Mutagenetix > Incidental Mutation

Incidental Mutation 'R8429:Mrgprb4'

653649

Institutional Source

Beutler Lab

Gene Symbol

Mrgprb4

Ensembl Gene

ENSMUSG00000070550

Gene Name

MAS-related GPR, member B4

Synonyms

MrgB4

MMRRC Submission

067774-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8429 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

48198070-48199288 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 48198425 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 252 (F252I)

Ref Sequence

ENSEMBL: ENSMUSP00000091952 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094388]

AlphaFold

Q91ZC0

Predicted Effect

probably benign
Transcript: ENSMUST00000094388
AA Change: F252I

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000091952
Gene: ENSMUSG00000070550
AA Change: F252I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	37	215	6.4e-8	PFAM
Pfam:7tm_1	46	274	7.6e-12	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele are viable, fertile and overtly normal with no apparent alterations in the pattern of axonal innervation of the epidermis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700122O11Rik	A	T	17: 48,037,066 (GRCm38)	L143*	probably null	Het
2900026A02Rik	T	C	5: 113,183,436 (GRCm38)	T971A	probably benign	Het
Abca6	A	T	11: 110,202,382 (GRCm38)	C1022S	probably benign	Het
Adgrf4	G	T	17: 42,667,449 (GRCm38)	N334K	probably benign	Het
Baz1b	A	G	5: 135,217,331 (GRCm38)	K545E	probably benign	Het
Bin3	A	G	14: 70,137,149 (GRCm38)	Y209C	probably damaging	Het
Btbd16	G	A	7: 130,795,337 (GRCm38)	A223T	probably benign	Het
C3	A	G	17: 57,222,811 (GRCm38)	V555A	probably damaging	Het
Calm3	T	A	7: 16,919,667 (GRCm38)		probably null	Het
Cct4	T	A	11: 22,996,030 (GRCm38)	L124Q	probably damaging	Het
Cenpf	C	T	1: 189,657,307 (GRCm38)	D1443N	possibly damaging	Het
Egfem1	G	A	3: 29,657,268 (GRCm38)		probably null	Het
Epha4	G	T	1: 77,390,036 (GRCm38)	Q591K	probably benign	Het
Fanci	T	C	7: 79,438,385 (GRCm38)	F929L	possibly damaging	Het
Fnip1	A	T	11: 54,475,696 (GRCm38)	D95V	possibly damaging	Het
Foxc1	A	T	13: 31,807,776 (GRCm38)	H190L	probably benign	Het
Gm45713	T	C	7: 45,136,116 (GRCm38)	S2G	unknown	Het
Grin2b	T	C	6: 135,733,916 (GRCm38)	I877M	probably damaging	Het
Hadha	T	C	5: 30,144,257 (GRCm38)	I119V	probably benign	Het
Hcar2	C	T	5: 123,865,475 (GRCm38)		probably benign	Het
Irag2	C	A	6: 145,165,223 (GRCm38)	D251E	probably damaging	Het
Krt13	A	T	11: 100,121,125 (GRCm38)	L124Q	probably damaging	Het
Larp1b	A	G	3: 40,977,227 (GRCm38)	*336W	probably null	Het
Man2c1	T	C	9: 57,131,161 (GRCm38)	L35P	probably damaging	Het
Meioc	A	T	11: 102,674,206 (GRCm38)	N160I	probably benign	Het
Mfsd2b	G	T	12: 4,866,487 (GRCm38)	Q331K	possibly damaging	Het
Mical2	T	A	7: 112,345,253 (GRCm38)	V930E	probably benign	Het
Nars1	A	G	18: 64,501,320 (GRCm38)	Y511H	probably damaging	Het
Naxe	C	A	3: 88,058,152 (GRCm38)	S84I	probably damaging	Het
Ncam2	A	T	16: 81,589,635 (GRCm38)	D634V	probably damaging	Het
Npat	C	T	9: 53,570,609 (GRCm38)	Q1206*	probably null	Het
Nr1d2	G	A	14: 18,215,409 (GRCm38)	T201I	probably benign	Het
Nt5el	A	G	13: 105,118,788 (GRCm38)	Y459C	probably damaging	Het
Nup155	C	T	15: 8,112,420 (GRCm38)	H99Y	probably damaging	Het
Or10ag60	G	T	2: 87,607,524 (GRCm38)	L45F	probably benign	Het
Or4f4b	G	A	2: 111,483,495 (GRCm38)	V50I	possibly damaging	Het
Or5ac25	A	G	16: 59,361,627 (GRCm38)	V197A	possibly damaging	Het
Or9k2b	A	G	10: 130,180,223 (GRCm38)	V219A	possibly damaging	Het
Pcnx3	C	T	19: 5,665,384 (GRCm38)	G1946E	probably damaging	Het
Pde6a	T	A	18: 61,232,844 (GRCm38)	Y214N	probably damaging	Het
Pex7	T	C	10: 19,894,328 (GRCm38)	T145A	probably damaging	Het
Plekhj1	C	T	10: 80,796,470 (GRCm38)	S146N	probably benign	Het
Ralgapb	C	T	2: 158,426,297 (GRCm38)	P107S	probably damaging	Het
Satb1	A	G	17: 51,767,950 (GRCm38)	M506T	probably damaging	Het
Sh3gl1	T	C	17: 56,018,821 (GRCm38)	N203D	possibly damaging	Het
Slc7a12	A	T	3: 14,497,282 (GRCm38)	I240F	probably benign	Het
Spata31h1	G	T	10: 82,289,467 (GRCm38)	Q2570K	possibly damaging	Het
Syt17	T	A	7: 118,434,341 (GRCm38)	Y144F	probably benign	Het
Thbd	G	T	2: 148,407,537 (GRCm38)	T137K	possibly damaging	Het
Tmem114	A	G	16: 8,412,167 (GRCm38)	F124L	probably damaging	Het
Ubtd1	G	T	19: 42,032,117 (GRCm38)		probably null	Het
Zfp458	A	G	13: 67,258,088 (GRCm38)	Y96H	possibly damaging	Het
Zfp78	T	C	7: 6,378,493 (GRCm38)	S181P	probably benign	Het

Other mutations in Mrgprb4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00769:Mrgprb4	APN	7	48,198,901 (GRCm38)	missense	probably benign
IGL02745:Mrgprb4	APN	7	48,198,358 (GRCm38)	missense	probably damaging	0.97
R0718:Mrgprb4	UTSW	7	48,198,553 (GRCm38)	missense	probably benign	0.10
R0849:Mrgprb4	UTSW	7	48,199,120 (GRCm38)	missense	probably benign	0.24
R1526:Mrgprb4	UTSW	7	48,198,411 (GRCm38)	nonsense	probably null
R2857:Mrgprb4	UTSW	7	48,198,336 (GRCm38)	missense	possibly damaging	0.91
R2859:Mrgprb4	UTSW	7	48,198,336 (GRCm38)	missense	possibly damaging	0.91
R4355:Mrgprb4	UTSW	7	48,198,701 (GRCm38)	missense	possibly damaging	0.63
R5354:Mrgprb4	UTSW	7	48,198,329 (GRCm38)	missense	probably benign	0.07
R5636:Mrgprb4	UTSW	7	48,198,470 (GRCm38)	missense	probably benign	0.02
R5715:Mrgprb4	UTSW	7	48,199,039 (GRCm38)	missense	probably damaging	1.00
R6180:Mrgprb4	UTSW	7	48,198,826 (GRCm38)	missense	probably damaging	1.00
R6277:Mrgprb4	UTSW	7	48,198,901 (GRCm38)	missense	probably benign
R7092:Mrgprb4	UTSW	7	48,198,236 (GRCm38)	missense	probably benign	0.01
R7301:Mrgprb4	UTSW	7	48,198,758 (GRCm38)	missense	probably damaging	0.99
R7779:Mrgprb4	UTSW	7	48,199,147 (GRCm38)	missense	probably benign	0.19
R8077:Mrgprb4	UTSW	7	48,198,455 (GRCm38)	missense	probably benign	0.00
R8292:Mrgprb4	UTSW	7	48,198,806 (GRCm38)	missense	probably damaging	1.00
R9000:Mrgprb4	UTSW	7	48,199,021 (GRCm38)	missense	probably damaging	1.00
R9006:Mrgprb4	UTSW	7	48,198,595 (GRCm38)	missense	probably benign	0.12
R9106:Mrgprb4	UTSW	7	48,198,931 (GRCm38)	missense	probably benign	0.43
R9717:Mrgprb4	UTSW	7	48,198,835 (GRCm38)	missense	possibly damaging	0.96
Z1088:Mrgprb4	UTSW	7	48,198,682 (GRCm38)	missense	possibly damaging	0.78

Predicted Primers

PCR Primer
(F):5'- GGGACTCACTCAACTACTGTACTAG -3'
(R):5'- TATCTAGCCTGGCCCTGTTG -3'

Sequencing Primer
(F):5'- AGTTCCCCAGACCTTTGCGAAG -3'
(R):5'- CCTGGCCCTGTTGGTGAAG -3'

Posted On

2020-10-20