Mutagenetix > Incidental Mutation

Incidental Mutation 'R8499:Vmn2r61'

658415

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r61

Ensembl Gene

ENSMUSG00000090967

Gene Name

vomeronasal 2, receptor 61

Synonyms

Gprc2a-rs2, Casr-rs2, EG637873

MMRRC Submission

067941-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R8499 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41909477-41950179 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 41949700 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 707 (C707S)

Ref Sequence

ENSEMBL: ENSMUSP00000129576 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166131]

AlphaFold

L7N2B8

Predicted Effect

probably damaging
Transcript: ENSMUST00000166131
AA Change: C707S

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000129576
Gene: ENSMUSG00000090967
AA Change: C707S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	4e-42	PFAM
Pfam:NCD3G	514	567	1.9e-21	PFAM
Pfam:7tm_3	600	835	6.2e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (49/49)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam25	A	G	8: 41,208,189 (GRCm39)	D485G	probably damaging	Het
Ap4b1	G	A	3: 103,728,018 (GRCm39)	R344Q	probably damaging	Het
Apol7c	G	T	15: 77,410,280 (GRCm39)	P222Q	possibly damaging	Het
Atf6b	T	C	17: 34,869,796 (GRCm39)	L272P	probably damaging	Het
Atp11b	G	T	3: 35,864,854 (GRCm39)	R559I	probably benign	Het
Bhmt	T	A	13: 93,756,600 (GRCm39)	I343F	probably benign	Het
Btbd7	A	T	12: 102,754,631 (GRCm39)	F712I	probably damaging	Het
Card14	T	C	11: 119,222,070 (GRCm39)	L455P	probably benign	Het
Cast	A	T	13: 74,946,835 (GRCm39)	H26Q	probably benign	Het
Clcn1	A	G	6: 42,284,133 (GRCm39)	N567S	probably damaging	Het
Cnot6l	A	T	5: 96,225,176 (GRCm39)	W506R	probably damaging	Het
Dynap	T	A	18: 70,374,044 (GRCm39)	I161L	unknown	Het
Eif4g3	A	C	4: 137,893,239 (GRCm39)	T996P	probably damaging	Het
Exoc8	A	G	8: 125,623,849 (GRCm39)	Y173H	probably benign	Het
Fbll1	C	T	11: 35,688,907 (GRCm39)	V119M	probably damaging	Het
Fbxw22	A	T	9: 109,214,068 (GRCm39)	F249L	probably benign	Het
Fxyd1	C	A	7: 30,752,529 (GRCm39)		probably benign	Het
Grhl3	C	A	4: 135,276,549 (GRCm39)		probably null	Het
Grk4	A	G	5: 34,902,690 (GRCm39)	E414G	possibly damaging	Het
H2bc8	T	A	13: 23,755,880 (GRCm39)	S92T	probably benign	Het
H2-Q5	A	G	17: 35,613,820 (GRCm39)	D123G		Het
H2-Q5	C	T	17: 35,613,945 (GRCm39)	R165*	probably null	Het
Hgf	A	T	5: 16,771,854 (GRCm39)	E160D	probably damaging	Het
Ifi211	T	C	1: 173,733,086 (GRCm39)	I192V	probably benign	Het
Klk1b22	T	C	7: 43,762,144 (GRCm39)	F7L	probably benign	Het
Lcmt1	C	A	7: 123,029,371 (GRCm39)	T331N	probably benign	Het
Lrrc36	A	T	8: 106,176,168 (GRCm39)	T181S	possibly damaging	Het
Ltbp3	T	A	19: 5,798,712 (GRCm39)	S520T	probably benign	Het
Nacc1	A	T	8: 85,403,345 (GRCm39)	C177S	probably damaging	Het
Nedd4l	T	A	18: 65,342,728 (GRCm39)	V888E	probably damaging	Het
Oc90	C	T	15: 65,753,405 (GRCm39)	G305S	probably damaging	Het
Or51a25	A	G	7: 102,372,932 (GRCm39)	V255A	probably damaging	Het
Or52i2	T	A	7: 102,320,012 (GRCm39)	I295N	probably damaging	Het
Pappa2	T	A	1: 158,764,092 (GRCm39)	D473V	probably damaging	Het
Pou2f2	C	A	7: 24,799,623 (GRCm39)	G117C	probably damaging	Het
Prkci	A	G	3: 31,079,366 (GRCm39)	H68R	probably damaging	Het
Pygm	A	G	19: 6,440,392 (GRCm39)	K479E	probably damaging	Het
Qpct	A	G	17: 79,384,996 (GRCm39)	D212G	probably damaging	Het
Sh3glb2	A	T	2: 30,249,216 (GRCm39)	M1K	probably null	Het
Slc22a5	A	G	11: 53,758,469 (GRCm39)	S444P	probably damaging	Het
Snai3	G	A	8: 123,183,144 (GRCm39)	H134Y	probably benign	Het
Synpo	T	C	18: 60,736,044 (GRCm39)	D395G	probably damaging	Het
Tmem63c	C	T	12: 87,119,738 (GRCm39)	T344I	probably damaging	Het
Tnfsf14	A	G	17: 57,497,534 (GRCm39)	Y233H		Het
Ttn	A	G	2: 76,749,335 (GRCm39)	F3905L	probably benign	Het
Tubgcp5	T	A	7: 55,454,363 (GRCm39)	H219Q	possibly damaging	Het
Usp16	C	T	16: 87,271,536 (GRCm39)	T364M	possibly damaging	Het
Vmn1r59	C	T	7: 5,457,750 (GRCm39)	M3I	probably benign	Het
Vps13b	T	C	15: 35,841,466 (GRCm39)	S2499P	probably damaging	Het
Ythdf3	A	G	3: 16,259,179 (GRCm39)	E442G	possibly damaging	Het
Zfp1001	T	C	2: 150,204,907 (GRCm39)	S74P	probably benign	Het

Other mutations in Vmn2r61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r61	APN	7	41,950,175 (GRCm39)	missense	possibly damaging	0.96
IGL00824:Vmn2r61	APN	7	41,916,438 (GRCm39)	missense	probably benign	0.03
IGL00903:Vmn2r61	APN	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
IGL01125:Vmn2r61	APN	7	41,909,550 (GRCm39)	missense	probably damaging	1.00
IGL01393:Vmn2r61	APN	7	41,916,258 (GRCm39)	missense	probably benign	0.08
IGL01712:Vmn2r61	APN	7	41,909,661 (GRCm39)	missense	probably damaging	0.98
IGL01822:Vmn2r61	APN	7	41,950,130 (GRCm39)	missense	probably benign	0.18
IGL01835:Vmn2r61	APN	7	41,950,015 (GRCm39)	missense	probably benign	0.12
IGL01844:Vmn2r61	APN	7	41,909,639 (GRCm39)	missense	probably benign	0.00
IGL01953:Vmn2r61	APN	7	41,949,613 (GRCm39)	missense	probably damaging	1.00
IGL02032:Vmn2r61	APN	7	41,949,466 (GRCm39)	missense	probably damaging	0.99
IGL02054:Vmn2r61	APN	7	41,926,158 (GRCm39)	critical splice donor site	probably null
IGL02569:Vmn2r61	APN	7	41,926,070 (GRCm39)	missense	probably damaging	1.00
IGL02697:Vmn2r61	APN	7	41,924,892 (GRCm39)	missense	possibly damaging	0.55
IGL02958:Vmn2r61	APN	7	41,949,361 (GRCm39)	missense	probably benign
IGL03290:Vmn2r61	APN	7	41,915,408 (GRCm39)	missense	probably benign	0.00
IGL03337:Vmn2r61	APN	7	41,916,509 (GRCm39)	missense	possibly damaging	0.58
IGL03369:Vmn2r61	APN	7	41,909,517 (GRCm39)	missense	probably benign
IGL03402:Vmn2r61	APN	7	41,909,679 (GRCm39)	missense	probably benign
R0026:Vmn2r61	UTSW	7	41,924,898 (GRCm39)	missense	possibly damaging	0.64
R0319:Vmn2r61	UTSW	7	41,949,941 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r61	UTSW	7	41,915,335 (GRCm39)	missense	probably benign	0.02
R0555:Vmn2r61	UTSW	7	41,915,442 (GRCm39)	missense	probably benign	0.02
R0691:Vmn2r61	UTSW	7	41,949,844 (GRCm39)	missense	probably damaging	1.00
R1701:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R1718:Vmn2r61	UTSW	7	41,950,121 (GRCm39)	missense	probably benign
R1835:Vmn2r61	UTSW	7	41,916,076 (GRCm39)	nonsense	probably null
R1920:Vmn2r61	UTSW	7	41,949,710 (GRCm39)	missense	possibly damaging	0.73
R2069:Vmn2r61	UTSW	7	41,949,425 (GRCm39)	missense	probably benign	0.06
R2326:Vmn2r61	UTSW	7	41,916,287 (GRCm39)	missense	probably damaging	1.00
R2402:Vmn2r61	UTSW	7	41,949,529 (GRCm39)	missense	possibly damaging	0.90
R3103:Vmn2r61	UTSW	7	41,916,067 (GRCm39)	missense	possibly damaging	0.73
R3107:Vmn2r61	UTSW	7	41,916,491 (GRCm39)	missense	possibly damaging	0.82
R4426:Vmn2r61	UTSW	7	41,950,159 (GRCm39)	missense	probably benign
R4426:Vmn2r61	UTSW	7	41,950,157 (GRCm39)	missense	probably benign
R4484:Vmn2r61	UTSW	7	41,950,120 (GRCm39)	missense	probably benign
R4748:Vmn2r61	UTSW	7	41,916,565 (GRCm39)	missense	probably damaging	0.96
R4835:Vmn2r61	UTSW	7	41,916,459 (GRCm39)	missense	possibly damaging	0.52
R4863:Vmn2r61	UTSW	7	41,950,132 (GRCm39)	missense	probably benign	0.03
R4923:Vmn2r61	UTSW	7	41,916,520 (GRCm39)	missense	probably damaging	1.00
R4968:Vmn2r61	UTSW	7	41,949,478 (GRCm39)	missense	probably benign	0.14
R5114:Vmn2r61	UTSW	7	41,949,953 (GRCm39)	missense	possibly damaging	0.92
R5297:Vmn2r61	UTSW	7	41,909,646 (GRCm39)	missense	probably benign
R5497:Vmn2r61	UTSW	7	41,924,906 (GRCm39)	missense	possibly damaging	0.95
R5508:Vmn2r61	UTSW	7	41,916,242 (GRCm39)	missense	possibly damaging	0.52
R5587:Vmn2r61	UTSW	7	41,949,911 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,916,677 (GRCm39)	missense	probably benign	0.00
R5782:Vmn2r61	UTSW	7	41,949,253 (GRCm39)	missense	probably damaging	1.00
R6136:Vmn2r61	UTSW	7	41,916,455 (GRCm39)	missense	probably damaging	1.00
R6207:Vmn2r61	UTSW	7	41,909,616 (GRCm39)	missense	probably benign	0.01
R6265:Vmn2r61	UTSW	7	41,915,915 (GRCm39)	missense	probably benign	0.01
R6272:Vmn2r61	UTSW	7	41,949,242 (GRCm39)	missense	probably damaging	1.00
R6355:Vmn2r61	UTSW	7	41,916,659 (GRCm39)	missense	probably benign	0.00
R6469:Vmn2r61	UTSW	7	41,915,283 (GRCm39)	nonsense	probably null
R6554:Vmn2r61	UTSW	7	41,926,139 (GRCm39)	missense	probably damaging	1.00
R6699:Vmn2r61	UTSW	7	41,949,580 (GRCm39)	missense	probably benign
R6768:Vmn2r61	UTSW	7	41,949,748 (GRCm39)	missense	probably damaging	1.00
R6824:Vmn2r61	UTSW	7	41,949,403 (GRCm39)	missense	probably benign	0.10
R6930:Vmn2r61	UTSW	7	41,949,364 (GRCm39)	missense	probably benign	0.02
R7053:Vmn2r61	UTSW	7	41,916,557 (GRCm39)	missense	probably damaging	0.96
R7238:Vmn2r61	UTSW	7	41,916,629 (GRCm39)	missense	possibly damaging	0.73
R7332:Vmn2r61	UTSW	7	41,909,534 (GRCm39)	missense	probably benign	0.00
R7359:Vmn2r61	UTSW	7	41,915,407 (GRCm39)	missense	probably benign	0.11
R7553:Vmn2r61	UTSW	7	41,916,205 (GRCm39)	missense	not run
R7710:Vmn2r61	UTSW	7	41,916,472 (GRCm39)	missense	probably damaging	1.00
R7732:Vmn2r61	UTSW	7	41,916,097 (GRCm39)	missense	probably benign
R7839:Vmn2r61	UTSW	7	41,916,032 (GRCm39)	missense	probably damaging	0.97
R7916:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R8026:Vmn2r61	UTSW	7	41,916,141 (GRCm39)	missense	probably benign	0.02
R8440:Vmn2r61	UTSW	7	41,916,080 (GRCm39)	missense	probably benign	0.02
R8771:Vmn2r61	UTSW	7	41,916,194 (GRCm39)	missense	probably damaging	0.99
R8847:Vmn2r61	UTSW	7	41,950,010 (GRCm39)	missense	probably damaging	1.00
R8986:Vmn2r61	UTSW	7	41,915,325 (GRCm39)	nonsense	probably null
R9290:Vmn2r61	UTSW	7	41,915,385 (GRCm39)	missense	probably benign	0.27
R9311:Vmn2r61	UTSW	7	41,950,092 (GRCm39)	missense	possibly damaging	0.92
R9324:Vmn2r61	UTSW	7	41,916,619 (GRCm39)	missense	probably benign	0.00
R9476:Vmn2r61	UTSW	7	41,949,593 (GRCm39)	missense	probably damaging	1.00
R9521:Vmn2r61	UTSW	7	41,916,626 (GRCm39)	missense	probably damaging	0.99
R9619:Vmn2r61	UTSW	7	41,926,136 (GRCm39)	missense	probably damaging	0.98
R9729:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r61	UTSW	7	41,949,388 (GRCm39)	missense	possibly damaging	0.93
Z1176:Vmn2r61	UTSW	7	41,916,166 (GRCm39)	missense	probably benign	0.00
Z1176:Vmn2r61	UTSW	7	41,909,585 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- GACTCTCAGCTACATCCTGC -3'
(R):5'- AGCTCCCAAGTGCTAAGGAG -3'

Sequencing Primer
(F):5'- AGCTACATCCTGCTATTGACAC -3'
(R):5'- CTAAGGAGCAGAGGTACCCC -3'

Posted On

2021-01-18