRCm39) missense probably damaging 1.00
IGL02684:Asap1 APN 15 63,966,018 (GRCm39) missense probably benign
IGL02707:Asap1 APN 15 64,001,123 (GRCm39) missense probably damaging 1.00
IGL03052:Asap1 APN 15 64,025,683 (GRCm39) splice site probably benign
IGL03153:Asap1 APN 15 64,032,123 (GRCm39) missense probably damaging 1.00
A4554:Asap1 UTSW 15 63,996,560 (GRCm39) splice site probably benign
PIT4378001:Asap1 UTSW 15 64,007,697 (GRCm39) missense probably damaging 0.99
R0081:Asap1 UTSW 15 63,971,413 (GRCm39) missense probably damaging 1.00
R0555:Asap1 UTSW 15 63,966,213 (GRCm39) missense probably damaging 1.00
R1414:Asap1 UTSW 15 64,030,733 (GRCm39) missense possibly damaging 0.92
R1437:Asap1 UTSW 15 63,991,956 (GRCm39) missense probably damaging 0.96
R1474:Asap1 UTSW 15 63,991,869 (GRCm39) missense probably benign 0.01
R1489:Asap1 UTSW 15 64,044,579 (GRCm39) missense probably damaging 1.00
R1553:Asap1 UTSW 15 64,024,701 (GRCm39) missense probably benign 0.31
R1603:Asap1 UTSW 15 64,001,106 (GRCm39) missense probably damaging 1.00
R1636:Asap1 UTSW 15 63,995,761 (GRCm39) missense probably damaging 1.00
R1645:Asap1 UTSW 15 63,961,324 (GRCm39) missense probably damaging 0.99
R1861:Asap1 UTSW 15 64,007,647 (GRCm39) splice site probably benign
R2136:Asap1 UTSW 15 63,982,808 (GRCm39) missense probably damaging 1.00
R2351:Asap1 UTSW 15 64,007,653 (GRCm39) critical splice donor site probably null
R4436:Asap1 UTSW 15 64,221,692 (GRCm39) missense probably benign 0.03
R4618:Asap1 UTSW 15 64,024,744 (GRCm39) missense probably damaging 1.00
R4868:Asap1 UTSW 15 63,966,030 (GRCm39) missense probably benign
R5077:Asap1 UTSW 15 63,999,272 (GRCm39) missense probably damaging 1.00
R5333:Asap1 UTSW 15 63,999,263 (GRCm39) missense possibly damaging 0.79
R5391:Asap1 UTSW 15 63,965,901 (GRCm39) missense possibly damaging 0.57
R5493:Asap1 UTSW 15 64,002,000 (GRCm39) missense possibly damaging 0.85
R5665:Asap1 UTSW 15 64,184,302 (GRCm39) missense probably damaging 1.00
R5756:Asap1 UTSW 15 64,039,556 (GRCm39) missense probably damaging 1.00
R5790:Asap1 UTSW 15 63,966,114 (GRCm39) missense probably damaging 1.00
R6139:Asap1 UTSW 15 64,038,388 (GRCm39) missense possibly damaging 0.87
R6194:Asap1 UTSW 15 64,001,058 (GRCm39) missense probably damaging 1.00
R6361:Asap1 UTSW 15 64,221,672 (GRCm39) splice site probably null
R6751:Asap1 UTSW 15 63,966,261 (GRCm39) missense possibly damaging 0.86
R7143:Asap1 UTSW 15 64,063,377 (GRCm39) missense probably damaging 1.00
R7218:Asap1 UTSW 15 64,002,099 (GRCm39) missense probably damaging 1.00
R7225:Asap1 UTSW 15 64,002,099 (GRCm39) missense probably damaging 1.00
R7305:Asap1 UTSW 15 64,002,099 (GRCm39) missense probably damaging 1.00
R7310:Asap1 UTSW 15 63,971,379 (GRCm39) critical splice donor site probably null
R7439:Asap1 UTSW 15 64,002,105 (GRCm39) missense probably damaging 1.00
R7441:Asap1 UTSW 15 64,002,105 (GRCm39) missense probably damaging 1.00
R7488:Asap1 UTSW 15 63,991,974 (GRCm39) missense probably benign 0.29
R7597:Asap1 UTSW 15 64,184,304 (GRCm39) missense probably benign 0.37
R7708:Asap1 UTSW 15 64,024,721 (GRCm39) missense probably damaging 1.00
R7871:Asap1 UTSW 15 63,963,925 (GRCm39) missense probably damaging 1.00
R7990:Asap1 UTSW 15 64,044,586 (GRCm39) splice site probably null
R8163:Asap1 UTSW 15 63,963,899 (GRCm39) missense probably damaging 1.00
R8171:Asap1 UTSW 15 63,982,815 (GRCm39) missense probably damaging 1.00
R8778:Asap1 UTSW 15 63,999,258 (GRCm39) missense probably benign 0.02
R9462:Asap1 UTSW 15 64,038,328 (GRCm39) missense probably damaging 0.98
Predicted Primers PCR Primer
(F):5'- TGTCTAAGTTGGCTCCCAAAC -3'
(R):5'- TTGACATTTTGGGGAGAGCAC -3'

Sequencing Primer
(F):5'- GTTGGCTCCCAAACTCCAAGG -3'
(R):5'- CATTTTGGGGAGAGCACTTAAG -3'
Posted On 2020-10-20