Mutagenetix > Incidental Mutations

Incidental Mutation 'R8422:Zfp810'

653258

Institutional Source

Beutler Lab

Gene Symbol

Zfp810

Ensembl Gene

ENSMUSG00000066829

Gene Name

zinc finger protein 810

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R8422 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

22276748-22307648 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 22283222 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 57 (E57*)

Ref Sequence

ENSEMBL: ENSMUSP00000083459 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086278] [ENSMUST00000215202]

AlphaFold

Q99K45

Predicted Effect

probably null
Transcript: ENSMUST00000086278
AA Change: E57*

SMART Domains

Protein: ENSMUSP00000083459
Gene: ENSMUSG00000066829
AA Change: E57*

Domain	Start	End	E-Value	Type
KRAB	4	64	1.09e-33	SMART
ZnF_C2H2	126	148	2.44e2	SMART
ZnF_C2H2	182	204	3.07e-1	SMART
ZnF_C2H2	210	232	8.47e-4	SMART
ZnF_C2H2	238	260	6.78e-3	SMART
ZnF_C2H2	266	288	6.13e-1	SMART
ZnF_C2H2	294	316	5.06e-2	SMART
ZnF_C2H2	322	344	4.79e-3	SMART
ZnF_C2H2	350	372	2.99e-4	SMART
ZnF_C2H2	378	400	1.33e-1	SMART
ZnF_C2H2	406	428	2.75e-3	SMART
ZnF_C2H2	434	456	1.58e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000215202
AA Change: E57*

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	G	T	14: 32,662,297	S570R	possibly damaging	Het
4921517D22Rik	T	C	13: 59,691,629	M1V	probably null	Het
4921524J17Rik	T	G	8: 85,412,289	K57T	possibly damaging	Het
4930596D02Rik	G	T	14: 35,810,052	Q189K	probably benign	Het
Anapc1	T	C	2: 128,675,837	T296A	probably benign	Het
Aqp7	T	A	4: 41,035,622	M78L	probably benign	Het
C1qtnf5	C	A	9: 44,108,664	A195E	possibly damaging	Het
Calhm2	T	C	19: 47,133,140	I197V	probably benign	Het
Ccdc186	G	A	19: 56,813,185	L167F	probably benign	Het
Ccr3	T	C	9: 124,028,762	Y45H	probably damaging	Het
Cct3	G	C	3: 88,300,819	R38P	probably damaging	Het
Clstn2	T	C	9: 97,458,186	D711G	probably benign	Het
Dab2ip	T	A	2: 35,707,755	I157N	probably damaging	Het
Dchs2	T	C	3: 83,325,263	V2185A	probably benign	Het
Dis3l2	A	G	1: 86,854,377	T219A	probably benign	Het
F2rl3	G	T	8: 72,763,185	V347L	probably benign	Het
Fastkd1	A	G	2: 69,702,434	S530P	probably damaging	Het
Fgfr3	T	A	5: 33,734,905	Y689*	probably null	Het
Gm10521	A	G	1: 171,896,459	I112M	unknown	Het
Gm9736	C	A	10: 77,750,880	V159F	unknown	Het
Hba-x	A	T	11: 32,277,736	H88L	probably benign	Het
Il12rb2	A	T	6: 67,360,816	V27E	probably benign	Het
Imp4	G	T	1: 34,443,916	G196V	probably damaging	Het
Itga11	A	C	9: 62,767,678	I831L	probably benign	Het
Macf1	C	T	4: 123,409,486	V408I	possibly damaging	Het
Nedd4	T	A	9: 72,742,682	D771E	probably damaging	Het
Noc3l	T	C	19: 38,807,103	Y413C	probably benign	Het
Npas3	C	A	12: 54,068,509	T738K	probably benign	Het
Ntrk2	A	T	13: 58,985,901	D498V	probably damaging	Het
Nyap1	T	C	5: 137,735,821	T317A	probably benign	Het
Olfr1212	T	C	2: 88,958,997	M177T	probably benign	Het
Olfr294	T	A	7: 86,616,258	H129L	probably benign	Het
Olfr74	T	A	2: 87,973,799	I289L	probably benign	Het
Olfr978	T	C	9: 39,994,554	L248P	probably damaging	Het
Pcdhb20	A	T	18: 37,504,796	D125V	probably damaging	Het
Phc3	G	A	3: 30,929,890	Q692*	probably null	Het
Plat	G	T	8: 22,772,232	G91W	probably damaging	Het
Ppp1r12a	T	A	10: 108,241,181	H339Q	probably benign	Het
Ppp1r3a	G	A	6: 14,718,435	Q827*	probably null	Het
Ptpra	T	A	2: 130,532,171	I265N	possibly damaging	Het
Rabggta	G	T	14: 55,718,458	H447Q	probably benign	Het
Ralgps1	T	C	2: 33,172,430	D277G	possibly damaging	Het
Riok3	A	T	18: 12,136,812	E100V	probably null	Het
Rtcb	C	T	10: 85,943,304	V362M	probably benign	Het
Scd2	G	T	19: 44,301,304	C246F	probably benign	Het
Slco6c1	T	C	1: 97,125,783	E131G	probably damaging	Het
Tanc2	A	G	11: 105,835,188	M393V	probably benign	Het
Tas2r136	A	T	6: 132,777,327	I279N	probably damaging	Het
Tbx3	G	T	5: 119,680,516	K405N	possibly damaging	Het
Thumpd2	A	T	17: 81,026,944	V405D	probably damaging	Het
Tlr12	A	G	4: 128,616,634	S608P	probably damaging	Het
Wfs1	T	C	5: 36,973,875	K165E	probably benign	Het

Other mutations in Zfp810

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00484:Zfp810	APN	9	22278309	nonsense	probably null
IGL03079:Zfp810	APN	9	22284127	missense	probably damaging	1.00
IGL03402:Zfp810	APN	9	22279145	splice site	probably null
H8562:Zfp810	UTSW	9	22279091	missense	probably benign	0.42
R1116:Zfp810	UTSW	9	22279085	missense	probably benign	0.11
R1160:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R1171:Zfp810	UTSW	9	22278826	missense	possibly damaging	0.95
R1393:Zfp810	UTSW	9	22280514	missense	probably benign
R1608:Zfp810	UTSW	9	22278920	missense	probably benign	0.00
R1644:Zfp810	UTSW	9	22279028	missense	possibly damaging	0.67
R1766:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R2568:Zfp810	UTSW	9	22279238	missense	probably benign	0.01
R3684:Zfp810	UTSW	9	22278235	missense	probably benign	0.01
R4002:Zfp810	UTSW	9	22278892	missense	probably damaging	1.00
R4134:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4135:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4334:Zfp810	UTSW	9	22278784	missense	probably benign	0.00
R4545:Zfp810	UTSW	9	22278745	missense	probably damaging	0.96
R5399:Zfp810	UTSW	9	22278829	missense	possibly damaging	0.91
R5622:Zfp810	UTSW	9	22279096	missense	probably benign	0.00
R5643:Zfp810	UTSW	9	22283171	missense	probably benign	0.26
R7375:Zfp810	UTSW	9	22290537	critical splice donor site	probably null
R7441:Zfp810	UTSW	9	22279272	nonsense	probably null
R7809:Zfp810	UTSW	9	22278982	missense	possibly damaging	0.51
R8526:Zfp810	UTSW	9	22278290	missense	probably damaging	1.00
R8719:Zfp810	UTSW	9	22279275	missense	probably benign	0.00
R9177:Zfp810	UTSW	9	22278640	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTGGTGGTTTAACGCACAGAAC -3'
(R):5'- GAGTCATAGGACAATGTGTTTGC -3'

Sequencing Primer
(F):5'- CGCACAGAACTTTTGGAAAACTG -3'
(R):5'- GGACAATGTGTTTGCTCAAATATGG -3'

Posted On

2020-10-20