Incidental Mutation 'R8423:Cnr2'
ID 653296
Institutional Source Beutler Lab
Gene Symbol Cnr2
Ensembl Gene ENSMUSG00000062585
Gene Name cannabinoid receptor 2
Synonyms cannabinoid receptor 2 (spleen), CB2-R, CB2
MMRRC Submission 067899-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.072) question?
Stock # R8423 (G1)
Quality Score 225.009
Status Validated
Chromosome 4
Chromosomal Location 135622705-135647518 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to T at 135644827 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Tryptophan at position 302 (R302W)
Ref Sequence ENSEMBL: ENSMUSP00000069957 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000030434] [ENSMUST00000068830] [ENSMUST00000097843]
AlphaFold P47936
Predicted Effect probably benign
Transcript: ENSMUST00000030434
SMART Domains Protein: ENSMUSP00000030434
Gene: ENSMUSG00000028673

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
Alpha_L_fucos 22 399 7.97e-234 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000068830
AA Change: R302W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000069957
Gene: ENSMUSG00000062585
AA Change: R302W

DomainStartEndE-ValueType
Pfam:7tm_1 50 299 1.8e-44 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000097843
AA Change: R302W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000095454
Gene: ENSMUSG00000062585
AA Change: R302W

DomainStartEndE-ValueType
Pfam:7tm_1 50 299 8e-37 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.6%
Validation Efficiency 98% (46/47)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Macrophages from homozygous mutant animals are resistant to the inhibitory effects of delta9-Tetrahydrocannabinol. Alopecia is seen in some but not all homozygotes. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam6b A G 12: 113,454,530 (GRCm39) D449G possibly damaging Het
Ampd1 T C 3: 102,988,305 (GRCm39) S83P probably benign Het
Bub1 G A 2: 127,649,750 (GRCm39) L703F probably benign Het
C1rb T A 6: 124,551,617 (GRCm39) D242E probably damaging Het
Carf T A 1: 60,189,752 (GRCm39) L659Q possibly damaging Het
Carmil3 A G 14: 55,736,522 (GRCm39) N662S probably damaging Het
Ccno A G 13: 113,124,678 (GRCm39) S83G possibly damaging Het
Chd3 A T 11: 69,245,252 (GRCm39) Y1155* probably null Het
Clec16a A G 16: 10,394,527 (GRCm39) E295G probably benign Het
Clnk A G 5: 38,952,253 (GRCm39) probably null Het
Clvs2 T C 10: 33,498,855 (GRCm39) D25G possibly damaging Het
Col20a1 A G 2: 180,640,498 (GRCm39) T594A probably damaging Het
Dclk3 C T 9: 111,297,787 (GRCm39) R444W possibly damaging Het
Dmxl1 T C 18: 49,998,183 (GRCm39) V761A probably damaging Het
Dnah7a C A 1: 53,512,063 (GRCm39) K2999N possibly damaging Het
Dusp28 T C 1: 92,834,906 (GRCm39) S44P probably damaging Het
Enpep T G 3: 129,102,774 (GRCm39) N394T probably damaging Het
Fam135a A T 1: 24,060,998 (GRCm39) F1343I probably damaging Het
Fbxl12 C A 9: 20,550,160 (GRCm39) R165L possibly damaging Het
Fbxo40 A T 16: 36,790,947 (GRCm39) H54Q probably damaging Het
Galnt9 A G 5: 110,744,111 (GRCm39) D315G probably benign Het
Gbp4 T A 5: 105,267,800 (GRCm39) D482V probably damaging Het
Gm17175 T A 14: 51,809,070 (GRCm39) I99F possibly damaging Het
Heatr4 A G 12: 84,027,104 (GRCm39) F51S probably benign Het
Herc1 T C 9: 66,415,442 (GRCm39) S4806P probably damaging Het
Hoxc11 G A 15: 102,863,177 (GRCm39) V73I possibly damaging Het
Knl1 T G 2: 118,900,513 (GRCm39) I738S probably damaging Het
Lcor T C 19: 41,573,888 (GRCm39) I881T possibly damaging Het
Lipt2 T C 7: 99,809,450 (GRCm39) F179S probably damaging Het
Lrrc8b G A 5: 105,628,622 (GRCm39) V323M probably damaging Het
Ltbp1 T C 17: 75,599,852 (GRCm39) S665P probably benign Het
Miga1 A T 3: 152,028,045 (GRCm39) D161E probably benign Het
Mynn A G 3: 30,657,933 (GRCm39) T84A probably benign Het
Or13c7d T C 4: 43,770,598 (GRCm39) S138G possibly damaging Het
Or3a1b T C 11: 74,012,492 (GRCm39) F126L probably benign Het
Or4a75 A T 2: 89,448,331 (GRCm39) D68E probably damaging Het
Pik3r3 C T 4: 116,079,572 (GRCm39) S23L probably benign Het
Plat G T 8: 23,262,248 (GRCm39) G91W probably damaging Het
Plscr4 C T 9: 92,372,843 (GRCm39) R322* probably null Het
Ralgapa1 A G 12: 55,705,847 (GRCm39) S1892P probably damaging Het
Slc6a18 T A 13: 73,813,693 (GRCm39) Q540L probably benign Het
Tfap2a A T 13: 40,872,706 (GRCm39) N323K possibly damaging Het
Tgm1 A T 14: 55,943,100 (GRCm39) M580K probably benign Het
Tnrc6b G A 15: 80,813,619 (GRCm39) G1792D unknown Het
Trim66 T A 7: 109,074,599 (GRCm39) Q554L possibly damaging Het
Ttc28 G A 5: 111,381,207 (GRCm39) D1240N probably benign Het
Vmn1r238 G T 18: 3,123,365 (GRCm39) C16* probably null Het
Vmn1r6 G A 6: 56,979,495 (GRCm39) M52I probably benign Het
Zfp61 A G 7: 23,991,459 (GRCm39) F231L probably benign Het
Other mutations in Cnr2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01990:Cnr2 APN 4 135,644,116 (GRCm39) missense probably damaging 1.00
IGL02233:Cnr2 APN 4 135,644,522 (GRCm39) missense possibly damaging 0.76
IGL02887:Cnr2 APN 4 135,644,936 (GRCm39) missense possibly damaging 0.90
PIT4791001:Cnr2 UTSW 4 135,644,263 (GRCm39) missense probably damaging 1.00
R0480:Cnr2 UTSW 4 135,644,912 (GRCm39) missense probably benign 0.00
R0616:Cnr2 UTSW 4 135,644,873 (GRCm39) missense probably benign 0.39
R0945:Cnr2 UTSW 4 135,644,632 (GRCm39) missense probably benign 0.16
R1242:Cnr2 UTSW 4 135,644,294 (GRCm39) missense probably damaging 1.00
R1538:Cnr2 UTSW 4 135,644,012 (GRCm39) missense probably benign 0.00
R4330:Cnr2 UTSW 4 135,644,237 (GRCm39) missense possibly damaging 0.56
R4911:Cnr2 UTSW 4 135,644,512 (GRCm39) missense possibly damaging 0.88
R4960:Cnr2 UTSW 4 135,644,918 (GRCm39) missense probably benign 0.01
R5289:Cnr2 UTSW 4 135,644,318 (GRCm39) missense probably damaging 1.00
R5642:Cnr2 UTSW 4 135,644,076 (GRCm39) missense probably damaging 1.00
R5681:Cnr2 UTSW 4 135,644,000 (GRCm39) missense probably damaging 0.99
R5807:Cnr2 UTSW 4 135,644,747 (GRCm39) missense probably benign 0.00
R6843:Cnr2 UTSW 4 135,644,900 (GRCm39) missense probably benign
R8024:Cnr2 UTSW 4 135,644,196 (GRCm39) missense probably damaging 1.00
R9330:Cnr2 UTSW 4 135,644,312 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TAGACGTGAGGTTGGCCAAG -3'
(R):5'- CTGAACGGACTTCTGACTCG -3'

Sequencing Primer
(F):5'- AGGTTGGCCAAGACTCTGG -3'
(R):5'- TCGGGCTGTTTCCAGTAGAAAGAC -3'
Posted On 2020-10-20