Incidental Mutation 'R8466:Pla2g4f'
ID 656812
Institutional Source Beutler Lab
Gene Symbol Pla2g4f
Ensembl Gene ENSMUSG00000046971
Gene Name phospholipase A2, group IVF
Synonyms 4732472I07Rik, Pla2zeta
MMRRC Submission 067910-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.060) question?
Stock # R8466 (G1)
Quality Score 225.009
Status Not validated
Chromosome 2
Chromosomal Location 120130438-120144646 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 120130963 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Aspartic acid at position 831 (N831D)
Ref Sequence ENSEMBL: ENSMUSP00000062607 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000054651]
AlphaFold Q50L41
Predicted Effect probably damaging
Transcript: ENSMUST00000054651
AA Change: N831D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000062607
Gene: ENSMUSG00000046971
AA Change: N831D

DomainStartEndE-ValueType
C2 45 144 7.51e-11 SMART
PLAc 285 797 1.6e-13 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.9%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 50 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700017N19Rik A G 10: 100,437,873 (GRCm39) E94G probably benign Het
Abhd16a G T 17: 35,313,236 (GRCm39) R118L probably damaging Het
Adamts1 A G 16: 85,599,400 (GRCm39) S67P probably benign Het
Adar C T 3: 89,658,466 (GRCm39) P656L probably damaging Het
Afg3l1 G A 8: 124,216,648 (GRCm39) D296N probably benign Het
Akap9 G A 5: 4,088,659 (GRCm39) R2096Q probably damaging Het
Aldh1a2 T A 9: 71,160,205 (GRCm39) I77K probably benign Het
Cacnb4 A C 2: 52,354,679 (GRCm39) V233G probably damaging Het
Cage1 T A 13: 38,206,987 (GRCm39) Q286L probably damaging Het
Camta1 T A 4: 151,170,577 (GRCm39) K1055* probably null Het
Ccdc96 C A 5: 36,642,252 (GRCm39) probably benign Het
Cep70 T C 9: 99,160,073 (GRCm39) probably null Het
Cwc27 G T 13: 104,940,772 (GRCm39) P196T probably benign Het
Cwc27 C A 13: 104,940,776 (GRCm39) L194F possibly damaging Het
Ddx24 G T 12: 103,376,160 (GRCm39) L779I probably benign Het
Dock7 T C 4: 98,952,336 (GRCm39) E378G possibly damaging Het
Fan1 T A 7: 64,022,234 (GRCm39) N340Y probably damaging Het
Flnc A G 6: 29,438,621 (GRCm39) N172D probably damaging Het
Gprin3 C A 6: 59,331,466 (GRCm39) Q280H possibly damaging Het
Gprin3 T A 6: 59,331,467 (GRCm39) Q280L probably benign Het
Gucy2d C A 7: 98,099,237 (GRCm39) P351Q probably damaging Het
H2-M11 G A 17: 36,858,985 (GRCm39) G175D probably benign Het
Klhl42 C T 6: 147,009,241 (GRCm39) T360M probably benign Het
Lama1 G A 17: 68,120,948 (GRCm39) E2695K Het
Lsg1 T C 16: 30,400,919 (GRCm39) Q130R probably benign Het
Macf1 T C 4: 123,349,237 (GRCm39) T2100A probably benign Het
Muc16 T C 9: 18,554,444 (GRCm39) T3950A unknown Het
Myo1c G A 11: 75,549,213 (GRCm39) R109H probably damaging Het
Nol4 C A 18: 23,171,638 (GRCm39) A8S probably benign Het
Or1e1f A T 11: 73,855,913 (GRCm39) T160S probably damaging Het
Or1p1b A G 11: 74,131,016 (GRCm39) I209V probably benign Het
Pabpn1l C T 8: 123,347,625 (GRCm39) V216M possibly damaging Het
Papln A C 12: 83,825,255 (GRCm39) probably null Het
Pcsk5 A T 19: 17,549,864 (GRCm39) C709* probably null Het
Pglyrp3 T A 3: 91,921,941 (GRCm39) V3E probably benign Het
Prp2rt T C 13: 97,235,492 (GRCm39) D85G probably damaging Het
Prss22 T C 17: 24,215,802 (GRCm39) D40G probably benign Het
Rap1gap2 A G 11: 74,316,057 (GRCm39) F208S probably benign Het
Sipa1l2 A T 8: 126,218,985 (GRCm39) N117K probably damaging Het
Srsf9 G C 5: 115,465,492 (GRCm39) R42P probably benign Het
Stat3 A T 11: 100,785,924 (GRCm39) I451N probably damaging Het
Tecrl A T 5: 83,428,367 (GRCm39) Y301* probably null Het
Togaram1 T C 12: 65,033,216 (GRCm39) S1065P probably benign Het
Usp48 T A 4: 137,350,630 (GRCm39) L39Q probably null Het
Utp20 A T 10: 88,654,365 (GRCm39) S241T probably damaging Het
Vmn2r50 A G 7: 9,783,997 (GRCm39) F159S probably damaging Het
Wdr76 A C 2: 121,341,038 (GRCm39) N28H probably damaging Het
Zfhx2 T A 14: 55,310,353 (GRCm39) Y731F possibly damaging Het
Zfhx4 A T 3: 5,307,762 (GRCm39) E329D probably damaging Het
Zswim8 A T 14: 20,760,744 (GRCm39) Q83L possibly damaging Het
Other mutations in Pla2g4f
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00539:Pla2g4f APN 2 120,133,219 (GRCm39) missense possibly damaging 0.53
IGL01652:Pla2g4f APN 2 120,132,716 (GRCm39) missense possibly damaging 0.86
IGL02792:Pla2g4f APN 2 120,133,850 (GRCm39) missense probably damaging 1.00
R0625:Pla2g4f UTSW 2 120,135,522 (GRCm39) missense probably damaging 1.00
R1760:Pla2g4f UTSW 2 120,144,547 (GRCm39) unclassified probably benign
R1799:Pla2g4f UTSW 2 120,141,549 (GRCm39) missense possibly damaging 0.49
R2212:Pla2g4f UTSW 2 120,133,587 (GRCm39) missense probably benign
R2351:Pla2g4f UTSW 2 120,130,923 (GRCm39) missense probably benign 0.01
R3412:Pla2g4f UTSW 2 120,133,587 (GRCm39) missense probably benign
R3414:Pla2g4f UTSW 2 120,133,587 (GRCm39) missense probably benign
R3906:Pla2g4f UTSW 2 120,130,980 (GRCm39) missense probably benign 0.28
R4084:Pla2g4f UTSW 2 120,142,806 (GRCm39) missense probably benign 0.36
R4477:Pla2g4f UTSW 2 120,134,153 (GRCm39) missense probably damaging 1.00
R4529:Pla2g4f UTSW 2 120,131,100 (GRCm39) missense probably damaging 0.99
R4606:Pla2g4f UTSW 2 120,144,467 (GRCm39) missense probably benign 0.00
R4685:Pla2g4f UTSW 2 120,135,496 (GRCm39) missense probably damaging 1.00
R4728:Pla2g4f UTSW 2 120,131,402 (GRCm39) missense probably benign 0.19
R4782:Pla2g4f UTSW 2 120,133,757 (GRCm39) missense probably damaging 1.00
R4957:Pla2g4f UTSW 2 120,130,980 (GRCm39) missense probably benign 0.28
R5781:Pla2g4f UTSW 2 120,135,504 (GRCm39) missense probably damaging 0.97
R6158:Pla2g4f UTSW 2 120,131,552 (GRCm39) missense probably benign 0.21
R6232:Pla2g4f UTSW 2 120,132,702 (GRCm39) missense possibly damaging 0.63
R6629:Pla2g4f UTSW 2 120,138,723 (GRCm39) missense probably damaging 1.00
R6894:Pla2g4f UTSW 2 120,134,077 (GRCm39) missense probably benign 0.44
R6939:Pla2g4f UTSW 2 120,137,782 (GRCm39) missense probably damaging 1.00
R7131:Pla2g4f UTSW 2 120,135,035 (GRCm39) missense probably null 0.01
R7221:Pla2g4f UTSW 2 120,131,476 (GRCm39) missense probably benign 0.06
R7421:Pla2g4f UTSW 2 120,137,737 (GRCm39) missense probably benign 0.07
R7767:Pla2g4f UTSW 2 120,135,490 (GRCm39) missense possibly damaging 0.87
R9389:Pla2g4f UTSW 2 120,132,781 (GRCm39) missense probably damaging 1.00
R9425:Pla2g4f UTSW 2 120,133,264 (GRCm39) missense possibly damaging 0.75
R9500:Pla2g4f UTSW 2 120,142,713 (GRCm39) critical splice acceptor site probably null
R9657:Pla2g4f UTSW 2 120,135,138 (GRCm39) missense probably benign
R9714:Pla2g4f UTSW 2 120,142,900 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- CTTCTGATGAAGCTGCTGCC -3'
(R):5'- GTACTCCAGCCTAGTCTTGC -3'

Sequencing Primer
(F):5'- GAGAGAGATACTGCCCCATCTTCTC -3'
(R):5'- GCTTTTCCCACTCCCAGGTG -3'
Posted On 2021-01-18