Mutagenetix > Incidental Mutations

Incidental Mutation 'R8486:Olfr656'

657741

Institutional Source

Beutler Lab

Gene Symbol

Olfr656

Ensembl Gene

ENSMUSG00000073924

Gene Name

olfactory receptor 656

Synonyms

GA_x6K02T2PBJ9-7245486-7246451, MOR27-1

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R8486 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

104613955-104621603 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 104617862 bp

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 69 (V69A)

Ref Sequence

ENSEMBL: ENSMUSP00000095774 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098172] [ENSMUST00000215575]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000098172
AA Change: V69A

PolyPhen 2 Score 0.475 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000095774
Gene: ENSMUSG00000073924
AA Change: V69A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	41	319	5.2e-105	PFAM
Pfam:7TM_GPCR_Srsx	42	316	1.1e-8	PFAM
Pfam:7tm_1	51	302	4.9e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215575
AA Change: V61A

PolyPhen 2 Score 0.143 (Sensitivity: 0.92; Specificity: 0.86)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	G	11: 9,275,092	S524G	probably benign	Het
Abca8b	A	T	11: 109,967,111	I546N	possibly damaging	Het
Abcd4	C	T	12: 84,603,978	R532H	probably damaging	Het
Acsf2	A	T	11: 94,569,960	D373E	probably damaging	Het
Acsm1	A	G	7: 119,660,657	T510A	probably damaging	Het
Adam21	C	T	12: 81,560,776	V71I	probably benign	Het
Adgra3	T	C	5: 49,990,279	D473G	probably damaging	Het
Agbl2	G	A	2: 90,801,155	V286I	possibly damaging	Het
Alkbh8	A	G	9: 3,344,642	E141G	probably null	Het
Arhgap21	T	C	2: 20,860,425	D952G	probably damaging	Het
Asb4	A	T	6: 5,390,653	K15N	possibly damaging	Het
Atp2b4	CTTCTT	CTTCTTTTTCTT	1: 133,701,744		probably benign	Het
Clip1	G	T	5: 123,614,707		probably benign	Het
Clip4	T	C	17: 71,863,844		probably benign	Het
Col4a3bp	T	C	13: 96,634,182	V533A	probably damaging	Het
Cyp2b10	T	C	7: 25,914,881	V260A	probably benign	Het
Dnm1	C	T	2: 32,334,727	M430I	probably benign	Het
Dpp7	T	C	2: 25,352,549	D464G	probably damaging	Het
Dspp	A	G	5: 104,174,017		probably benign	Het
Ephb1	A	C	9: 101,963,965	D730E	probably benign	Het
Fancf	A	G	7: 51,861,884	V124A	probably benign	Het
Fgfr2	G	A	7: 130,164,015	P692S	possibly damaging	Het
Fkbp15	G	A	4: 62,312,284	R731*	probably null	Het
Fos	T	C	12: 85,476,031	F239S	probably benign	Het
Fuz	T	C	7: 44,898,668	Y107H	probably damaging	Het
Gm3159	A	C	14: 4,400,520	N193T	probably damaging	Het
Helz2	T	A	2: 181,229,331	D2771V	probably damaging	Het
Ighv1-18	T	A	12: 114,682,705	K92M	probably damaging	Het
Kcnh3	A	G	15: 99,238,210	Y696C	probably damaging	Het
Klf15	T	A	6: 90,467,178	V245D	probably damaging	Het
L1td1	C	T	4: 98,737,674	A702V	probably damaging	Het
Lamc2	A	G	1: 153,158,891	M122T	probably benign	Het
Lrp1b	A	G	2: 41,728,690	L124P	probably damaging	Het
Mcrs1	G	T	15: 99,248,468	Y85*	probably null	Het
Mgst1	A	T	6: 138,143,028	I29L	probably benign	Het
Miga1	C	T	3: 152,276,753	R562H	probably damaging	Het
Mybpc3	T	C	2: 91,128,772	W687R	probably damaging	Het
Myh11	G	T	16: 14,204,668	A1682D	possibly damaging	Het
Nes	T	C	3: 87,980,013	S1816P	probably damaging	Het
Olfr368	T	G	2: 37,332,650	V301G	probably damaging	Het
Pkhd1l1	T	G	15: 44,547,416	F2773C	probably damaging	Het
Pkp1	C	G	1: 135,918,976	E13Q	probably damaging	Het
Ppp2r2b	T	C	18: 42,898,804	D4G	probably benign	Het
Ppp6r3	G	A	19: 3,487,072	H455Y	probably benign	Het
R3hdm1	A	G	1: 128,178,920	R202G	probably benign	Het
Sgcz	T	G	8: 37,723,053	K106N	probably benign	Het
Slc13a4	T	C	6: 35,270,369	Y572C	probably damaging	Het
Syne2	C	T	12: 76,042,107	Q5080*	probably null	Het
Tpst2	C	T	5: 112,308,305	Q5*	probably null	Het
Ttn	T	A	2: 76,731,533	T28908S	possibly damaging	Het
Vmn1r120	C	T	7: 21,053,102	R228H	probably benign	Het
Vmn2r3	T	C	3: 64,278,949	N105S	probably damaging	Het
Zfp521	T	C	18: 13,846,772	T195A	probably damaging	Het
Zfp616	T	A	11: 74,084,083	S484T	probably benign	Het

Other mutations in Olfr656

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01616:Olfr656	APN	7	104617721	missense	probably damaging	0.98
IGL01908:Olfr656	APN	7	104617699	missense	probably damaging	1.00
IGL02695:Olfr656	APN	7	104618471	missense	probably damaging	1.00
IGL03055:Olfr656	UTSW	7	104618206	missense	probably damaging	1.00
R0128:Olfr656	UTSW	7	104618581	missense	probably damaging	1.00
R0184:Olfr656	UTSW	7	104618240	missense	probably damaging	1.00
R4674:Olfr656	UTSW	7	104618424	nonsense	probably null
R4675:Olfr656	UTSW	7	104618424	nonsense	probably null
R4723:Olfr656	UTSW	7	104618489	missense	possibly damaging	0.56
R4979:Olfr656	UTSW	7	104618605	missense	probably null	0.03
R6273:Olfr656	UTSW	7	104617895	missense	probably damaging	1.00
R6359:Olfr656	UTSW	7	104618303	missense	probably damaging	1.00
R6582:Olfr656	UTSW	7	104618441	missense	probably damaging	1.00
R6750:Olfr656	UTSW	7	104618113	missense	probably damaging	0.99
R7426:Olfr656	UTSW	7	104617852	missense	probably damaging	1.00
R7786:Olfr656	UTSW	7	104617718	missense	probably benign	0.02
R8068:Olfr656	UTSW	7	104618253	nonsense	probably null
R8324:Olfr656	UTSW	7	104618114	missense	probably benign	0.00
X0065:Olfr656	UTSW	7	104617758	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGTGGGATTTTCTGACCAGAGAC -3'
(R):5'- ACAGCACTGTGGACTCCATC -3'

Sequencing Primer
(F):5'- GGATTTTCTGACCAGAGACTATGTCC -3'
(R):5'- CTGTGGACTCCATCATGCAAAAGG -3'

Posted On

2021-01-18