Incidental Mutation 'R8424:Zfp352'
| ID |
653344 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp352
|
| Ensembl Gene |
ENSMUSG00000070902 |
| Gene Name |
zinc finger protein 352 |
| Synonyms |
2czf48 |
| MMRRC Submission |
067818-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R8424 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
4 |
| Chromosomal Location |
90218820-90225702 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to T
at 90224243 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Proline to Serine
at position 207
(P207S)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000102746
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000080541]
[ENSMUST00000107129]
|
| AlphaFold |
A2AML7 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000080541
AA Change: P207S
PolyPhen 2
Score 0.874 (Sensitivity: 0.83; Specificity: 0.93)
|
| SMART Domains |
Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: P207S
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
459 |
483 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
489 |
513 |
8.22e-2 |
SMART |
|
ZnF_C2H2
|
519 |
542 |
1.76e-1 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000107129
AA Change: P207S
PolyPhen 2
Score 0.874 (Sensitivity: 0.83; Specificity: 0.93)
|
| SMART Domains |
Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: P207S
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
459 |
483 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
489 |
513 |
8.22e-2 |
SMART |
|
ZnF_C2H2
|
519 |
542 |
1.76e-1 |
SMART |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.7%
- 20x: 98.9%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 50 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abhd2 |
C |
T |
7: 79,297,137 |
P42S |
probably damaging |
Het |
| Acss2 |
A |
G |
2: 155,574,618 |
T694A |
unknown |
Het |
| Adar |
C |
T |
3: 89,735,994 |
P394L |
probably damaging |
Het |
| Alx4 |
A |
G |
2: 93,677,469 |
M370V |
probably benign |
Het |
| Bag6 |
T |
A |
17: 35,146,854 |
L1089Q |
probably damaging |
Het |
| Bms1 |
T |
C |
6: 118,388,760 |
Y1155C |
probably benign |
Het |
| Cbl |
G |
A |
9: 44,152,854 |
T795I |
possibly damaging |
Het |
| Cd300ld2 |
CGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGTCAGAACTGTGGATGGCACAACTGTGCATGGCAGAACTGTGGATGGCACAACTGTGGATGGCAGAACTGTGG |
CGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGTCAGAACTGTGGATGGCACAACTGTGCATGGCAGAACTGTGGATGGCACAACTGTGGATGGCAGAACTGTGG |
11: 115,012,431 |
|
probably benign |
Het |
| Cdc5l |
C |
A |
17: 45,415,600 |
A349S |
probably benign |
Het |
| Cdh5 |
G |
A |
8: 104,129,371 |
R312H |
probably benign |
Het |
| Cdk5rap1 |
T |
C |
2: 154,346,012 |
T465A |
probably damaging |
Het |
| Cep135 |
C |
T |
5: 76,594,059 |
T114I |
possibly damaging |
Het |
| Csrp1 |
T |
C |
1: 135,739,450 |
V17A |
probably damaging |
Het |
| Cutal |
T |
C |
2: 34,887,792 |
S105P |
probably benign |
Het |
| Dgki |
C |
A |
6: 36,850,915 |
V1016L |
probably benign |
Het |
| Dnmt1 |
T |
C |
9: 20,918,540 |
N746S |
probably benign |
Het |
| Ep400 |
A |
T |
5: 110,693,278 |
V1796E |
unknown |
Het |
| Fam83a |
T |
A |
15: 58,009,650 |
S292T |
possibly damaging |
Het |
| Hist1h2ab |
A |
G |
13: 23,751,284 |
Y51C |
probably damaging |
Het |
| Keap1 |
C |
A |
9: 21,230,790 |
R596L |
probably benign |
Het |
| Klhl5 |
A |
C |
5: 65,162,962 |
T620P |
probably benign |
Het |
| Lbhd1 |
T |
A |
19: 8,883,977 |
D16E |
possibly damaging |
Het |
| Nadk2 |
T |
C |
15: 9,083,334 |
V110A |
possibly damaging |
Het |
| Ncbp1 |
A |
T |
4: 46,144,839 |
H30L |
probably benign |
Het |
| Olfr203 |
A |
T |
16: 59,303,409 |
L85F |
possibly damaging |
Het |
| Plat |
G |
T |
8: 22,772,232 |
G91W |
probably damaging |
Het |
| Pmp22 |
G |
T |
11: 63,133,076 |
|
probably benign |
Het |
| Rc3h1 |
C |
T |
1: 160,965,772 |
L1076F |
probably damaging |
Het |
| Ryr3 |
T |
C |
2: 112,841,894 |
I1431V |
possibly damaging |
Het |
| S100a7a |
T |
A |
3: 90,655,561 |
H18Q |
probably damaging |
Het |
| Scd2 |
G |
T |
19: 44,301,304 |
C246F |
probably benign |
Het |
| Serpina1c |
T |
C |
12: 103,896,037 |
T340A |
possibly damaging |
Het |
| Setd5 |
A |
G |
6: 113,149,683 |
E1227G |
probably benign |
Het |
| Sh3yl1 |
G |
A |
12: 30,924,863 |
R71H |
probably damaging |
Het |
| Spry2 |
A |
G |
14: 105,893,402 |
S117P |
probably damaging |
Het |
| Srcap |
T |
C |
7: 127,542,388 |
V1780A |
probably benign |
Het |
| Tas2r130 |
T |
G |
6: 131,630,827 |
T2P |
probably benign |
Het |
| Tiam2 |
T |
A |
17: 3,516,041 |
F1454I |
probably damaging |
Het |
| Tiam2 |
T |
C |
17: 3,516,042 |
F1454S |
probably damaging |
Het |
| Tmem173 |
A |
C |
18: 35,739,170 |
I93S |
probably benign |
Het |
| Tnpo3 |
A |
G |
6: 29,555,206 |
S793P |
probably benign |
Het |
| Ttc28 |
G |
A |
5: 111,233,341 |
D1240N |
probably benign |
Het |
| Ubap2l |
A |
T |
3: 90,021,031 |
D535E |
probably damaging |
Het |
| Ube2h |
A |
G |
6: 30,260,941 |
S65P |
probably damaging |
Het |
| Vmn1r203 |
T |
C |
13: 22,524,834 |
S262P |
probably damaging |
Het |
| Wfdc8 |
A |
G |
2: 164,603,158 |
F179S |
probably benign |
Het |
| Wnk1 |
T |
C |
6: 119,934,427 |
T2153A |
unknown |
Het |
| Zfhx3 |
G |
T |
8: 108,856,753 |
G1084V |
probably damaging |
Het |
| Zfp273 |
A |
G |
13: 67,822,352 |
N40D |
probably benign |
Het |
| Zfp952 |
T |
A |
17: 33,003,217 |
F223L |
probably benign |
Het |
|
| Other mutations in Zfp352 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01963:Zfp352
|
APN |
4 |
90,224,154 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
IGL02252:Zfp352
|
APN |
4 |
90,224,130 (GRCm38) |
missense |
probably benign |
0.02 |
|
IGL03156:Zfp352
|
APN |
4 |
90,224,087 (GRCm38) |
missense |
possibly damaging |
0.57 |
|
IGL03167:Zfp352
|
APN |
4 |
90,224,702 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL03190:Zfp352
|
APN |
4 |
90,223,757 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
IGL03335:Zfp352
|
APN |
4 |
90,224,346 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0051:Zfp352
|
UTSW |
4 |
90,224,285 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0403:Zfp352
|
UTSW |
4 |
90,225,009 (GRCm38) |
missense |
possibly damaging |
0.60 |
|
R0550:Zfp352
|
UTSW |
4 |
90,224,690 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0671:Zfp352
|
UTSW |
4 |
90,223,919 (GRCm38) |
missense |
probably benign |
|
|
R1034:Zfp352
|
UTSW |
4 |
90,224,156 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R1754:Zfp352
|
UTSW |
4 |
90,223,809 (GRCm38) |
missense |
probably benign |
0.23 |
|
R2016:Zfp352
|
UTSW |
4 |
90,225,171 (GRCm38) |
missense |
probably benign |
0.42 |
|
R2064:Zfp352
|
UTSW |
4 |
90,225,120 (GRCm38) |
missense |
probably benign |
0.08 |
|
R2308:Zfp352
|
UTSW |
4 |
90,225,243 (GRCm38) |
missense |
probably benign |
0.00 |
|
R3552:Zfp352
|
UTSW |
4 |
90,225,102 (GRCm38) |
missense |
probably benign |
0.33 |
|
R3794:Zfp352
|
UTSW |
4 |
90,225,149 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3795:Zfp352
|
UTSW |
4 |
90,225,149 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4135:Zfp352
|
UTSW |
4 |
90,225,024 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R4356:Zfp352
|
UTSW |
4 |
90,223,834 (GRCm38) |
missense |
possibly damaging |
0.91 |
|
R4409:Zfp352
|
UTSW |
4 |
90,225,164 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4590:Zfp352
|
UTSW |
4 |
90,224,535 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4614:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4617:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4618:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4741:Zfp352
|
UTSW |
4 |
90,224,940 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R4931:Zfp352
|
UTSW |
4 |
90,224,304 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4959:Zfp352
|
UTSW |
4 |
90,224,139 (GRCm38) |
missense |
probably benign |
0.01 |
|
R4973:Zfp352
|
UTSW |
4 |
90,224,139 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5167:Zfp352
|
UTSW |
4 |
90,224,216 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R5260:Zfp352
|
UTSW |
4 |
90,224,460 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R5524:Zfp352
|
UTSW |
4 |
90,225,104 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
R5942:Zfp352
|
UTSW |
4 |
90,225,070 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R6802:Zfp352
|
UTSW |
4 |
90,225,200 (GRCm38) |
missense |
probably benign |
0.33 |
|
R6819:Zfp352
|
UTSW |
4 |
90,224,699 (GRCm38) |
missense |
probably benign |
|
|
R7072:Zfp352
|
UTSW |
4 |
90,224,424 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7099:Zfp352
|
UTSW |
4 |
90,224,880 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7569:Zfp352
|
UTSW |
4 |
90,223,659 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R7645:Zfp352
|
UTSW |
4 |
90,224,777 (GRCm38) |
missense |
probably benign |
0.13 |
|
R7705:Zfp352
|
UTSW |
4 |
90,225,275 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R9180:Zfp352
|
UTSW |
4 |
90,224,881 (GRCm38) |
missense |
probably benign |
0.38 |
|
R9378:Zfp352
|
UTSW |
4 |
90,224,338 (GRCm38) |
missense |
probably benign |
0.13 |
|
R9509:Zfp352
|
UTSW |
4 |
90,224,706 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R9623:Zfp352
|
UTSW |
4 |
90,224,891 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- ACAGCCATGTGTGAGAATCAG -3'
(R):5'- TGACCACTGAAGGATTTCATTTGG -3'
Sequencing Primer
(F):5'- CATGTGTGAGAATCAGAGGGTTACC -3'
(R):5'- GTTTGGTCAAAGTTAGCTCTCATC -3'
|
| Posted On |
2020-10-20 |
Incidental Mutation