Mutagenetix > Incidental Mutation

Incidental Mutation 'R8428:Ifi44'

653577

Institutional Source

Beutler Lab

Gene Symbol

Ifi44

Ensembl Gene

ENSMUSG00000028037

Gene Name

interferon-induced protein 44

Synonyms

A430056A10Rik, p44, MTAP44

MMRRC Submission

067822-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8428 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

151436559-151455580 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 151444978 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 325 (R325*)

Ref Sequence

ENSEMBL: ENSMUSP00000029671 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029671]

AlphaFold

Q8BV66

Predicted Effect

probably null
Transcript: ENSMUST00000029671
AA Change: R325*

SMART Domains

Protein: ENSMUSP00000029671
Gene: ENSMUSG00000028037
AA Change: R325*

Domain	Start	End	E-Value	Type
Pfam:TLD	26	147	2.8e-7	PFAM
low complexity region	193	208	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agap2	A	G	10: 126,923,175 (GRCm39)	I670V	unknown	Het
Bcan	G	T	3: 87,904,405 (GRCm39)	T117K	probably damaging	Het
Bhlhe40	TG	TGG	6: 108,641,818 (GRCm39)	254	probably null	Het
Cbx8	C	A	11: 118,929,754 (GRCm39)	V280F	probably damaging	Het
Ccdc117	A	T	11: 5,484,350 (GRCm39)	S163R	possibly damaging	Het
Ccdc17	A	T	4: 116,456,823 (GRCm39)	I509F	probably damaging	Het
Cdon	T	C	9: 35,403,163 (GRCm39)	V1091A	probably benign	Het
Cntnap5b	C	T	1: 100,311,310 (GRCm39)	T972I	probably damaging	Het
Dlg2	T	C	7: 90,740,240 (GRCm39)	L9P	possibly damaging	Het
Dnah6	T	A	6: 73,051,634 (GRCm39)	R3053S	probably benign	Het
Dnah7a	T	A	1: 53,512,112 (GRCm39)	N2983I	probably damaging	Het
Dnah7c	T	C	1: 46,711,536 (GRCm39)	Y2588H	probably damaging	Het
Dpysl5	A	G	5: 30,902,811 (GRCm39)	D81G	probably damaging	Het
Dscaml1	A	G	9: 45,653,884 (GRCm39)	D1387G	probably benign	Het
Exd1	G	T	2: 119,369,348 (GRCm39)	T89K	possibly damaging	Het
Ezh2	T	A	6: 47,522,745 (GRCm39)	R364*	probably null	Het
Fam161b	C	T	12: 84,404,369 (GRCm39)	D104N	probably benign	Het
Fbxw8	C	A	5: 118,215,763 (GRCm39)	V416L	probably benign	Het
Flnc	A	T	6: 29,450,849 (GRCm39)	D1499V	probably benign	Het
Fmc1	A	T	6: 38,516,115 (GRCm39)	R54*	probably null	Het
Fzr1	A	G	10: 81,206,942 (GRCm39)	F61S	probably damaging	Het
Get4	T	G	5: 139,251,393 (GRCm39)	C160G	probably benign	Het
Gm32742	G	A	9: 51,055,675 (GRCm39)	R1330*	probably null	Het
Gnat3	G	A	5: 18,220,312 (GRCm39)	A225T	possibly damaging	Het
Gucy2c	A	G	6: 136,704,892 (GRCm39)	Y541H	probably damaging	Het
Hand2	A	G	8: 57,775,461 (GRCm39)	T174A	probably benign	Het
Helb	T	C	10: 119,927,522 (GRCm39)	T863A	probably damaging	Het
Hoxa7	A	T	6: 52,194,993 (GRCm39)	V2D	unknown	Het
Igfn1	C	T	1: 135,895,520 (GRCm39)	G1682E	probably damaging	Het
Itch	A	G	2: 155,010,627 (GRCm39)	N32D	probably benign	Het
Kif26b	T	C	1: 178,744,923 (GRCm39)	V1673A	probably benign	Het
Map1a	A	T	2: 121,135,418 (GRCm39)	D2078V	probably benign	Het
Mgat4b	G	T	11: 50,121,512 (GRCm39)	V35L	probably benign	Het
Micu3	G	A	8: 40,761,205 (GRCm39)	M38I	probably benign	Het
Myo15a	A	T	11: 60,387,241 (GRCm39)	H706L	probably damaging	Het
Ndc1	A	G	4: 107,226,017 (GRCm39)	T42A	probably benign	Het
Nfat5	T	A	8: 108,095,152 (GRCm39)	M1131K	probably damaging	Het
Nop14	G	A	5: 34,798,784 (GRCm39)	S648L	probably damaging	Het
Or51k1	G	T	7: 103,661,632 (GRCm39)	Y92*	probably null	Het
Otogl	A	G	10: 107,634,597 (GRCm39)	V1413A	probably damaging	Het
Palb2	T	A	7: 121,711,224 (GRCm39)	M967L	possibly damaging	Het
Pira12	G	A	7: 3,898,257 (GRCm39)	T355I	probably benign	Het
Ppa2	A	G	3: 133,053,904 (GRCm39)	K198R	probably damaging	Het
Prokr1	T	C	6: 87,565,756 (GRCm39)	T30A	probably benign	Het
Prss50	C	T	9: 110,687,128 (GRCm39)	R24C	unknown	Het
Rbm17	T	C	2: 11,605,441 (GRCm39)	T38A	possibly damaging	Het
Rps6ka5	G	A	12: 100,541,500 (GRCm39)	Q420*	probably null	Het
Senp6	G	T	9: 80,025,794 (GRCm39)	R448L	probably damaging	Het
Senp7	T	C	16: 55,999,391 (GRCm39)	I838T	probably damaging	Het
Sf3b2	C	T	19: 5,337,242 (GRCm39)	S329N	possibly damaging	Het
Slc13a4	A	T	6: 35,245,814 (GRCm39)	D610E	probably benign	Het
Slc24a2	A	G	4: 87,145,337 (GRCm39)	L239P	probably damaging	Het
Slc39a5	T	A	10: 128,232,884 (GRCm39)	H389L	probably damaging	Het
Syt4	A	G	18: 31,577,072 (GRCm39)	L94P	probably damaging	Het
Tgm5	A	G	2: 120,879,356 (GRCm39)	V560A	probably benign	Het
Thoc5	A	G	11: 4,876,115 (GRCm39)	T623A	probably damaging	Het
Tm7sf2	C	T	19: 6,113,074 (GRCm39)	V376I	probably benign	Het
Tmem268	T	A	4: 63,496,141 (GRCm39)	V194E	probably damaging	Het
Tpr	C	A	1: 150,290,564 (GRCm39)	R798S	probably damaging	Het
Trpv5	G	T	6: 41,630,182 (GRCm39)	T685K	possibly damaging	Het
Ttn	A	T	2: 76,584,774 (GRCm39)	N22141K	probably damaging	Het
Txnrd2	T	C	16: 18,275,048 (GRCm39)	I353T	unknown	Het
Upk1a	A	G	7: 30,303,043 (GRCm39)	Y252H	probably damaging	Het
Vmn2r118	A	G	17: 55,915,642 (GRCm39)	I436T	probably benign	Het
Vmn2r76	T	A	7: 85,874,479 (GRCm39)	I833F	possibly damaging	Het
Zfp277	T	C	12: 40,379,577 (GRCm39)	H319R	probably damaging	Het

Other mutations in Ifi44

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00858:Ifi44	APN	3	151,455,217 (GRCm39)	missense	probably benign	0.03
IGL01477:Ifi44	APN	3	151,451,635 (GRCm39)	splice site	probably benign
IGL01571:Ifi44	APN	3	151,451,174 (GRCm39)	missense	probably damaging	0.97
IGL02165:Ifi44	APN	3	151,455,067 (GRCm39)	missense	probably damaging	1.00
IGL02238:Ifi44	APN	3	151,438,019 (GRCm39)	makesense	probably null
IGL02609:Ifi44	APN	3	151,438,134 (GRCm39)	missense	probably damaging	1.00
IGL02650:Ifi44	APN	3	151,451,492 (GRCm39)	missense	probably damaging	1.00
IGL02726:Ifi44	APN	3	151,455,233 (GRCm39)	start gained	probably benign
IGL02977:Ifi44	APN	3	151,445,016 (GRCm39)	missense	probably benign	0.00
R0201:Ifi44	UTSW	3	151,451,273 (GRCm39)	missense	probably damaging	1.00
R0454:Ifi44	UTSW	3	151,451,134 (GRCm39)	missense	possibly damaging	0.78
R0763:Ifi44	UTSW	3	151,455,135 (GRCm39)	missense	probably damaging	1.00
R1640:Ifi44	UTSW	3	151,438,171 (GRCm39)	missense	probably benign	0.00
R1747:Ifi44	UTSW	3	151,454,922 (GRCm39)	missense	probably benign	0.00
R2278:Ifi44	UTSW	3	151,438,025 (GRCm39)	missense	probably benign
R3816:Ifi44	UTSW	3	151,454,894 (GRCm39)	missense	possibly damaging	0.92
R4075:Ifi44	UTSW	3	151,451,613 (GRCm39)	missense	probably benign	0.00
R4084:Ifi44	UTSW	3	151,451,126 (GRCm39)	critical splice donor site	probably null
R4782:Ifi44	UTSW	3	151,451,229 (GRCm39)	missense	probably damaging	0.99
R5071:Ifi44	UTSW	3	151,455,269 (GRCm39)	start gained	probably benign
R5074:Ifi44	UTSW	3	151,455,269 (GRCm39)	start gained	probably benign
R6030:Ifi44	UTSW	3	151,455,195 (GRCm39)	missense	probably benign	0.00
R6030:Ifi44	UTSW	3	151,455,195 (GRCm39)	missense	probably benign	0.00
R6128:Ifi44	UTSW	3	151,454,823 (GRCm39)	missense	probably benign	0.00
R6192:Ifi44	UTSW	3	151,451,276 (GRCm39)	critical splice acceptor site	probably null
R6434:Ifi44	UTSW	3	151,454,826 (GRCm39)	missense	probably benign	0.02
R6536:Ifi44	UTSW	3	151,438,126 (GRCm39)	missense	probably benign	0.03
R6902:Ifi44	UTSW	3	151,451,536 (GRCm39)	missense	possibly damaging	0.92
R6946:Ifi44	UTSW	3	151,451,536 (GRCm39)	missense	possibly damaging	0.92
R7346:Ifi44	UTSW	3	151,438,094 (GRCm39)	missense	probably benign
R7608:Ifi44	UTSW	3	151,438,045 (GRCm39)	missense	probably damaging	0.97
R7704:Ifi44	UTSW	3	151,438,061 (GRCm39)	missense	probably benign	0.44
R7971:Ifi44	UTSW	3	151,454,857 (GRCm39)	missense	possibly damaging	0.89
R8255:Ifi44	UTSW	3	151,451,619 (GRCm39)	missense	probably benign	0.00
R8940:Ifi44	UTSW	3	151,454,946 (GRCm39)	missense	probably benign	0.00
R9087:Ifi44	UTSW	3	151,451,517 (GRCm39)	missense	probably damaging	1.00
R9259:Ifi44	UTSW	3	151,454,875 (GRCm39)	missense	possibly damaging	0.75
R9436:Ifi44	UTSW	3	151,454,886 (GRCm39)	missense	probably benign	0.00
R9516:Ifi44	UTSW	3	151,438,108 (GRCm39)	missense	probably damaging	0.97
R9619:Ifi44	UTSW	3	151,451,509 (GRCm39)	missense	probably damaging	0.97
Z1176:Ifi44	UTSW	3	151,438,090 (GRCm39)	missense	probably damaging	1.00
Z1177:Ifi44	UTSW	3	151,455,075 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCATGCAGCTGTTGAGAGC -3'
(R):5'- TTCAAATGCACAGGACTGGAG -3'

Sequencing Primer
(F):5'- CTCACCGAGATGTAACCATGGTAATG -3'
(R):5'- CAGGACTGGAGTATCATTTATAAGC -3'

Posted On

2020-10-20