Mutagenetix > Incidental Mutation

Incidental Mutation 'R8017:Zfp873'

656306

Institutional Source

Beutler Lab

Gene Symbol

Zfp873

Ensembl Gene

ENSMUSG00000061371

Gene Name

zinc finger protein 873

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R8017 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

82048123-82064745 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 82060359 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 308 (V308E)

Ref Sequence

ENSEMBL: ENSMUSP00000100950 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105313] [ENSMUST00000209622] [ENSMUST00000210325]

AlphaFold

A0A1B0GT64

Predicted Effect

probably benign
Transcript: ENSMUST00000105313
AA Change: V308E

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000100950
Gene: ENSMUSG00000061371
AA Change: V308E

Domain	Start	End	E-Value	Type
ZnF_C2H2	68	90	1.12e2	SMART
ZnF_C2H2	96	117	4.69e0	SMART
ZnF_C2H2	123	145	2.06e1	SMART
ZnF_C2H2	151	173	5.5e-3	SMART
ZnF_C2H2	179	201	3.69e-4	SMART
ZnF_C2H2	207	229	3.89e-3	SMART
ZnF_C2H2	235	257	9.88e-5	SMART
ZnF_C2H2	263	285	5.59e-4	SMART
ZnF_C2H2	291	313	2.99e-4	SMART
ZnF_C2H2	319	341	1.95e-3	SMART
ZnF_C2H2	347	369	2.75e-3	SMART
ZnF_C2H2	375	397	7.37e-4	SMART
ZnF_C2H2	403	425	2.53e-2	SMART
ZnF_C2H2	431	453	9.08e-4	SMART
ZnF_C2H2	459	481	5.99e-4	SMART
ZnF_C2H2	487	509	1.22e-4	SMART
ZnF_C2H2	515	537	1.82e-3	SMART
ZnF_C2H2	543	565	2.53e-2	SMART
ZnF_C2H2	571	593	9.73e-4	SMART
ZnF_C2H2	599	619	3.13e1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000209622

Predicted Effect

probably benign
Transcript: ENSMUST00000210325
AA Change: V345E

PolyPhen 2 Score 0.028 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

95% (36/38)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aar2	T	G	2: 156,555,956	I296S	probably benign	Het
Acsl5	A	G	19: 55,268,796	I42V	probably benign	Het
Adam25	T	A	8: 40,754,087	M130K	possibly damaging	Het
Bcl2l2	T	A	14: 54,884,383	M1K	probably null	Het
Casc1	A	G	6: 145,194,557	S173P	probably damaging	Het
Cdh16	T	C	8: 104,616,267	N724S	probably damaging	Het
Cfi	T	C	3: 129,855,099	S211P	probably benign	Het
Clpsl2	G	A	17: 28,550,728	G55R	probably damaging	Het
Crybg2	T	A	4: 134,073,173	V239E	possibly damaging	Het
Dcn	G	A	10: 97,483,535	R58Q	probably damaging	Het
Dnah10	A	G	5: 124,800,885	N2678S	probably benign	Het
Fam205a1	T	C	4: 42,850,840	T439A	probably damaging	Het
Fam214b	A	T	4: 43,034,413	F394Y	probably damaging	Het
Fbxo24	C	A	5: 137,612,811	M572I	probably benign	Het
Gm13212	A	G	4: 145,622,568	T192A	probably benign	Het
Hnrnpul1	T	C	7: 25,748,464	E145G	probably benign	Het
Ica1	A	G	6: 8,658,286	V277A	probably benign	Het
Idi1	G	A	13: 8,887,938	S140N	probably benign	Het
Kank1	TGCGA	T	19: 25,411,204		probably null	Het
Kank1	GCGAACG	GCG	19: 25,411,205		probably null	Het
Kif20b	T	C	19: 34,939,879	V603A	probably damaging	Het
Klc2	T	C	19: 5,111,839	K276R	probably benign	Het
Lmbrd2	T	A	15: 9,172,230	N370K	probably benign	Het
Map2k5	G	T	9: 63,339,121	A108D	probably damaging	Het
Obox2	T	C	7: 15,397,049	S69P	possibly damaging	Het
Olfr1308	G	A	2: 111,960,573	P167S	probably damaging	Het
Olfr1364	G	A	13: 21,574,478		probably benign	Het
Olfr32	A	C	2: 90,138,826	F104L	probably benign	Het
Pcdhga8	A	G	18: 37,727,730	E613G	probably damaging	Het
Pira2	A	T	7: 3,841,697	F445Y	probably benign	Het
Rab2a	T	C	4: 8,604,444		probably null	Het
Rsph4a	A	C	10: 33,909,459	R455S	probably damaging	Het
St6gal1	G	A	16: 23,357,835	A393T	probably benign	Het
Tmem151a	T	C	19: 5,082,560	E206G	probably damaging	Het
Tmem156	C	T	5: 65,073,861	C222Y	probably damaging	Het
Tnfrsf11b	C	T	15: 54,254,202	W219*	probably null	Het
Ttc36	T	C	9: 44,799,601	E144G	probably damaging	Het
Ttn	C	T	2: 76,767,457	R19704H	probably damaging	Het
Wdr17	A	C	8: 54,638,368	I1137S	possibly damaging	Het
Xxylt1	A	G	16: 31,007,819	L226P	probably damaging	Het
Zbtb8b	G	A	4: 129,428,445	R408W	probably damaging	Het

Other mutations in Zfp873

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02250:Zfp873	APN	10	82058418	start codon destroyed	probably null	0.45
G1Funyon:Zfp873	UTSW	10	82060879	missense	probably damaging	1.00
R0666:Zfp873	UTSW	10	82060761	missense	possibly damaging	0.75
R1568:Zfp873	UTSW	10	82060279	missense	probably damaging	1.00
R1739:Zfp873	UTSW	10	82060707	missense	probably damaging	1.00
R1848:Zfp873	UTSW	10	82060572	missense	probably benign	0.33
R1892:Zfp873	UTSW	10	82061246	missense	probably damaging	1.00
R2061:Zfp873	UTSW	10	82060157	missense	probably benign	0.01
R3735:Zfp873	UTSW	10	82061181	missense	probably benign	0.15
R4422:Zfp873	UTSW	10	82060874	missense	probably benign	0.13
R4674:Zfp873	UTSW	10	82059980	missense	possibly damaging	0.53
R4839:Zfp873	UTSW	10	82060519	missense	probably damaging	0.98
R5146:Zfp873	UTSW	10	82060224	missense	probably damaging	1.00
R5154:Zfp873	UTSW	10	82060191	missense	possibly damaging	0.54
R5160:Zfp873	UTSW	10	82061042	missense	possibly damaging	0.54
R5811:Zfp873	UTSW	10	82060733	missense	probably damaging	1.00
R6625:Zfp873	UTSW	10	82060304	missense	probably damaging	1.00
R6667:Zfp873	UTSW	10	82060589	missense	probably benign	0.22
R6742:Zfp873	UTSW	10	82058422	missense	probably damaging	1.00
R6878:Zfp873	UTSW	10	82060695	missense	probably benign	0.33
R7055:Zfp873	UTSW	10	82059998	missense	probably damaging	1.00
R7296:Zfp873	UTSW	10	82061237	missense	probably damaging	1.00
R7381:Zfp873	UTSW	10	82060971	missense	probably damaging	1.00
R7448:Zfp873	UTSW	10	82060627	missense	probably damaging	1.00
R7464:Zfp873	UTSW	10	82060376	missense	possibly damaging	0.51
R7470:Zfp873	UTSW	10	82059939	missense	probably benign	0.19
R7640:Zfp873	UTSW	10	82060275	missense	possibly damaging	0.72
R7862:Zfp873	UTSW	10	82060275	missense	probably benign	0.03
R8301:Zfp873	UTSW	10	82060879	missense	probably damaging	1.00
R8765:Zfp873	UTSW	10	82060238	missense	probably damaging	1.00
R8997:Zfp873	UTSW	10	82061156	missense	probably benign	0.03
R9305:Zfp873	UTSW	10	82060680	missense	probably benign	0.00
R9462:Zfp873	UTSW	10	82061297	missense	probably benign	0.31
Z1177:Zfp873	UTSW	10	82061165	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CATACTGGAGAGAAACCGTATGA -3'
(R):5'- CTGTCCCGTGTGAGTTCTT -3'

Sequencing Primer
(F):5'- TGTAGTAAAGCCTTTCCAAGTCCCAG -3'
(R):5'- GTGTACCTGCAGATTACTGAGCC -3'

Posted On

2020-10-20