Mutagenetix > Incidental Mutation

Incidental Mutation 'R8511:Hectd1'

655892

Institutional Source

Beutler Lab

Gene Symbol

Hectd1

Ensembl Gene

ENSMUSG00000035247

Gene Name

HECT domain E3 ubiquitin protein ligase 1

Synonyms

A630086P08Rik, b2b327Clo, opm

MMRRC Submission

067888-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8511 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

51790505-51876319 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 51834654 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 870 (S870P)

Ref Sequence

ENSEMBL: ENSMUSP00000136449 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042052] [ENSMUST00000179265]

AlphaFold

Q69ZR2

Predicted Effect

probably benign
Transcript: ENSMUST00000042052
AA Change: S869P

PolyPhen 2 Score 0.208 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000046766
Gene: ENSMUSG00000035247
AA Change: S869P

Domain	Start	End	E-Value	Type
low complexity region	317	331	N/A	INTRINSIC
ANK	395	424	1.44e-1	SMART
ANK	426	455	2.81e-4	SMART
ANK	459	488	1.55e2	SMART
low complexity region	490	509	N/A	INTRINSIC
low complexity region	630	654	N/A	INTRINSIC
low complexity region	707	723	N/A	INTRINSIC
low complexity region	821	832	N/A	INTRINSIC
Pfam:Sad1_UNC	1107	1240	9.2e-27	PFAM
low complexity region	1259	1271	N/A	INTRINSIC
Pfam:MIB_HERC2	1277	1338	7.6e-27	PFAM
low complexity region	1373	1401	N/A	INTRINSIC
low complexity region	1441	1458	N/A	INTRINSIC
low complexity region	1484	1495	N/A	INTRINSIC
low complexity region	1508	1524	N/A	INTRINSIC
low complexity region	1600	1630	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
low complexity region	1674	1703	N/A	INTRINSIC
low complexity region	1745	1752	N/A	INTRINSIC
PDB:2LC3\|A	1879	1966	4e-57	PDB
low complexity region	2101	2117	N/A	INTRINSIC
HECTc	2143	2610	8.32e-76	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000179265
AA Change: S870P

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000136449
Gene: ENSMUSG00000035247
AA Change: S870P

Domain	Start	End	E-Value	Type
low complexity region	317	331	N/A	INTRINSIC
ANK	396	425	1.44e-1	SMART
ANK	427	456	2.81e-4	SMART
ANK	460	489	1.55e2	SMART
low complexity region	491	510	N/A	INTRINSIC
low complexity region	631	655	N/A	INTRINSIC
low complexity region	708	724	N/A	INTRINSIC
low complexity region	822	833	N/A	INTRINSIC
Pfam:Sad1_UNC	1112	1245	1.3e-26	PFAM
low complexity region	1264	1276	N/A	INTRINSIC
Pfam:MIB_HERC2	1282	1341	5.3e-26	PFAM
low complexity region	1378	1406	N/A	INTRINSIC
low complexity region	1446	1463	N/A	INTRINSIC
low complexity region	1489	1500	N/A	INTRINSIC
low complexity region	1513	1529	N/A	INTRINSIC
low complexity region	1605	1635	N/A	INTRINSIC
low complexity region	1638	1656	N/A	INTRINSIC
low complexity region	1679	1708	N/A	INTRINSIC
low complexity region	1750	1757	N/A	INTRINSIC
PDB:2LC3\|A	1884	1971	3e-57	PDB
low complexity region	2106	2122	N/A	INTRINSIC
HECTc	2148	2618	4.5e-72	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice that are homozygous for either a gene trapped or an ENU-induced allele exhibit exencephaly associated with impaired head mesenchyme development and neural tube closure, and show eye and cranial vault dysplasia. Homozygotes for another ENU-induced allele show congenital cardiovascular defects. [provided by MGI curators]

Allele List at MGI

All alleles(30) : Gene trapped(29) Chemically induced(1)

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930512M02Rik	T	C	11: 11,540,056 (GRCm39)	H186R	unknown	Het
6430548M08Rik	A	G	8: 120,879,301 (GRCm39)	N233S	probably benign	Het
Acan	A	G	7: 78,747,683 (GRCm39)	D818G	possibly damaging	Het
Adam22	G	A	5: 8,184,558 (GRCm39)	T478I	probably damaging	Het
Afg2a	A	G	3: 37,490,897 (GRCm39)	M481V	probably damaging	Het
Agmo	T	A	12: 37,294,396 (GRCm39)	*115R	probably null	Het
Ahnak	A	G	19: 8,989,719 (GRCm39)	K3668E	unknown	Het
Aldh6a1	G	A	12: 84,480,745 (GRCm39)	T430I	possibly damaging	Het
Ankrd11	T	C	8: 123,626,468 (GRCm39)	D17G		Het
Apol10b	T	C	15: 77,469,211 (GRCm39)	E322G	probably benign	Het
Apol10b	C	G	15: 77,469,210 (GRCm39)	E322D	probably benign	Het
Apol8	C	T	15: 77,634,273 (GRCm39)	G101E	probably benign	Het
Arhgef26	A	T	3: 62,336,350 (GRCm39)	M630L	probably damaging	Het
Aspm	T	C	1: 139,385,046 (GRCm39)	L230P	probably damaging	Het
Atg101	T	C	15: 101,188,503 (GRCm39)	S203P	probably damaging	Het
Baz2b	T	A	2: 59,732,158 (GRCm39)	T1996S	probably benign	Het
Brwd1	A	T	16: 95,859,938 (GRCm39)	M350K	probably damaging	Het
Cad	A	G	5: 31,233,165 (GRCm39)	K1869R	probably benign	Het
Caskin1	A	T	17: 24,724,910 (GRCm39)	I1233F	probably benign	Het
Ckap5	T	A	2: 91,445,492 (GRCm39)	L1770I	possibly damaging	Het
Clip1	T	C	5: 123,791,969 (GRCm39)	N67S	possibly damaging	Het
Csmd2	A	G	4: 128,262,692 (GRCm39)	N626S		Het
Cuedc2	C	A	19: 46,319,358 (GRCm39)		probably null	Het
Dmbt1	A	T	7: 130,703,742 (GRCm39)	N1275Y	unknown	Het
Dnm3	A	G	1: 162,113,611 (GRCm39)	V483A	possibly damaging	Het
Dock9	A	T	14: 121,864,801 (GRCm39)	H718Q	probably benign	Het
Dock9	T	C	14: 121,918,847 (GRCm39)	D50G	probably damaging	Het
Egfr	T	G	11: 16,846,949 (GRCm39)	I782S	probably damaging	Het
Elmod1	A	T	9: 53,820,095 (GRCm39)	F298I	probably damaging	Het
Enthd1	T	C	15: 80,358,428 (GRCm39)	Q364R	probably damaging	Het
Fat2	T	A	11: 55,200,063 (GRCm39)	S1004C	probably damaging	Het
Fga	A	T	3: 82,939,064 (GRCm39)	K480*	probably null	Het
Fhod3	A	G	18: 25,265,994 (GRCm39)	T1561A	probably damaging	Het
Foxg1	T	A	12: 49,431,868 (GRCm39)	Y200*	probably null	Het
Foxj2	C	T	6: 122,808,404 (GRCm39)	R115*	probably null	Het
Ggnbp2	C	T	11: 84,728,815 (GRCm39)		probably null	Het
Gpsm2	A	G	3: 108,589,399 (GRCm39)	S580P	probably benign	Het
Hic2	T	A	16: 17,075,874 (GRCm39)	N234K	possibly damaging	Het
Ica1	A	G	6: 8,754,726 (GRCm39)	F15L	probably benign	Het
Immp1l	G	A	2: 105,761,100 (GRCm39)	R3H	probably benign	Het
Iqca1l	T	A	5: 24,750,906 (GRCm39)	H559L	possibly damaging	Het
Jak3	T	A	8: 72,138,194 (GRCm39)	Y882N	probably damaging	Het
Klk1b1	C	T	7: 43,619,767 (GRCm39)	R109C	possibly damaging	Het
Luzp1	A	G	4: 136,268,650 (GRCm39)	D291G	probably damaging	Het
Map3k19	C	T	1: 127,775,155 (GRCm39)	E61K	possibly damaging	Het
Mical3	A	G	6: 121,015,513 (GRCm39)	I175T	possibly damaging	Het
Nectin3	G	A	16: 46,284,363 (GRCm39)	P107L	probably damaging	Het
Or10d5	A	G	9: 39,861,455 (GRCm39)	V204A	probably benign	Het
Pira1	A	G	7: 3,742,347 (GRCm39)	L60P	probably damaging	Het
Polr1a	C	A	6: 71,897,504 (GRCm39)	H207N	probably benign	Het
Prokr2	A	G	2: 132,223,422 (GRCm39)	V40A	probably benign	Het
Prtg	T	C	9: 72,798,156 (GRCm39)		probably null	Het
Ptprs	G	A	17: 56,754,440 (GRCm39)	T200I	probably damaging	Het
Ramp3	C	T	11: 6,626,709 (GRCm39)	R139C	probably benign	Het
Scfd2	A	G	5: 74,372,949 (GRCm39)	V642A	possibly damaging	Het
Scn11a	T	C	9: 119,618,981 (GRCm39)	K787R	probably damaging	Het
Slco3a1	A	G	7: 73,952,990 (GRCm39)	V523A	probably benign	Het
Smo	A	G	6: 29,755,531 (GRCm39)	Y401C	probably damaging	Het
Stmn2	A	G	3: 8,574,615 (GRCm39)		probably benign	Het
Stra6l	G	T	4: 45,885,347 (GRCm39)	G605V	probably benign	Het
Syne2	A	G	12: 76,055,647 (GRCm39)	I4170V	probably benign	Het
Tgm7	T	A	2: 120,924,141 (GRCm39)	I594F	probably damaging	Het
Tle2	T	C	10: 81,423,830 (GRCm39)	L648P	probably damaging	Het
Tmprss2	G	T	16: 97,369,662 (GRCm39)	L371I	possibly damaging	Het
Ttn	T	C	2: 76,579,866 (GRCm39)	R23676G	probably damaging	Het
Tvp23b	T	C	11: 62,774,563 (GRCm39)	I69T	possibly damaging	Het
Vmn2r66	A	T	7: 84,656,026 (GRCm39)	I330N	probably damaging	Het
Vmn2r99	A	T	17: 19,614,443 (GRCm39)	D721V	probably damaging	Het
Zan	A	T	5: 137,445,108 (GRCm39)	V1717E	unknown	Het
Zmiz2	C	T	11: 6,353,190 (GRCm39)	H658Y	probably damaging	Het

Other mutations in Hectd1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Hectd1	APN	12	51,806,215 (GRCm39)	missense	probably benign
IGL00402:Hectd1	APN	12	51,815,891 (GRCm39)	missense	possibly damaging	0.94
IGL00419:Hectd1	APN	12	51,810,818 (GRCm39)	missense	probably damaging	0.99
IGL00518:Hectd1	APN	12	51,823,272 (GRCm39)	splice site	probably benign
IGL00565:Hectd1	APN	12	51,837,181 (GRCm39)	missense	probably damaging	0.97
IGL00574:Hectd1	APN	12	51,820,787 (GRCm39)	missense	probably benign	0.17
IGL00576:Hectd1	APN	12	51,806,092 (GRCm39)	missense	probably damaging	0.99
IGL00788:Hectd1	APN	12	51,795,571 (GRCm39)	missense	probably damaging	0.99
IGL00978:Hectd1	APN	12	51,838,173 (GRCm39)	missense	possibly damaging	0.95
IGL01328:Hectd1	APN	12	51,807,904 (GRCm39)	missense	probably damaging	1.00
IGL01337:Hectd1	APN	12	51,849,057 (GRCm39)	missense	possibly damaging	0.95
IGL01634:Hectd1	APN	12	51,850,562 (GRCm39)	missense	probably damaging	0.98
IGL01731:Hectd1	APN	12	51,849,593 (GRCm39)	missense	possibly damaging	0.59
IGL01920:Hectd1	APN	12	51,829,337 (GRCm39)	missense	probably damaging	0.99
IGL01951:Hectd1	APN	12	51,841,280 (GRCm39)	nonsense	probably null
IGL01994:Hectd1	APN	12	51,844,725 (GRCm39)	missense	probably damaging	0.99
IGL02140:Hectd1	APN	12	51,820,920 (GRCm39)	missense	probably damaging	0.99
IGL02150:Hectd1	APN	12	51,815,974 (GRCm39)	missense	probably damaging	0.97
IGL02156:Hectd1	APN	12	51,800,916 (GRCm39)	splice site	probably benign
IGL02177:Hectd1	APN	12	51,819,103 (GRCm39)	missense	probably damaging	0.99
IGL02502:Hectd1	APN	12	51,844,635 (GRCm39)	missense	possibly damaging	0.77
IGL02505:Hectd1	APN	12	51,847,496 (GRCm39)	critical splice donor site	probably null
IGL02519:Hectd1	APN	12	51,815,894 (GRCm39)	missense	probably damaging	0.99
IGL02624:Hectd1	APN	12	51,809,233 (GRCm39)	missense	possibly damaging	0.61
IGL02833:Hectd1	APN	12	51,810,864 (GRCm39)	missense	probably damaging	0.96
IGL02851:Hectd1	APN	12	51,814,423 (GRCm39)	missense	possibly damaging	0.94
IGL02866:Hectd1	APN	12	51,837,396 (GRCm39)	missense	probably damaging	1.00
IGL02981:Hectd1	APN	12	51,815,670 (GRCm39)	missense	possibly damaging	0.70
IGL02987:Hectd1	APN	12	51,791,550 (GRCm39)	missense	probably damaging	1.00
IGL02999:Hectd1	APN	12	51,874,205 (GRCm39)	missense	possibly damaging	0.77
IGL03071:Hectd1	APN	12	51,815,957 (GRCm39)	missense	probably benign	0.00
IGL03078:Hectd1	APN	12	51,849,019 (GRCm39)	missense	probably damaging	0.98
IGL03299:Hectd1	APN	12	51,847,671 (GRCm39)	splice site	probably benign
3-1:Hectd1	UTSW	12	51,800,590 (GRCm39)	missense	probably damaging	0.99
R0039:Hectd1	UTSW	12	51,800,608 (GRCm39)	missense	possibly damaging	0.83
R0238:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0238:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0239:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0239:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0268:Hectd1	UTSW	12	51,815,891 (GRCm39)	missense	possibly damaging	0.94
R0268:Hectd1	UTSW	12	51,815,890 (GRCm39)	missense	probably damaging	0.99
R0409:Hectd1	UTSW	12	51,829,339 (GRCm39)	missense	possibly damaging	0.59
R1019:Hectd1	UTSW	12	51,795,440 (GRCm39)	missense	probably damaging	0.99
R1072:Hectd1	UTSW	12	51,807,855 (GRCm39)	missense	probably benign	0.11
R1087:Hectd1	UTSW	12	51,823,355 (GRCm39)	missense	probably damaging	0.99
R1165:Hectd1	UTSW	12	51,810,947 (GRCm39)	splice site	probably benign
R1350:Hectd1	UTSW	12	51,809,217 (GRCm39)	missense	probably benign
R1553:Hectd1	UTSW	12	51,820,661 (GRCm39)	missense	probably damaging	0.98
R1666:Hectd1	UTSW	12	51,800,607 (GRCm39)	missense	possibly damaging	0.91
R1676:Hectd1	UTSW	12	51,791,571 (GRCm39)	missense	probably damaging	1.00
R1694:Hectd1	UTSW	12	51,791,375 (GRCm39)	missense	probably damaging	1.00
R1778:Hectd1	UTSW	12	51,800,590 (GRCm39)	missense	probably damaging	0.99
R1856:Hectd1	UTSW	12	51,791,577 (GRCm39)	missense	probably damaging	1.00
R1859:Hectd1	UTSW	12	51,853,350 (GRCm39)	missense	probably damaging	1.00
R1884:Hectd1	UTSW	12	51,847,738 (GRCm39)	missense	probably benign	0.00
R1982:Hectd1	UTSW	12	51,832,624 (GRCm39)	missense	probably damaging	0.97
R2034:Hectd1	UTSW	12	51,803,899 (GRCm39)	splice site	probably null
R2061:Hectd1	UTSW	12	51,841,227 (GRCm39)	missense	probably damaging	0.99
R2078:Hectd1	UTSW	12	51,795,325 (GRCm39)	missense	probably damaging	0.99
R2176:Hectd1	UTSW	12	51,792,277 (GRCm39)	missense	probably damaging	1.00
R2210:Hectd1	UTSW	12	51,853,245 (GRCm39)	missense	probably damaging	0.99
R2248:Hectd1	UTSW	12	51,853,254 (GRCm39)	missense	probably damaging	0.99
R2282:Hectd1	UTSW	12	51,815,791 (GRCm39)	missense	possibly damaging	0.95
R2402:Hectd1	UTSW	12	51,792,317 (GRCm39)	missense	probably benign	0.01
R3876:Hectd1	UTSW	12	51,815,513 (GRCm39)	missense	probably damaging	0.98
R4027:Hectd1	UTSW	12	51,849,219 (GRCm39)	critical splice acceptor site	probably null
R4085:Hectd1	UTSW	12	51,821,533 (GRCm39)	missense	possibly damaging	0.93
R4115:Hectd1	UTSW	12	51,815,506 (GRCm39)	nonsense	probably null
R4116:Hectd1	UTSW	12	51,815,506 (GRCm39)	nonsense	probably null
R4169:Hectd1	UTSW	12	51,837,008 (GRCm39)	missense	probably damaging	0.97
R4434:Hectd1	UTSW	12	51,798,835 (GRCm39)	missense	probably damaging	1.00
R4507:Hectd1	UTSW	12	51,837,276 (GRCm39)	missense	probably damaging	0.97
R4578:Hectd1	UTSW	12	51,798,715 (GRCm39)	missense	probably damaging	1.00
R4579:Hectd1	UTSW	12	51,791,356 (GRCm39)	missense	probably damaging	0.97
R4709:Hectd1	UTSW	12	51,834,695 (GRCm39)	missense	possibly damaging	0.94
R4812:Hectd1	UTSW	12	51,874,134 (GRCm39)	critical splice donor site	probably null
R4883:Hectd1	UTSW	12	51,831,030 (GRCm39)	nonsense	probably null
R4885:Hectd1	UTSW	12	51,847,505 (GRCm39)	missense	probably damaging	0.97
R4975:Hectd1	UTSW	12	51,809,280 (GRCm39)	missense	probably benign	0.02
R4983:Hectd1	UTSW	12	51,831,045 (GRCm39)	missense	probably benign	0.01
R5007:Hectd1	UTSW	12	51,849,443 (GRCm39)	missense	possibly damaging	0.95
R5046:Hectd1	UTSW	12	51,797,171 (GRCm39)	missense	probably damaging	1.00
R5062:Hectd1	UTSW	12	51,791,662 (GRCm39)	missense	probably damaging	0.98
R5164:Hectd1	UTSW	12	51,874,272 (GRCm39)	start codon destroyed	probably null	0.60
R5213:Hectd1	UTSW	12	51,849,316 (GRCm39)	critical splice donor site	probably null
R5535:Hectd1	UTSW	12	51,849,109 (GRCm39)	missense	probably damaging	0.98
R5776:Hectd1	UTSW	12	51,810,897 (GRCm39)	missense	possibly damaging	0.91
R5846:Hectd1	UTSW	12	51,820,618 (GRCm39)	missense	probably damaging	0.99
R5907:Hectd1	UTSW	12	51,845,537 (GRCm39)	missense	probably damaging	0.98
R5911:Hectd1	UTSW	12	51,849,035 (GRCm39)	missense	probably damaging	0.99
R5919:Hectd1	UTSW	12	51,815,855 (GRCm39)	missense	probably damaging	0.98
R6051:Hectd1	UTSW	12	51,800,887 (GRCm39)	missense	probably benign
R6141:Hectd1	UTSW	12	51,792,875 (GRCm39)	critical splice donor site	probably null
R6172:Hectd1	UTSW	12	51,816,065 (GRCm39)	missense	probably damaging	1.00
R6194:Hectd1	UTSW	12	51,795,228 (GRCm39)	missense	probably damaging	0.99
R6356:Hectd1	UTSW	12	51,791,402 (GRCm39)	missense	probably damaging	1.00
R6795:Hectd1	UTSW	12	51,841,270 (GRCm39)	missense	possibly damaging	0.94
R6909:Hectd1	UTSW	12	51,810,945 (GRCm39)	splice site	probably null
R6971:Hectd1	UTSW	12	51,795,526 (GRCm39)	nonsense	probably null
R7079:Hectd1	UTSW	12	51,834,638 (GRCm39)	missense	possibly damaging	0.96
R7104:Hectd1	UTSW	12	51,874,134 (GRCm39)	critical splice donor site	probably null
R7171:Hectd1	UTSW	12	51,806,080 (GRCm39)	missense	probably damaging	0.99
R7296:Hectd1	UTSW	12	51,832,635 (GRCm39)	missense	possibly damaging	0.73
R7346:Hectd1	UTSW	12	51,797,104 (GRCm39)	missense	probably benign
R7355:Hectd1	UTSW	12	51,838,081 (GRCm39)	missense	possibly damaging	0.72
R7468:Hectd1	UTSW	12	51,791,588 (GRCm39)	splice site	probably null
R7531:Hectd1	UTSW	12	51,853,150 (GRCm39)	missense	probably benign	0.33
R7532:Hectd1	UTSW	12	51,837,233 (GRCm39)	missense	probably damaging	0.98
R7755:Hectd1	UTSW	12	51,849,003 (GRCm39)	missense	possibly damaging	0.86
R7807:Hectd1	UTSW	12	51,792,171 (GRCm39)	missense	probably damaging	1.00
R7842:Hectd1	UTSW	12	51,819,343 (GRCm39)	missense	probably damaging	0.99
R7922:Hectd1	UTSW	12	51,836,978 (GRCm39)	nonsense	probably null
R8059:Hectd1	UTSW	12	51,837,161 (GRCm39)	missense	possibly damaging	0.53
R8085:Hectd1	UTSW	12	51,795,679 (GRCm39)	missense	probably damaging	0.97
R8145:Hectd1	UTSW	12	51,831,016 (GRCm39)	missense	possibly damaging	0.72
R8157:Hectd1	UTSW	12	51,838,073 (GRCm39)	missense	possibly damaging	0.53
R8405:Hectd1	UTSW	12	51,874,178 (GRCm39)	missense	probably benign	0.01
R8505:Hectd1	UTSW	12	51,797,145 (GRCm39)	missense	probably damaging	1.00
R8697:Hectd1	UTSW	12	51,819,320 (GRCm39)	critical splice donor site	probably benign
R8725:Hectd1	UTSW	12	51,849,000 (GRCm39)	missense	possibly damaging	0.92
R8727:Hectd1	UTSW	12	51,849,000 (GRCm39)	missense	possibly damaging	0.92
R8911:Hectd1	UTSW	12	51,795,616 (GRCm39)	missense	probably damaging	0.99
R8983:Hectd1	UTSW	12	51,791,410 (GRCm39)	missense	probably damaging	0.97
R9037:Hectd1	UTSW	12	51,832,665 (GRCm39)	missense	possibly damaging	0.85
R9219:Hectd1	UTSW	12	51,800,612 (GRCm39)	missense	probably damaging	0.99
R9413:Hectd1	UTSW	12	51,792,880 (GRCm39)	nonsense	probably null
R9456:Hectd1	UTSW	12	51,832,584 (GRCm39)	missense	probably benign
R9513:Hectd1	UTSW	12	51,816,079 (GRCm39)	missense	possibly damaging	0.92
R9640:Hectd1	UTSW	12	51,795,197 (GRCm39)	nonsense	probably null
R9641:Hectd1	UTSW	12	51,816,047 (GRCm39)	missense	probably benign	0.00
R9713:Hectd1	UTSW	12	51,823,328 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- ACCTTGACTGTAGAAAAGAAAGCC -3'
(R):5'- GCTCAGGAGTGGATAAGCTG -3'

Sequencing Primer
(F):5'- GTGGTTCACAGAAACAGCTATCTG -3'
(R):5'- CTCAGGAGTGGATAAGCTGTTAATC -3'

Posted On

2020-10-20