Incidental Mutation 'R8516:Zfp953'
ID 656168
Institutional Source Beutler Lab
Gene Symbol Zfp953
Ensembl Gene ENSMUSG00000098905
Gene Name zinc finger protein 953
Synonyms E130120F12Rik
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.061) question?
Stock # R8516 (G1)
Quality Score 225.009
Status Not validated
Chromosome 13
Chromosomal Location 67339306-67360605 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 67345355 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Phenylalanine at position 75 (Y75F)
Ref Sequence ENSEMBL: ENSMUSP00000076700 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000044819] [ENSMUST00000081582] [ENSMUST00000166080]
AlphaFold no structure available at present
Predicted Effect probably benign
Transcript: ENSMUST00000044819
AA Change: Y75F

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)
SMART Domains Protein: ENSMUSP00000049225
Gene: ENSMUSG00000098781
AA Change: Y75F

DomainStartEndE-ValueType
KRAB 5 65 1.15e-23 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000081582
AA Change: Y75F

PolyPhen 2 Score 0.549 (Sensitivity: 0.88; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000076700
Gene: ENSMUSG00000098905
AA Change: Y75F

DomainStartEndE-ValueType
KRAB 5 65 1.15e-23 SMART
ZnF_C2H2 81 103 3.11e-2 SMART
ZnF_C2H2 109 131 8.94e-3 SMART
ZnF_C2H2 137 159 6.23e-2 SMART
ZnF_C2H2 165 187 3.63e-3 SMART
ZnF_C2H2 193 215 1.06e-4 SMART
ZnF_C2H2 221 243 7.05e-1 SMART
ZnF_C2H2 249 271 5.42e-2 SMART
ZnF_C2H2 277 299 6.88e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000166080
SMART Domains Protein: ENSMUSP00000126669
Gene: ENSMUSG00000098692

DomainStartEndE-ValueType
KRAB 2 62 3.07e-33 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.5%
  • 20x: 98.6%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aasdhppt A T 9: 4,309,373 S22T probably benign Het
Actr8 G T 14: 29,990,899 A500S probably benign Het
Adamtsl1 A G 4: 86,342,543 Y1005C probably damaging Het
Ank3 T A 10: 69,927,729 Y884* probably null Het
Arhgap28 T C 17: 67,873,073 R306G probably benign Het
Atp8a2 A C 14: 59,691,472 I1044M probably benign Het
Bahd1 A G 2: 118,916,971 Y357C probably benign Het
Btd A T 14: 31,666,867 T182S probably damaging Het
Casc3 C T 11: 98,822,781 R280C probably damaging Het
Cisd2 T C 3: 135,411,013 T106A probably damaging Het
Cldn15 G T 5: 136,974,696 C184F probably damaging Het
Clk4 G A 11: 51,275,261 R198Q probably damaging Het
Coprs G T 8: 13,885,065 F163L probably damaging Het
Csmd3 G A 15: 47,629,365 R2216* probably null Het
Defb7 A T 8: 19,497,607 I43F possibly damaging Het
Dpp8 C A 9: 65,078,009 T783K probably damaging Het
Eif2s1 G A 12: 78,881,162 G204D probably damaging Het
Elavl4 T C 4: 110,251,379 N56S probably damaging Het
Emilin1 G A 5: 30,917,171 R252H probably damaging Het
Exd1 A T 2: 119,520,073 L569Q probably damaging Het
Gm498 T A 7: 143,897,274 I342N probably damaging Het
Gpn2 C T 4: 133,584,831 R125C probably damaging Het
Gria2 T C 3: 80,706,987 E582G probably benign Het
Hadha A T 5: 30,126,584 V458E probably damaging Het
Hap1 T C 11: 100,356,067 K4R possibly damaging Het
Hectd4 A T 5: 121,349,010 H3356L possibly damaging Het
Herc2 G A 7: 56,206,570 V3919I probably benign Het
Lgr6 G T 1: 135,075,283 N76K probably damaging Het
Olfr692 A G 7: 105,368,769 I148V probably benign Het
P4ha3 A G 7: 100,314,662 M462V probably damaging Het
Pde3b A T 7: 114,526,849 M773L probably benign Het
Peak1 T C 9: 56,260,000 S215G probably damaging Het
Pgm5 T A 19: 24,815,710 M331L probably benign Het
Piwil2 A G 14: 70,420,739 V213A probably benign Het
Plch2 T C 4: 154,986,307 H1205R probably benign Het
Pop4 A T 7: 38,267,402 M85K probably benign Het
Ppp3ca T A 3: 136,877,768 I212N probably damaging Het
Prom1 T C 5: 44,007,099 K714R probably benign Het
Psip1 C T 4: 83,466,715 G207S probably benign Het
Rgs22 A G 15: 36,010,335 *1259Q probably null Het
Scn1a C T 2: 66,326,134 G477D possibly damaging Het
Sf3b1 T C 1: 55,012,103 E222G probably null Het
Snrpa1 G A 7: 66,070,633 G195R probably benign Het
Spem2 T C 11: 69,816,895 R415G possibly damaging Het
Tmem167 T A 13: 90,098,396 V13E probably damaging Het
Trim2 C T 3: 84,208,320 A102T probably damaging Het
Trim30b A G 7: 104,357,404 S82P probably benign Het
Uba6 A C 5: 86,127,748 S760R possibly damaging Het
Upf2 T C 2: 6,018,971 F711L unknown Het
Utrn T A 10: 12,486,510 D2693V probably damaging Het
Vmn1r30 T G 6: 58,435,124 Y241S probably damaging Het
Vmn2r110 A G 17: 20,574,613 L598P probably damaging Het
Wfdc18 T A 11: 83,709,158 F14Y probably benign Het
Wnt10b A T 15: 98,772,880 C256S probably damaging Het
Xrn1 T A 9: 96,048,391 Y1554* probably null Het
Zc3h12a T A 4: 125,119,839 S411C probably damaging Het
Zfp112 G A 7: 24,123,964 G63E probably benign Het
Zfp786 A T 6: 47,820,543 L487Q probably damaging Het
Other mutations in Zfp953
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03034:Zfp953 APN 13 67343462 missense probably damaging 1.00
IGL03347:Zfp953 APN 13 67343426 missense probably benign 0.05
R0111:Zfp953 UTSW 13 67343075 missense probably damaging 1.00
R1856:Zfp953 UTSW 13 67345358 missense probably benign 0.02
R2518:Zfp953 UTSW 13 67347939 missense probably damaging 0.99
R3925:Zfp953 UTSW 13 67347938 missense probably damaging 0.99
R4777:Zfp953 UTSW 13 67343129 missense probably benign 0.42
R5647:Zfp953 UTSW 13 67343472 missense possibly damaging 0.90
R6232:Zfp953 UTSW 13 67343097 missense possibly damaging 0.94
R6481:Zfp953 UTSW 13 67347937 nonsense probably null
R7202:Zfp953 UTSW 13 67343642 missense probably benign 0.02
R7543:Zfp953 UTSW 13 67347889 missense probably damaging 1.00
R8155:Zfp953 UTSW 13 67343471 missense probably damaging 0.99
R9317:Zfp953 UTSW 13 67343393 missense possibly damaging 0.79
Z1177:Zfp953 UTSW 13 67343003 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AACGTTAGCTTATGCCCTCC -3'
(R):5'- TCCTTATACACACAGTCTTGGAG -3'

Sequencing Primer
(F):5'- GGCTAACTGAAAACCTGTTGCCTG -3'
(R):5'- GAGGGACATTTTATTTCCCATGTGAC -3'
Posted On 2020-10-20