Mutagenetix > Incidental Mutation

Incidental Mutation 'R8492:Miip'

658035

Institutional Source

Beutler Lab

Gene Symbol

Miip

Ensembl Gene

ENSMUSG00000029022

Gene Name

migration and invasion inhibitory protein

Synonyms

D4Wsu114e

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R8492 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

147860778-147868816 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 147861424 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 341 (D341G)

Ref Sequence

ENSEMBL: ENSMUSP00000134085 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030886] [ENSMUST00000119975] [ENSMUST00000172710]

AlphaFold

A2A7Y5

Predicted Effect

probably damaging
Transcript: ENSMUST00000030886
AA Change: D341G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000030886
Gene: ENSMUSG00000029022
AA Change: D341G

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000119975
AA Change: D341G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000113897
Gene: ENSMUSG00000029022
AA Change: D341G

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
Pfam:MIIP	41	382	1.4e-142	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000172710
AA Change: D341G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000134085
Gene: ENSMUSG00000029022
AA Change: D341G

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Meta Mutation Damage Score

0.1725

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (68/68)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that interacts with the oncogene protein insulin-like growth factor binding protein 2 and may function as an inhibitor of cell migration and invasion. This protein also interacts with the cell division protein 20 and may be involved in regulating mitotic progression. This protein may function as a tumor suppressor by inhibiting the growth or certain cancers. [provided by RefSeq, Sep 2011]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9330159F19Rik	G	A	10: 29,218,247	R43K	possibly damaging	Het
Abcc2	A	T	19: 43,804,971	Y354F	probably benign	Het
Aloxe3	A	T	11: 69,126,475	T25S	possibly damaging	Het
Als2	T	C	1: 59,211,344	K414E	probably damaging	Het
Ankmy2	A	T	12: 36,176,591	I95F	probably damaging	Het
Ankrd35	T	G	3: 96,682,213		probably null	Het
Ap3b1	A	G	13: 94,394,786	N91S	possibly damaging	Het
Apip	T	A	2: 103,092,521	L228H	probably damaging	Het
Arhgap23	A	G	11: 97,475,021	Y488C	probably damaging	Het
Asah2	G	A	19: 32,006,259	T595M	probably benign	Het
Ccdc174	G	C	6: 91,888,157	R132T	probably benign	Het
Ccng2	A	C	5: 93,271,454	H233P	probably damaging	Het
Cemip	A	G	7: 83,973,214	F586L	probably damaging	Het
Cenpf	A	G	1: 189,658,729	S969P	probably damaging	Het
Cep170b	G	A	12: 112,744,700	D1505N	probably damaging	Het
Colec12	T	A	18: 9,876,980		probably null	Het
Cytip	A	G	2: 58,137,857		probably null	Het
D630045J12Rik	A	T	6: 38,190,590	S1026T	probably damaging	Het
Disc1	T	A	8: 125,090,438	D372E	probably damaging	Het
Dlgap2	A	T	8: 14,778,271	M560L	possibly damaging	Het
Dync2li1	C	A	17: 84,649,706		probably null	Het
Eif3c	C	T	7: 126,563,110	G180D	probably damaging	Het
Fam35a	T	A	14: 34,245,232	K122N	probably damaging	Het
G530012D18Rik	C	G	1: 85,577,214	D113E	unknown	Het
G6pc2	A	G	2: 69,220,242	I70M	probably damaging	Het
Gm5145	A	T	17: 20,570,419	I20F	probably damaging	Het
Hba-x	T	A	11: 32,277,921	F128L	probably benign	Het
Hspb1	G	A	5: 135,889,368	E190K	possibly damaging	Het
Ift122	T	A	6: 115,887,005	S246T	probably benign	Het
Inppl1	A	T	7: 101,826,778	S828T	probably damaging	Het
Krt84	C	A	15: 101,529,616	Q301H	probably damaging	Het
Malrd1	T	G	2: 15,610,123	S250A		Het
Mettl21e	A	G	1: 44,206,393	I231T	probably damaging	Het
Ncoa1	T	C	12: 4,263,473	D1287G	probably damaging	Het
Ndufb5	T	G	3: 32,751,228		probably null	Het
Neb	T	C	2: 52,313,212	E309G	probably damaging	Het
Nf1	T	A	11: 79,408,422	F199I	probably benign	Het
Olfr360	T	A	2: 37,068,683	I126N	probably damaging	Het
Pcdhgc3	T	A	18: 37,807,294	Y249*	probably null	Het
Plekhg3	G	T	12: 76,576,016	V677L	probably benign	Het
Ppm1f	T	C	16: 16,915,178	Y25H	probably damaging	Het
Prok2	A	T	6: 99,714,476	H75Q	probably benign	Het
Ralgapa2	A	T	2: 146,342,604	H1494Q	possibly damaging	Het
Rapgef6	T	G	11: 54,690,237	I1277S	probably damaging	Het
Rhob	G	T	12: 8,499,531	Y34*	probably null	Het
Rnf141	T	G	7: 110,837,200	D7A	probably benign	Het
Robo1	T	C	16: 73,013,023	S1220P	probably benign	Het
Rpl13a	A	G	7: 45,126,521	V48A	possibly damaging	Het
Serpinb9f	C	A	13: 33,334,604	H362Q	probably damaging	Het
Slc22a8	G	T	19: 8,594,231	V109L	probably damaging	Het
Slc5a10	A	G	11: 61,673,983	V390A	probably benign	Het
Snx14	T	C	9: 88,381,816	N839D	possibly damaging	Het
Taf1c	G	A	8: 119,598,717	T802I	probably benign	Het
Tdh	C	A	14: 63,492,820	D337Y	probably damaging	Het
Tmem156	A	T	5: 65,065,095	Y255N	possibly damaging	Het
Tmpo	C	A	10: 91,161,858	R689L	probably benign	Het
Trim56	A	C	5: 137,112,929	C578G	probably benign	Het
Triobp	C	T	15: 78,994,126	H1750Y	possibly damaging	Het
Trpv3	G	A	11: 73,288,209	W481*	probably null	Het
Tssk3	T	C	4: 129,489,652	M76V	probably benign	Het
Vmn1r215	A	T	13: 23,075,886	Y32F	possibly damaging	Het
Vmn1r53	A	G	6: 90,223,412	I310T	possibly damaging	Het
Vmn2r112	A	G	17: 22,602,489	T148A	probably benign	Het
Zdhhc14	G	A	17: 5,712,414	V198I	probably damaging	Het
Zfp109	T	C	7: 24,228,074	R637G	possibly damaging	Het
Zic5	G	T	14: 122,465,062	Q86K	unknown	Het
Zzef1	T	G	11: 72,872,604	V1359G	probably damaging	Het
Zzef1	T	A	11: 72,886,746	M1801K	probably damaging	Het

Other mutations in Miip

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00531:Miip	APN	4	147865865	missense	probably damaging	1.00
IGL02134:Miip	APN	4	147865278	splice site	probably benign
IGL02829:Miip	APN	4	147863061	missense	probably benign	0.01
IGL03350:Miip	APN	4	147862522	missense	probably benign	0.01
R0200:Miip	UTSW	4	147862263	missense	probably damaging	0.99
R1647:Miip	UTSW	4	147865234	missense	probably benign	0.02
R1783:Miip	UTSW	4	147865774	missense	probably damaging	1.00
R1848:Miip	UTSW	4	147863092	missense	probably damaging	0.99
R1944:Miip	UTSW	4	147865965	missense	probably benign	0.15
R3615:Miip	UTSW	4	147865914	missense	probably benign	0.00
R3616:Miip	UTSW	4	147865914	missense	probably benign	0.00
R3882:Miip	UTSW	4	147861052	missense	possibly damaging	0.93
R4579:Miip	UTSW	4	147861061	missense	probably damaging	1.00
R5183:Miip	UTSW	4	147863069	missense	probably damaging	1.00
R6054:Miip	UTSW	4	147865678	missense	probably benign	0.00
R6056:Miip	UTSW	4	147862335	missense	probably damaging	1.00
R6304:Miip	UTSW	4	147863083	missense	probably benign	0.12
R6568:Miip	UTSW	4	147865915	missense	probably benign
R6603:Miip	UTSW	4	147865923	missense	possibly damaging	0.92
R7639:Miip	UTSW	4	147862564	missense	probably benign	0.22
R7701:Miip	UTSW	4	147862914	missense	probably null	0.86
R7795:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7796:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7797:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7872:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7920:Miip	UTSW	4	147862918	missense	probably benign	0.17
R8468:Miip	UTSW	4	147861471	missense	probably damaging	1.00
R8677:Miip	UTSW	4	147863046	missense	probably damaging	1.00
R8852:Miip	UTSW	4	147866382	start gained	probably benign
R8860:Miip	UTSW	4	147866382	start gained	probably benign
R9755:Miip	UTSW	4	147865862	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCAAGGAGTGTCAAAGTTACATGGG -3'
(R):5'- GTGGCCAGGTCAACACAAAC -3'

Sequencing Primer
(F):5'- TGGGACACCTTTGTCAGA -3'
(R):5'- CAAACTGAGTTTGGGGTGACC -3'

Posted On

2021-01-18