Incidental Mutation 'R8421:Prss21'
ID 653223
Institutional Source Beutler Lab
Gene Symbol Prss21
Ensembl Gene ENSMUSG00000024116
Gene Name serine protease 21
Synonyms TESP5, mT4, 1700023E12Rik, testisin
MMRRC Submission 067898-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.050) question?
Stock # R8421 (G1)
Quality Score 225.009
Status Validated
Chromosome 17
Chromosomal Location 24087046-24092087 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 24088342 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 102 (D102E)
Ref Sequence ENSEMBL: ENSMUSP00000024928 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000024928]
AlphaFold Q9JHJ7
Predicted Effect possibly damaging
Transcript: ENSMUST00000024928
AA Change: D102E

PolyPhen 2 Score 0.924 (Sensitivity: 0.81; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000024928
Gene: ENSMUSG00000024116
AA Change: D102E

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
Tryp_SPc 54 291 1.18e-94 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.5%
Validation Efficiency 98% (56/57)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. The encoded protein is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The encoded protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and may be involved in progression of testicular tumors of germ cell origin. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
PHENOTYPE: Mice homozygous for one knock-out allele impaires fertilization of epididymal sperm only in an in vitro experiment. Mice homozygous for another knock-out allele exhibit defective sperm maturation during passage through the epididymis and decreased spermfertilization capability. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acin1 T C 14: 54,880,486 (GRCm39) E1239G unknown Het
Actn3 T C 19: 4,911,741 (GRCm39) M806V probably benign Het
Adcy7 A G 8: 89,048,812 (GRCm39) T676A probably benign Het
Ankef1 C A 2: 136,379,085 (GRCm39) Q12K probably damaging Het
Ankrd40 G T 11: 94,225,662 (GRCm39) G231V probably damaging Het
Atad5 T A 11: 79,985,384 (GRCm39) V157D probably damaging Het
Atp6v1b1 T A 6: 83,730,791 (GRCm39) M163K probably damaging Het
Atp7b A T 8: 22,518,487 (GRCm39) M117K probably benign Het
Ccdc87 T C 19: 4,891,313 (GRCm39) Y602H possibly damaging Het
Cdh16 C T 8: 105,348,602 (GRCm39) R142K probably benign Het
Chrnb3 G A 8: 27,886,718 (GRCm39) V431I probably damaging Het
Csf1r A G 18: 61,260,966 (GRCm39) D719G probably damaging Het
Ctsll3 A G 13: 60,948,595 (GRCm39) F88S probably damaging Het
Dglucy A G 12: 100,808,938 (GRCm39) Y212C probably damaging Het
Dnase1l3 T C 14: 7,968,122 (GRCm38) D261G possibly damaging Het
Dync2h1 T C 9: 7,102,477 (GRCm39) D2641G probably damaging Het
Fat3 A T 9: 15,909,480 (GRCm39) V2174D probably damaging Het
Gtf3c1 A G 7: 125,298,142 (GRCm39) L244P probably damaging Het
Hcn4 T A 9: 58,765,379 (GRCm39) D620E unknown Het
Ide A G 19: 37,255,403 (GRCm39) V800A Het
Ikbkb A T 8: 23,168,804 (GRCm39) probably null Het
Lin9 T A 1: 180,493,365 (GRCm39) F197Y probably damaging Het
Lrp1b T A 2: 40,615,435 (GRCm39) Y3656F Het
Mapkbp1 C A 2: 119,849,431 (GRCm39) N708K probably damaging Het
Marveld3 A G 8: 110,675,279 (GRCm39) M179T probably benign Het
Mrpl1 C G 5: 96,374,226 (GRCm39) A167G probably benign Het
Mrpl35 T C 6: 71,793,151 (GRCm39) K167E probably damaging Het
Myo3a T C 2: 22,366,935 (GRCm39) V594A probably benign Het
Nav2 A G 7: 49,102,269 (GRCm39) T356A probably benign Het
Nup210l A G 3: 90,111,174 (GRCm39) Y1692C probably damaging Het
Or8k22 T A 2: 86,163,247 (GRCm39) Y151F possibly damaging Het
Otoa G A 7: 120,698,491 (GRCm39) probably null Het
Oxnad1 T G 14: 31,821,431 (GRCm39) I172S probably benign Het
Padi4 C A 4: 140,475,533 (GRCm39) C544F probably damaging Het
Pard3 A C 8: 127,867,158 (GRCm39) probably benign Het
Plvap G A 8: 71,964,176 (GRCm39) T62M probably damaging Het
Potefam1 T A 2: 111,048,955 (GRCm39) K275* probably null Het
Prdm8 C T 5: 98,333,822 (GRCm39) A463V probably damaging Het
Psmb10 G T 8: 106,663,342 (GRCm39) Q182K probably benign Het
Rasgrf1 C T 9: 89,849,968 (GRCm39) P319S probably damaging Het
Rock1 G A 18: 10,072,863 (GRCm39) Q1161* probably null Het
Ryr3 T C 2: 112,826,929 (GRCm39) E112G probably benign Het
Samm50 A G 15: 84,094,786 (GRCm39) T393A probably benign Het
Slc9c1 A G 16: 45,413,734 (GRCm39) E954G probably damaging Het
Sulf2 T A 2: 165,958,972 (GRCm39) I79F probably benign Het
Tbx21 T C 11: 97,005,561 (GRCm39) K135E probably benign Het
Ube2q2l T C 6: 136,378,350 (GRCm39) E160G probably damaging Het
Unc13b A G 4: 43,178,304 (GRCm39) E3044G unknown Het
Usf3 A T 16: 44,037,572 (GRCm39) Q684L possibly damaging Het
Vmn1r114 A T 7: 20,545,459 (GRCm39) M218K possibly damaging Het
Vmn1r203 T G 13: 22,709,154 (GRCm39) *312G probably null Het
Vmn2r67 A C 7: 84,785,893 (GRCm39) L704R probably damaging Het
Zfp618 A G 4: 63,051,483 (GRCm39) T755A probably damaging Het
Zp3 A G 5: 136,017,331 (GRCm39) T381A probably benign Het
Zscan20 T C 4: 128,479,620 (GRCm39) D957G probably damaging Het
Other mutations in Prss21
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01921:Prss21 APN 17 24,091,414 (GRCm39) missense possibly damaging 0.48
IGL03151:Prss21 APN 17 24,088,376 (GRCm39) missense probably damaging 0.98
R1136:Prss21 UTSW 17 24,091,968 (GRCm39) missense probably damaging 1.00
R2299:Prss21 UTSW 17 24,088,563 (GRCm39) missense probably benign 0.18
R3615:Prss21 UTSW 17 24,091,805 (GRCm39) missense probably benign 0.20
R3616:Prss21 UTSW 17 24,091,805 (GRCm39) missense probably benign 0.20
R4589:Prss21 UTSW 17 24,091,796 (GRCm39) missense possibly damaging 0.96
R5691:Prss21 UTSW 17 24,087,759 (GRCm39) splice site probably null
R6946:Prss21 UTSW 17 24,087,138 (GRCm39) missense possibly damaging 0.92
R7835:Prss21 UTSW 17 24,088,425 (GRCm39) missense possibly damaging 0.57
R8243:Prss21 UTSW 17 24,088,376 (GRCm39) missense probably damaging 0.98
Predicted Primers PCR Primer
(F):5'- AGGCTGACTCACCTTCTGTC -3'
(R):5'- GTGACTGGAGATGACAGCTTC -3'

Sequencing Primer
(F):5'- TGTCTTTAGGACTACTCCAACAGAC -3'
(R):5'- TGACAGCTTCAGCAGGGCTATG -3'
Posted On 2020-10-20