Mutagenetix > Incidental Mutation

Incidental Mutation 'R8434:Zfp664'

653894

Institutional Source

Beutler Lab

Gene Symbol

Zfp664

Ensembl Gene

ENSMUSG00000079215

Gene Name

zinc finger protein 664

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8434 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

124862691-124902693 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 124885763 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 74 (L74F)

Ref Sequence

ENSEMBL: ENSMUSP00000107048 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111417]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000111417
AA Change: L74F

PolyPhen 2 Score 0.899 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000107048
Gene: ENSMUSG00000079215
AA Change: L74F

Domain	Start	End	E-Value	Type
ZnF_C2H2	3	25	2.57e-3	SMART
ZnF_C2H2	31	53	7.55e-1	SMART
ZnF_C2H2	59	81	5.99e-4	SMART
ZnF_C2H2	87	109	3.21e-4	SMART
ZnF_C2H2	115	137	4.54e-4	SMART
ZnF_C2H2	143	165	4.79e-3	SMART
ZnF_C2H2	171	193	8.34e-3	SMART
ZnF_C2H2	199	221	6.23e-2	SMART
ZnF_C2H2	227	249	7.9e-4	SMART

Meta Mutation Damage Score

0.4137

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (57/57)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 138,067,260	T737A	probably damaging	Het
2310034C09Rik	A	G	16: 88,759,372	Y158C	probably damaging	Het
Abhd18	T	A	3: 40,930,896	S239T	possibly damaging	Het
Ankrd13b	G	A	11: 77,477,726	T56I	probably benign	Het
Arhgef10l	T	A	4: 140,564,271	Q454L	possibly damaging	Het
Atp1a2	T	C	1: 172,284,612	E572G	probably benign	Het
Cdk5rap2	T	C	4: 70,364,020	H164R	probably benign	Het
Clca4b	A	G	3: 144,926,156	M196T	probably benign	Het
Cnbd1	T	G	4: 19,055,045	K127T	probably benign	Het
Cog3	A	T	14: 75,742,396	V165E	probably damaging	Het
Crim1	G	A	17: 78,347,257	R654H	probably benign	Het
Ctnnb1	G	A	9: 120,957,562	V617I	possibly damaging	Het
Dach1	T	A	14: 98,168,693	Q206L	probably damaging	Het
Dhx30	C	T	9: 110,100,906	V41I	probably benign	Het
Dnase2a	T	C	8: 84,909,781	L176P	probably damaging	Het
Dpp10	T	C	1: 123,433,010	D262G	probably damaging	Het
Dsel	T	A	1: 111,861,655	E383D	probably damaging	Het
Flt1	T	A	5: 147,639,443	Y585F	probably damaging	Het
Fubp1	T	A	3: 152,220,553	I304N	probably damaging	Het
Gab2	A	G	7: 97,299,130	D309G	probably damaging	Het
Gon4l	T	C	3: 88,854,779	V291A	probably damaging	Het
Gpam	C	T	19: 55,081,631	V398M	possibly damaging	Het
Grin3b	C	T	10: 79,974,588	R643C	probably damaging	Het
Hdac3	T	A	18: 37,941,422	H339L	possibly damaging	Het
Hsdl2	A	T	4: 59,610,621	Q326L	unknown	Het
Insr	T	C	8: 3,165,514		probably benign	Het
Ivl	T	A	3: 92,572,636	T41S	probably benign	Het
Lama4	C	T	10: 39,026,707	P226S	possibly damaging	Het
Lpin1	A	G	12: 16,563,620		probably null	Het
Lrrc42	A	T	4: 107,247,524	N81K	probably damaging	Het
Mast4	A	T	13: 102,761,392	H838Q	probably damaging	Het
Mcmdc2	A	G	1: 9,920,581	M314V	possibly damaging	Het
Me3	A	G	7: 89,739,878	E130G	probably damaging	Het
Mpnd	A	G	17: 56,009,405	D28G	possibly damaging	Het
Mtch2	G	T	2: 90,852,864	E102*	probably null	Het
Myh13	A	T	11: 67,363,185		probably null	Het
Olfr1117-ps1	G	A	2: 87,284,634	V115I	probably benign	Het
Olfr291	C	A	7: 84,857,289	H309N	probably benign	Het
Plch2	T	A	4: 154,989,735	D891V	probably damaging	Het
Ppfibp2	T	C	7: 107,728,750		probably null	Het
Rag1	A	G	2: 101,642,664	L711P	probably damaging	Het
Sacs	T	A	14: 61,213,187	Y4227*	probably null	Het
Sec23ip	C	T	7: 128,750,427	H176Y	probably benign	Het
Sema3a	T	C	5: 13,473,520		probably null	Het
Serpina10	C	T	12: 103,628,304	G219R	probably damaging	Het
Sp1	C	T	15: 102,409,683	L546F	probably benign	Het
Syne1	T	C	10: 5,123,057	N1256S	probably benign	Het
Tbc1d5	G	T	17: 50,782,427		probably benign	Het
Tcrg-V3	A	G	13: 19,242,866	T8A	probably benign	Het
Tns1	T	C	1: 73,925,606	S33G	probably benign	Het
Tpsab1	A	G	17: 25,345,471	L3P	possibly damaging	Het
Vav2	A	T	2: 27,269,038		probably benign	Het
Vmn1r44	T	C	6: 89,893,628	S119P	possibly damaging	Het
Vmn2r31	A	G	7: 7,384,700	L624P	probably damaging	Het
Vps33a	A	T	5: 123,533,881	W475R	possibly damaging	Het
Xpnpep3	G	T	15: 81,427,594	R167L	possibly damaging	Het
Zfhx4	T	A	3: 5,398,858	S1384T	probably damaging	Het
Zfp3	G	A	11: 70,772,558	E448K	probably benign	Het
Zfp362	T	A	4: 128,785,976	H299L	probably damaging	Het
Zfp808	A	G	13: 62,172,112	Y385C	probably damaging	Het

Other mutations in Zfp664

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01556:Zfp664	APN	5	124886188	nonsense	probably null
IGL02683:Zfp664	APN	5	124886322	missense	probably benign	0.03
R0394:Zfp664	UTSW	5	124886065	nonsense	probably null
R0629:Zfp664	UTSW	5	124885595	missense	probably damaging	0.99
R1400:Zfp664	UTSW	5	124886153	missense	unknown
R6052:Zfp664	UTSW	5	124886186	missense	unknown
R6058:Zfp664	UTSW	5	124885978	nonsense	probably null
R6529:Zfp664	UTSW	5	124886288	missense	probably damaging	1.00
R7031:Zfp664	UTSW	5	124886006	missense	probably benign	0.06
R7752:Zfp664	UTSW	5	124885775	nonsense	probably null
R7901:Zfp664	UTSW	5	124885775	nonsense	probably null
R8686:Zfp664	UTSW	5	124886069	missense	possibly damaging	0.61

Predicted Primers

PCR Primer
(F):5'- TACAAGTGCCCCATGTGCAG -3'
(R):5'- CTTTGATGCATGCAGAGGC -3'

Sequencing Primer
(F):5'- CCCATGTGCAGGGAATTTTTC -3'
(R):5'- ACTCTCTGGTGCATGCAGAG -3'

Posted On

2020-10-20