Mutagenetix > Incidental Mutation

Incidental Mutation 'R8526:Zfp810'

658813

Institutional Source

Beutler Lab

Gene Symbol

Zfp810

Ensembl Gene

ENSMUSG00000066829

Gene Name

zinc finger protein 810

Synonyms

MMRRC Submission

067951-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.104) question?

Stock #

R8526 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

22188044-22218944 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 22189586 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 441 (K441E)

Ref Sequence

ENSEMBL: ENSMUSP00000083459 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086278] [ENSMUST00000215202]

AlphaFold

Q99K45

Predicted Effect

probably damaging
Transcript: ENSMUST00000086278
AA Change: K441E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000083459
Gene: ENSMUSG00000066829
AA Change: K441E

Domain	Start	End	E-Value	Type
KRAB	4	64	1.09e-33	SMART
ZnF_C2H2	126	148	2.44e2	SMART
ZnF_C2H2	182	204	3.07e-1	SMART
ZnF_C2H2	210	232	8.47e-4	SMART
ZnF_C2H2	238	260	6.78e-3	SMART
ZnF_C2H2	266	288	6.13e-1	SMART
ZnF_C2H2	294	316	5.06e-2	SMART
ZnF_C2H2	322	344	4.79e-3	SMART
ZnF_C2H2	350	372	2.99e-4	SMART
ZnF_C2H2	378	400	1.33e-1	SMART
ZnF_C2H2	406	428	2.75e-3	SMART
ZnF_C2H2	434	456	1.58e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000215202

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (42/42)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	T	A	13: 77,417,935 (GRCm39)	C706*	probably null	Het
4930522L14Rik	T	C	5: 109,885,655 (GRCm39)	I68V	possibly damaging	Het
4931422A03Rik	G	A	2: 103,856,499 (GRCm39)	R21C	unknown	Het
Actr5	T	G	2: 158,474,224 (GRCm39)	F342L	probably damaging	Het
Adamts13	T	C	2: 26,868,012 (GRCm39)	V124A	probably benign	Het
Amotl1	T	C	9: 14,473,492 (GRCm39)	E606G	probably damaging	Het
Carmil2	C	T	8: 106,415,447 (GRCm39)	A265V	probably damaging	Het
Cdh22	T	C	2: 164,954,178 (GRCm39)	E781G	probably damaging	Het
Dennd1b	G	A	1: 138,950,858 (GRCm39)	W33*	probably null	Het
Dlc1	C	T	8: 37,404,968 (GRCm39)	V274I	probably benign	Het
Dnase1l1	C	T	X: 73,320,644 (GRCm39)		probably null	Het
Ect2l	C	T	10: 18,020,375 (GRCm39)	E582K	probably benign	Het
Eppk1	C	T	15: 75,994,319 (GRCm39)	R854Q	probably benign	Het
Fbxl12	C	A	9: 20,550,160 (GRCm39)	R165L	possibly damaging	Het
Ift88	A	T	14: 57,683,126 (GRCm39)	K40*	probably null	Het
Igkv4-72	A	G	6: 69,204,140 (GRCm39)	V17A	probably benign	Het
Klhl28	T	G	12: 64,998,400 (GRCm39)	T365P	probably damaging	Het
Krt16	T	A	11: 100,137,309 (GRCm39)	Y434F	probably benign	Het
Lyst	T	A	13: 13,935,391 (GRCm39)	V3620E	probably damaging	Het
Mcm9	A	T	10: 53,506,221 (GRCm39)		probably benign	Het
Mef2a	T	C	7: 66,901,473 (GRCm39)	M100V	possibly damaging	Het
Mos	T	C	4: 3,871,709 (GRCm39)	K36E	probably damaging	Het
Ncapg2	T	C	12: 116,403,679 (GRCm39)	Y864H	probably benign	Het
Or1o2	A	G	17: 37,542,470 (GRCm39)	S264P	probably damaging	Het
Or4k2	C	T	14: 50,423,719 (GRCm39)		probably null	Het
Parp14	A	T	16: 35,661,307 (GRCm39)	I1547N	possibly damaging	Het
Pcdh18	A	T	3: 49,710,023 (GRCm39)	Y431N	probably damaging	Het
Phyhd1	T	C	2: 30,156,955 (GRCm39)		probably null	Het
Pkd2	T	C	5: 104,637,102 (GRCm39)	F572L	probably damaging	Het
Rasgrf1	T	A	9: 89,856,901 (GRCm39)	I453N	probably damaging	Het
Robo4	T	A	9: 37,314,801 (GRCm39)	C218*	probably null	Het
Rrbp1	A	G	2: 143,816,403 (GRCm39)	V742A	probably benign	Het
Serpinb3a	A	T	1: 106,976,504 (GRCm39)		probably null	Het
Sgsm2	T	G	11: 74,759,847 (GRCm39)	E97A	probably benign	Het
Smad4	A	T	18: 73,790,330 (GRCm39)		probably null	Het
Snd1	T	C	6: 28,745,253 (GRCm39)	Y533H	probably benign	Het
Sorbs1	G	C	19: 40,365,244 (GRCm39)	R180G	probably benign	Het
Stk36	A	G	1: 74,673,703 (GRCm39)	T1199A	probably benign	Het
Veph1	T	A	3: 66,066,737 (GRCm39)	N417I	probably benign	Het
Vmn1r8	A	G	6: 57,013,362 (GRCm39)	S138G	probably benign	Het
Vmn2r10	C	T	5: 109,145,572 (GRCm39)	V512I	possibly damaging	Het
Zfp740	A	G	15: 102,116,726 (GRCm39)	D47G	probably null	Het

Other mutations in Zfp810

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00484:Zfp810	APN	9	22,189,605 (GRCm39)	nonsense	probably null
IGL03079:Zfp810	APN	9	22,195,423 (GRCm39)	missense	probably damaging	1.00
IGL03402:Zfp810	APN	9	22,190,441 (GRCm39)	splice site	probably null
H8562:Zfp810	UTSW	9	22,190,387 (GRCm39)	missense	probably benign	0.42
R1116:Zfp810	UTSW	9	22,190,381 (GRCm39)	missense	probably benign	0.11
R1160:Zfp810	UTSW	9	22,189,828 (GRCm39)	missense	possibly damaging	0.64
R1171:Zfp810	UTSW	9	22,190,122 (GRCm39)	missense	possibly damaging	0.95
R1393:Zfp810	UTSW	9	22,191,810 (GRCm39)	missense	probably benign
R1608:Zfp810	UTSW	9	22,190,216 (GRCm39)	missense	probably benign	0.00
R1644:Zfp810	UTSW	9	22,190,324 (GRCm39)	missense	possibly damaging	0.67
R1766:Zfp810	UTSW	9	22,189,828 (GRCm39)	missense	possibly damaging	0.64
R2568:Zfp810	UTSW	9	22,190,534 (GRCm39)	missense	probably benign	0.01
R3684:Zfp810	UTSW	9	22,189,531 (GRCm39)	missense	probably benign	0.01
R4002:Zfp810	UTSW	9	22,190,188 (GRCm39)	missense	probably damaging	1.00
R4134:Zfp810	UTSW	9	22,190,369 (GRCm39)	missense	probably damaging	0.97
R4135:Zfp810	UTSW	9	22,190,369 (GRCm39)	missense	probably damaging	0.97
R4334:Zfp810	UTSW	9	22,190,080 (GRCm39)	missense	probably benign	0.00
R4545:Zfp810	UTSW	9	22,190,041 (GRCm39)	missense	probably damaging	0.96
R5399:Zfp810	UTSW	9	22,190,125 (GRCm39)	missense	possibly damaging	0.91
R5622:Zfp810	UTSW	9	22,190,392 (GRCm39)	missense	probably benign	0.00
R5643:Zfp810	UTSW	9	22,194,467 (GRCm39)	missense	probably benign	0.26
R7375:Zfp810	UTSW	9	22,201,833 (GRCm39)	critical splice donor site	probably null
R7441:Zfp810	UTSW	9	22,190,568 (GRCm39)	nonsense	probably null
R7809:Zfp810	UTSW	9	22,190,278 (GRCm39)	missense	possibly damaging	0.51
R8422:Zfp810	UTSW	9	22,194,518 (GRCm39)	nonsense	probably null
R8719:Zfp810	UTSW	9	22,190,571 (GRCm39)	missense	probably benign	0.00
R9177:Zfp810	UTSW	9	22,189,936 (GRCm39)	missense	probably damaging	1.00
R9479:Zfp810	UTSW	9	22,194,497 (GRCm39)	missense	possibly damaging	0.68
R9521:Zfp810	UTSW	9	22,190,227 (GRCm39)	missense	possibly damaging	0.81
R9683:Zfp810	UTSW	9	22,189,799 (GRCm39)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- ACCTTTCTTACATCAATGGCTTCTTA -3'
(R):5'- GCAAGTCTCACCTCACTGT -3'

Sequencing Primer
(F):5'- GATGCTAGTTAAGAACGGAC -3'
(R):5'- TGCAGGAAAGCTTTCTACCG -3'

Posted On

2021-01-18