Mutagenetix > Incidental Mutation

Incidental Mutation 'R8501:Gpr165'

655351

Institutional Source

Beutler Lab

Gene Symbol

Gpr165

Ensembl Gene

ENSMUSG00000031210

Gene Name

G protein-coupled receptor 165

Synonyms

6530406P05Rik

MMRRC Submission

067838-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R8501 (G1)

Quality Score

221.999

Status

Not validated

Chromosome

Chromosomal Location

95757074-95762994 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 95757623 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 7 (D7E)

Ref Sequence

ENSEMBL: ENSMUSP00000033554 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033554]

AlphaFold

Q3V3A3

Predicted Effect

probably benign
Transcript: ENSMUST00000033554
AA Change: D7E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000033554
Gene: ENSMUSG00000031210
AA Change: D7E

Domain	Start	End	E-Value	Type
Pfam:7tm_1	112	369	3e-40	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5031439G07Rik	C	T	15: 84,844,724 (GRCm39)	V98M	probably damaging	Het
Agbl2	G	A	2: 90,627,908 (GRCm39)	G238R	probably damaging	Het
Atg2a	T	C	19: 6,304,420 (GRCm39)	S1146P	probably damaging	Het
Atg3	C	A	16: 45,003,294 (GRCm39)	D207E	probably damaging	Het
Atp8b3	A	G	10: 80,355,980 (GRCm39)	V1244A	probably benign	Het
Cacna1i	T	C	15: 80,266,247 (GRCm39)		probably null	Het
Ccdc171	G	T	4: 83,581,895 (GRCm39)	E675*	probably null	Het
Cep68	A	T	11: 20,189,132 (GRCm39)	S627T	unknown	Het
Chrnd	T	C	1: 87,120,338 (GRCm39)	W165R	probably damaging	Het
Col6a2	G	T	10: 76,439,391 (GRCm39)	A791D	probably damaging	Het
Dlg2	C	A	7: 92,024,930 (GRCm39)	S598R	probably damaging	Het
Elfn2	G	T	15: 78,558,500 (GRCm39)	Q16K	probably benign	Het
Gm40460	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 141,794,450 (GRCm39)		probably benign	Het
Grm8	A	T	6: 27,618,540 (GRCm39)	M434K	probably damaging	Het
Idh2	TCCCAGG	T	7: 79,748,079 (GRCm39)		probably benign	Het
Igf2	G	C	7: 142,207,779 (GRCm39)	P106R	probably damaging	Het
Klf7	T	C	1: 64,118,322 (GRCm39)	S92G	probably benign	Het
Mme	T	A	3: 63,234,156 (GRCm39)	M73K	probably damaging	Het
Musk	G	C	4: 58,367,502 (GRCm39)	K588N	probably damaging	Het
Naa30	C	A	14: 49,410,353 (GRCm39)	H94N	possibly damaging	Het
Naip6	C	T	13: 100,436,784 (GRCm39)	A580T	possibly damaging	Het
Nfya	A	G	17: 48,706,017 (GRCm39)	L52P	unknown	Het
Or14j5	A	G	17: 38,161,756 (GRCm39)	Y91C	probably damaging	Het
Or1j17	A	G	2: 36,578,809 (GRCm39)	N265S	probably benign	Het
Or51b6	T	A	7: 103,555,818 (GRCm39)	H54Q		Het
Otogl	A	T	10: 107,626,421 (GRCm39)	M1481K	probably benign	Het
P4ha3	A	G	7: 99,962,562 (GRCm39)	D440G	probably damaging	Het
Pcdhb13	A	T	18: 37,577,493 (GRCm39)	T624S	probably damaging	Het
Phf8-ps	C	A	17: 33,286,038 (GRCm39)	A255S	probably benign	Het
Piezo2	A	G	18: 63,178,611 (GRCm39)	Y1769H	probably damaging	Het
Plekha6	T	A	1: 133,215,575 (GRCm39)	V913E	probably benign	Het
Rapgefl1	A	G	11: 98,733,053 (GRCm39)	E211G	possibly damaging	Het
Rcc2	T	C	4: 140,443,237 (GRCm39)	L305P	probably damaging	Het
Rgs3	G	T	4: 62,521,193 (GRCm39)	V47F	possibly damaging	Het
Scly	A	G	1: 91,246,798 (GRCm39)	S370G	probably damaging	Het
Setmar	T	A	6: 108,052,822 (GRCm39)	D105E	probably benign	Het
Slc9a9	A	G	9: 94,737,792 (GRCm39)	Y233C	probably damaging	Het
Sp140	A	T	1: 85,569,461 (GRCm39)	N357I	probably damaging	Het
Trim30c	G	C	7: 104,036,677 (GRCm39)	T227R	probably benign	Het
Unc79	G	A	12: 103,058,897 (GRCm39)	V1014I	probably damaging	Het
Vmn1r151	A	T	7: 22,199,034 (GRCm39)	F24I	probably damaging	Het
Vmn2r78	T	C	7: 86,570,094 (GRCm39)	V204A	probably damaging	Het
Vps13a	T	C	19: 16,659,484 (GRCm39)	D1681G	probably benign	Het
Vwa2	T	C	19: 56,897,414 (GRCm39)	V573A	probably benign	Het
Wfdc2	A	G	2: 164,405,279 (GRCm39)	D44G	probably damaging	Het
Zfp40	T	A	17: 23,397,272 (GRCm39)	N75I	probably damaging	Het

Other mutations in Gpr165

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03144:Gpr165	APN	X	95,760,560 (GRCm39)	missense	probably damaging	1.00
R0492:Gpr165	UTSW	X	95,760,778 (GRCm39)	missense	probably damaging	1.00
R8248:Gpr165	UTSW	X	95,757,623 (GRCm39)	missense	probably benign
R8249:Gpr165	UTSW	X	95,757,623 (GRCm39)	missense	probably benign
R8352:Gpr165	UTSW	X	95,757,623 (GRCm39)	missense	probably benign
R8452:Gpr165	UTSW	X	95,757,623 (GRCm39)	missense	probably benign
R8476:Gpr165	UTSW	X	95,757,623 (GRCm39)	missense	probably benign
R8477:Gpr165	UTSW	X	95,757,623 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGTCCACGGCCTTAACTTGC -3'
(R):5'- TGCTCATCCTGGGATACAATTTC -3'

Sequencing Primer
(F):5'- TAAGAGGCTCCTGAGTGA -3'
(R):5'- GTCCCCCGAGTTTGACATGTG -3'

Posted On

2020-10-20