Mutagenetix > Incidental Mutation

Incidental Mutation 'R8441:Olfml1'

654209

Institutional Source

Beutler Lab

Gene Symbol

Olfml1

Ensembl Gene

ENSMUSG00000051041

Gene Name

olfactomedin-like 1

Synonyms

mONT2, ONT2, MVAL564

MMRRC Submission

067885-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R8441 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

107166653-107190301 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 107166977 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glycine at position 2 (V2G)

Ref Sequence

ENSEMBL: ENSMUSP00000114029 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000120990]

AlphaFold

Q8BSH2

Predicted Effect

probably benign
Transcript: ENSMUST00000120990
AA Change: V2G

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000114029
Gene: ENSMUSG00000051041
AA Change: V2G

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
coiled coil region	78	105	N/A	INTRINSIC
low complexity region	115	132	N/A	INTRINSIC
Pfam:OLF	145	233	5.5e-19	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	A	G	2: 69,087,574 (GRCm39)	Y1064H	possibly damaging	Het
Ahrr	T	C	13: 74,362,182 (GRCm39)	D567G	probably benign	Het
Aldh1b1	T	A	4: 45,802,465 (GRCm39)	M1K	probably null	Het
Ankrd12	A	T	17: 66,349,546 (GRCm39)	S96T	probably benign	Het
Arhgef10	A	T	8: 15,041,237 (GRCm39)		probably benign	Het
Bsn	G	A	9: 107,988,651 (GRCm39)	A2367V	probably benign	Het
Cmpk2	G	A	12: 26,527,204 (GRCm39)	A398T	probably benign	Het
Cpn2	A	G	16: 30,078,849 (GRCm39)	L284P	probably damaging	Het
Cubn	T	C	2: 13,432,658 (GRCm39)	D1221G	probably damaging	Het
Dnah7c	A	G	1: 46,572,398 (GRCm39)	K957R	probably damaging	Het
Eif1ad2	T	G	12: 87,786,384 (GRCm39)	D98E	probably benign	Het
Flnb	T	A	14: 7,896,488 (GRCm38)	V893E	probably benign	Het
Flrt3	C	A	2: 140,502,546 (GRCm39)	V361L	probably benign	Het
Fnta	G	A	8: 26,501,209 (GRCm39)	R104*	probably null	Het
Ggt1	C	T	10: 75,415,185 (GRCm39)	T233I	possibly damaging	Het
Gpr37l1	A	T	1: 135,094,875 (GRCm39)	V123E	probably damaging	Het
Grpel1	A	G	5: 36,622,556 (GRCm39)	R7G	probably benign	Het
H2-M10.5	A	T	17: 37,084,199 (GRCm39)	I54L	probably benign	Het
Mapk8ip3	A	T	17: 25,139,474 (GRCm39)		probably benign	Het
Mcm3	T	C	1: 20,884,690 (GRCm39)	D271G	probably benign	Het
Naip6	A	G	13: 100,422,265 (GRCm39)	V1256A	possibly damaging	Het
Nipbl	A	G	15: 8,322,599 (GRCm39)	V2604A	probably benign	Het
Nlrp1b	T	A	11: 71,073,204 (GRCm39)	D213V	probably damaging	Het
Npbwr1	G	A	1: 5,987,397 (GRCm39)	A39V	possibly damaging	Het
Nr6a1	T	C	2: 38,632,888 (GRCm39)	D191G	probably benign	Het
Or4k38	A	T	2: 111,166,131 (GRCm39)	Y97*	probably null	Het
Or5b105	T	A	19: 13,080,020 (GRCm39)	Y216F	probably damaging	Het
Otof	T	C	5: 30,538,200 (GRCm39)	K1175E	probably damaging	Het
Pira13	C	A	7: 3,826,301 (GRCm39)	E231*	probably null	Het
Plekhg2	C	T	7: 28,060,291 (GRCm39)	V989I	probably benign	Het
Prkcq	A	G	2: 11,253,037 (GRCm39)	D229G	probably benign	Het
Ptprf	A	T	4: 118,075,255 (GRCm39)		probably benign	Het
Rest	G	T	5: 77,429,766 (GRCm39)	Q728H	possibly damaging	Het
Scube1	A	T	15: 83,494,423 (GRCm39)	I868N	probably damaging	Het
Spcs3	A	G	8: 54,981,375 (GRCm39)		probably null	Het
Speg	A	G	1: 75,387,976 (GRCm39)	S1445G	possibly damaging	Het
Spmap2	C	A	10: 79,412,510 (GRCm39)	R327L	probably damaging	Het
Tmem145	T	C	7: 25,008,200 (GRCm39)	F261S	possibly damaging	Het
Trav9d-4	A	G	14: 53,221,284 (GRCm39)	S93G	probably benign	Het
Trbv5	T	A	6: 41,039,517 (GRCm39)	C41S	probably damaging	Het
Trpm5	A	G	7: 142,626,171 (GRCm39)	S1131P	possibly damaging	Het
Ttc28	C	T	5: 111,325,507 (GRCm39)	R313*	probably null	Het
Ubap2l	T	C	3: 89,920,007 (GRCm39)	T853A	unknown	Het
Xirp2	T	C	2: 67,343,159 (GRCm39)	V1800A	possibly damaging	Het
Zfhx2	A	G	14: 55,303,985 (GRCm39)	L1333P	possibly damaging	Het

Other mutations in Olfml1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Olfml1	APN	7	107,189,364 (GRCm39)	missense	possibly damaging	0.80
IGL02355:Olfml1	APN	7	107,167,010 (GRCm39)	missense	probably benign	0.00
IGL02362:Olfml1	APN	7	107,167,010 (GRCm39)	missense	probably benign	0.00
IGL03218:Olfml1	APN	7	107,170,476 (GRCm39)	missense	possibly damaging	0.87
IGL03291:Olfml1	APN	7	107,189,436 (GRCm39)	missense	probably damaging	1.00
R0041:Olfml1	UTSW	7	107,189,393 (GRCm39)	missense	possibly damaging	0.81
R0041:Olfml1	UTSW	7	107,189,393 (GRCm39)	missense	possibly damaging	0.81
R0081:Olfml1	UTSW	7	107,170,506 (GRCm39)	missense	probably benign	0.08
R0524:Olfml1	UTSW	7	107,189,384 (GRCm39)	missense	probably damaging	1.00
R1311:Olfml1	UTSW	7	107,167,103 (GRCm39)	critical splice donor site	probably null
R1548:Olfml1	UTSW	7	107,189,582 (GRCm39)	missense	possibly damaging	0.88
R1564:Olfml1	UTSW	7	107,170,346 (GRCm39)	missense	possibly damaging	0.89
R4347:Olfml1	UTSW	7	107,167,040 (GRCm39)	missense	probably benign	0.00
R4997:Olfml1	UTSW	7	107,170,413 (GRCm39)	missense	probably damaging	1.00
R6788:Olfml1	UTSW	7	107,167,075 (GRCm39)	missense	probably damaging	1.00
R7282:Olfml1	UTSW	7	107,189,530 (GRCm39)	missense	possibly damaging	0.85
R7703:Olfml1	UTSW	7	107,170,392 (GRCm39)	missense	probably damaging	1.00
R7922:Olfml1	UTSW	7	107,170,356 (GRCm39)	missense	probably damaging	1.00
R8324:Olfml1	UTSW	7	107,189,570 (GRCm39)	missense	probably benign	0.04
R9002:Olfml1	UTSW	7	107,189,423 (GRCm39)	missense	probably damaging	1.00
R9261:Olfml1	UTSW	7	107,167,007 (GRCm39)	missense	possibly damaging	0.88
R9797:Olfml1	UTSW	7	107,167,069 (GRCm39)	missense	probably benign	0.25

Predicted Primers

PCR Primer
(F):5'- AACTGAGATCATGCACCGGG -3'
(R):5'- CATTCTGTATCAGGAAGACATGCAC -3'

Sequencing Primer
(F):5'- TCATGCACCGGGTAGAATAAC -3'
(R):5'- TATCAGGAAGACATGCACCCTAGTTG -3'

Posted On

2020-10-20