Incidental Mutation 'R8423:Trim66'
ID 653306
Institutional Source Beutler Lab
Gene Symbol Trim66
Ensembl Gene ENSMUSG00000031026
Gene Name tripartite motif-containing 66
Synonyms Tif1d, D7H11orf29
MMRRC Submission 067899-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.169) question?
Stock # R8423 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 109048213-109107341 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 109074599 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamine to Leucine at position 554 (Q554L)
Ref Sequence ENSEMBL: ENSMUSP00000102352 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033339] [ENSMUST00000106739] [ENSMUST00000106741]
AlphaFold Q924W6
Predicted Effect possibly damaging
Transcript: ENSMUST00000033339
AA Change: Q452L

PolyPhen 2 Score 0.768 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000033339
Gene: ENSMUSG00000031026
AA Change: Q452L

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000106739
AA Change: Q452L

PolyPhen 2 Score 0.768 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000102350
Gene: ENSMUSG00000031026
AA Change: Q452L

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000106741
AA Change: Q554L

PolyPhen 2 Score 0.768 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000102352
Gene: ENSMUSG00000031026
AA Change: Q554L

DomainStartEndE-ValueType
RING 28 78 2.38e-2 SMART
BBOX 102 140 1.48e0 SMART
PHD 106 171 7.77e0 SMART
RING 107 170 4.38e0 SMART
BBOX 162 203 4.21e-3 SMART
BBC 210 336 1.61e-39 SMART
low complexity region 420 435 N/A INTRINSIC
low complexity region 554 588 N/A INTRINSIC
low complexity region 619 632 N/A INTRINSIC
low complexity region 670 683 N/A INTRINSIC
PHD 1100 1143 4.09e-10 SMART
BROMO 1171 1277 8.22e-27 SMART
low complexity region 1287 1301 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.6%
Validation Efficiency 98% (46/47)
Allele List at MGI
Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam6b A G 12: 113,454,530 (GRCm39) D449G possibly damaging Het
Ampd1 T C 3: 102,988,305 (GRCm39) S83P probably benign Het
Bub1 G A 2: 127,649,750 (GRCm39) L703F probably benign Het
C1rb T A 6: 124,551,617 (GRCm39) D242E probably damaging Het
Carf T A 1: 60,189,752 (GRCm39) L659Q possibly damaging Het
Carmil3 A G 14: 55,736,522 (GRCm39) N662S probably damaging Het
Ccno A G 13: 113,124,678 (GRCm39) S83G possibly damaging Het
Chd3 A T 11: 69,245,252 (GRCm39) Y1155* probably null Het
Clec16a A G 16: 10,394,527 (GRCm39) E295G probably benign Het
Clnk A G 5: 38,952,253 (GRCm39) probably null Het
Clvs2 T C 10: 33,498,855 (GRCm39) D25G possibly damaging Het
Cnr2 C T 4: 135,644,827 (GRCm39) R302W probably damaging Het
Col20a1 A G 2: 180,640,498 (GRCm39) T594A probably damaging Het
Dclk3 C T 9: 111,297,787 (GRCm39) R444W possibly damaging Het
Dmxl1 T C 18: 49,998,183 (GRCm39) V761A probably damaging Het
Dnah7a C A 1: 53,512,063 (GRCm39) K2999N possibly damaging Het
Dusp28 T C 1: 92,834,906 (GRCm39) S44P probably damaging Het
Enpep T G 3: 129,102,774 (GRCm39) N394T probably damaging Het
Fam135a A T 1: 24,060,998 (GRCm39) F1343I probably damaging Het
Fbxl12 C A 9: 20,550,160 (GRCm39) R165L possibly damaging Het
Fbxo40 A T 16: 36,790,947 (GRCm39) H54Q probably damaging Het
Galnt9 A G 5: 110,744,111 (GRCm39) D315G probably benign Het
Gbp4 T A 5: 105,267,800 (GRCm39) D482V probably damaging Het
Gm17175 T A 14: 51,809,070 (GRCm39) I99F possibly damaging Het
Heatr4 A G 12: 84,027,104 (GRCm39) F51S probably benign Het
Herc1 T C 9: 66,415,442 (GRCm39) S4806P probably damaging Het
Hoxc11 G A 15: 102,863,177 (GRCm39) V73I possibly damaging Het
Knl1 T G 2: 118,900,513 (GRCm39) I738S probably damaging Het
Lcor T C 19: 41,573,888 (GRCm39) I881T possibly damaging Het
Lipt2 T C 7: 99,809,450 (GRCm39) F179S probably damaging Het
Lrrc8b G A 5: 105,628,622 (GRCm39) V323M probably damaging Het
Ltbp1 T C 17: 75,599,852 (GRCm39) S665P probably benign Het
Miga1 A T 3: 152,028,045 (GRCm39) D161E probably benign Het
Mynn A G 3: 30,657,933 (GRCm39) T84A probably benign Het
Or13c7d T C 4: 43,770,598 (GRCm39) S138G possibly damaging Het
Or3a1b T C 11: 74,012,492 (GRCm39) F126L probably benign Het
Or4a75 A T 2: 89,448,331 (GRCm39) D68E probably damaging Het
Pik3r3 C T 4: 116,079,572 (GRCm39) S23L probably benign Het
Plat G T 8: 23,262,248 (GRCm39) G91W probably damaging Het
Plscr4 C T 9: 92,372,843 (GRCm39) R322* probably null Het
Ralgapa1 A G 12: 55,705,847 (GRCm39) S1892P probably damaging Het
Slc6a18 T A 13: 73,813,693 (GRCm39) Q540L probably benign Het
Tfap2a A T 13: 40,872,706 (GRCm39) N323K possibly damaging Het
Tgm1 A T 14: 55,943,100 (GRCm39) M580K probably benign Het
Tnrc6b G A 15: 80,813,619 (GRCm39) G1792D unknown Het
Ttc28 G A 5: 111,381,207 (GRCm39) D1240N probably benign Het
Vmn1r238 G T 18: 3,123,365 (GRCm39) C16* probably null Het
Vmn1r6 G A 6: 56,979,495 (GRCm39) M52I probably benign Het
Zfp61 A G 7: 23,991,459 (GRCm39) F231L probably benign Het
Other mutations in Trim66
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01539:Trim66 APN 7 109,054,273 (GRCm39) missense probably benign 0.02
IGL01758:Trim66 APN 7 109,085,252 (GRCm39) critical splice donor site probably null
IGL01982:Trim66 APN 7 109,057,970 (GRCm39) missense probably benign 0.00
IGL01983:Trim66 APN 7 109,057,458 (GRCm39) nonsense probably null
IGL02149:Trim66 APN 7 109,060,109 (GRCm39) missense possibly damaging 0.66
IGL02392:Trim66 APN 7 109,059,481 (GRCm39) missense probably benign 0.01
IGL02483:Trim66 APN 7 109,076,837 (GRCm39) splice site probably benign
IGL02832:Trim66 APN 7 109,059,704 (GRCm39) missense probably damaging 1.00
IGL02945:Trim66 APN 7 109,059,383 (GRCm39) nonsense probably null
IGL03085:Trim66 APN 7 109,057,952 (GRCm39) missense probably benign 0.17
PIT1430001:Trim66 UTSW 7 109,074,454 (GRCm39) missense probably damaging 0.99
R0326:Trim66 UTSW 7 109,059,379 (GRCm39) missense probably benign 0.00
R0358:Trim66 UTSW 7 109,059,383 (GRCm39) nonsense probably null
R0401:Trim66 UTSW 7 109,074,471 (GRCm39) missense probably damaging 0.98
R0470:Trim66 UTSW 7 109,056,749 (GRCm39) splice site probably benign
R0568:Trim66 UTSW 7 109,059,902 (GRCm39) missense probably benign 0.00
R0669:Trim66 UTSW 7 109,054,199 (GRCm39) intron probably benign
R0980:Trim66 UTSW 7 109,054,877 (GRCm39) missense probably damaging 1.00
R1015:Trim66 UTSW 7 109,054,440 (GRCm39) missense probably damaging 1.00
R1078:Trim66 UTSW 7 109,071,526 (GRCm39) missense probably damaging 1.00
R1099:Trim66 UTSW 7 109,074,661 (GRCm39) missense probably benign 0.34
R1181:Trim66 UTSW 7 109,083,784 (GRCm39) critical splice donor site probably null
R1497:Trim66 UTSW 7 109,083,826 (GRCm39) missense probably benign 0.00
R1583:Trim66 UTSW 7 109,054,287 (GRCm39) missense probably damaging 1.00
R1843:Trim66 UTSW 7 109,075,046 (GRCm39) missense probably damaging 0.99
R1998:Trim66 UTSW 7 109,083,784 (GRCm39) critical splice donor site probably null
R2016:Trim66 UTSW 7 109,071,439 (GRCm39) critical splice donor site probably null
R2143:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R2144:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R2145:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R3945:Trim66 UTSW 7 109,071,475 (GRCm39) missense possibly damaging 0.94
R4012:Trim66 UTSW 7 109,057,338 (GRCm39) missense probably damaging 0.98
R4464:Trim66 UTSW 7 109,076,897 (GRCm39) missense possibly damaging 0.51
R4473:Trim66 UTSW 7 109,081,202 (GRCm39) missense probably damaging 1.00
R4729:Trim66 UTSW 7 109,055,267 (GRCm39) critical splice donor site probably null
R4730:Trim66 UTSW 7 109,082,276 (GRCm39) missense probably damaging 1.00
R4775:Trim66 UTSW 7 109,056,796 (GRCm39) nonsense probably null
R4819:Trim66 UTSW 7 109,056,793 (GRCm39) missense probably damaging 1.00
R5269:Trim66 UTSW 7 109,056,797 (GRCm39) missense probably benign 0.00
R5557:Trim66 UTSW 7 109,082,944 (GRCm39) missense probably benign 0.06
R5832:Trim66 UTSW 7 109,054,409 (GRCm39) missense probably damaging 1.00
R6220:Trim66 UTSW 7 109,082,300 (GRCm39) missense probably damaging 0.97
R6243:Trim66 UTSW 7 109,059,481 (GRCm39) missense probably benign 0.01
R6374:Trim66 UTSW 7 109,085,269 (GRCm39) missense probably benign
R6450:Trim66 UTSW 7 109,059,945 (GRCm39) missense probably benign 0.09
R6543:Trim66 UTSW 7 109,075,086 (GRCm39) missense probably benign 0.01
R6788:Trim66 UTSW 7 109,076,961 (GRCm39) missense probably damaging 1.00
R6842:Trim66 UTSW 7 109,059,983 (GRCm39) missense probably benign 0.00
R7169:Trim66 UTSW 7 109,054,328 (GRCm39) missense probably benign 0.25
R7257:Trim66 UTSW 7 109,059,451 (GRCm39) missense probably damaging 1.00
R7328:Trim66 UTSW 7 109,056,958 (GRCm39) missense probably damaging 0.99
R7616:Trim66 UTSW 7 109,082,956 (GRCm39) missense probably damaging 0.99
R8855:Trim66 UTSW 7 109,081,188 (GRCm39) missense probably damaging 1.00
R9130:Trim66 UTSW 7 109,076,896 (GRCm39) missense possibly damaging 0.90
R9137:Trim66 UTSW 7 109,074,330 (GRCm39) missense probably damaging 0.99
R9640:Trim66 UTSW 7 109,074,825 (GRCm39) missense probably damaging 1.00
RF013:Trim66 UTSW 7 109,059,960 (GRCm39) missense probably damaging 0.99
RF024:Trim66 UTSW 7 109,059,947 (GRCm39) missense possibly damaging 0.62
Predicted Primers PCR Primer
(F):5'- TGACTCAACTGCAGGCTAGG -3'
(R):5'- GAACAGGAGAGTACATCCCAGC -3'

Sequencing Primer
(F):5'- GAGAAGCTCCAAGTCCTTCTG -3'
(R):5'- AGTACATCCCAGCGGCCAG -3'
Posted On 2020-10-20