Mutagenetix > Incidental Mutation

Incidental Mutation 'R8456:Naa16'

655043

Institutional Source

Beutler Lab

Gene Symbol

Naa16

Ensembl Gene

ENSMUSG00000022020

Gene Name

N(alpha)-acetyltransferase 16, NatA auxiliary subunit

Synonyms

Narg1l, 1300019C06Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R8456 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

79325269-79390778 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 79359475 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 356 (N356K)

Ref Sequence

ENSEMBL: ENSMUSP00000022597 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022597] [ENSMUST00000163486]

AlphaFold

Q9DBB4

Predicted Effect

probably benign
Transcript: ENSMUST00000022597
AA Change: N356K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000022597
Gene: ENSMUSG00000022020
AA Change: N356K

Domain	Start	End	E-Value	Type
TPR	46	79	2.99e1	SMART
TPR	80	113	2.98e-3	SMART
Blast:TPR	224	257	1e-10	BLAST
TPR	374	407	9.96e0	SMART
TPR	408	441	7.47e0	SMART
low complexity region	616	633	N/A	INTRINSIC
Blast:TPR	672	705	3e-12	BLAST
low complexity region	830	841	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000163486
AA Change: N322K

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000131268
Gene: ENSMUSG00000022020
AA Change: N322K

Domain	Start	End	E-Value	Type
TPR	12	45	2.99e1	SMART
TPR	46	79	2.98e-3	SMART
Blast:TPR	190	223	3e-10	BLAST
TPR	340	373	9.96e0	SMART
TPR	374	407	7.47e0	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca7	A	G	10: 80,006,526	E1098G	probably benign	Het
Akap1	T	A	11: 88,834,731		probably null	Het
Arhgap26	T	C	18: 39,111,848	V182A	possibly damaging	Het
Arhgef19	A	G	4: 141,250,615	K545R	probably benign	Het
Asph	C	T	4: 9,537,722	R352Q	probably benign	Het
B3glct	T	A	5: 149,726,789	I119N	probably damaging	Het
Camsap3	C	T	8: 3,600,679	R236*	probably null	Het
Ccser2	A	T	14: 36,890,374	M792K	probably benign	Het
Ceacam5	C	T	7: 17,745,699	T247I	possibly damaging	Het
Dnah9	A	G	11: 66,156,938	S19P	probably damaging	Het
Dtwd1	A	G	2: 126,158,531	E129G	probably damaging	Het
Ebf3	T	A	7: 137,199,187	M490L	probably benign	Het
Erbb4	A	G	1: 68,071,630	L1008S	probably damaging	Het
Ereg	C	T	5: 91,090,134	P160S	possibly damaging	Het
Exoc6b	ATTT	ATTTT	6: 84,844,095		probably null	Het
Fmn1	A	G	2: 113,365,040	T362A	unknown	Het
Gm10436	T	A	12: 88,177,216	T284S	probably benign	Het
Gm14326	A	T	2: 177,948,519	D16E	probably benign	Het
H1foo	A	T	6: 115,948,784	M181L	probably benign	Het
Herc6	A	T	6: 57,598,563	T190S	probably benign	Het
Hist1h3a	A	T	13: 23,762,100	F85Y	probably damaging	Het
Ikbkap	ACTTCTTCTTCTTCTTCTTCTTC	ACTTCTTCTTCTTCTTCTTC	4: 56,781,176		probably benign	Het
Lama1	T	G	17: 67,737,496	I130S		Het
Letm2	T	C	8: 25,581,713	D391G	probably damaging	Het
Map3k9	T	C	12: 81,734,118	I423V	probably damaging	Het
Melk	T	A	4: 44,312,191	C168S	possibly damaging	Het
Nup214	A	G	2: 32,039,360	N1873S	probably benign	Het
Olfr1392	C	T	11: 49,293,558	P79L	probably damaging	Het
Olfr890	T	A	9: 38,143,685	C178*	probably null	Het
Pom121	A	G	5: 135,381,178	F1042L	unknown	Het
Pxdn	T	C	12: 30,011,890	S1334P	probably damaging	Het
Ranbp1	C	T	16: 18,245,306	E69K	probably damaging	Het
Rasl11a	T	C	5: 146,845,235	S7P	probably damaging	Het
Rev1	G	A	1: 38,059,243	R740*	probably null	Het
Rnf157	C	T	11: 116,349,420	V443M	probably benign	Het
Scn3a	T	A	2: 65,460,673	T1910S	probably benign	Het
Sh2b1	TGGGGACCAGCTCAGCCACGGGGACCAGCTC	TGGGGACCAGCTCAGCCACGGGGACCAGCTCAGCCACGGGGACCAGCTC	7: 126,467,570		probably benign	Het
Sh2b1	C	CAGCCACGGGGCCTAGCTA	7: 126,467,600		probably null	Het
Siah2	T	C	3: 58,676,082	N261S	probably benign	Het
Sumf2	T	G	5: 129,860,162	W264G	possibly damaging	Het
Tal1	G	T	4: 115,063,428	A100S	probably benign	Het
Tmprss9	A	G	10: 80,894,417	D717G	possibly damaging	Het
Tox2	G	T	2: 163,204,630	R9L	unknown	Het
Traj46	A	G	14: 54,172,338	E1G		Het
Uhrf1bp1l	A	G	10: 89,812,092	T1339A	probably benign	Het
Unc80	A	G	1: 66,641,629	D2128G	possibly damaging	Het
Usp21	A	T	1: 171,284,717	F308I	probably damaging	Het
Vgf	A	T	5: 137,032,411	I476F	probably damaging	Het
Vps35	G	A	8: 85,261,305	T739I	possibly damaging	Het
Wdr95	G	T	5: 149,579,107	C279F	probably damaging	Het
Zbtb32	T	C	7: 30,589,956	S94G	unknown	Het
Zdhhc13	T	A	7: 48,802,999	I153N	probably damaging	Het

Other mutations in Naa16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Naa16	APN	14	79355729	missense	probably damaging	1.00
IGL01025:Naa16	APN	14	79384756	missense	probably damaging	1.00
IGL01155:Naa16	APN	14	79384715	missense	probably damaging	0.98
IGL01335:Naa16	APN	14	79345116	splice site	probably benign
IGL01981:Naa16	APN	14	79381516	missense	probably benign	0.05
IGL02230:Naa16	APN	14	79377361	splice site	probably benign
IGL02313:Naa16	APN	14	79384668	missense	probably damaging	1.00
IGL02418:Naa16	APN	14	79383366	missense	probably damaging	1.00
IGL02544:Naa16	APN	14	79335820	missense	probably damaging	1.00
IGL03051:Naa16	APN	14	79369082	missense	probably benign	0.01
IGL03064:Naa16	APN	14	79339628	missense	probably damaging	0.98
IGL03205:Naa16	APN	14	79356512	missense	possibly damaging	0.89
PIT4508001:Naa16	UTSW	14	79369087	missense	probably benign	0.15
R0651:Naa16	UTSW	14	79351392	missense	probably damaging	1.00
R1429:Naa16	UTSW	14	79359527	missense	probably benign	0.01
R1674:Naa16	UTSW	14	79387057	start codon destroyed	probably null	0.65
R1693:Naa16	UTSW	14	79351456	missense	probably damaging	1.00
R1874:Naa16	UTSW	14	79355743	missense	possibly damaging	0.62
R1992:Naa16	UTSW	14	79356491	missense	probably damaging	1.00
R2015:Naa16	UTSW	14	79345059	missense	probably damaging	1.00
R2391:Naa16	UTSW	14	79370049	missense	probably benign	0.16
R2847:Naa16	UTSW	14	79335883	missense	probably damaging	1.00
R2848:Naa16	UTSW	14	79335883	missense	probably damaging	1.00
R2877:Naa16	UTSW	14	79343298	missense	probably benign	0.00
R3884:Naa16	UTSW	14	79343262	missense	probably damaging	0.98
R4001:Naa16	UTSW	14	79343121	splice site	probably null
R4199:Naa16	UTSW	14	79355871	missense	probably damaging	1.00
R4638:Naa16	UTSW	14	79340033	splice site	probably null
R4676:Naa16	UTSW	14	79336348	unclassified	probably benign
R4690:Naa16	UTSW	14	79345057	missense	probably damaging	1.00
R4952:Naa16	UTSW	14	79345085	missense	probably damaging	1.00
R5087:Naa16	UTSW	14	79377415	missense	possibly damaging	0.68
R5104:Naa16	UTSW	14	79384700	nonsense	probably null
R5729:Naa16	UTSW	14	79355780	missense	probably damaging	1.00
R6178:Naa16	UTSW	14	79383340	missense	possibly damaging	0.93
R6960:Naa16	UTSW	14	79359471	missense	possibly damaging	0.65
R7794:Naa16	UTSW	14	79377494	missense	probably damaging	1.00
R7936:Naa16	UTSW	14	79341046	missense	possibly damaging	0.47
R8356:Naa16	UTSW	14	79359475	missense	probably benign	0.00
R8892:Naa16	UTSW	14	79390576	missense	probably benign	0.32
R8931:Naa16	UTSW	14	79344955	missense	probably damaging	1.00
R9010:Naa16	UTSW	14	79370042	missense	probably benign	0.01
R9068:Naa16	UTSW	14	79374849	missense	probably benign	0.18
R9360:Naa16	UTSW	14	79356503	missense	probably benign	0.05
R9688:Naa16	UTSW	14	79335869	nonsense	probably null
X0064:Naa16	UTSW	14	79351389	missense	probably damaging	1.00
Z1177:Naa16	UTSW	14	79344979	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAAGGCTAATTAACTCCCAGCC -3'
(R):5'- GAGGCACAGTTTCTACTTTCTG -3'

Sequencing Primer
(F):5'- GTAGTCATTTGTGTAGCACAC -3'
(R):5'- CTTAACTCCAGTTTCAGGGGG -3'

Posted On

2020-10-20