Mutagenetix > Incidental Mutation

Incidental Mutation 'R8468:Zfp937'

656904

Institutional Source

Beutler Lab

Gene Symbol

Zfp937

Ensembl Gene

ENSMUSG00000060336

Gene Name

zinc finger protein 937

Synonyms

Gm4979

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R8468 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

150218073-150240725 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 150238714 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 221 (D221E)

Ref Sequence

ENSEMBL: ENSMUSP00000073454 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073782]

AlphaFold

A2ANU7

Predicted Effect

probably benign
Transcript: ENSMUST00000073782
AA Change: D221E

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000073454
Gene: ENSMUSG00000060336
AA Change: D221E

Domain	Start	End	E-Value	Type
KRAB	4	66	8.19e-20	SMART
ZnF_C2H2	103	125	1.28e-3	SMART
ZnF_C2H2	131	153	2.53e-2	SMART
ZnF_C2H2	159	181	9.58e-3	SMART
ZnF_C2H2	187	209	2.09e-3	SMART
ZnF_C2H2	215	237	2.2e-2	SMART
ZnF_C2H2	243	265	2.2e-2	SMART
ZnF_C2H2	271	293	2.2e-2	SMART
ZnF_C2H2	299	321	1.82e-3	SMART
ZnF_C2H2	327	349	3.69e-4	SMART
ZnF_C2H2	355	377	4.47e-3	SMART
ZnF_C2H2	383	405	3.89e-3	SMART
ZnF_C2H2	411	433	4.79e-3	SMART
ZnF_C2H2	439	461	8.47e-4	SMART
ZnF_C2H2	467	490	7.26e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts1	A	G	16: 85,795,556	W655R	possibly damaging	Het
Adamts3	C	T	5: 89,694,768	A748T	probably benign	Het
Ano1	A	C	7: 144,655,620	F248C	probably damaging	Het
Ap2b1	T	A	11: 83,351,065	L628Q	probably damaging	Het
BC035947	C	A	1: 78,498,330	A522S	probably damaging	Het
Bdp1	A	G	13: 100,060,568	V1103A	probably benign	Het
Cd248	T	A	19: 5,069,882	I586N	possibly damaging	Het
Dnah9	C	A	11: 65,831,730	M4428I	probably benign	Het
Epha5	T	C	5: 84,142,416		probably null	Het
Fan1	T	A	7: 64,372,486	N340Y	probably damaging	Het
Fastkd2	T	A	1: 63,731,764	L93Q	probably benign	Het
Gm6665	T	C	18: 31,820,400	D5G	possibly damaging	Het
Gphn	T	C	12: 78,226,827	V17A	probably benign	Het
Gpr85	A	T	6: 13,836,296	L203H	probably damaging	Het
Grk6	A	G	13: 55,451,385	Y166C	probably damaging	Het
Ints3	A	G	3: 90,406,253	V356A	probably damaging	Het
Krt33b	G	A	11: 100,029,789	R13C	probably damaging	Het
Lgals3	A	G	14: 47,381,647	I146V	possibly damaging	Het
Lrp1	G	A	10: 127,558,650	R2565C	probably damaging	Het
Miip	G	T	4: 147,861,471	D325E	probably damaging	Het
Naaladl1	T	A	19: 6,108,585	V249E	probably damaging	Het
Nfxl1	C	T	5: 72,518,205	R811K	possibly damaging	Het
Olfr1145	T	C	2: 87,810,738	I306T	possibly damaging	Het
Olfr676	A	T	7: 105,035,746	I183F	probably damaging	Het
Olfr681	A	G	7: 105,121,478	D7G	probably benign	Het
Olfr701	A	G	7: 106,818,839	Y252C	possibly damaging	Het
Olfr822	C	T	10: 130,074,434	T8I	probably benign	Het
Olfr994	T	A	2: 85,430,178	Y217F	probably damaging	Het
Pkd2l2	A	G	18: 34,427,411	D357G	possibly damaging	Het
Ppp1r36	A	G	12: 76,436,205	Y189C	probably damaging	Het
Rev3l	A	G	10: 39,827,991	E2011G	probably damaging	Het
Sestd1	T	A	2: 77,191,746	T534S	probably benign	Het
Sfmbt1	G	A	14: 30,773,984	A75T	probably benign	Het
Smarcc2	G	A	10: 128,484,393	R882H	probably benign	Het
Son	CATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCTCCATGGACTCCCAGATGTTAGCAAC	CATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCTCCATGGACTCCCAGATGTTAGCAAC	16: 91,656,691		probably benign	Het
Speg	C	T	1: 75,431,309	A3216V	probably damaging	Het
Vmn1r12	A	G	6: 57,159,385	T112A	probably benign	Het

Other mutations in Zfp937

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0350:Zfp937	UTSW	2	150239302	missense	possibly damaging	0.91
R0449:Zfp937	UTSW	2	150239546	missense	probably benign	0.13
R1403:Zfp937	UTSW	2	150238948	nonsense	probably null
R1403:Zfp937	UTSW	2	150238948	nonsense	probably null
R1465:Zfp937	UTSW	2	150239047	nonsense	probably null
R1465:Zfp937	UTSW	2	150239047	nonsense	probably null
R4510:Zfp937	UTSW	2	150238511	missense	probably damaging	0.98
R4511:Zfp937	UTSW	2	150238511	missense	probably damaging	0.98
R4689:Zfp937	UTSW	2	150236786	missense	probably damaging	1.00
R5290:Zfp937	UTSW	2	150238309	nonsense	probably null
R6287:Zfp937	UTSW	2	150238341	missense	possibly damaging	0.89
R6701:Zfp937	UTSW	2	150239216	missense	probably damaging	1.00
R6746:Zfp937	UTSW	2	150239423	nonsense	probably null
R6838:Zfp937	UTSW	2	150239346	missense	probably benign	0.01
R7162:Zfp937	UTSW	2	150239519	missense	probably benign	0.35
R7213:Zfp937	UTSW	2	150239465	missense	probably damaging	1.00
R7441:Zfp937	UTSW	2	150238710	frame shift	probably null
R7481:Zfp937	UTSW	2	150239346	missense	probably benign	0.01
R7694:Zfp937	UTSW	2	150239348	missense	probably damaging	1.00
R7856:Zfp937	UTSW	2	150239547	missense	probably benign	0.23
R7902:Zfp937	UTSW	2	150238761	missense	probably damaging	1.00
R7956:Zfp937	UTSW	2	150239156	missense	probably benign	0.03
R8058:Zfp937	UTSW	2	150239501	missense	probably benign	0.00
R9514:Zfp937	UTSW	2	150238970	missense	possibly damaging	0.95
R9617:Zfp937	UTSW	2	150238532	missense	probably damaging	1.00
X0017:Zfp937	UTSW	2	150218161	splice site	probably null

Predicted Primers

PCR Primer
(F):5'- GAGTGGTCTCCAACAGCATAAAAG -3'
(R):5'- TGCAGACCACTTTGAAACCCAA -3'

Sequencing Primer
(F):5'- CTGTCATCTTCGAGGGCATCAAAG -3'
(R):5'- GTGCAAATGCCTTATCACACTGG -3'

Posted On

2021-01-18